CF504285 | Citrus Genome Database

Overview


Name	CF504285
Unique Name	CF504285
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	390

Libraries


Library Name	Type
Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

BLAST of CF504285 vs. ExPASy Swiss-Prot
Match: METK_BURPP (S-adenosylmethionine synthetase OS=Burkholderia phytofirmans (strain DSM 17436 / PsJN) GN=metK PE=3 SV=1)

HSP 1 Score: 81.2629 bits (199), Expect = 4.247e-17
Identity = 38/70 (54.29%), Postives = 52/70 (74.29%), Query Frame = 1

Query:  178 DKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLD 387
            DK+ DQ+SDA+LDA LAQD  S+VA ET   T +V++ GEITT ANVDY ++ R+T + IG+ + D G+D
Sbjct:   18 DKVADQISDAILDAILAQDKYSRVAAETLCNTGLVVLAGEITTTANVDYIQVARNTIKRIGYDNTDYGID 87

HSP 2 Score: 25.7942 bits (55), Expect = 4.247e-17
Identity = 10/12 (83.33%), Postives = 12/12 (100.00%), Query Frame = 3

Query:  138 FLFTSESVNEGH 173
            +LFTSESV+EGH
Sbjct:    5 YLFTSESVSEGH 16

BLAST of CF504285 vs. ExPASy Swiss-Prot
Match: METK_BURCM (S-adenosylmethionine synthetase OS=Burkholderia ambifaria (strain ATCC BAA-244 / AMMD) GN=metK PE=3 SV=1)

HSP 1 Score: 81.2629 bits (199), Expect = 4.247e-17
Identity = 38/70 (54.29%), Postives = 51/70 (72.86%), Query Frame = 1

Query:  178 DKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLD 387
            DK+ DQ+SDA+LDA L QD  S+VA ET   T +V++ GEITT AN+DY +I RDT + IG+ + D G+D
Sbjct:   18 DKVADQISDAILDAILEQDKYSRVAAETLCNTGLVVLAGEITTTANIDYIQIARDTIKRIGYDNTDYGID 87

HSP 2 Score: 25.7942 bits (55), Expect = 4.247e-17
Identity = 10/12 (83.33%), Postives = 12/12 (100.00%), Query Frame = 3

Query:  138 FLFTSESVNEGH 173
            +LFTSESV+EGH
Sbjct:    5 YLFTSESVSEGH 16

BLAST of CF504285 vs. ExPASy Swiss-Prot
Match: METK_BURCJ (S-adenosylmethionine synthetase OS=Burkholderia cepacia (strain J2315 / LMG 16656) GN=metK PE=3 SV=1)

HSP 1 Score: 81.2629 bits (199), Expect = 4.247e-17
Identity = 38/70 (54.29%), Postives = 51/70 (72.86%), Query Frame = 1

Query:  178 DKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLD 387
            DK+ DQ+SDA+LDA L QD  S+VA ET   T +V++ GEITT AN+DY +I RDT + IG+ + D G+D
Sbjct:   18 DKVADQISDAILDAILEQDKYSRVAAETLCNTGLVVLAGEITTTANIDYIQIARDTIKRIGYDNTDYGID 87

HSP 2 Score: 25.7942 bits (55), Expect = 4.247e-17
Identity = 10/12 (83.33%), Postives = 12/12 (100.00%), Query Frame = 3

Query:  138 FLFTSESVNEGH 173
            +LFTSESV+EGH
Sbjct:    5 YLFTSESVSEGH 16

BLAST of CF504285 vs. ExPASy Swiss-Prot
Match: METK_BURCH (S-adenosylmethionine synthetase OS=Burkholderia cenocepacia (strain HI2424) GN=metK PE=3 SV=1)

HSP 1 Score: 81.2629 bits (199), Expect = 4.247e-17
Identity = 38/70 (54.29%), Postives = 51/70 (72.86%), Query Frame = 1

Query:  178 DKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLD 387
            DK+ DQ+SDA+LDA L QD  S+VA ET   T +V++ GEITT AN+DY +I RDT + IG+ + D G+D
Sbjct:   18 DKVADQISDAILDAILEQDKYSRVAAETLCNTGLVVLAGEITTTANIDYIQIARDTIKRIGYDNTDYGID 87

HSP 2 Score: 25.7942 bits (55), Expect = 4.247e-17
Identity = 10/12 (83.33%), Postives = 12/12 (100.00%), Query Frame = 3

Query:  138 FLFTSESVNEGH 173
            +LFTSESV+EGH
Sbjct:    5 YLFTSESVSEGH 16

BLAST of CF504285 vs. ExPASy Swiss-Prot
Match: METK_BURCC (S-adenosylmethionine synthetase OS=Burkholderia cenocepacia (strain MC0-3) GN=metK PE=3 SV=1)

HSP 1 Score: 81.2629 bits (199), Expect = 4.247e-17
Identity = 38/70 (54.29%), Postives = 51/70 (72.86%), Query Frame = 1

Query:  178 DKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLD 387
            DK+ DQ+SDA+LDA L QD  S+VA ET   T +V++ GEITT AN+DY +I RDT + IG+ + D G+D
Sbjct:   18 DKVADQISDAILDAILEQDKYSRVAAETLCNTGLVVLAGEITTTANIDYIQIARDTIKRIGYDNTDYGID 87

HSP 2 Score: 25.7942 bits (55), Expect = 4.247e-17
Identity = 10/12 (83.33%), Postives = 12/12 (100.00%), Query Frame = 3

Query:  138 FLFTSESVNEGH 173
            +LFTSESV+EGH
Sbjct:    5 YLFTSESVSEGH 16

BLAST of CF504285 vs. ExPASy Swiss-Prot
Match: METK_BURCA (S-adenosylmethionine synthetase OS=Burkholderia cenocepacia (strain AU 1054) GN=metK PE=3 SV=1)

HSP 1 Score: 81.2629 bits (199), Expect = 4.247e-17
Identity = 38/70 (54.29%), Postives = 51/70 (72.86%), Query Frame = 1

Query:  178 DKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLD 387
            DK+ DQ+SDA+LDA L QD  S+VA ET   T +V++ GEITT AN+DY +I RDT + IG+ + D G+D
Sbjct:   18 DKVADQISDAILDAILEQDKYSRVAAETLCNTGLVVLAGEITTTANIDYIQIARDTIKRIGYDNTDYGID 87

HSP 2 Score: 25.7942 bits (55), Expect = 4.247e-17
Identity = 10/12 (83.33%), Postives = 12/12 (100.00%), Query Frame = 3

Query:  138 FLFTSESVNEGH 173
            +LFTSESV+EGH
Sbjct:    5 YLFTSESVSEGH 16

BLAST of CF504285 vs. ExPASy Swiss-Prot
Match: METK_BURA4 (S-adenosylmethionine synthetase OS=Burkholderia ambifaria (strain MC40-6) GN=metK PE=3 SV=1)

HSP 1 Score: 81.2629 bits (199), Expect = 4.247e-17
Identity = 38/70 (54.29%), Postives = 51/70 (72.86%), Query Frame = 1

Query:  178 DKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLD 387
            DK+ DQ+SDA+LDA L QD  S+VA ET   T +V++ GEITT AN+DY +I RDT + IG+ + D G+D
Sbjct:   18 DKVADQISDAILDAILEQDKYSRVAAETLCNTGLVVLAGEITTTANIDYIQIARDTIKRIGYDNTDYGID 87

HSP 2 Score: 25.7942 bits (55), Expect = 4.247e-17
Identity = 10/12 (83.33%), Postives = 12/12 (100.00%), Query Frame = 3

Query:  138 FLFTSESVNEGH 173
            +LFTSESV+EGH
Sbjct:    5 YLFTSESVSEGH 16

BLAST of CF504285 vs. ExPASy Swiss-Prot
Match: METK_RALSO (S-adenosylmethionine synthetase OS=Ralstonia solanacearum GN=metK PE=3 SV=1)

HSP 1 Score: 80.1073 bits (196), Expect = 4.247e-17
Identity = 37/70 (52.86%), Postives = 51/70 (72.86%), Query Frame = 1

Query:  178 DKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLD 387
            DK+ DQ+SDA+LDA LAQD  ++VA ET   T +V++ GEITT ANVDY  + R+T + IG+ + D G+D
Sbjct:   18 DKVADQISDAILDAILAQDKYARVAAETLCNTGLVVLAGEITTSANVDYIHVARETIKRIGYDNTDYGID 87

HSP 2 Score: 26.9498 bits (58), Expect = 4.247e-17
Identity = 11/12 (91.67%), Postives = 12/12 (100.00%), Query Frame = 3

Query:  138 FLFTSESVNEGH 173
            FLFTSESV+EGH
Sbjct:    5 FLFTSESVSEGH 16

BLAST of CF504285 vs. ExPASy Swiss-Prot
Match: METK_WIGBR (S-adenosylmethionine synthetase OS=Wigglesworthia glossinidia brevipalpis GN=metK PE=3 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 4.249e-17
Identity = 34/71 (47.89%), Postives = 53/71 (74.65%), Query Frame = 1

Query:  178 DKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLDA 390
            DK+ DQ+SD +LDA L QD  ++VACET  KT MV+V GE+ + A +D E+++R+T + IG+ ++++G DA
Sbjct:   17 DKIADQISDTILDAILTQDKKARVACETYIKTGMVLVGGEVNSYAKIDIEELIRNTIKDIGYINSNMGFDA 87

HSP 2 Score: 27.335 bits (59), Expect = 4.249e-17
Identity = 11/15 (73.33%), Postives = 13/15 (86.67%), Query Frame = 3

Query:  129 METFLFTSESVNEGH 173
            M  +LFTSESV+EGH
Sbjct:    1 MNQYLFTSESVSEGH 15

BLAST of CF504285 vs. ExPASy Swiss-Prot
Match: METK_HAEPS (S-adenosylmethionine synthetase OS=Haemophilus parasuis serovar 5 (strain SH0165) GN=metK PE=3 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 4.250e-17
Identity = 40/71 (56.34%), Postives = 49/71 (69.01%), Query Frame = 1

Query:  178 DKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLDA 390
            DK+ DQ+SDAVLD  L QDP ++VACET  KT M +V GEITT A VD E + R     IG+T +D+G DA
Sbjct:   17 DKIADQISDAVLDEILKQDPKARVACETYVKTGMALVGGEITTSAWVDIENLTRQVICDIGYTHSDMGFDA 87

HSP 2 Score: 24.6386 bits (52), Expect = 4.250e-17
Identity = 10/11 (90.91%), Postives = 11/11 (100.00%), Query Frame = 3

Query:  141 LFTSESVNEGH 173
            LFTSESV+EGH
Sbjct:    5 LFTSESVSEGH 15

The following BLAST results are available for this feature:

BLAST of CF504285 vs. ExPASy Swiss-Prot

BLAST of CF504285 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500


Match Name	E-value	Identity	Description
METK_BURPP	4.247e-17	54.29	S-adenosylmethionine synthetase OS=Burkholderia ph...	[more]
METK_BURCM	4.247e-17	54.29	S-adenosylmethionine synthetase OS=Burkholderia am...	[more]
METK_BURCJ	4.247e-17	54.29	S-adenosylmethionine synthetase OS=Burkholderia ce...	[more]
METK_BURCH	4.247e-17	54.29	S-adenosylmethionine synthetase OS=Burkholderia ce...	[more]
METK_BURCC	4.247e-17	54.29	S-adenosylmethionine synthetase OS=Burkholderia ce...	[more]
METK_BURCA	4.247e-17	54.29	S-adenosylmethionine synthetase OS=Burkholderia ce...	[more]
METK_BURA4	4.247e-17	54.29	S-adenosylmethionine synthetase OS=Burkholderia am...	[more]
METK_RALSO	4.247e-17	52.86	S-adenosylmethionine synthetase OS=Ralstonia solan...	[more]
METK_WIGBR	4.249e-17	47.89	S-adenosylmethionine synthetase OS=Wigglesworthia ...	[more]
METK_HAEPS	4.250e-17	56.34	S-adenosylmethionine synthetase OS=Haemophilus par...	[more]

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Properties


Property Name	Value
Genbank description	USDA-FP_119000-774 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-16_E09 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CF504285 ID=CF504285; Name=CF504285; organism=Citrus sinensis; type=EST; length=390bp
CGTCTTTCTCTCGTTCTTCTGGGTACTTACCATTTTCGTATATCACCACC
TTCAATTCCATTTGTACTCGCACCACGGCCTCCTGCAAAGCGAAAGCTGC
AGCTTCAGGTTAAATCAAATTAAAGAAAATGGAAACATTTCTTTTCACAT
CAGAATCAGTGAACGAGGGTCACCTCGGACAAGCTGTGTGACCAAGTTTC
GGACGCAGTACTGGACGCGTGTTTGGCTCAGGATCCTGACAGCAAAGTTG
CTTGTGAAACATGCACAAAGACCAACATGGTCATGGTTTTTGGAGAAATT
ACAACCAAGGCCAATGTGGATTACGAGAAAATCGTGCGTGATACATGCAG
ATCGATTGGGTTCACATCGGCCGATGTGGGGCTTGATGCT