CF504285 | Citrus Genome Database

Overview


Name	CF504285
Unique Name	CF504285
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	390

Libraries


Library Name	Type
Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of CF504285 vs. ExPASy Swiss-Prot
Match: METK_ACTPJ (S-adenosylmethionine synthetase OS=Actinobacillus pleuropneumoniae serotype 3 (strain JL03) GN=metK PE=3 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 4.250e-17
Identity = 40/71 (56.34%), Postives = 49/71 (69.01%), Query Frame = 1

Query:  178 DKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLDA 390
            DK+ DQ+SDAVLD  L QDP ++VACET  KT M +V GEITT A VD E + R     IG+T +D+G DA
Sbjct:   17 DKIADQISDAVLDEILKQDPKARVACETYVKTGMALVGGEITTSAWVDIENLTRQVICDIGYTHSDMGFDA 87

HSP 2 Score: 24.6386 bits (52), Expect = 4.250e-17
Identity = 10/11 (90.91%), Postives = 11/11 (100.00%), Query Frame = 3

Query:  141 LFTSESVNEGH 173
            LFTSESV+EGH
Sbjct:    5 LFTSESVSEGH 15

[Close]

BLAST of CF504285 vs. ExPASy Swiss-Prot
Match: METK_ACTP7 (S-adenosylmethionine synthetase OS=Actinobacillus pleuropneumoniae serotype 7 (strain AP76) GN=metK PE=3 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 4.250e-17
Identity = 40/71 (56.34%), Postives = 49/71 (69.01%), Query Frame = 1

Query:  178 DKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLDA 390
            DK+ DQ+SDAVLD  L QDP ++VACET  KT M +V GEITT A VD E + R     IG+T +D+G DA
Sbjct:   17 DKIADQISDAVLDEILKQDPKARVACETYVKTGMALVGGEITTSAWVDIENLTRQVICDIGYTHSDMGFDA 87

HSP 2 Score: 24.6386 bits (52), Expect = 4.250e-17
Identity = 10/11 (90.91%), Postives = 11/11 (100.00%), Query Frame = 3

Query:  141 LFTSESVNEGH 173
            LFTSESV+EGH
Sbjct:    5 LFTSESVSEGH 15

[Close]

BLAST of CF504285 vs. ExPASy Swiss-Prot
Match: METK_ACTP2 (S-adenosylmethionine synthetase OS=Actinobacillus pleuropneumoniae serotype 5b (strain L20) GN=metK PE=3 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 4.250e-17
Identity = 40/71 (56.34%), Postives = 49/71 (69.01%), Query Frame = 1

Query:  178 DKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLDA 390
            DK+ DQ+SDAVLD  L QDP ++VACET  KT M +V GEITT A VD E + R     IG+T +D+G DA
Sbjct:   17 DKIADQISDAVLDEILKQDPKARVACETYVKTGMALVGGEITTSAWVDIENLTRQVICDIGYTHSDMGFDA 87

HSP 2 Score: 24.6386 bits (52), Expect = 4.250e-17
Identity = 10/11 (90.91%), Postives = 11/11 (100.00%), Query Frame = 3

Query:  141 LFTSESVNEGH 173
            LFTSESV+EGH
Sbjct:    5 LFTSESVSEGH 15

[Close]

BLAST of CF504285 vs. ExPASy Swiss-Prot
Match: METK_DESMR (S-adenosylmethionine synthetase OS=Desulfovibrio magneticus (strain ATCC 700980 / DSM 13731 / RS-1) GN=metK PE=3 SV=1)

HSP 1 Score: 86.2705 bits (212), Expect = 5.333e-17
Identity = 42/87 (48.28%), Postives = 58/87 (66.67%), Query Frame = 1

Query:  127 KWKHFFSHQNQ*TRVTSDKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLD 387
            K ++ FS ++  T    DK+ DQ+SD +LDA LAQDPD+ VACET   T +  + GEITTKA  D+  IVR+T + IG+ S+ +G D
Sbjct:    5 KGRYLFSSESV-TEGHPDKVADQISDGILDAILAQDPDAHVACETLVTTGLAFIAGEITTKAYADFPSIVRETVKEIGYNSSTMGFD 90

[Close]

BLAST of CF504285 vs. ExPASy Swiss-Prot
Match: METK_BIFLI (S-adenosylmethionine synthetase OS=Bifidobacterium longum subsp. infantis (strain ATCC 15697 / DSM 20088 / NCTC 11817 / S12) GN=metK PE=3 SV=1)

HSP 1 Score: 86.2705 bits (212), Expect = 5.333e-17
Identity = 40/71 (56.34%), Postives = 53/71 (74.65%), Query Frame = 1

Query:  178 DKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLDA 390
            DK+CDQ+SDA+LD  L QDP S VA ET   T + +VFGE+T+K   D +  VR+T R+IG+TS++VGLDA
Sbjct:   19 DKVCDQISDAILDDLLVQDPSSHVAVETSAATGVFLVFGEVTSKGYCDVQSKVRETLRNIGYTSSEVGLDA 89

[Close]

BLAST of CF504285 vs. ExPASy Swiss-Prot
Match: METK_BACSK (S-adenosylmethionine synthetase OS=Bacillus clausii (strain KSM-K16) GN=metK PE=3 SV=1)

HSP 1 Score: 86.2705 bits (212), Expect = 5.333e-17
Identity = 41/89 (46.07%), Postives = 59/89 (66.29%), Query Frame = 1

Query:  124 RKWKHFFSHQNQ*TRVTSDKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLDA 390
            ++ +H F+ ++  T    DK+CDQ+SDA+LD  L +DP+++VACET   T +V+V GEITT   VD   +VR T + IG+T A  G DA
Sbjct:    4 KRGRHLFTSESV-TEGHPDKICDQISDAILDEILKKDPNARVACETSVTTGLVLVAGEITTNTYVDIPSVVRQTIKGIGYTRAKYGFDA 91

[Close]

BLAST of CF504285 vs. ExPASy Swiss-Prot
Match: METK_STRMK (S-adenosylmethionine synthetase OS=Stenotrophomonas maltophilia (strain K279a) GN=metK PE=3 SV=1)

HSP 1 Score: 78.5666 bits (192), Expect = 5.518e-17
Identity = 38/70 (54.29%), Postives = 48/70 (68.57%), Query Frame = 1

Query:  178 DKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLD 387
            DK+ DQ+SDAVLDA L QD  ++VACET  KT + +V GEITT A +D E + R     IG+ S+DVG D
Sbjct:   17 DKVADQISDAVLDAILTQDQRARVACETMVKTGVAIVAGEITTSAWIDLEALTRKVITDIGYDSSDVGFD 86

HSP 2 Score: 28.1054 bits (61), Expect = 5.518e-17
Identity = 11/15 (73.33%), Postives = 14/15 (93.33%), Query Frame = 3

Query:  129 METFLFTSESVNEGH 173
            M ++LFTSESV+EGH
Sbjct:    1 MSSYLFTSESVSEGH 15

[Close]

BLAST of CF504285 vs. ExPASy Swiss-Prot
Match: METK_LACJO (S-adenosylmethionine synthetase OS=Lactobacillus johnsonii GN=metK PE=3 SV=1)

HSP 1 Score: 81.6481 bits (200), Expect = 5.519e-17
Identity = 37/70 (52.86%), Postives = 50/70 (71.43%), Query Frame = 1

Query:  178 DKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLD 387
            DK+ DQ+SDA+LDA LA+DPD +VACET   T +V+V GEI+T A VD + +VR T   IG+   ++G D
Sbjct:   19 DKVADQISDAILDAILAKDPDGRVACETTVTTGLVLVVGEISTSAYVDIQSVVRKTILEIGYNRPELGFD 88

HSP 2 Score: 25.0238 bits (53), Expect = 5.519e-17
Identity = 11/14 (78.57%), Postives = 12/14 (85.71%), Query Frame = 3

Query:  132 ETFLFTSESVNEGH 173
            E  LFTSESV+EGH
Sbjct:    4 EKRLFTSESVSEGH 17

[Close]

BLAST of CF504285 vs. ExPASy Swiss-Prot
Match: METK_ACIF5 (S-adenosylmethionine synthetase OS=Acidithiobacillus ferrooxidans (strain ATCC 53993) GN=metK PE=3 SV=1)

HSP 1 Score: 81.6481 bits (200), Expect = 5.523e-17
Identity = 38/70 (54.29%), Postives = 50/70 (71.43%), Query Frame = 1

Query:  178 DKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLD 387
            DK+ DQ+SDA+LDA LAQDP  +VA ET   T ++++ GEITT A VDY  + R T R IG+ S+D+G D
Sbjct:   18 DKVADQISDAILDAILAQDPRGRVAAETLITTGLIVLAGEITTTAVVDYADVARQTVRRIGYNSSDMGFD 87

HSP 2 Score: 25.0238 bits (53), Expect = 5.523e-17
Identity = 10/14 (71.43%), Postives = 13/14 (92.86%), Query Frame = 3

Query:  132 ETFLFTSESVNEGH 173
            ++ LFTSESV+EGH
Sbjct:    3 KSHLFTSESVSEGH 16

[Close]

BLAST of CF504285 vs. ExPASy Swiss-Prot
Match: METK_ACIF2 (S-adenosylmethionine synthetase OS=Acidithiobacillus ferrooxidans (strain ATCC 23270 / DSM 14882 / NCIB 8455) GN=metK PE=3 SV=1)

HSP 1 Score: 81.6481 bits (200), Expect = 5.523e-17
Identity = 38/70 (54.29%), Postives = 50/70 (71.43%), Query Frame = 1

Query:  178 DKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLD 387
            DK+ DQ+SDA+LDA LAQDP  +VA ET   T ++++ GEITT A VDY  + R T R IG+ S+D+G D
Sbjct:   18 DKVADQISDAILDAILAQDPRGRVAAETLITTGLIVLAGEITTTAVVDYADVARQTVRRIGYNSSDMGFD 87

HSP 2 Score: 25.0238 bits (53), Expect = 5.523e-17
Identity = 10/14 (71.43%), Postives = 13/14 (92.86%), Query Frame = 3

Query:  132 ETFLFTSESVNEGH 173
            ++ LFTSESV+EGH
Sbjct:    3 KSHLFTSESVSEGH 16

The following BLAST results are available for this feature:

BLAST of CF504285 vs. ExPASy Swiss-Prot

BLAST of CF504285 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500


Match Name	E-value	Identity	Description
METK_ACTPJ	4.250e-17	56.34	S-adenosylmethionine synthetase OS=Actinobacillus ...	[more]
METK_ACTP7	4.250e-17	56.34	S-adenosylmethionine synthetase OS=Actinobacillus ...	[more]
METK_ACTP2	4.250e-17	56.34	S-adenosylmethionine synthetase OS=Actinobacillus ...	[more]
METK_DESMR	5.333e-17	48.28	S-adenosylmethionine synthetase OS=Desulfovibrio m...	[more]
METK_BIFLI	5.333e-17	56.34	S-adenosylmethionine synthetase OS=Bifidobacterium...	[more]
METK_BACSK	5.333e-17	46.07	S-adenosylmethionine synthetase OS=Bacillus clausi...	[more]
METK_STRMK	5.518e-17	54.29	S-adenosylmethionine synthetase OS=Stenotrophomona...	[more]
METK_LACJO	5.519e-17	52.86	S-adenosylmethionine synthetase OS=Lactobacillus j...	[more]
METK_ACIF5	5.523e-17	54.29	S-adenosylmethionine synthetase OS=Acidithiobacill...	[more]
METK_ACIF2	5.523e-17	54.29	S-adenosylmethionine synthetase OS=Acidithiobacill...	[more]

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Properties


Property Name	Value
Genbank description	USDA-FP_119000-774 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-16_E09 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CF504285 ID=CF504285; Name=CF504285; organism=Citrus sinensis; type=EST; length=390bp
CGTCTTTCTCTCGTTCTTCTGGGTACTTACCATTTTCGTATATCACCACC
TTCAATTCCATTTGTACTCGCACCACGGCCTCCTGCAAAGCGAAAGCTGC
AGCTTCAGGTTAAATCAAATTAAAGAAAATGGAAACATTTCTTTTCACAT
CAGAATCAGTGAACGAGGGTCACCTCGGACAAGCTGTGTGACCAAGTTTC
GGACGCAGTACTGGACGCGTGTTTGGCTCAGGATCCTGACAGCAAAGTTG
CTTGTGAAACATGCACAAAGACCAACATGGTCATGGTTTTTGGAGAAATT
ACAACCAAGGCCAATGTGGATTACGAGAAAATCGTGCGTGATACATGCAG
ATCGATTGGGTTCACATCGGCCGATGTGGGGCTTGATGCT