CF504462

Overview
NameCF504462
Unique NameCF504462
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length357
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig3352 contig Csv1_Contig3352:20..376. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CF504462 vs. ExPASy Swiss-Prot
Match: EF2_DICDI (Elongation factor 2 OS=Dictyostelium discoideum GN=efbA PE=1 SV=2)

HSP 1 Score: 111.309 bits (277), Expect = 1.567e-24
Identity = 58/121 (47.93%), Postives = 80/121 (66.12%), Query Frame = 2 
Query:    2 KKWTTKNTGSATCK---RGFVQFCYEPIKQIINTCMNDQKDKLWPMLQKLGVTMKSEEKDLMGKALMKRVMQTWLPASSALLEMMIFHLPSPSTAQKYRVENLYEGPLDDQYANAIRNCDP 355
            KKWT+ N  SA  K   R F QF  EPI Q+    +++   KL  M++ L +T+  E+ ++ GK L+K VM+ +LPA+ A+L M++ HLPSP  AQKYR  NLYEGP+DD+ A AI+ CDP
Sbjct:  253 KKWTS-NPQSADGKALPRAFCQFVLEPIYQLTRAIVDEDAVKLEKMMKTLQITLAPEDAEIKGKQLVKAVMRKFLPAADAILSMIVTHLPSPLVAQKYRCANLYEGPMDDECAVAIQKCDP 372
[Close]
BLAST of CF504462 vs. ExPASy Swiss-Prot
Match: EF2_RAT (Elongation factor 2 OS=Rattus norvegicus GN=Eef2 PE=1 SV=4)

HSP 1 Score: 110.538 bits (275), Expect = 2.673e-24
Identity = 52/104 (50.00%), Postives = 72/104 (69.23%), Query Frame = 2 
Query:   44 RGFVQFCYEPIKQIINTCMNDQKDKLWPMLQKLGVTMKSEEKDLMGKALMKRVMQTWLPASSALLEMMIFHLPSPSTAQKYRVENLYEGPLDDQYANAIRNCDP 355
            R F Q   +PI ++ +  MN +K++   +++KL + + SE+KD  GK L+K VM+ WLPA  ALL+M+  HLPSP TAQKYR E LYEGP DD+ A  I++CDP
Sbjct:  287 RTFCQLILDPIFKVFDAIMNFRKEETAKLIEKLDIKLDSEDKDKEGKPLLKAVMRRWLPAGDALLQMITIHLPSPVTAQKYRCELLYEGPPDDEAAMGIKSCDP 390
[Close]
BLAST of CF504462 vs. ExPASy Swiss-Prot
Match: EF2_PONAB (Elongation factor 2 OS=Pongo abelii GN=EEF2 PE=2 SV=3)

HSP 1 Score: 110.538 bits (275), Expect = 2.673e-24
Identity = 52/104 (50.00%), Postives = 72/104 (69.23%), Query Frame = 2 
Query:   44 RGFVQFCYEPIKQIINTCMNDQKDKLWPMLQKLGVTMKSEEKDLMGKALMKRVMQTWLPASSALLEMMIFHLPSPSTAQKYRVENLYEGPLDDQYANAIRNCDP 355
            R F Q   +PI ++ +  MN +K++   +++KL + + SE+KD  GK L+K VM+ WLPA  ALL+M+  HLPSP TAQKYR E LYEGP DD+ A  I++CDP
Sbjct:  287 RTFCQLILDPIFKVFDAIMNFKKEETAKLIEKLDIKLDSEDKDKEGKPLLKAVMRRWLPAGDALLQMITIHLPSPVTAQKYRCELLYEGPPDDEAAMGIKSCDP 390
[Close]
BLAST of CF504462 vs. ExPASy Swiss-Prot
Match: EF2_MOUSE (Elongation factor 2 OS=Mus musculus GN=Eef2 PE=1 SV=2)

HSP 1 Score: 110.538 bits (275), Expect = 2.673e-24
Identity = 52/104 (50.00%), Postives = 72/104 (69.23%), Query Frame = 2 
Query:   44 RGFVQFCYEPIKQIINTCMNDQKDKLWPMLQKLGVTMKSEEKDLMGKALMKRVMQTWLPASSALLEMMIFHLPSPSTAQKYRVENLYEGPLDDQYANAIRNCDP 355
            R F Q   +PI ++ +  MN +K++   +++KL + + SE+KD  GK L+K VM+ WLPA  ALL+M+  HLPSP TAQKYR E LYEGP DD+ A  I++CDP
Sbjct:  287 RTFCQLILDPIFKVFDAIMNFRKEETAKLIEKLDIKLDSEDKDKEGKPLLKAVMRRWLPAGDALLQMITIHLPSPVTAQKYRCELLYEGPPDDEAAMGIKSCDP 390
[Close]
BLAST of CF504462 vs. ExPASy Swiss-Prot
Match: EF2_MESAU (Elongation factor 2 OS=Mesocricetus auratus GN=EEF2 PE=2 SV=3)

HSP 1 Score: 110.538 bits (275), Expect = 2.673e-24
Identity = 52/104 (50.00%), Postives = 72/104 (69.23%), Query Frame = 2 
Query:   44 RGFVQFCYEPIKQIINTCMNDQKDKLWPMLQKLGVTMKSEEKDLMGKALMKRVMQTWLPASSALLEMMIFHLPSPSTAQKYRVENLYEGPLDDQYANAIRNCDP 355
            R F Q   +PI ++ +  MN +K++   +++KL + + SE+KD  GK L+K VM+ WLPA  ALL+M+  HLPSP TAQKYR E LYEGP DD+ A  I++CDP
Sbjct:  287 RTFCQLILDPIFKVFDAIMNFRKEETAKLIEKLDIKLDSEDKDKEGKPLLKAVMRRWLPAGDALLQMITIHLPSPVTAQKYRCELLYEGPPDDEAAMGIKSCDP 390
[Close]
BLAST of CF504462 vs. ExPASy Swiss-Prot
Match: EF2_HUMAN (Elongation factor 2 OS=Homo sapiens GN=EEF2 PE=1 SV=4)

HSP 1 Score: 110.538 bits (275), Expect = 2.673e-24
Identity = 52/104 (50.00%), Postives = 72/104 (69.23%), Query Frame = 2 
Query:   44 RGFVQFCYEPIKQIINTCMNDQKDKLWPMLQKLGVTMKSEEKDLMGKALMKRVMQTWLPASSALLEMMIFHLPSPSTAQKYRVENLYEGPLDDQYANAIRNCDP 355
            R F Q   +PI ++ +  MN +K++   +++KL + + SE+KD  GK L+K VM+ WLPA  ALL+M+  HLPSP TAQKYR E LYEGP DD+ A  I++CDP
Sbjct:  287 RTFCQLILDPIFKVFDAIMNFKKEETAKLIEKLDIKLDSEDKDKEGKPLLKAVMRRWLPAGDALLQMITIHLPSPVTAQKYRCELLYEGPPDDEAAMGIKSCDP 390
[Close]
BLAST of CF504462 vs. ExPASy Swiss-Prot
Match: EF2_CALJA (Elongation factor 2 OS=Callithrix jacchus GN=EEF2 PE=2 SV=1)

HSP 1 Score: 110.538 bits (275), Expect = 2.673e-24
Identity = 52/104 (50.00%), Postives = 72/104 (69.23%), Query Frame = 2 
Query:   44 RGFVQFCYEPIKQIINTCMNDQKDKLWPMLQKLGVTMKSEEKDLMGKALMKRVMQTWLPASSALLEMMIFHLPSPSTAQKYRVENLYEGPLDDQYANAIRNCDP 355
            R F Q   +PI ++ +  MN +K++   +++KL + + SE+KD  GK L+K VM+ WLPA  ALL+M+  HLPSP TAQKYR E LYEGP DD+ A  I++CDP
Sbjct:  287 RTFCQLILDPIFKVFDAIMNFKKEETAKLIEKLDIKLDSEDKDKEGKPLLKAVMRRWLPAGDALLQMITIHLPSPVTAQKYRCELLYEGPPDDEAAMGIKSCDP 390
[Close]
BLAST of CF504462 vs. ExPASy Swiss-Prot
Match: EF2_BOVIN (Elongation factor 2 OS=Bos taurus GN=EEF2 PE=2 SV=3)

HSP 1 Score: 110.538 bits (275), Expect = 2.673e-24
Identity = 52/104 (50.00%), Postives = 72/104 (69.23%), Query Frame = 2 
Query:   44 RGFVQFCYEPIKQIINTCMNDQKDKLWPMLQKLGVTMKSEEKDLMGKALMKRVMQTWLPASSALLEMMIFHLPSPSTAQKYRVENLYEGPLDDQYANAIRNCDP 355
            R F Q   +PI ++ +  MN +K++   +++KL + + SE+KD  GK L+K VM+ WLPA  ALL+M+  HLPSP TAQKYR E LYEGP DD+ A  I++CDP
Sbjct:  287 RTFCQLILDPIFKVFDAIMNFKKEETAKLIEKLDIKLDSEDKDKEGKPLLKAVMRRWLPAGDALLQMITIHLPSPVTAQKYRCELLYEGPPDDEAAMGIKSCDP 390
[Close]
BLAST of CF504462 vs. ExPASy Swiss-Prot
Match: EF2_CRIGR (Elongation factor 2 OS=Cricetulus griseus GN=EEF2 PE=3 SV=3)

HSP 1 Score: 110.153 bits (274), Expect = 3.491e-24
Identity = 52/104 (50.00%), Postives = 72/104 (69.23%), Query Frame = 2 
Query:   44 RGFVQFCYEPIKQIINTCMNDQKDKLWPMLQKLGVTMKSEEKDLMGKALMKRVMQTWLPASSALLEMMIFHLPSPSTAQKYRVENLYEGPLDDQYANAIRNCDP 355
            R F Q   +PI ++ +  MN +K++   +++KL + + SE+KD  GK L+K VM+ WLPA  ALL+M+  HLPSP TAQKYR E LYEGP DD+ A  I++CDP
Sbjct:  287 RTFCQLILDPIFKVFDPIMNFRKEETAKLIEKLDIKLDSEDKDKEGKPLLKAVMRRWLPAGDALLQMITIHLPSPVTAQKYRCELLYEGPPDDEAAMGIKSCDP 390
[Close]
BLAST of CF504462 vs. ExPASy Swiss-Prot
Match: EF2_CHICK (Elongation factor 2 OS=Gallus gallus GN=EEF2 PE=1 SV=3)

HSP 1 Score: 109.768 bits (273), Expect = 4.559e-24
Identity = 52/104 (50.00%), Postives = 71/104 (68.27%), Query Frame = 2 
Query:   44 RGFVQFCYEPIKQIINTCMNDQKDKLWPMLQKLGVTMKSEEKDLMGKALMKRVMQTWLPASSALLEMMIFHLPSPSTAQKYRVENLYEGPLDDQYANAIRNCDP 355
            R F Q   +PI ++ +  M  +K++   +++KL + + SE+KD  GK L+K VM+ WLPA  ALL+M+  HLPSP TAQKYR E LYEGP DD+ A  I+NCDP
Sbjct:  287 RTFCQLILDPIFKVFDAIMTFKKEEAAKLIEKLDIKLDSEDKDKEGKPLLKAVMRRWLPAGDALLQMITIHLPSPVTAQKYRCELLYEGPPDDEAAIGIKNCDP 390
The following BLAST results are available for this feature:
BLAST of CF504462 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 36
Match NameE-valueIdentityDescription
EF2_DICDI1.567e-2447.93Elongation factor 2 OS=Dictyostelium discoideum GN... [more]
EF2_RAT2.673e-2450.00Elongation factor 2 OS=Rattus norvegicus GN=Eef2 P... [more]
EF2_PONAB2.673e-2450.00Elongation factor 2 OS=Pongo abelii GN=EEF2 PE=2 S... [more]
EF2_MOUSE2.673e-2450.00Elongation factor 2 OS=Mus musculus GN=Eef2 PE=1 S... [more]
EF2_MESAU2.673e-2450.00Elongation factor 2 OS=Mesocricetus auratus GN=EEF... [more]
EF2_HUMAN2.673e-2450.00Elongation factor 2 OS=Homo sapiens GN=EEF2 PE=1 S... [more]
EF2_CALJA2.673e-2450.00Elongation factor 2 OS=Callithrix jacchus GN=EEF2 ... [more]
EF2_BOVIN2.673e-2450.00Elongation factor 2 OS=Bos taurus GN=EEF2 PE=2 SV=... [more]
EF2_CRIGR3.491e-2450.00Elongation factor 2 OS=Cricetulus griseus GN=EEF2 ... [more]
EF2_CHICK4.559e-2450.00Elongation factor 2 OS=Gallus gallus GN=EEF2 PE=1 ... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_119000-951 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-18_F02 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF504462 ID=CF504462; Name=CF504462; organism=Citrus sinensis; type=EST; length=357bp
AAAGAAGTGGACAACCAAGAACACTGGTAGTGCGACATGCAAGCGTGGTT
TTGTTCAGTTTTGCTATGAACCGATTAAGCAGATTATCAACACTTGCATG
AATGATCAGAAAGATAAGCTGTGGCCCATGTTGCAAAAGCTTGGTGTCAC
GATGAAGTCAGAAGAGAAGGATTTGATGGGCAAGGCATTGATGAAGCGTG
TCATGCAGACCTGGCTTCCTGCAAGTAGTGCACTTTTGGAAATGATGATC
TTTCACCTTCCCTCTCCTTCCACGGCCCAAAAATATCGTGTTGAGAACTT
GTATGAGGGTCCTCTTGATGATCAATATGCTAATGCTATTCGGAACTGTG
ATCCTAA
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