CF504285 | Citrus Genome Database

Overview


Name	CF504285
Unique Name	CF504285
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	390

Libraries


Library Name	Type
Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of CF504285 vs. ExPASy Swiss-Prot
Match: METK_BIFAA (S-adenosylmethionine synthetase OS=Bifidobacterium adolescentis (strain ATCC 15703 / DSM 20083) GN=metK PE=3 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 1.552e-16
Identity = 40/71 (56.34%), Postives = 50/71 (70.42%), Query Frame = 1

Query:  178 DKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLDA 390
            DK+CDQ+SDA+LD  LAQDP S VA ETC       VFGE+T++   D + IVR   R+IG+TS+ VGLDA
Sbjct:   18 DKVCDQISDAILDDMLAQDPHSHVAVETCATVGQFFVFGEVTSEGYSDIQNIVRSVVRNIGYTSSRVGLDA 88

[Close]

BLAST of CF504285 vs. ExPASy Swiss-Prot
Match: METK_PROM4 (S-adenosylmethionine synthetase OS=Prochlorococcus marinus (strain MIT 9211) GN=metK PE=3 SV=1)

HSP 1 Score: 78.1814 bits (191), Expect = 1.575e-16
Identity = 34/68 (50.00%), Postives = 46/68 (67.65%), Query Frame = 1

Query:  178 DKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRSIGFTSADVG 381
            DK+CDQ+SDAVLDA L QDP S+VACE    T + ++ GE+T+ A VD+  +VR   + IG+  A  G
Sbjct:   17 DKICDQISDAVLDALLTQDPHSRVACEAVVNTGLCLITGEVTSTAEVDFINLVRSVIKEIGYKDAQAG 84

HSP 2 Score: 26.9498 bits (58), Expect = 1.575e-16
Identity = 11/15 (73.33%), Postives = 12/15 (80.00%), Query Frame = 3

Query:  129 METFLFTSESVNEGH 173
            M  F+FTSESV EGH
Sbjct:    1 MSQFVFTSESVTEGH 15

[Close]

BLAST of CF504285 vs. ExPASy Swiss-Prot
Match: METK_STRMU (S-adenosylmethionine synthetase OS=Streptococcus mutans GN=metK PE=3 SV=1)

HSP 1 Score: 80.1073 bits (196), Expect = 1.576e-16
Identity = 39/71 (54.93%), Postives = 47/71 (66.20%), Query Frame = 1

Query:  178 DKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLDA 390
            DK+ DQ+SDA+LDA L QDPD+ VA ET   T  V VFGEI+T A VD  ++VRDT   IG+T    G  A
Sbjct:   18 DKIADQISDAILDAVLVQDPDAHVAAETAVYTGSVHVFGEISTTAYVDINRVVRDTIAEIGYTDTAYGFSA 88

HSP 2 Score: 25.0238 bits (53), Expect = 1.576e-16
Identity = 11/14 (78.57%), Postives = 12/14 (85.71%), Query Frame = 3

Query:  132 ETFLFTSESVNEGH 173
            E  LFTSESV+EGH
Sbjct:    3 ERKLFTSESVSEGH 16

[Close]

BLAST of CF504285 vs. ExPASy Swiss-Prot
Match: METK_AKKM8 (S-adenosylmethionine synthetase OS=Akkermansia muciniphila (strain ATCC BAA-835) GN=metK PE=3 SV=1)

HSP 1 Score: 81.6481 bits (200), Expect = 1.576e-16
Identity = 37/62 (59.68%), Postives = 48/62 (77.42%), Query Frame = 1

Query:  178 DKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRSIGF 363
            DK+ D +SD++LDACLAQD  S+VACET  K+NMV++ GE+TTKA +D EKI R   R IG+
Sbjct:   20 DKVADYISDSILDACLAQDKTSRVACETLVKSNMVIIAGELTTKAVIDPEKIARQAIREIGY 81

HSP 2 Score: 23.483 bits (49), Expect = 1.576e-16
Identity = 9/11 (81.82%), Postives = 10/11 (90.91%), Query Frame = 3

Query:  141 LFTSESVNEGH 173
            +FTSESV EGH
Sbjct:    8 IFTSESVGEGH 18

[Close]

BLAST of CF504285 vs. ExPASy Swiss-Prot
Match: METK_PSEPK (S-adenosylmethionine synthetase OS=Pseudomonas putida (strain KT2440) GN=metK PE=3 SV=1)

HSP 1 Score: 80.1073 bits (196), Expect = 1.576e-16
Identity = 38/70 (54.29%), Postives = 50/70 (71.43%), Query Frame = 1

Query:  178 DKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLD 387
            DK+ DQ+SDAVLDA +AQD  ++VACET  KT + ++ GE+TT A VD E +VR     IG+ S+DVG D
Sbjct:   18 DKIADQISDAVLDAIIAQDKYARVACETLVKTGVAIIAGEVTTSAWVDLEDLVRKVIIDIGYNSSDVGFD 87

HSP 2 Score: 25.0238 bits (53), Expect = 1.576e-16
Identity = 11/14 (78.57%), Postives = 12/14 (85.71%), Query Frame = 3

Query:  132 ETFLFTSESVNEGH 173
            E  LFTSESV+EGH
Sbjct:    3 EYSLFTSESVSEGH 16

[Close]

BLAST of CF504285 vs. ExPASy Swiss-Prot
Match: METK_PSEPG (S-adenosylmethionine synthetase OS=Pseudomonas putida (strain GB-1) GN=metK PE=3 SV=1)

HSP 1 Score: 80.1073 bits (196), Expect = 1.576e-16
Identity = 38/70 (54.29%), Postives = 50/70 (71.43%), Query Frame = 1

Query:  178 DKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLD 387
            DK+ DQ+SDAVLDA +AQD  ++VACET  KT + ++ GE+TT A VD E +VR     IG+ S+DVG D
Sbjct:   18 DKIADQISDAVLDAIIAQDKYARVACETLVKTGVAIIAGEVTTSAWVDLEDLVRKVIIDIGYNSSDVGFD 87

HSP 2 Score: 25.0238 bits (53), Expect = 1.576e-16
Identity = 11/14 (78.57%), Postives = 12/14 (85.71%), Query Frame = 3

Query:  132 ETFLFTSESVNEGH 173
            E  LFTSESV+EGH
Sbjct:    3 EYSLFTSESVSEGH 16

[Close]

BLAST of CF504285 vs. ExPASy Swiss-Prot
Match: METK_PSEP1 (S-adenosylmethionine synthetase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=metK PE=3 SV=1)

HSP 1 Score: 80.1073 bits (196), Expect = 1.576e-16
Identity = 38/70 (54.29%), Postives = 50/70 (71.43%), Query Frame = 1

Query:  178 DKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLD 387
            DK+ DQ+SDAVLDA +AQD  ++VACET  KT + ++ GE+TT A VD E +VR     IG+ S+DVG D
Sbjct:   18 DKIADQISDAVLDAIIAQDKYARVACETLVKTGVAIIAGEVTTSAWVDLEDLVRKVIIDIGYNSSDVGFD 87

HSP 2 Score: 25.0238 bits (53), Expect = 1.576e-16
Identity = 11/14 (78.57%), Postives = 12/14 (85.71%), Query Frame = 3

Query:  132 ETFLFTSESVNEGH 173
            E  LFTSESV+EGH
Sbjct:    3 EYSLFTSESVSEGH 16

[Close]

BLAST of CF504285 vs. ExPASy Swiss-Prot
Match: METK_AZOVD (S-adenosylmethionine synthetase OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=metK PE=3 SV=1)

HSP 1 Score: 80.1073 bits (196), Expect = 1.576e-16
Identity = 38/70 (54.29%), Postives = 51/70 (72.86%), Query Frame = 1

Query:  178 DKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLD 387
            DK+ DQ+SDAVLDA +A+D  ++VACET  KT + +V GEITT A +D E++VR     IG+ S+DVG D
Sbjct:   18 DKIADQISDAVLDAIIAEDKHARVACETLVKTGVAIVAGEITTSAWIDLEELVRKVIVDIGYDSSDVGYD 87

HSP 2 Score: 25.0238 bits (53), Expect = 1.576e-16
Identity = 11/14 (78.57%), Postives = 12/14 (85.71%), Query Frame = 3

Query:  132 ETFLFTSESVNEGH 173
            E  LFTSESV+EGH
Sbjct:    3 EYSLFTSESVSEGH 16

[Close]

BLAST of CF504285 vs. ExPASy Swiss-Prot
Match: METK_PEDPA (S-adenosylmethionine synthetase OS=Pediococcus pentosaceus (strain ATCC 25745 / 183-1w) GN=metK PE=3 SV=1)

HSP 1 Score: 79.337 bits (194), Expect = 1.576e-16
Identity = 34/70 (48.57%), Postives = 49/70 (70.00%), Query Frame = 1

Query:  178 DKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLD 387
            DK+ DQ+SD++LD  L  DPD++VACET   T +V+V GEI+T A +D +K+VR T + IG+   + G D
Sbjct:   18 DKIADQISDSILDELLKNDPDARVACETTVTTGLVVVVGEISTTAYIDIQKVVRKTIKDIGYVGGEYGFD 87

HSP 2 Score: 25.7942 bits (55), Expect = 1.576e-16
Identity = 11/14 (78.57%), Postives = 12/14 (85.71%), Query Frame = 3

Query:  132 ETFLFTSESVNEGH 173
            E  LFTSESV+EGH
Sbjct:    3 ERHLFTSESVSEGH 16

[Close]

BLAST of CF504285 vs. ExPASy Swiss-Prot
Match: METK_RALPJ (S-adenosylmethionine synthetase OS=Ralstonia pickettii (strain 12J) GN=metK PE=3 SV=1)

HSP 1 Score: 78.1814 bits (191), Expect = 1.576e-16
Identity = 36/70 (51.43%), Postives = 51/70 (72.86%), Query Frame = 1

Query:  178 DKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLD 387
            DK+ DQ+SDA+LDA LAQD  ++VA ET   T +V++ GEITT ANVDY  + R+T + IG+ + + G+D
Sbjct:   18 DKVADQISDAILDAILAQDKYARVAAETLCNTGLVVLAGEITTTANVDYIHVARETIKRIGYDNTEYGID 87

HSP 2 Score: 26.9498 bits (58), Expect = 1.576e-16
Identity = 11/12 (91.67%), Postives = 12/12 (100.00%), Query Frame = 3

Query:  138 FLFTSESVNEGH 173
            FLFTSESV+EGH
Sbjct:    5 FLFTSESVSEGH 16

The following BLAST results are available for this feature:

BLAST of CF504285 vs. ExPASy Swiss-Prot

BLAST of CF504285 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500


Match Name	E-value	Identity	Description
METK_BIFAA	1.552e-16	56.34	S-adenosylmethionine synthetase OS=Bifidobacterium...	[more]
METK_PROM4	1.575e-16	50.00	S-adenosylmethionine synthetase OS=Prochlorococcus...	[more]
METK_STRMU	1.576e-16	54.93	S-adenosylmethionine synthetase OS=Streptococcus m...	[more]
METK_AKKM8	1.576e-16	59.68	S-adenosylmethionine synthetase OS=Akkermansia muc...	[more]
METK_PSEPK	1.576e-16	54.29	S-adenosylmethionine synthetase OS=Pseudomonas put...	[more]
METK_PSEPG	1.576e-16	54.29	S-adenosylmethionine synthetase OS=Pseudomonas put...	[more]
METK_PSEP1	1.576e-16	54.29	S-adenosylmethionine synthetase OS=Pseudomonas put...	[more]
METK_AZOVD	1.576e-16	54.29	S-adenosylmethionine synthetase OS=Azotobacter vin...	[more]
METK_PEDPA	1.576e-16	48.57	S-adenosylmethionine synthetase OS=Pediococcus pen...	[more]
METK_RALPJ	1.576e-16	51.43	S-adenosylmethionine synthetase OS=Ralstonia picke...	[more]

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Properties


Property Name	Value
Genbank description	USDA-FP_119000-774 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-16_E09 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CF504285 ID=CF504285; Name=CF504285; organism=Citrus sinensis; type=EST; length=390bp
CGTCTTTCTCTCGTTCTTCTGGGTACTTACCATTTTCGTATATCACCACC
TTCAATTCCATTTGTACTCGCACCACGGCCTCCTGCAAAGCGAAAGCTGC
AGCTTCAGGTTAAATCAAATTAAAGAAAATGGAAACATTTCTTTTCACAT
CAGAATCAGTGAACGAGGGTCACCTCGGACAAGCTGTGTGACCAAGTTTC
GGACGCAGTACTGGACGCGTGTTTGGCTCAGGATCCTGACAGCAAAGTTG
CTTGTGAAACATGCACAAAGACCAACATGGTCATGGTTTTTGGAGAAATT
ACAACCAAGGCCAATGTGGATTACGAGAAAATCGTGCGTGATACATGCAG
ATCGATTGGGTTCACATCGGCCGATGTGGGGCTTGATGCT