CF504464

Overview
NameCF504464
Unique NameCF504464
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length384
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CF504464 vs. ExPASy Swiss-Prot
Match: GBLP_NEUCR (Guanine nucleotide-binding protein subunit beta-like protein OS=Neurospora crassa GN=cpc-2 PE=3 SV=1)

HSP 1 Score: 131.724 bits (330), Expect = 2.043e-42
Identity = 57/82 (69.51%), Postives = 64/82 (78.05%), Query Frame = 3 
Query:  138 HTEWVSCVRFSPNTLQPTIVSASWDRTVKVWNLSNCKLRATLAGHTGYVNTVAVSPDGSLCASGGKDGVILLWDLAEGKRLY 383
            HTEWVSCVRFSPN   P IVS+ WD+ VKVW LS+CKL+    GHTGY+N V +SPDGSLCASGGKDG  +LWDL E K LY
Sbjct:  147 HTEWVSCVRFSPNPQNPVIVSSGWDKLVKVWELSSCKLQTDHIGHTGYINAVTISPDGSLCASGGKDGTTMLWDLNESKHLY 228

HSP 2 Score: 60.4622 bits (145), Expect = 2.043e-42
Identity = 28/32 (87.50%), Postives = 29/32 (90.62%), Query Frame = 1 
Query:    1 GHTKDVLSVAFSIDNRQIVSASRDRTIKLWNT 96
            GHT DVLSV+FS DNRQIVS SRDRTIKLWNT
Sbjct:  103 GHTNDVLSVSFSADNRQIVSGSRDRTIKLWNT 134
[Close]
BLAST of CF504464 vs. ExPASy Swiss-Prot
Match: GBLP_ORYSJ (Guanine nucleotide-binding protein subunit beta-like protein OS=Oryza sativa subsp. japonica GN=Os01g0686800 PE=1 SV=1)

HSP 1 Score: 157.918 bits (398), Expect = 1.459e-38
Identity = 72/85 (84.71%), Postives = 74/85 (87.06%), Query Frame = 3 
Query:  129 GDAHTEWVSCVRFSPNTLQPTIVSASWDRTVKVWNLSNCKLRATLAGHTGYVNTVAVSPDGSLCASGGKDGVILLWDLAEGKRLY 383
            G+ H  WVSCVRFSPNT QPTIVS SWDRTVKVWNL+NCKLR  L GH GYVN VAVSPDGSLCASGGKDGV LLWDLAEGKRLY
Sbjct:  161 GEGHNGWVSCVRFSPNTFQPTIVSGSWDRTVKVWNLTNCKLRCNLEGHGGYVNAVAVSPDGSLCASGGKDGVTLLWDLAEGKRLY 245

HSP 2 Score: 65.4698 bits (158), Expect = 9.817e-11
Identity = 40/74 (54.05%), Postives = 45/74 (60.81%), Query Frame = 1 
Query:    1 GHTKDVLSVAFSIDNRQIVSASRDRTIKLWNTARRVQVHRFKRETLTLSGFLVLGS------APTRFSPLSFPP 204
            GH KDVLSVAFS+DNRQIVSASRDRTIKLWNT    +         T+ G L  G       +  RFSP +F P
Sbjct:  115 GHDKDVLSVAFSVDNRQIVSASRDRTIKLWNTLGECK--------YTIGGDLGGGEGHNGWVSCVRFSPNTFQP 180
[Close]
BLAST of CF504464 vs. ExPASy Swiss-Prot
Match: GBLP_DICDI (Guanine nucleotide-binding protein subunit beta-like protein OS=Dictyostelium discoideum GN=gpbB PE=1 SV=2)

HSP 1 Score: 120.553 bits (301), Expect = 4.754e-38
Identity = 54/84 (64.29%), Postives = 64/84 (76.19%), Query Frame = 3 
Query:  132 DAHTEWVSCVRFSPNTLQPTIVSASWDRTVKVWNLSNCKLRATLAGHTGYVNTVAVSPDGSLCASGGKDGVILLWDLAEGKRLY 383
            +AH +WVSC+RFSPNT  PTIVS SWD  VK+W++ + K   TL  HTGYVNTV +SPDGSLCASGGKD    LW+L+ GK LY
Sbjct:  153 EAHQDWVSCIRFSPNT--PTIVSGSWDNKVKIWDIKSFKCNHTLTDHTGYVNTVTISPDGSLCASGGKDTFACLWELSSGKPLY 234

HSP 2 Score: 56.9954 bits (136), Expect = 4.754e-38
Identity = 25/32 (78.12%), Postives = 29/32 (90.62%), Query Frame = 1 
Query:    1 GHTKDVLSVAFSIDNRQIVSASRDRTIKLWNT 96
            GHT+DV+SVAFS DNRQI+S SRD TIK+WNT
Sbjct:  110 GHTQDVMSVAFSSDNRQIISGSRDATIKVWNT 141
[Close]
BLAST of CF504464 vs. ExPASy Swiss-Prot
Match: GBLP_SCHPO (Guanine nucleotide-binding protein subunit beta-like protein OS=Schizosaccharomyces pombe GN=rkp1 PE=1 SV=3)

HSP 1 Score: 116.701 bits (291), Expect = 9.422e-36
Identity = 51/82 (62.20%), Postives = 61/82 (74.39%), Query Frame = 3 
Query:  138 HTEWVSCVRFSPNTLQPTIVSASWDRTVKVWNLSNCKLRATLAGHTGYVNTVAVSPDGSLCASGGKDGVILLWDLAEGKRLY 383
            H++WVSCVRFSPN    T VSA WD+ VKVW+L    LR +  GHTGYV+ V +SPDGSLCASGG+DG ++LWDL E   LY
Sbjct:  147 HSDWVSCVRFSPNPDNLTFVSAGWDKAVKVWDLETFSLRTSHYGHTGYVSAVTISPDGSLCASGGRDGTLMLWDLNESTHLY 228

HSP 2 Score: 53.1434 bits (126), Expect = 9.422e-36
Identity = 23/31 (74.19%), Postives = 27/31 (87.10%), Query Frame = 1 
Query:    1 GHTKDVLSVAFSIDNRQIVSASRDRTIKLWN 93
            GHT DVLSV+ S DNRQ+VS SRD+TIK+WN
Sbjct:  103 GHTSDVLSVSISPDNRQVVSGSRDKTIKIWN 133
[Close]
BLAST of CF504464 vs. ExPASy Swiss-Prot
Match: GBLP_LEIMA (Guanine nucleotide-binding protein subunit beta-like protein OS=Leishmania major PE=2 SV=1)

HSP 1 Score: 115.931 bits (289), Expect = 1.325e-34
Identity = 50/84 (59.52%), Postives = 63/84 (75.00%), Query Frame = 3 
Query:  132 DAHTEWVSCVRFSPNTLQPTIVSASWDRTVKVWNLSNCKLRATLAGHTGYVNTVAVSPDGSLCASGGKDGVILLWDLAEGKRLY 383
            D H +WVS + FSP+   P +VS SWD T+KVWN++  K   TL GH+ YV+TV VSPDGSLCASGGKDG  LLWDL+ G++L+
Sbjct:  147 DGHEDWVSSICFSPSLEHPIVVSGSWDNTIKVWNVNGGKCERTLKGHSNYVSTVTVSPDGSLCASGGKDGAALLWDLSTGEQLF 230

HSP 2 Score: 50.0618 bits (118), Expect = 1.325e-34
Identity = 25/43 (58.14%), Postives = 31/43 (72.09%), Query Frame = 1 
Query:    4 HTKDVLSVAFSIDNRQIVSASRDRTIKLWNTARRVQVHRFKRE 132
            HTKDVL+VAFS D+R IVSA RD  I++WN A    +H F R+
Sbjct:  106 HTKDVLAVAFSPDDRLIVSAGRDNVIRVWNVAGEC-MHEFLRD 147
[Close]
BLAST of CF504464 vs. ExPASy Swiss-Prot
Match: GBLP_LEIIN (Guanine nucleotide-binding protein subunit beta-like protein OS=Leishmania infantum PE=2 SV=1)

HSP 1 Score: 115.931 bits (289), Expect = 1.325e-34
Identity = 50/84 (59.52%), Postives = 63/84 (75.00%), Query Frame = 3 
Query:  132 DAHTEWVSCVRFSPNTLQPTIVSASWDRTVKVWNLSNCKLRATLAGHTGYVNTVAVSPDGSLCASGGKDGVILLWDLAEGKRLY 383
            D H +WVS + FSP+   P +VS SWD T+KVWN++  K   TL GH+ YV+TV VSPDGSLCASGGKDG  LLWDL+ G++L+
Sbjct:  147 DGHEDWVSSICFSPSLEHPIVVSGSWDNTIKVWNVNGGKCERTLKGHSNYVSTVTVSPDGSLCASGGKDGAALLWDLSTGEQLF 230

HSP 2 Score: 50.0618 bits (118), Expect = 1.325e-34
Identity = 25/43 (58.14%), Postives = 31/43 (72.09%), Query Frame = 1 
Query:    4 HTKDVLSVAFSIDNRQIVSASRDRTIKLWNTARRVQVHRFKRE 132
            HTKDVL+VAFS D+R IVSA RD  I++WN A    +H F R+
Sbjct:  106 HTKDVLAVAFSPDDRLIVSAGRDNVIRVWNVAGEC-MHEFLRD 147
[Close]
BLAST of CF504464 vs. ExPASy Swiss-Prot
Match: GBLP_LEICH (Guanine nucleotide-binding protein subunit beta-like protein OS=Leishmania chagasi PE=2 SV=1)

HSP 1 Score: 115.931 bits (289), Expect = 1.325e-34
Identity = 50/84 (59.52%), Postives = 63/84 (75.00%), Query Frame = 3 
Query:  132 DAHTEWVSCVRFSPNTLQPTIVSASWDRTVKVWNLSNCKLRATLAGHTGYVNTVAVSPDGSLCASGGKDGVILLWDLAEGKRLY 383
            D H +WVS + FSP+   P +VS SWD T+KVWN++  K   TL GH+ YV+TV VSPDGSLCASGGKDG  LLWDL+ G++L+
Sbjct:  147 DGHEDWVSSICFSPSLEHPIVVSGSWDNTIKVWNVNGGKCERTLKGHSNYVSTVTVSPDGSLCASGGKDGAALLWDLSTGEQLF 230

HSP 2 Score: 50.0618 bits (118), Expect = 1.325e-34
Identity = 25/43 (58.14%), Postives = 31/43 (72.09%), Query Frame = 1 
Query:    4 HTKDVLSVAFSIDNRQIVSASRDRTIKLWNTARRVQVHRFKRE 132
            HTKDVL+VAFS D+R IVSA RD  I++WN A    +H F R+
Sbjct:  106 HTKDVLAVAFSPDDRLIVSAGRDNVIRVWNVAGEC-MHEFLRD 147
[Close]
BLAST of CF504464 vs. ExPASy Swiss-Prot
Match: GBLP_TRYBR (Guanine nucleotide-binding protein subunit beta-like protein OS=Trypanosoma brucei rhodesiense PE=2 SV=1)

HSP 1 Score: 110.538 bits (275), Expect = 1.097e-33
Identity = 51/82 (62.20%), Postives = 59/82 (71.95%), Query Frame = 3 
Query:  135 AHTEWVSCVRFSPNTLQPTIVSASWDRTVKVWNLSNCKLRATLAGHTGYVNTVAVSPDGSLCASGGKDGVILLWDLAEGKRL 380
            AHT+WVSCVRFSP+   P IVS  WD  VKVW+L+  +L   L GHT YV +V VSPDGSLCAS  KDGV  LWDL +G+ L
Sbjct:  150 AHTDWVSCVRFSPSLDAPVIVSGGWDNLVKVWDLATGRLVTDLKGHTNYVTSVTVSPDGSLCASSDKDGVARLWDLTKGEAL 231

HSP 2 Score: 52.373 bits (124), Expect = 1.097e-33
Identity = 23/31 (74.19%), Postives = 26/31 (83.87%), Query Frame = 1 
Query:    1 GHTKDVLSVAFSIDNRQIVSASRDRTIKLWN 93
            GHTKDVLSVAFS DNRQIVS  RD  +++WN
Sbjct:  107 GHTKDVLSVAFSPDNRQIVSGGRDNALRVWN 137
[Close]
BLAST of CF504464 vs. ExPASy Swiss-Prot
Match: GBLP_TRYBB (Guanine nucleotide-binding protein subunit beta-like protein OS=Trypanosoma brucei brucei PE=2 SV=1)

HSP 1 Score: 110.538 bits (275), Expect = 1.097e-33
Identity = 51/82 (62.20%), Postives = 59/82 (71.95%), Query Frame = 3 
Query:  135 AHTEWVSCVRFSPNTLQPTIVSASWDRTVKVWNLSNCKLRATLAGHTGYVNTVAVSPDGSLCASGGKDGVILLWDLAEGKRL 380
            AHT+WVSCVRFSP+   P IVS  WD  VKVW+L+  +L   L GHT YV +V VSPDGSLCAS  KDGV  LWDL +G+ L
Sbjct:  150 AHTDWVSCVRFSPSLDAPVIVSGGWDNLVKVWDLATGRLVTDLKGHTNYVTSVTVSPDGSLCASSDKDGVARLWDLTKGEAL 231

HSP 2 Score: 52.373 bits (124), Expect = 1.097e-33
Identity = 23/31 (74.19%), Postives = 26/31 (83.87%), Query Frame = 1 
Query:    1 GHTKDVLSVAFSIDNRQIVSASRDRTIKLWN 93
            GHTKDVLSVAFS DNRQIVS  RD  +++WN
Sbjct:  107 GHTKDVLSVAFSPDNRQIVSGGRDNALRVWN 137
[Close]
BLAST of CF504464 vs. ExPASy Swiss-Prot
Match: GBLP_YEAST (Guanine nucleotide-binding protein subunit beta-like protein OS=Saccharomyces cerevisiae GN=ASC1 PE=1 SV=4)

HSP 1 Score: 83.5741 bits (205), Expect = 2.105e-20
Identity = 41/86 (47.67%), Postives = 53/86 (61.63%), Query Frame = 3 
Query:  138 HTEWVSCVRFSPNTLQP----TIVSASWDRTVKVWNLSNCKLRATLAGHTGYVNTVAVSPDGSLCASGGKDGVILLWDLAEGKRLY 383
            H +WVS VR  PN        TI+SA  D+ VK WNL+  ++ A   GH   +NT+  SPDG+L AS GKDG I+LW+LA  K +Y
Sbjct:  147 HNDWVSQVRVVPNEKADDDSVTIISAGNDKMVKAWNLNQFQIEADFIGHNSNINTLTASPDGTLIASAGKDGEIMLWNLAAKKAMY 232

HSP 2 Score: 34.6538 bits (78), Expect = 2.105e-20
Identity = 15/30 (50.00%), Postives = 19/30 (63.33%), Query Frame = 1 
Query:    1 GHTKDVLSVAFSIDNRQIVSASRDRTIKLW 90
            GH  DV+SV        I+S SRD+TIK+W
Sbjct:  105 GHKSDVMSVDIDKKASMIISGSRDKTIKVW 134
The following BLAST results are available for this feature:
BLAST of CF504464 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 86
Match NameE-valueIdentityDescription
GBLP_NEUCR2.043e-4269.51Guanine nucleotide-binding protein subunit beta-li... [more]
GBLP_ORYSJ1.459e-3884.71Guanine nucleotide-binding protein subunit beta-li... [more]
GBLP_DICDI4.754e-3864.29Guanine nucleotide-binding protein subunit beta-li... [more]
GBLP_SCHPO9.422e-3662.20Guanine nucleotide-binding protein subunit beta-li... [more]
GBLP_LEIMA1.325e-3459.52Guanine nucleotide-binding protein subunit beta-li... [more]
GBLP_LEIIN1.325e-3459.52Guanine nucleotide-binding protein subunit beta-li... [more]
GBLP_LEICH1.325e-3459.52Guanine nucleotide-binding protein subunit beta-li... [more]
GBLP_TRYBR1.097e-3362.20Guanine nucleotide-binding protein subunit beta-li... [more]
GBLP_TRYBB1.097e-3362.20Guanine nucleotide-binding protein subunit beta-li... [more]
GBLP_YEAST2.105e-2047.67Guanine nucleotide-binding protein subunit beta-li... [more]

Pages

back to top
Properties
Property NameValue
Genbank descriptionUSDA-FP_119000-953 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-18_F05 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF504464 ID=CF504464; Name=CF504464; organism=Citrus sinensis; type=EST; length=384bp
GGCCACACTAAGGACGTTCTCTCCGTCGCCTTCTCGATCGACAACCGTCA
GATTGTATCGGCCTCTCGTGACCGTACGATCAAGTTGTGGAACACCGCTC
GGCGAGTGCAAGTACACCGATTCAAGAGGGAGACGCTCACACTGAGTGGG
TTTCTTGTGTTAGGTTCAGCCCCAACACGCTTCAGCCCACTATCGTTTCC
GCCTCGTGGGACCGTACGGTCAAAGTGTGGAATTTGAGCAACTGCAAGCT
TAGGGCTACTCTTGCCGGGCACACTGGATACGTGAACACCGTCGCAGTGT
CGCCGGATGGTTCGTTGTGTGCCAGTGGAGGGAAAGATGGTGTGATTCTG
TTGTGGGATTTGGCTGAGGGAAAAAGACTCTACT
back to top