CF504403 | Citrus Genome Database

Overview


Name	CF504403
Unique Name	CF504403
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	495

Libraries


Library Name	Type
Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of CF504403 vs. ExPASy Swiss-Prot
Match: MPK9_ORYSJ (Mitogen-activated protein kinase 9 OS=Oryza sativa subsp. japonica GN=MPK9 PE=3 SV=2)

HSP 1 Score: 67.0106 bits (162), Expect = 5.186e-20
Identity = 28/61 (45.90%), Postives = 46/61 (75.41%), Query Frame = 2

Query:  254 FDNHMDAKRTLREIKLLQHFDHENVIAVKDVVPPPLRREFTDVYIAAELMDTDLYQIIRSN 436
            F++  DA R LREIKLL+   H +++ +K ++ PP RR+F D+Y+  ELM++DL+Q+I++N
Sbjct:  142 FEHVSDAARILREIKLLRLLRHPDIVEIKHIMLPPSRRDFKDIYVVFELMESDLHQVIKAN 202

HSP 2 Score: 38.5058 bits (88), Expect = 5.186e-20
Identity = 15/30 (50.00%), Postives = 23/30 (76.67%), Query Frame = 1

Query:  166 IGRGAYGIVCSVLNTETNELVAMKKIANRF 255
            IG+G+YG+VCS ++  T + VA+KK+ N F
Sbjct:  113 IGKGSYGVVCSAIDLHTRQKVAIKKVHNIF 142

HSP 3 Score: 31.187 bits (69), Expect = 5.186e-20
Identity = 13/18 (72.22%), Postives = 16/18 (88.89%), Query Frame = 1

Query:  442 LSEEHCQYFLYQLLRGLK 495
            L++EH Q+FLYQLLR LK
Sbjct:  205 LTKEHYQFFLYQLLRALK 222

[Close]

BLAST of CF504403 vs. ExPASy Swiss-Prot
Match: MPK10_ORYSJ (Mitogen-activated protein kinase 10 OS=Oryza sativa subsp. japonica GN=MPK10 PE=2 SV=2)

HSP 1 Score: 67.0106 bits (162), Expect = 5.209e-20
Identity = 28/61 (45.90%), Postives = 46/61 (75.41%), Query Frame = 2

Query:  254 FDNHMDAKRTLREIKLLQHFDHENVIAVKDVVPPPLRREFTDVYIAAELMDTDLYQIIRSN 436
            F++  DA R LREIKLL+   H +++ +K ++ PP RR+F D+Y+  ELM++DL+Q+I++N
Sbjct:   60 FEHISDAARILREIKLLRLLRHPDIVEIKHIMLPPSRRDFKDIYVVFELMESDLHQVIKAN 120

HSP 2 Score: 38.5058 bits (88), Expect = 5.209e-20
Identity = 16/30 (53.33%), Postives = 23/30 (76.67%), Query Frame = 1

Query:  166 IGRGAYGIVCSVLNTETNELVAMKKIANRF 255
            IG+G+YG+VCS ++  T E VA+KKI + F
Sbjct:   31 IGKGSYGVVCSAIDVHTGEKVAIKKIHDIF 60

HSP 3 Score: 31.187 bits (69), Expect = 5.209e-20
Identity = 13/18 (72.22%), Postives = 16/18 (88.89%), Query Frame = 1

Query:  442 LSEEHCQYFLYQLLRGLK 495
            L++EH Q+FLYQLLR LK
Sbjct:  123 LTKEHYQFFLYQLLRALK 140

[Close]

BLAST of CF504403 vs. ExPASy Swiss-Prot
Match: MPK19_ARATH (Mitogen-activated protein kinase 19 OS=Arabidopsis thaliana GN=MPK19 PE=2 SV=2)

HSP 1 Score: 66.6254 bits (161), Expect = 6.755e-20
Identity = 27/61 (44.26%), Postives = 46/61 (75.41%), Query Frame = 2

Query:  254 FDNHMDAKRTLREIKLLQHFDHENVIAVKDVVPPPLRREFTDVYIAAELMDTDLYQIIRSN 436
            F++  DA R LRE+KLL+   H +++ +K ++ PP +REF D+Y+  ELM++DL+Q+I++N
Sbjct:   60 FEHVSDALRILREVKLLRLLRHPDIVEIKSIMLPPSKREFKDIYVVFELMESDLHQVIKAN 120

HSP 2 Score: 40.0466 bits (92), Expect = 6.755e-20
Identity = 16/30 (53.33%), Postives = 25/30 (83.33%), Query Frame = 1

Query:  166 IGRGAYGIVCSVLNTETNELVAMKKIANRF 255
            IG+G+YG+VC+ ++T+T E VA+KKI + F
Sbjct:   31 IGKGSYGVVCAAIDTQTGEKVAIKKINDVF 60

HSP 3 Score: 29.6462 bits (65), Expect = 6.755e-20
Identity = 12/18 (66.67%), Postives = 15/18 (83.33%), Query Frame = 1

Query:  442 LSEEHCQYFLYQLLRGLK 495
            L+ EH Q+FLYQ+LR LK
Sbjct:  123 LTREHHQFFLYQMLRALK 140

[Close]

BLAST of CF504403 vs. ExPASy Swiss-Prot
Match: MPK18_ARATH (Mitogen-activated protein kinase 18 OS=Arabidopsis thaliana GN=MPK18 PE=1 SV=3)

HSP 1 Score: 66.6254 bits (161), Expect = 1.134e-19
Identity = 27/61 (44.26%), Postives = 46/61 (75.41%), Query Frame = 2

Query:  254 FDNHMDAKRTLREIKLLQHFDHENVIAVKDVVPPPLRREFTDVYIAAELMDTDLYQIIRSN 436
            F++  DA R LRE+KLL+   H +++ +K ++ PP +REF D+Y+  ELM++DL+Q+I++N
Sbjct:   48 FEHISDALRILREVKLLRLLRHPDIVEIKSIMLPPSKREFKDIYVVFELMESDLHQVIKAN 108

HSP 2 Score: 39.2762 bits (90), Expect = 1.134e-19
Identity = 16/30 (53.33%), Postives = 24/30 (80.00%), Query Frame = 1

Query:  166 IGRGAYGIVCSVLNTETNELVAMKKIANRF 255
            IG+G+YG+VC+ ++T T E VA+KKI + F
Sbjct:   19 IGKGSYGVVCAAIDTHTGEKVAIKKINDVF 48

HSP 3 Score: 29.6462 bits (65), Expect = 1.134e-19
Identity = 12/18 (66.67%), Postives = 15/18 (83.33%), Query Frame = 1

Query:  442 LSEEHCQYFLYQLLRGLK 495
            L+ EH Q+FLYQ+LR LK
Sbjct:  111 LTREHHQFFLYQMLRALK 128

[Close]

BLAST of CF504403 vs. ExPASy Swiss-Prot
Match: MAPK_FUSSO (Mitogen-activated protein kinase OS=Fusarium solani subsp. pisi GN=MAPK PE=2 SV=1)

HSP 1 Score: 64.3142 bits (155), Expect = 5.470e-19
Identity = 28/63 (44.44%), Postives = 45/63 (71.43%), Query Frame = 2

Query:  254 FDNHMDAKRTLREIKLLQHFDHENVIAVKDVVPPPLRREFTDVYIAAELMDTDLYQIIRSNQV 442
            FD+ M   RTLRE+KLL++F+HEN+I++ D+  P     F +VY+  ELM+TD+++ IR+  +
Sbjct:   57 FDHSMFCLRTLREMKLLRYFNHENIISILDIQKPRNYESFNEVYLIQELMETDMHRAIRTQDL 119

HSP 2 Score: 34.6538 bits (78), Expect = 5.470e-19
Identity = 13/18 (72.22%), Postives = 16/18 (88.89%), Query Frame = 1

Query:  442 LSEEHCQYFLYQLLRGLK 495
            LS++HCQYF+YQ LR LK
Sbjct:  119 LSDDHCQYFIYQTLRALK 136

HSP 3 Score: 34.2686 bits (77), Expect = 5.470e-19
Identity = 13/26 (50.00%), Postives = 20/26 (76.92%), Query Frame = 1

Query:  166 IGRGAYGIVCSVLNTETNELVAMKKI 243
            +G GAYG+VCS ++  + + VA+KKI
Sbjct:   29 VGEGAYGVVCSAIHKPSGQKVAIKKI 54

[Close]

BLAST of CF504403 vs. ExPASy Swiss-Prot
Match: MPK16_ORYSJ (Mitogen-activated protein kinase 16 OS=Oryza sativa subsp. japonica GN=MPK16 PE=2 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 1.174e-18
Identity = 30/61 (49.18%), Postives = 46/61 (75.41%), Query Frame = 2

Query:  254 FDNHMDAKRTLREIKLLQHFDHENVIAVKDVVPPPLRREFTDVYIAAELMDTDLYQIIRSN 436
            F++  DA R LREIKLL+   H +++ +K ++ PP RREF D+YI  ELM++DL+Q+I++N
Sbjct:   57 FEHISDATRILREIKLLRLLRHPDIVEIKHIMLPPSRREFRDIYIIFELMESDLHQVIKAN 117

HSP 2 Score: 32.3426 bits (72), Expect = 1.174e-18
Identity = 13/30 (43.33%), Postives = 22/30 (73.33%), Query Frame = 1

Query:  166 IGRGAYGIVCSVLNTETNELVAMKKIANRF 255
            +G+G+YG+V + ++T T   VA+KKI + F
Sbjct:   28 VGKGSYGVVAAAVDTHTGGRVAIKKINDVF 57

HSP 3 Score: 31.5722 bits (70), Expect = 1.174e-18
Identity = 13/18 (72.22%), Postives = 16/18 (88.89%), Query Frame = 1

Query:  442 LSEEHCQYFLYQLLRGLK 495
            L+ EH Q+FLYQLLRG+K
Sbjct:  120 LTPEHHQFFLYQLLRGMK 137

[Close]

BLAST of CF504403 vs. ExPASy Swiss-Prot
Match: MPK11_ORYSJ (Mitogen-activated protein kinase 11 OS=Oryza sativa subsp. japonica GN=MPK11 PE=2 SV=2)

HSP 1 Score: 63.5438 bits (153), Expect = 1.961e-18
Identity = 26/61 (42.62%), Postives = 45/61 (73.77%), Query Frame = 2

Query:  254 FDNHMDAKRTLREIKLLQHFDHENVIAVKDVVPPPLRREFTDVYIAAELMDTDLYQIIRSN 436
            F++  DA R LREIKLL+   H +++ +K ++ PP + +F D+Y+  ELM++DL+Q+I++N
Sbjct:   61 FEHISDAARILREIKLLRLLRHPDIVEIKHIMLPPSKMDFRDIYVVFELMESDLHQVIKAN 121

HSP 2 Score: 37.7354 bits (86), Expect = 1.961e-18
Identity = 17/30 (56.67%), Postives = 22/30 (73.33%), Query Frame = 1

Query:  166 IGRGAYGIVCSVLNTETNELVAMKKIANRF 255
            IG+G+YG+VCS  +  T E VA+KKI N F
Sbjct:   32 IGKGSYGLVCSANDIHTGEKVAIKKIHNIF 61

HSP 3 Score: 30.0314 bits (66), Expect = 1.961e-18
Identity = 12/18 (66.67%), Postives = 15/18 (83.33%), Query Frame = 1

Query:  442 LSEEHCQYFLYQLLRGLK 495
            L+ EH Q+FLYQ+LR LK
Sbjct:  124 LTREHYQFFLYQMLRALK 141

[Close]

BLAST of CF504403 vs. ExPASy Swiss-Prot
Match: CPK1_CRYNE (Mitogen-activated protein kinase CPK1 OS=Cryptococcus neoformans GN=CPK1 PE=3 SV=1)

HSP 1 Score: 73.559 bits (179), Expect = 5.244e-18
Identity = 32/63 (50.79%), Postives = 48/63 (76.19%), Query Frame = 2

Query:  254 FDNHMDAKRTLREIKLLQHFDHENVIAVKDVVPPPLRREFTDVYIAAELMDTDLYQIIRSNQV 442
            FD+ M  +RTLREIKLL+HF HEN+I++ D++ P     F +VY+  ELM+TDL+++IRS ++
Sbjct:   50 FDHTMFCQRTLREIKLLRHFHHENIISILDLIQPESYEVFNEVYLVQELMETDLHRVIRSQEL 112

HSP 2 Score: 36.5798 bits (83), Expect = 5.244e-18
Identity = 14/18 (77.78%), Postives = 17/18 (94.44%), Query Frame = 1

Query:  442 LSEEHCQYFLYQLLRGLK 495
            LS++HCQYF+YQ LRGLK
Sbjct:  112 LSDDHCQYFVYQTLRGLK 129

[Close]

BLAST of CF504403 vs. ExPASy Swiss-Prot
Match: FUS3_YEAST (Mitogen-activated protein kinase FUS3 OS=Saccharomyces cerevisiae GN=FUS3 PE=1 SV=2)

HSP 1 Score: 63.1586 bits (152), Expect = 5.623e-18
Identity = 29/63 (46.03%), Postives = 42/63 (66.67%), Query Frame = 2

Query:  254 FDNHMDAKRTLREIKLLQHFDHENVIAVKDVVPPPLRREFTDVYIAAELMDTDLYQIIRSNQV 442
            FD  + A RTLREIK+L+HF HEN+I + ++  P     F +VYI  ELM TDL+++I +  +
Sbjct:   47 FDKPLFALRTLREIKILKHFKHENIITIFNIQRPDSFENFNEVYIIQELMQTDLHRVISTQML 109

HSP 2 Score: 36.965 bits (84), Expect = 5.623e-18
Identity = 15/26 (57.69%), Postives = 20/26 (76.92%), Query Frame = 1

Query:  166 IGRGAYGIVCSVLNTETNELVAMKKI 243
            +G GAYG+VCS  +  T E+VA+KKI
Sbjct:   19 LGEGAYGVVCSATHKPTGEIVAIKKI 44

HSP 3 Score: 29.6462 bits (65), Expect = 5.623e-18
Identity = 11/18 (61.11%), Postives = 15/18 (83.33%), Query Frame = 1

Query:  442 LSEEHCQYFLYQLLRGLK 495
            LS++H QYF+YQ LR +K
Sbjct:  109 LSDDHIQYFIYQTLRAVK 126

[Close]

BLAST of CF504403 vs. ExPASy Swiss-Prot
Match: MPK13_ORYSJ (Mitogen-activated protein kinase 13 OS=Oryza sativa subsp. japonica GN=MPK13 PE=2 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 2.021e-17
Identity = 28/61 (45.90%), Postives = 45/61 (73.77%), Query Frame = 2

Query:  254 FDNHMDAKRTLREIKLLQHFDHENVIAVKDVVPPPLRREFTDVYIAAELMDTDLYQIIRSN 436
            F++  DA R LREIK+L+   H +++ +K ++ PP RREF D+Y+  ELM++DL+Q+I +N
Sbjct:   48 FEHVSDAIRILREIKVLRLLRHPDIVVIKHIMLPPTRREFRDIYVVFELMESDLHQVIEAN 108

HSP 2 Score: 35.039 bits (79), Expect = 2.021e-17
Identity = 14/30 (46.67%), Postives = 23/30 (76.67%), Query Frame = 1

Query:  166 IGRGAYGIVCSVLNTETNELVAMKKIANRF 255
            +G+G+YG+V + ++T T E VA+KKI + F
Sbjct:   19 VGKGSYGVVAAAVDTHTGERVAIKKINDVF 48

HSP 3 Score: 26.5646 bits (57), Expect = 2.021e-17
Identity = 12/18 (66.67%), Postives = 14/18 (77.78%), Query Frame = 1

Query:  442 LSEEHCQYFLYQLLRGLK 495
            LS EH ++FLYQLL  LK
Sbjct:  111 LSPEHHRFFLYQLLCALK 128

The following BLAST results are available for this feature:

BLAST of CF504403 vs. ExPASy Swiss-Prot

BLAST of CF504403 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 93


Match Name	E-value	Identity	Description
MPK9_ORYSJ	5.186e-20	45.90	Mitogen-activated protein kinase 9 OS=Oryza sativa...	[more]
MPK10_ORYSJ	5.209e-20	45.90	Mitogen-activated protein kinase 10 OS=Oryza sativ...	[more]
MPK19_ARATH	6.755e-20	44.26	Mitogen-activated protein kinase 19 OS=Arabidopsis...	[more]
MPK18_ARATH	1.134e-19	44.26	Mitogen-activated protein kinase 18 OS=Arabidopsis...	[more]
MAPK_FUSSO	5.470e-19	44.44	Mitogen-activated protein kinase OS=Fusarium solan...	[more]
MPK16_ORYSJ	1.174e-18	49.18	Mitogen-activated protein kinase 16 OS=Oryza sativ...	[more]
MPK11_ORYSJ	1.961e-18	42.62	Mitogen-activated protein kinase 11 OS=Oryza sativ...	[more]
CPK1_CRYNE	5.244e-18	50.79	Mitogen-activated protein kinase CPK1 OS=Cryptococ...	[more]
FUS3_YEAST	5.623e-18	46.03	Mitogen-activated protein kinase FUS3 OS=Saccharom...	[more]
MPK13_ORYSJ	2.021e-17	45.90	Mitogen-activated protein kinase 13 OS=Oryza sativ...	[more]

Pages

Properties


Property Name	Value
Genbank description	USDA-FP_119000-892 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-17_H10 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CF504403 ID=CF504403; Name=CF504403; organism=Citrus sinensis; type=EST; length=495bp
GAGAAAAAGAGAGAGAGTAAAATGGCTGACGTGGCGCAGGTCAACGGCGT
AGGTCAAACGGCTGATTTTCCTGCGGTACCGACGCACGGCGGTCAGTTTA
TACAGTACAATATATTTGGAAACTTGTTTGAAATCACGGCCAAGTATCGG
CCTCCGATCATGCCGATTGGTCGCGGCGCGTACGGGATCGTTTGCTCGGT
GTTGAATACGGAGACGAATGAGCTCGTTGCGATGAAGAAAATAGCGAATC
GCTTTTGATAATCACATGGATGCCAAGCGAACGCTTCGTGAGATTAAGCT
TCTGCAACATTTCGATCATGAAAATGTGATAGCTGTAAAAGATGTGGTTC
CCCCACCGTTACGAAGAGAATTCACTGATGTCTATATTGCTGCGGAACTC
ATGGACACTGACCTTTACCAAATTATTCGCTCAAATCAAGTTTATCCGAG
GAGCACTGCCAGTATTTCTTGTATCAACTTCTTCGAGGACTCAAG