CF504403 | Citrus Genome Database

Overview


Name	CF504403
Unique Name	CF504403
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	495

Libraries


Library Name	Type
Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of CF504403 vs. ExPASy Swiss-Prot
Match: MPK12_ORYSJ (Mitogen-activated protein kinase 12 OS=Oryza sativa subsp. japonica GN=MPK12 PE=1 SV=2)

HSP 1 Score: 69.3218 bits (168), Expect = 1.059e-11
Identity = 30/61 (49.18%), Postives = 46/61 (75.41%), Query Frame = 2

Query:  254 FDNHMDAKRTLREIKLLQHFDHENVIAVKDVVPPPLRREFTDVYIAAELMDTDLYQIIRSN 436
            F++  DA R LREIKLL+   H +++ +K ++ PP RREF D+Y+  ELM++DL+Q+IR+N
Sbjct:  122 FEHVSDATRILREIKLLRLLRHPDIVEIKHIMLPPSRREFQDIYVVFELMESDLHQVIRAN 182

[Close]

BLAST of CF504403 vs. ExPASy Swiss-Prot
Match: JNK_DROME (Stress-activated protein kinase JNK OS=Drosophila melanogaster GN=bsk PE=1 SV=1)

HSP 1 Score: 49.2914 bits (116), Expect = 1.332e-11
Identity = 26/67 (38.81%), Postives = 39/67 (58.21%), Query Frame = 2

Query:  254 FDNHMDAKRTLREIKLLQHFDHENVIAVKDVVPPPLR-REFTDVYIAAELMDTDLYQIIRSNQVYPR 451
            F N   AKR  RE KL++  +H+N+I + +   P     EF DVY+  ELMD +L Q+I+ +  + R
Sbjct:   59 FQNVTHAKRAYREFKLMKLVNHKNIIGLLNAFTPQRNLEEFQDVYLVMELMDANLCQVIQMDLDHDR 125

HSP 2 Score: 36.1946 bits (82), Expect = 1.332e-11
Identity = 16/33 (48.48%), Postives = 23/33 (69.70%), Query Frame = 1

Query:  157 IMPIGRGAYGIVCSVLNTETNELVAMKKIANRF 255
            + PIG GA GIVC+  +T T + VA+KK++  F
Sbjct:   27 LRPIGSGAQGIVCAAYDTITQQNVAIKKLSRPF 59

HSP 3 Score: 22.3274 bits (46), Expect = 1.332e-11
Identity = 8/22 (36.36%), Postives = 13/22 (59.09%), Query Frame = 1

Query:  430 LKSSLSEEHCQYFLYQLLRGLK 495
            ++  L  +   Y LYQ+L G+K
Sbjct:  117 IQMDLDHDRMSYLLYQMLCGIK 138

[Close]

BLAST of CF504403 vs. ExPASy Swiss-Prot
Match: MK08_RAT (Mitogen-activated protein kinase 8 OS=Rattus norvegicus GN=Mapk8 PE=1 SV=1)

HSP 1 Score: 50.0618 bits (118), Expect = 1.712e-11
Identity = 26/60 (43.33%), Postives = 37/60 (61.67%), Query Frame = 2

Query:  254 FDNHMDAKRTLREIKLLQHFDHENVIAVKDV-VPPPLRREFTDVYIAAELMDTDLYQIIR 430
            F N   AKR  RE+ L++  +H+N+I + +V  P     EF DVYI  ELMD +L Q+I+
Sbjct:   61 FQNQTHAKRAYRELVLMKCVNHKNIIGLLNVFTPQKSLEEFQDVYIVMELMDANLCQVIQ 120

HSP 2 Score: 33.8834 bits (76), Expect = 1.712e-11
Identity = 18/53 (33.96%), Postives = 28/53 (52.83%), Query Frame = 1

Query:   97 FIQYNIFGNLFEITAKYRPPIMPIGRGAYGIVCSVLNTETNELVAMKKIANRF 255
            F    I  + F +  +Y+  + PIG GA GIVC+  +      VA+KK++  F
Sbjct:   10 FYSVEIADSTFTVLKRYQN-LKPIGSGAQGIVCAAYDAILERNVAIKKLSRPF 61

HSP 3 Score: 23.483 bits (49), Expect = 1.712e-11
Identity = 9/22 (40.91%), Postives = 13/22 (59.09%), Query Frame = 1

Query:  430 LKSSLSEEHCQYFLYQLLRGLK 495
            ++  L  E   Y LYQ+L G+K
Sbjct:  119 IQMELDHERMSYLLYQMLCGIK 140

[Close]

BLAST of CF504403 vs. ExPASy Swiss-Prot
Match: MK08_DANRE (Mitogen-activated protein kinase 8 OS=Danio rerio GN=mapk8 PE=2 SV=1)

HSP 1 Score: 49.6766 bits (117), Expect = 1.718e-11
Identity = 25/60 (41.67%), Postives = 37/60 (61.67%), Query Frame = 2

Query:  254 FDNHMDAKRTLREIKLLQHFDHENVIAVKDV-VPPPLRREFTDVYIAAELMDTDLYQIIR 430
            F N   AKR  RE+ L++  +H+N+I + +V  P     EF DVY+  ELMD +L Q+I+
Sbjct:   61 FQNQTHAKRAYRELVLMKCVNHKNIIGLLNVFTPQKTLEEFQDVYLVMELMDANLCQVIQ 120

HSP 2 Score: 34.2686 bits (77), Expect = 1.718e-11
Identity = 17/54 (31.48%), Postives = 31/54 (57.41%), Query Frame = 1

Query:   94 QFIQYNIFGNLFEITAKYRPPIMPIGRGAYGIVCSVLNTETNELVAMKKIANRF 255
            ++   ++  + F +  +Y+  + PIG GA GIVCS  +   +  VA+KK++  F
Sbjct:    9 EYYSIDVGDSTFTVLKRYQN-LRPIGSGAQGIVCSAYDHVLDRNVAIKKLSRPF 61

HSP 3 Score: 23.483 bits (49), Expect = 1.718e-11
Identity = 9/22 (40.91%), Postives = 13/22 (59.09%), Query Frame = 1

Query:  430 LKSSLSEEHCQYFLYQLLRGLK 495
            ++  L  E   Y LYQ+L G+K
Sbjct:  119 IQMELDHERLSYLLYQMLCGIK 140

[Close]

BLAST of CF504403 vs. ExPASy Swiss-Prot
Match: MPK9_ARATH (Mitogen-activated protein kinase 9 OS=Arabidopsis thaliana GN=MPK9 PE=2 SV=2)

HSP 1 Score: 68.5514 bits (166), Expect = 1.806e-11
Identity = 30/61 (49.18%), Postives = 46/61 (75.41%), Query Frame = 2

Query:  254 FDNHMDAKRTLREIKLLQHFDHENVIAVKDVVPPPLRREFTDVYIAAELMDTDLYQIIRSN 436
            F++  DA R LREIKLL+   H +++ +K V+ PP RREF D+Y+  ELM++DL+Q+I++N
Sbjct:   58 FEHVSDATRILREIKLLRLLRHPDIVEIKHVMLPPSRREFRDIYVVFELMESDLHQVIKAN 118

[Close]

BLAST of CF504403 vs. ExPASy Swiss-Prot
Match: MPK8_ARATH (Mitogen-activated protein kinase 8 OS=Arabidopsis thaliana GN=MPK8 PE=2 SV=2)

HSP 1 Score: 68.5514 bits (166), Expect = 1.806e-11
Identity = 30/61 (49.18%), Postives = 46/61 (75.41%), Query Frame = 2

Query:  254 FDNHMDAKRTLREIKLLQHFDHENVIAVKDVVPPPLRREFTDVYIAAELMDTDLYQIIRSN 436
            F++  DA R LREIKLL+   H +V+ +K ++ PP RREF D+Y+  ELM++DL+Q+I++N
Sbjct:  139 FEHVSDATRILREIKLLRLLRHPDVVEIKHIMLPPSRREFRDIYVVFELMESDLHQVIKAN 199

[Close]

BLAST of CF504403 vs. ExPASy Swiss-Prot
Match: MK09_HUMAN (Mitogen-activated protein kinase 9 OS=Homo sapiens GN=MAPK9 PE=1 SV=2)

HSP 1 Score: 50.447 bits (119), Expect = 2.057e-11
Identity = 26/59 (44.07%), Postives = 37/59 (62.71%), Query Frame = 2

Query:  254 FDNHMDAKRTLREIKLLQHFDHENVIAVKDV-VPPPLRREFTDVYIAAELMDTDLYQII 427
            F N   AKR  RE+ LL+  +H+N+I++ +V  P     EF DVY+  ELMD +L Q+I
Sbjct:   61 FQNQTHAKRAYRELVLLKCVNHKNIISLLNVFTPQKTLEEFQDVYLVMELMDANLCQVI 119

HSP 2 Score: 37.3502 bits (85), Expect = 2.057e-11
Identity = 19/54 (35.19%), Postives = 30/54 (55.56%), Query Frame = 1

Query:   94 QFIQYNIFGNLFEITAKYRPPIMPIGRGAYGIVCSVLNTETNELVAMKKIANRF 255
            QF    +  + F +  +Y+  + PIG GA GIVC+  +T     VA+KK++  F
Sbjct:    9 QFYSVQVADSTFTVLKRYQQ-LKPIGSGAQGIVCAAFDTVLGINVAVKKLSRPF 61

[Close]

BLAST of CF504403 vs. ExPASy Swiss-Prot
Match: MK14B_DANRE (Mitogen-activated protein kinase 14B OS=Danio rerio GN=mapk14b PE=1 SV=1)

HSP 1 Score: 55.4546 bits (132), Expect = 2.068e-11
Identity = 28/64 (43.75%), Postives = 39/64 (60.94%), Query Frame = 2

Query:  254 FDNHMDAKRTLREIKLLQHFDHENVIAVKDVVPPPLRRE-FTDVYIAAELMDTDLYQIIRSNQV 442
            F + + AKRT RE++LL+H  HENVI + DV  P    E F DVY+   LM  DL  I++  ++
Sbjct:   60 FQSIIHAKRTYRELRLLKHMKHENVIGLLDVFSPATSLEGFNDVYLVTHLMGADLNNIVKCQKL 123

HSP 2 Score: 32.3426 bits (72), Expect = 2.068e-11
Identity = 16/53 (30.19%), Postives = 30/53 (56.60%), Query Frame = 1

Query:   97 FIQYNIFGNLFEITAKYRPPIMPIGRGAYGIVCSVLNTETNELVAMKKIANRF 255
            F +  +   ++E+  +Y+  + P+G GAYG V S  + +    VA+KK++  F
Sbjct:    9 FYRQELNKTIWEVPERYQN-LSPVGSGAYGSVMSAFDGKAGLRVAVKKLSRPF 60

[Close]

BLAST of CF504403 vs. ExPASy Swiss-Prot
Match: MK08_HUMAN (Mitogen-activated protein kinase 8 OS=Homo sapiens GN=MAPK8 PE=1 SV=2)

HSP 1 Score: 50.0618 bits (118), Expect = 2.208e-11
Identity = 26/60 (43.33%), Postives = 37/60 (61.67%), Query Frame = 2

Query:  254 FDNHMDAKRTLREIKLLQHFDHENVIAVKDV-VPPPLRREFTDVYIAAELMDTDLYQIIR 430
            F N   AKR  RE+ L++  +H+N+I + +V  P     EF DVYI  ELMD +L Q+I+
Sbjct:   61 FQNQTHAKRAYRELVLMKCVNHKNIIGLLNVFTPQKSLEEFQDVYIVMELMDANLCQVIQ 120

HSP 2 Score: 33.4982 bits (75), Expect = 2.208e-11
Identity = 18/53 (33.96%), Postives = 28/53 (52.83%), Query Frame = 1

Query:   97 FIQYNIFGNLFEITAKYRPPIMPIGRGAYGIVCSVLNTETNELVAMKKIANRF 255
            F    I  + F +  +Y+  + PIG GA GIVC+  +      VA+KK++  F
Sbjct:   10 FYSVEIGDSTFTVLKRYQN-LKPIGSGAQGIVCAAYDAILERNVAIKKLSRPF 61

HSP 3 Score: 23.483 bits (49), Expect = 2.208e-11
Identity = 9/22 (40.91%), Postives = 13/22 (59.09%), Query Frame = 1

Query:  430 LKSSLSEEHCQYFLYQLLRGLK 495
            ++  L  E   Y LYQ+L G+K
Sbjct:  119 IQMELDHERMSYLLYQMLCGIK 140

[Close]

BLAST of CF504403 vs. ExPASy Swiss-Prot
Match: MK08_XENLA (Mitogen-activated protein kinase 8 OS=Xenopus laevis GN=mapk8 PE=2 SV=2)

HSP 1 Score: 48.9062 bits (115), Expect = 2.208e-11
Identity = 25/60 (41.67%), Postives = 37/60 (61.67%), Query Frame = 2

Query:  254 FDNHMDAKRTLREIKLLQHFDHENVIAVKDV-VPPPLRREFTDVYIAAELMDTDLYQIIR 430
            F N   AKR  RE+ L++  +H+N+I + +V  P     EF D+YI  ELMD +L Q+I+
Sbjct:   61 FQNQTHAKRAYRELVLMKCVNHKNIIGLLNVFTPQKSLEEFQDLYIVMELMDANLCQVIQ 120

HSP 2 Score: 34.6538 bits (78), Expect = 2.208e-11
Identity = 18/53 (33.96%), Postives = 29/53 (54.72%), Query Frame = 1

Query:   97 FIQYNIFGNLFEITAKYRPPIMPIGRGAYGIVCSVLNTETNELVAMKKIANRF 255
            F  + I  + F +  +Y+  + PIG GA GIVC+  +      VA+KK++  F
Sbjct:   10 FSVFEIGDSTFTVLKRYQN-LKPIGSGAQGIVCAAFDAVLERHVAIKKLSRPF 61

HSP 3 Score: 23.483 bits (49), Expect = 2.208e-11
Identity = 9/22 (40.91%), Postives = 13/22 (59.09%), Query Frame = 1

Query:  430 LKSSLSEEHCQYFLYQLLRGLK 495
            ++  L  E   Y LYQ+L G+K
Sbjct:  119 IQMELDHERMSYLLYQMLCGIK 140

The following BLAST results are available for this feature:

BLAST of CF504403 vs. ExPASy Swiss-Prot

BLAST of CF504403 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 93


Match Name	E-value	Identity	Description
MPK12_ORYSJ	1.059e-11	49.18	Mitogen-activated protein kinase 12 OS=Oryza sativ...	[more]
JNK_DROME	1.332e-11	38.81	Stress-activated protein kinase JNK OS=Drosophila ...	[more]
MK08_RAT	1.712e-11	43.33	Mitogen-activated protein kinase 8 OS=Rattus norve...	[more]
MK08_DANRE	1.718e-11	41.67	Mitogen-activated protein kinase 8 OS=Danio rerio ...	[more]
MPK9_ARATH	1.806e-11	49.18	Mitogen-activated protein kinase 9 OS=Arabidopsis ...	[more]
MPK8_ARATH	1.806e-11	49.18	Mitogen-activated protein kinase 8 OS=Arabidopsis ...	[more]
MK09_HUMAN	2.057e-11	44.07	Mitogen-activated protein kinase 9 OS=Homo sapiens...	[more]
MK14B_DANRE	2.068e-11	43.75	Mitogen-activated protein kinase 14B OS=Danio reri...	[more]
MK08_HUMAN	2.208e-11	43.33	Mitogen-activated protein kinase 8 OS=Homo sapiens...	[more]
MK08_XENLA	2.208e-11	41.67	Mitogen-activated protein kinase 8 OS=Xenopus laev...	[more]

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Properties


Property Name	Value
Genbank description	USDA-FP_119000-892 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-17_H10 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CF504403 ID=CF504403; Name=CF504403; organism=Citrus sinensis; type=EST; length=495bp
GAGAAAAAGAGAGAGAGTAAAATGGCTGACGTGGCGCAGGTCAACGGCGT
AGGTCAAACGGCTGATTTTCCTGCGGTACCGACGCACGGCGGTCAGTTTA
TACAGTACAATATATTTGGAAACTTGTTTGAAATCACGGCCAAGTATCGG
CCTCCGATCATGCCGATTGGTCGCGGCGCGTACGGGATCGTTTGCTCGGT
GTTGAATACGGAGACGAATGAGCTCGTTGCGATGAAGAAAATAGCGAATC
GCTTTTGATAATCACATGGATGCCAAGCGAACGCTTCGTGAGATTAAGCT
TCTGCAACATTTCGATCATGAAAATGTGATAGCTGTAAAAGATGTGGTTC
CCCCACCGTTACGAAGAGAATTCACTGATGTCTATATTGCTGCGGAACTC
ATGGACACTGACCTTTACCAAATTATTCGCTCAAATCAAGTTTATCCGAG
GAGCACTGCCAGTATTTCTTGTATCAACTTCTTCGAGGACTCAAG