CF504285

Overview
NameCF504285
Unique NameCF504285
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length390
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CF504285 vs. ExPASy Swiss-Prot
Match: METK_HAES1 (S-adenosylmethionine synthetase OS=Haemophilus somnus (strain 129Pt) GN=metK PE=3 SV=1)

HSP 1 Score: 79.337 bits (194), Expect = 3.269e-17
Identity = 38/70 (54.29%), Postives = 47/70 (67.14%), Query Frame = 1 
Query:  178 DKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLD 387
            DK+ DQ+SDAVLD  L QDP ++VACET  KT M +V GEITT A VD E + R     IG+  +D+G D
Sbjct:   17 DKIADQISDAVLDEILRQDPKARVACETYVKTGMALVGGEITTSAWVDIENLTRQVICDIGYKHSDIGFD 86

HSP 2 Score: 28.1054 bits (61), Expect = 3.269e-17
Identity = 11/15 (73.33%), Postives = 14/15 (93.33%), Query Frame = 3 
Query:  129 METFLFTSESVNEGH 173
            M ++LFTSESV+EGH
Sbjct:    1 MSSYLFTSESVSEGH 15
[Close]
BLAST of CF504285 vs. ExPASy Swiss-Prot
Match: METK_BUCAT (S-adenosylmethionine synthetase OS=Buchnera aphidicola subsp. Acyrthosiphon pisum (strain Tuc7) GN=metK PE=3 SV=1)

HSP 1 Score: 80.4925 bits (197), Expect = 3.270e-17
Identity = 39/71 (54.93%), Postives = 50/71 (70.42%), Query Frame = 1 
Query:  178 DKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLDA 390
            DK+ DQ+SDA+LD  L QD  ++VACET  KT MV++ GEITT A VD E+I R T   IG+ ++D G DA
Sbjct:   17 DKIADQISDALLDEILKQDLKARVACETYVKTGMVLIGGEITTTAWVDVEEITRKTINDIGYVNSDAGFDA 87

HSP 2 Score: 26.9498 bits (58), Expect = 3.270e-17
Identity = 11/15 (73.33%), Postives = 13/15 (86.67%), Query Frame = 3 
Query:  129 METFLFTSESVNEGH 173
            M  +LFTSESV+EGH
Sbjct:    1 MTEYLFTSESVSEGH 15
[Close]
BLAST of CF504285 vs. ExPASy Swiss-Prot
Match: METK_BUCAI (S-adenosylmethionine synthetase OS=Buchnera aphidicola subsp. Acyrthosiphon pisum GN=metK PE=3 SV=1)

HSP 1 Score: 80.4925 bits (197), Expect = 3.270e-17
Identity = 39/71 (54.93%), Postives = 50/71 (70.42%), Query Frame = 1 
Query:  178 DKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLDA 390
            DK+ DQ+SDA+LD  L QD  ++VACET  KT MV++ GEITT A VD E+I R T   IG+ ++D G DA
Sbjct:   17 DKIADQISDALLDEILKQDLKARVACETYVKTGMVLIGGEITTTAWVDVEEITRKTINDIGYVNSDAGFDA 87

HSP 2 Score: 26.9498 bits (58), Expect = 3.270e-17
Identity = 11/15 (73.33%), Postives = 13/15 (86.67%), Query Frame = 3 
Query:  129 METFLFTSESVNEGH 173
            M  +LFTSESV+EGH
Sbjct:    1 MTEYLFTSESVSEGH 15
[Close]
BLAST of CF504285 vs. ExPASy Swiss-Prot
Match: METK_BUCA5 (S-adenosylmethionine synthetase OS=Buchnera aphidicola subsp. Acyrthosiphon pisum (strain 5A) GN=metK PE=3 SV=1)

HSP 1 Score: 80.4925 bits (197), Expect = 3.270e-17
Identity = 39/71 (54.93%), Postives = 50/71 (70.42%), Query Frame = 1 
Query:  178 DKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLDA 390
            DK+ DQ+SDA+LD  L QD  ++VACET  KT MV++ GEITT A VD E+I R T   IG+ ++D G DA
Sbjct:   17 DKIADQISDALLDEILKQDLKARVACETYVKTGMVLIGGEITTTAWVDVEEITRKTINDIGYVNSDAGFDA 87

HSP 2 Score: 26.9498 bits (58), Expect = 3.270e-17
Identity = 11/15 (73.33%), Postives = 13/15 (86.67%), Query Frame = 3 
Query:  129 METFLFTSESVNEGH 173
            M  +LFTSESV+EGH
Sbjct:    1 MTEYLFTSESVSEGH 15
[Close]
BLAST of CF504285 vs. ExPASy Swiss-Prot
Match: METK_LACH4 (S-adenosylmethionine synthetase OS=Lactobacillus helveticus (strain DPC 4571) GN=metK PE=3 SV=1)

HSP 1 Score: 80.1073 bits (196), Expect = 4.246e-17
Identity = 38/70 (54.29%), Postives = 49/70 (70.00%), Query Frame = 1 
Query:  178 DKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLD 387
            DK+ DQ+SDA+LDA L +DP+S VACET   T MV VFGEI+T A VD + +VR T   IG+   ++G D
Sbjct:   17 DKVADQISDAILDAMLEKDPNSHVACETIVTTGMVFVFGEISTNAYVDIQDVVRKTILRIGYDRPELGFD 86

HSP 2 Score: 26.9498 bits (58), Expect = 4.246e-17
Identity = 12/15 (80.00%), Postives = 13/15 (86.67%), Query Frame = 3 
Query:  129 METFLFTSESVNEGH 173
            ME  LFTSESV+EGH
Sbjct:    1 MERRLFTSESVSEGH 15
[Close]
BLAST of CF504285 vs. ExPASy Swiss-Prot
Match: METK_THEP3 (S-adenosylmethionine synthetase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) GN=metK PE=3 SV=1)

HSP 1 Score: 82.8037 bits (203), Expect = 4.247e-17
Identity = 38/71 (53.52%), Postives = 49/71 (69.01%), Query Frame = 1 
Query:  178 DKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLDA 390
            DK+CDQ+SDA+LD  L +DP ++VACET   T +V+V GEITT+  VD  K+VR     IG+T A  G DA
Sbjct:   16 DKICDQISDAILDEILKKDPYARVACETAVTTGLVLVMGEITTQCYVDIPKVVRKVVEEIGYTRAKFGFDA 86

HSP 2 Score: 24.2534 bits (51), Expect = 4.247e-17
Identity = 10/11 (90.91%), Postives = 10/11 (90.91%), Query Frame = 3 
Query:  141 LFTSESVNEGH 173
            LFTSESV EGH
Sbjct:    4 LFTSESVTEGH 14
[Close]
BLAST of CF504285 vs. ExPASy Swiss-Prot
Match: METK_STRSY (S-adenosylmethionine synthetase OS=Streptococcus suis (strain 05ZYH33) GN=metK PE=3 SV=1)

HSP 1 Score: 82.0333 bits (201), Expect = 4.247e-17
Identity = 39/71 (54.93%), Postives = 49/71 (69.01%), Query Frame = 1 
Query:  178 DKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLDA 390
            DK+ DQ+SDA+LDA LA+DPD+ VA ET   T  V VFGEI+T A VD  ++VRDT   IG+T  + G  A
Sbjct:   18 DKIADQISDAILDAILAEDPDAHVAAETAVYTGSVHVFGEISTTAYVDINRVVRDTIAEIGYTKGEYGFSA 88

HSP 2 Score: 25.0238 bits (53), Expect = 4.247e-17
Identity = 11/14 (78.57%), Postives = 12/14 (85.71%), Query Frame = 3 
Query:  132 ETFLFTSESVNEGH 173
            E  LFTSESV+EGH
Sbjct:    3 ERKLFTSESVSEGH 16
[Close]
BLAST of CF504285 vs. ExPASy Swiss-Prot
Match: METK_STRS2 (S-adenosylmethionine synthetase OS=Streptococcus suis (strain 98HAH33) GN=metK PE=3 SV=1)

HSP 1 Score: 82.0333 bits (201), Expect = 4.247e-17
Identity = 39/71 (54.93%), Postives = 49/71 (69.01%), Query Frame = 1 
Query:  178 DKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLDA 390
            DK+ DQ+SDA+LDA LA+DPD+ VA ET   T  V VFGEI+T A VD  ++VRDT   IG+T  + G  A
Sbjct:   18 DKIADQISDAILDAILAEDPDAHVAAETAVYTGSVHVFGEISTTAYVDINRVVRDTIAEIGYTKGEYGFSA 88

HSP 2 Score: 25.0238 bits (53), Expect = 4.247e-17
Identity = 11/14 (78.57%), Postives = 12/14 (85.71%), Query Frame = 3 
Query:  132 ETFLFTSESVNEGH 173
            E  LFTSESV+EGH
Sbjct:    3 ERKLFTSESVSEGH 16
[Close]
BLAST of CF504285 vs. ExPASy Swiss-Prot
Match: METK_BURXL (S-adenosylmethionine synthetase OS=Burkholderia xenovorans (strain LB400) GN=metK PE=3 SV=1)

HSP 1 Score: 81.2629 bits (199), Expect = 4.247e-17
Identity = 38/70 (54.29%), Postives = 52/70 (74.29%), Query Frame = 1 
Query:  178 DKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLD 387
            DK+ DQ+SDA+LDA LAQD  S+VA ET   T +V++ GEITT ANVDY ++ R+T + IG+ + D G+D
Sbjct:   18 DKVADQISDAILDAILAQDKYSRVAAETLCNTGLVVLAGEITTTANVDYIQVARNTIKRIGYDNTDYGID 87

HSP 2 Score: 25.7942 bits (55), Expect = 4.247e-17
Identity = 10/12 (83.33%), Postives = 12/12 (100.00%), Query Frame = 3 
Query:  138 FLFTSESVNEGH 173
            +LFTSESV+EGH
Sbjct:    5 YLFTSESVSEGH 16
[Close]
BLAST of CF504285 vs. ExPASy Swiss-Prot
Match: METK_BURS3 (S-adenosylmethionine synthetase OS=Burkholderia sp. (strain 383) GN=metK PE=3 SV=1)

HSP 1 Score: 81.2629 bits (199), Expect = 4.247e-17
Identity = 38/70 (54.29%), Postives = 51/70 (72.86%), Query Frame = 1 
Query:  178 DKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLD 387
            DK+ DQ+SDA+LDA L QD  S+VA ET   T +V++ GEITT AN+DY +I RDT + IG+ + D G+D
Sbjct:   18 DKVADQISDAILDAILEQDKYSRVAAETLCNTGLVVLAGEITTTANIDYIQIARDTIKRIGYDNTDYGID 87

HSP 2 Score: 25.7942 bits (55), Expect = 4.247e-17
Identity = 10/12 (83.33%), Postives = 12/12 (100.00%), Query Frame = 3 
Query:  138 FLFTSESVNEGH 173
            +LFTSESV+EGH
Sbjct:    5 YLFTSESVSEGH 16
The following BLAST results are available for this feature:
BLAST of CF504285 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
METK_HAES13.269e-1754.29S-adenosylmethionine synthetase OS=Haemophilus som... [more]
METK_BUCAT3.270e-1754.93S-adenosylmethionine synthetase OS=Buchnera aphidi... [more]
METK_BUCAI3.270e-1754.93S-adenosylmethionine synthetase OS=Buchnera aphidi... [more]
METK_BUCA53.270e-1754.93S-adenosylmethionine synthetase OS=Buchnera aphidi... [more]
METK_LACH44.246e-1754.29S-adenosylmethionine synthetase OS=Lactobacillus h... [more]
METK_THEP34.247e-1753.52S-adenosylmethionine synthetase OS=Thermoanaerobac... [more]
METK_STRSY4.247e-1754.93S-adenosylmethionine synthetase OS=Streptococcus s... [more]
METK_STRS24.247e-1754.93S-adenosylmethionine synthetase OS=Streptococcus s... [more]
METK_BURXL4.247e-1754.29S-adenosylmethionine synthetase OS=Burkholderia xe... [more]
METK_BURS34.247e-1754.29S-adenosylmethionine synthetase OS=Burkholderia sp... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_119000-774 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-16_E09 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF504285 ID=CF504285; Name=CF504285; organism=Citrus sinensis; type=EST; length=390bp
CGTCTTTCTCTCGTTCTTCTGGGTACTTACCATTTTCGTATATCACCACC
TTCAATTCCATTTGTACTCGCACCACGGCCTCCTGCAAAGCGAAAGCTGC
AGCTTCAGGTTAAATCAAATTAAAGAAAATGGAAACATTTCTTTTCACAT
CAGAATCAGTGAACGAGGGTCACCTCGGACAAGCTGTGTGACCAAGTTTC
GGACGCAGTACTGGACGCGTGTTTGGCTCAGGATCCTGACAGCAAAGTTG
CTTGTGAAACATGCACAAAGACCAACATGGTCATGGTTTTTGGAGAAATT
ACAACCAAGGCCAATGTGGATTACGAGAAAATCGTGCGTGATACATGCAG
ATCGATTGGGTTCACATCGGCCGATGTGGGGCTTGATGCT
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