CF504368

Overview
NameCF504368
Unique NameCF504368
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length336
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig7217 contig Csv1_Contig7217:969..1302. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CF504368 vs. ExPASy Swiss-Prot
Match: IREB2_MOUSE (Iron-responsive element-binding protein 2 OS=Mus musculus GN=Ireb2 PE=2 SV=1)

HSP 1 Score: 82.0333 bits (201), Expect = 1.460e-17
Identity = 37/80 (46.25%), Postives = 50/80 (62.50%), Query Frame = 3 
Query:    6 VLPDMFKSTYEAITKGNPTWNQLSVPASKLYSWDPNSTYIHEPPYFKDMTMDPPGAHGVKDAYCLLNFGDSITTDHISPA 245
            V+  MFK+  E +  GN  WN L  P S L+ WD  STYI  P +F  +T +P  +  +++A+ LL  GDS+TTDHISPA
Sbjct:  679 VILSMFKTLKEKVEMGNKRWNSLEAPDSVLFPWDVKSTYIRCPSFFDKLTKEPAASQPIENAHVLLYLGDSVTTDHISPA 758

HSP 2 Score: 26.5646 bits (57), Expect = 1.460e-17
Identity = 12/33 (36.36%), Postives = 17/33 (51.52%), Query Frame = 2 
Query:  233 HFSGEEVSTKTVPLQSYLLERGVERRDFNSYGS 331
            H S      ++     YL  RG+  R+FNSYG+
Sbjct:  754 HISPAGSIARSSAAAKYLTNRGLTPREFNSYGA 786
[Close]
BLAST of CF504368 vs. ExPASy Swiss-Prot
Match: ACON_CORGL (Aconitate hydratase OS=Corynebacterium glutamicum GN=acn PE=1 SV=2)

HSP 1 Score: 77.7962 bits (190), Expect = 2.468e-17
Identity = 32/77 (41.56%), Postives = 47/77 (61.04%), Query Frame = 3 
Query:   15 DMFKSTYEAITKGNPTWNQLSVPASKLYSWDPNSTYIHEPPYFKDMTMDPPGAHGVKDAYCLLNFGDSITTDHISPA 245
            +++++ Y  + KG+  W +L VP    + WD NSTYI + PYF  M ++P     ++ A  L   GDS+TTDHISPA
Sbjct:  645 ELYEADYADVFKGDKQWQELDVPTGDTFEWDENSTYIRKAPYFDGMPVEPVAVTDIQGARVLAKLGDSVTTDHISPA 721

HSP 2 Score: 30.0314 bits (66), Expect = 2.468e-17
Identity = 15/33 (45.45%), Postives = 16/33 (48.48%), Query Frame = 2 
Query:  233 HFSGEEVSTKTVPLQSYLLERGVERRDFNSYGS 331
            H S         P   YL E GVER D+NS GS
Sbjct:  717 HISPASSIKPGTPAAQYLDEHGVERHDYNSLGS 749
[Close]
BLAST of CF504368 vs. ExPASy Swiss-Prot
Match: ACON_CORDI (Aconitate hydratase OS=Corynebacterium diphtheriae GN=acn PE=2 SV=1)

HSP 1 Score: 80.1073 bits (196), Expect = 2.468e-17
Identity = 34/77 (44.16%), Postives = 46/77 (59.74%), Query Frame = 3 
Query:   15 DMFKSTYEAITKGNPTWNQLSVPASKLYSWDPNSTYIHEPPYFKDMTMDPPGAHGVKDAYCLLNFGDSITTDHISPA 245
            +++++ Y  + KG+  W  L  P  K + WD  STYI + PYF  MTM+P     +K A  L   GDS+TTDHISPA
Sbjct:  643 ELYEADYADVFKGDEQWQNLPTPEGKTFDWDEKSTYIRKAPYFDGMTMEPAPVSDIKGARVLAKLGDSVTTDHISPA 719

HSP 2 Score: 27.7202 bits (60), Expect = 2.468e-17
Identity = 14/33 (42.42%), Postives = 15/33 (45.45%), Query Frame = 2 
Query:  233 HFSGEEVSTKTVPLQSYLLERGVERRDFNSYGS 331
            H S         P   YL E GV R D+NS GS
Sbjct:  715 HISPASSIKPGTPAAQYLDENGVARNDYNSLGS 747
[Close]
BLAST of CF504368 vs. ExPASy Swiss-Prot
Match: ACON_DEIRA (Aconitate hydratase OS=Deinococcus radiodurans GN=acn PE=1 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 2.470e-17
Identity = 34/84 (40.48%), Postives = 46/84 (54.76%), Query Frame = 3 
Query:    3 SVLPDMFKSTYEAITKGNPTWNQLSVPASKLYSWDPNSTYIHEPPYFKDMTMDPPGAHGVKDAYCLLNFGDSITTDHISPARKY 254
            S+  +MFK  Y+ I K N  WN + V    L+ W  +STYI  PP+F  +         +K A  L+  GDS+TTDHISPA  +
Sbjct:  607 SINAEMFKKVYDGIEKSNADWNAIPVAEGALFDWKEDSTYIQNPPFFDTLAGGAHEIESIKGARALVKVGDSVTTDHISPAGSF 690

HSP 2 Score: 31.5722 bits (70), Expect = 2.470e-17
Identity = 13/21 (61.90%), Postives = 15/21 (71.43%), Query Frame = 2 
Query:  269 PLQSYLLERGVERRDFNSYGS 331
            P   YL ERG+  +DFNSYGS
Sbjct:  695 PAGRYLTERGIAPKDFNSYGS 715
[Close]
BLAST of CF504368 vs. ExPASy Swiss-Prot
Match: IREB2_PIG (Iron-responsive element-binding protein 2 OS=Sus scrofa GN=IREB2 PE=2 SV=1)

HSP 1 Score: 80.4925 bits (197), Expect = 4.169e-17
Identity = 38/80 (47.50%), Postives = 49/80 (61.25%), Query Frame = 3 
Query:    6 VLPDMFKSTYEAITKGNPTWNQLSVPASKLYSWDPNSTYIHEPPYFKDMTMDPPGAHGVKDAYCLLNFGDSITTDHISPA 245
            V+  MFK+  E I  GN  WN L  P S L+ WD  STYI  P +F  +T +P     +++A+ LL  GDS+TTDHISPA
Sbjct:  680 VVLSMFKALKEKIEMGNKRWNSLEAPDSVLFPWDLKSTYIRCPSFFDKLTKEPVALQPIENAHVLLYLGDSVTTDHISPA 759

HSP 2 Score: 26.5646 bits (57), Expect = 4.169e-17
Identity = 12/33 (36.36%), Postives = 17/33 (51.52%), Query Frame = 2 
Query:  233 HFSGEEVSTKTVPLQSYLLERGVERRDFNSYGS 331
            H S      ++     YL  RG+  R+FNSYG+
Sbjct:  755 HISPAGSIARSSAAAKYLTNRGLTPREFNSYGA 787
[Close]
BLAST of CF504368 vs. ExPASy Swiss-Prot
Match: IREB2_CHICK (Iron-responsive element-binding protein 2 OS=Gallus gallus GN=IREB2 PE=2 SV=1)

HSP 1 Score: 81.2629 bits (199), Expect = 5.419e-17
Identity = 37/80 (46.25%), Postives = 48/80 (60.00%), Query Frame = 3 
Query:    6 VLPDMFKSTYEAITKGNPTWNQLSVPASKLYSWDPNSTYIHEPPYFKDMTMDPPGAHGVKDAYCLLNFGDSITTDHISPA 245
            V+  MFK   E + KGN  WN L  P S L+ WD  STYI  P +F  +  +P     +++A+ LL  GDS+TTDHISPA
Sbjct:  681 VISSMFKELKEKMEKGNKRWNSLEAPESPLFPWDLKSTYIRCPSFFDKLAKEPVSLQPIENAHVLLYLGDSVTTDHISPA 760

HSP 2 Score: 25.409 bits (54), Expect = 5.419e-17
Identity = 11/33 (33.33%), Postives = 17/33 (51.52%), Query Frame = 2 
Query:  233 HFSGEEVSTKTVPLQSYLLERGVERRDFNSYGS 331
            H S      ++     YL  +G+  R+FNSYG+
Sbjct:  756 HISPAGSIARSSAAAKYLTNKGLTPREFNSYGA 788
[Close]
BLAST of CF504368 vs. ExPASy Swiss-Prot
Match: IREB2_HUMAN (Iron-responsive element-binding protein 2 OS=Homo sapiens GN=IREB2 PE=1 SV=2)

HSP 1 Score: 80.4925 bits (197), Expect = 5.419e-17
Identity = 38/91 (41.76%), Postives = 52/91 (57.14%), Query Frame = 3 
Query:    6 VLPDMFKSTYEAITKGNPTWNQLSVPASKLYSWDPNSTYIHEPPYFKDMTMDPPGAHGVKDAYCLLNFGDSITTDHISPARKYPQRQSHCK 278
            V+  MFK+  + I  GN  WN L  P S L+ WD  STYI  P +F  +T +P     +++A+ LL  GDS+TTDHISPA    +  +  K
Sbjct:  679 VILSMFKALKDKIEMGNKRWNSLEAPDSVLFPWDLKSTYIRCPSFFDKLTKEPIALQAIENAHVLLYLGDSVTTDHISPAGSIARNSAAAK 769

HSP 2 Score: 26.1794 bits (56), Expect = 5.419e-17
Identity = 10/17 (58.82%), Postives = 13/17 (76.47%), Query Frame = 2 
Query:  281 YLLERGVERRDFNSYGS 331
            YL  RG+  R+FNSYG+
Sbjct:  770 YLTNRGLTPREFNSYGA 786
[Close]
BLAST of CF504368 vs. ExPASy Swiss-Prot
Match: ACON1_PSEAE (Aconitate hydratase 1 OS=Pseudomonas aeruginosa GN=acnA PE=3 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 7.051e-17
Identity = 33/77 (42.86%), Postives = 43/77 (55.84%), Query Frame = 3 
Query:   15 DMFKSTYEAITKGNPTWNQLSVPASKLYSWDPNSTYIHEPPYFKDMTMDPPGAHGVKDAYCLLNFGDSITTDHISPA 245
            +MF   Y  +  G+  W  + VP S  Y W  +STYI  PP+F+ +   PP    V+ A  L   GDS+TTDHISPA
Sbjct:  623 EMFHKEYAEVFAGDEKWQAIQVPQSDTYEWQADSTYIQHPPFFEHIAEAPPAIADVEQARVLAVLGDSVTTDHISPA 699

HSP 2 Score: 31.9574 bits (71), Expect = 7.051e-17
Identity = 14/21 (66.67%), Postives = 15/21 (71.43%), Query Frame = 2 
Query:  269 PLQSYLLERGVERRDFNSYGS 331
            P   YL E GVE +DFNSYGS
Sbjct:  707 PAGRYLREHGVEPKDFNSYGS 727
[Close]
BLAST of CF504368 vs. ExPASy Swiss-Prot
Match: ACON_STAES (Aconitate hydratase OS=Staphylococcus epidermidis (strain ATCC 12228) GN=acnA PE=3 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 7.053e-17
Identity = 33/80 (41.25%), Postives = 48/80 (60.00%), Query Frame = 3 
Query:    6 VLPDMFKSTYEAITKGNPTWNQLSVPASKLYSWDPNSTYIHEPPYFKDMTMDPPGAHGVKDAYCLLNFGDSITTDHISPA 245
            V P++F   Y+ +   N  WN++ V    LY +DPNSTYI  P +F+ ++ +P     +K    +  FGDS+TTDHISPA
Sbjct:  610 VTPELFLEEYKNVYHNNEMWNEIDVTDEPLYDFDPNSTYIQNPTFFQGLSKEPGKIEPLKSLRVMGKFGDSVTTDHISPA 689

HSP 2 Score: 30.0314 bits (66), Expect = 7.053e-17
Identity = 15/33 (45.45%), Postives = 17/33 (51.52%), Query Frame = 2 
Query:  233 HFSGEEVSTKTVPLQSYLLERGVERRDFNSYGS 331
            H S      K  P   YLL+  V  R+FNSYGS
Sbjct:  685 HISPAGAIGKDTPAGKYLLDHDVAIRNFNSYGS 717
[Close]
BLAST of CF504368 vs. ExPASy Swiss-Prot
Match: ACON_STAEQ (Aconitate hydratase OS=Staphylococcus epidermidis (strain ATCC 35984 / RP62A) GN=acnA PE=3 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 7.053e-17
Identity = 33/80 (41.25%), Postives = 48/80 (60.00%), Query Frame = 3 
Query:    6 VLPDMFKSTYEAITKGNPTWNQLSVPASKLYSWDPNSTYIHEPPYFKDMTMDPPGAHGVKDAYCLLNFGDSITTDHISPA 245
            V P++F   Y+ +   N  WN++ V    LY +DPNSTYI  P +F+ ++ +P     +K    +  FGDS+TTDHISPA
Sbjct:  610 VTPELFLEEYKNVYHNNEMWNEIDVTDEPLYDFDPNSTYIQNPTFFQGLSKEPGKIEPLKSLRVMGKFGDSVTTDHISPA 689

HSP 2 Score: 30.0314 bits (66), Expect = 7.053e-17
Identity = 15/33 (45.45%), Postives = 17/33 (51.52%), Query Frame = 2 
Query:  233 HFSGEEVSTKTVPLQSYLLERGVERRDFNSYGS 331
            H S      K  P   YLL+  V  R+FNSYGS
Sbjct:  685 HISPAGAIGKDTPAGKYLLDHDVAIRNFNSYGS 717
The following BLAST results are available for this feature:
BLAST of CF504368 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 48
Match NameE-valueIdentityDescription
IREB2_MOUSE1.460e-1746.25Iron-responsive element-binding protein 2 OS=Mus m... [more]
ACON_CORGL2.468e-1741.56Aconitate hydratase OS=Corynebacterium glutamicum ... [more]
ACON_CORDI2.468e-1744.16Aconitate hydratase OS=Corynebacterium diphtheriae... [more]
ACON_DEIRA2.470e-1740.48Aconitate hydratase OS=Deinococcus radiodurans GN=... [more]
IREB2_PIG4.169e-1747.50Iron-responsive element-binding protein 2 OS=Sus s... [more]
IREB2_CHICK5.419e-1746.25Iron-responsive element-binding protein 2 OS=Gallu... [more]
IREB2_HUMAN5.419e-1741.76Iron-responsive element-binding protein 2 OS=Homo ... [more]
ACON1_PSEAE7.051e-1742.86Aconitate hydratase 1 OS=Pseudomonas aeruginosa GN... [more]
ACON_STAES7.053e-1741.25Aconitate hydratase OS=Staphylococcus epidermidis ... [more]
ACON_STAEQ7.053e-1741.25Aconitate hydratase OS=Staphylococcus epidermidis ... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_119000-857 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-17_E07 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF504368 ID=CF504368; Name=CF504368; organism=Citrus sinensis; type=EST; length=336bp
CCAGTGTGTTGCCAGACATGTTCAAAAGTACCTATGAGGCTATCACAAAG
GGAAATCCCACGTGGAATCAGTTATCTGTACCAGCTAGTAAACTCTATTC
TTGGGACCCTAACTCAACCTACATTCATGAACCTCCTTACTTCAAGGACA
TGACTATGGATCCTCCTGGAGCCCATGGAGTTAAGGATGCCTACTGCTTG
CTGAACTTTGGTGACAGTATCACTACAGATCACATTTCTCCGGCGAGGAA
GTATCCACAAAGACAGTCCCACTGCAAAGTTACCTGCTAGAGCGTGGTGT
GGAACGCAGGGACTTCAATTCTTATGGTAGTCCGTC
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