CF504285

Overview
NameCF504285
Unique NameCF504285
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length390
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig12594 contig Csv1_Contig12594:72..460. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CF504285 vs. ExPASy Swiss-Prot
Match: METK_ANADE (S-adenosylmethionine synthetase OS=Anaeromyxobacter dehalogenans (strain 2CP-C) GN=metK PE=3 SV=1)

HSP 1 Score: 97.0561 bits (240), Expect = 2.389e-22
Identity = 45/71 (63.38%), Postives = 54/71 (76.06%), Query Frame = 1 
Query:  178 DKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLDA 390
            DK+ DQ+SDAVLDA L QDP  +VACET  KT  VM+ GEITTKA +DY K+ R+T R IG+TS D+G DA
Sbjct:   19 DKMADQISDAVLDAVLRQDPKGRVACETLLKTGYVMIAGEITTKARIDYPKLARETVRRIGYTSGDMGFDA 89

HSP 2 Score: 27.7202 bits (60), Expect = 2.389e-22
Identity = 11/15 (73.33%), Postives = 13/15 (86.67%), Query Frame = 3 
Query:  129 METFLFTSESVNEGH 173
            ++ FLFTSESV EGH
Sbjct:    3 LQDFLFTSESVTEGH 17
[Close]
BLAST of CF504285 vs. ExPASy Swiss-Prot
Match: METK_ANAD2 (S-adenosylmethionine synthetase OS=Anaeromyxobacter dehalogenans (strain 2CP-1 / ATCC BAA-258) GN=metK PE=3 SV=1)

HSP 1 Score: 97.0561 bits (240), Expect = 2.389e-22
Identity = 45/71 (63.38%), Postives = 54/71 (76.06%), Query Frame = 1 
Query:  178 DKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLDA 390
            DK+ DQ+SDAVLDA L QDP  +VACET  KT  VM+ GEITTKA +DY K+ R+T R IG+TS D+G DA
Sbjct:   19 DKMADQISDAVLDAVLRQDPKGRVACETLLKTGYVMIAGEITTKARIDYPKLARETVRRIGYTSGDMGFDA 89

HSP 2 Score: 27.7202 bits (60), Expect = 2.389e-22
Identity = 11/15 (73.33%), Postives = 13/15 (86.67%), Query Frame = 3 
Query:  129 METFLFTSESVNEGH 173
            ++ FLFTSESV EGH
Sbjct:    3 LQDFLFTSESVTEGH 17
[Close]
BLAST of CF504285 vs. ExPASy Swiss-Prot
Match: METK_PROMH (S-adenosylmethionine synthetase OS=Proteus mirabilis (strain HI4320) GN=metK PE=3 SV=1)

HSP 1 Score: 96.6709 bits (239), Expect = 3.109e-22
Identity = 48/71 (67.61%), Postives = 56/71 (78.87%), Query Frame = 1 
Query:  178 DKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLDA 390
            DK+ DQ+SDAVLDA L QDP ++VACET  KT MVMV GEITTKA VD E+I R T R IG+TS+D+G DA
Sbjct:   17 DKIADQISDAVLDAILEQDPKARVACETYVKTGMVMVGGEITTKAWVDIEEITRKTVREIGYTSSDMGFDA 87

HSP 2 Score: 27.7202 bits (60), Expect = 3.109e-22
Identity = 12/15 (80.00%), Postives = 13/15 (86.67%), Query Frame = 3 
Query:  129 METFLFTSESVNEGH 173
            M T LFTSESV+EGH
Sbjct:    1 MTTHLFTSESVSEGH 15
[Close]
BLAST of CF504285 vs. ExPASy Swiss-Prot
Match: METK1_CAEEL (Probable S-adenosylmethionine synthetase 1 OS=Caenorhabditis elegans GN=sams-1 PE=2 SV=1)

HSP 1 Score: 95.5153 bits (236), Expect = 1.158e-21
Identity = 45/70 (64.29%), Postives = 55/70 (78.57%), Query Frame = 1 
Query:  178 DKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLD 387
            DK+CDQ+SDAVLDA L QDP++KVACET TKT MVM+ GEIT+KA VDY+ +VR     IGFT + +G D
Sbjct:   18 DKMCDQISDAVLDAHLKQDPNAKVACETVTKTGMVMLCGEITSKAVVDYQVLVRRVIEKIGFTDSSIGFD 87

HSP 2 Score: 26.9498 bits (58), Expect = 1.158e-21
Identity = 11/12 (91.67%), Postives = 12/12 (100.00%), Query Frame = 3 
Query:  138 FLFTSESVNEGH 173
            FLFTSESV+EGH
Sbjct:    5 FLFTSESVSEGH 16
[Close]
BLAST of CF504285 vs. ExPASy Swiss-Prot
Match: METK_TREPS (S-adenosylmethionine synthetase OS=Treponema pallidum subsp. pallidum (strain SS14) GN=metK PE=3 SV=1)

HSP 1 Score: 95.5153 bits (236), Expect = 1.507e-21
Identity = 43/71 (60.56%), Postives = 57/71 (80.28%), Query Frame = 1 
Query:  178 DKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLDA 390
            DKLCDQ+SDAVLDACL+QDP S VACET   T+++++ GEI+T+A+++  +I RD    IG+ SADVGLDA
Sbjct:   16 DKLCDQISDAVLDACLSQDPHSCVACETFASTSLILIGGEISTRAHINLTQIARDVAADIGYVSADVGLDA 86

HSP 2 Score: 26.5646 bits (57), Expect = 1.507e-21
Identity = 13/15 (86.67%), Postives = 14/15 (93.33%), Query Frame = 3 
Query:  129 METFLFTSESVNEGH 173
            METF FTSESV+EGH
Sbjct:    1 METF-FTSESVSEGH 14
[Close]
BLAST of CF504285 vs. ExPASy Swiss-Prot
Match: METK_TREPA (S-adenosylmethionine synthetase OS=Treponema pallidum GN=metK PE=3 SV=1)

HSP 1 Score: 95.5153 bits (236), Expect = 1.507e-21
Identity = 43/71 (60.56%), Postives = 57/71 (80.28%), Query Frame = 1 
Query:  178 DKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLDA 390
            DKLCDQ+SDAVLDACL+QDP S VACET   T+++++ GEI+T+A+++  +I RD    IG+ SADVGLDA
Sbjct:   16 DKLCDQISDAVLDACLSQDPHSCVACETFASTSLILIGGEISTRAHINLTQIARDVAADIGYVSADVGLDA 86

HSP 2 Score: 26.5646 bits (57), Expect = 1.507e-21
Identity = 13/15 (86.67%), Postives = 14/15 (93.33%), Query Frame = 3 
Query:  129 METFLFTSESVNEGH 173
            METF FTSESV+EGH
Sbjct:    1 METF-FTSESVSEGH 14
[Close]
BLAST of CF504285 vs. ExPASy Swiss-Prot
Match: METK_DROME (S-adenosylmethionine synthetase OS=Drosophila melanogaster GN=M(2)21AB PE=1 SV=2)

HSP 1 Score: 92.8189 bits (229), Expect = 2.548e-21
Identity = 41/70 (58.57%), Postives = 56/70 (80.00%), Query Frame = 1 
Query:  178 DKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLD 387
            DK+CDQ+SDA+LDA L QDP++KVACET  KT M+++ GEIT+KA VDY+K+VR+T + IG+  +  G D
Sbjct:   42 DKMCDQISDAILDAHLKQDPNAKVACETVAKTGMILLCGEITSKAVVDYQKVVRETVQHIGYDDSSKGFD 111

HSP 2 Score: 28.4906 bits (62), Expect = 2.548e-21
Identity = 12/13 (92.31%), Postives = 12/13 (92.31%), Query Frame = 3 
Query:  135 TFLFTSESVNEGH 173
            TFLFTSESV EGH
Sbjct:   28 TFLFTSESVGEGH 40
[Close]
BLAST of CF504285 vs. ExPASy Swiss-Prot
Match: METK2_PONAB (S-adenosylmethionine synthetase isoform type-2 OS=Pongo abelii GN=MAT2A PE=2 SV=1)

HSP 1 Score: 92.8189 bits (229), Expect = 2.550e-21
Identity = 41/70 (58.57%), Postives = 55/70 (78.57%), Query Frame = 1 
Query:  178 DKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLD 387
            DK+CDQ+SDAVLDA L QDPD+KVACET  KT M+++ GEIT++A VDY+K+VR+  + IG+  +  G D
Sbjct:   31 DKICDQISDAVLDAHLQQDPDAKVACETVAKTGMILLAGEITSRAAVDYQKVVREAVKHIGYDDSSKGFD 100

HSP 2 Score: 28.4906 bits (62), Expect = 2.550e-21
Identity = 12/13 (92.31%), Postives = 12/13 (92.31%), Query Frame = 3 
Query:  135 TFLFTSESVNEGH 173
            TFLFTSESV EGH
Sbjct:   17 TFLFTSESVGEGH 29
[Close]
BLAST of CF504285 vs. ExPASy Swiss-Prot
Match: METK2_MACFA (S-adenosylmethionine synthetase isoform type-2 OS=Macaca fascicularis GN=MAT2A PE=2 SV=1)

HSP 1 Score: 92.8189 bits (229), Expect = 2.550e-21
Identity = 41/70 (58.57%), Postives = 55/70 (78.57%), Query Frame = 1 
Query:  178 DKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLD 387
            DK+CDQ+SDAVLDA L QDPD+KVACET  KT M+++ GEIT++A VDY+K+VR+  + IG+  +  G D
Sbjct:   31 DKICDQISDAVLDAHLQQDPDAKVACETVAKTGMILLAGEITSRAAVDYQKVVREAVKHIGYDDSSKGFD 100

HSP 2 Score: 28.4906 bits (62), Expect = 2.550e-21
Identity = 12/13 (92.31%), Postives = 12/13 (92.31%), Query Frame = 3 
Query:  135 TFLFTSESVNEGH 173
            TFLFTSESV EGH
Sbjct:   17 TFLFTSESVGEGH 29
[Close]
BLAST of CF504285 vs. ExPASy Swiss-Prot
Match: METK2_HUMAN (S-adenosylmethionine synthetase isoform type-2 OS=Homo sapiens GN=MAT2A PE=1 SV=1)

HSP 1 Score: 92.8189 bits (229), Expect = 2.550e-21
Identity = 41/70 (58.57%), Postives = 55/70 (78.57%), Query Frame = 1 
Query:  178 DKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLD 387
            DK+CDQ+SDAVLDA L QDPD+KVACET  KT M+++ GEIT++A VDY+K+VR+  + IG+  +  G D
Sbjct:   31 DKICDQISDAVLDAHLQQDPDAKVACETVAKTGMILLAGEITSRAAVDYQKVVREAVKHIGYDDSSKGFD 100

HSP 2 Score: 28.4906 bits (62), Expect = 2.550e-21
Identity = 12/13 (92.31%), Postives = 12/13 (92.31%), Query Frame = 3 
Query:  135 TFLFTSESVNEGH 173
            TFLFTSESV EGH
Sbjct:   17 TFLFTSESVGEGH 29
The following BLAST results are available for this feature:
BLAST of CF504285 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
METK_ANADE2.389e-2263.38S-adenosylmethionine synthetase OS=Anaeromyxobacte... [more]
METK_ANAD22.389e-2263.38S-adenosylmethionine synthetase OS=Anaeromyxobacte... [more]
METK_PROMH3.109e-2267.61S-adenosylmethionine synthetase OS=Proteus mirabil... [more]
METK1_CAEEL1.158e-2164.29Probable S-adenosylmethionine synthetase 1 OS=Caen... [more]
METK_TREPS1.507e-2160.56S-adenosylmethionine synthetase OS=Treponema palli... [more]
METK_TREPA1.507e-2160.56S-adenosylmethionine synthetase OS=Treponema palli... [more]
METK_DROME2.548e-2158.57S-adenosylmethionine synthetase OS=Drosophila mela... [more]
METK2_PONAB2.550e-2158.57S-adenosylmethionine synthetase isoform type-2 OS=... [more]
METK2_MACFA2.550e-2158.57S-adenosylmethionine synthetase isoform type-2 OS=... [more]
METK2_HUMAN2.550e-2158.57S-adenosylmethionine synthetase isoform type-2 OS=... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_119000-774 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-16_E09 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF504285 ID=CF504285; Name=CF504285; organism=Citrus sinensis; type=EST; length=390bp
CGTCTTTCTCTCGTTCTTCTGGGTACTTACCATTTTCGTATATCACCACC
TTCAATTCCATTTGTACTCGCACCACGGCCTCCTGCAAAGCGAAAGCTGC
AGCTTCAGGTTAAATCAAATTAAAGAAAATGGAAACATTTCTTTTCACAT
CAGAATCAGTGAACGAGGGTCACCTCGGACAAGCTGTGTGACCAAGTTTC
GGACGCAGTACTGGACGCGTGTTTGGCTCAGGATCCTGACAGCAAAGTTG
CTTGTGAAACATGCACAAAGACCAACATGGTCATGGTTTTTGGAGAAATT
ACAACCAAGGCCAATGTGGATTACGAGAAAATCGTGCGTGATACATGCAG
ATCGATTGGGTTCACATCGGCCGATGTGGGGCTTGATGCT
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