CF504496

Overview
NameCF504496
Unique NameCF504496
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length587
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CF504496 vs. ExPASy Swiss-Prot
Match: PSMR_PYRKO (Proteasome-activating nucleotidase OS=Pyrococcus kodakaraensis GN=pan PE=3 SV=1)

HSP 1 Score: 78.5666 bits (192), Expect = 1.148e-17
Identity = 29/49 (59.18%), Postives = 44/49 (89.80%), Query Frame = 2 
Query:  353 EVGYDDVGGVRKQMAQIRELVELPLRHPQLFKSIGVKPPKGILLYGPPG 499
            +V Y+D+GG+ KQ+ ++RE +ELPL+HP+LF+ +G++PPKG+LLYGPPG
Sbjct:  134 KVTYNDIGGLEKQLQELREAIELPLKHPELFEQVGIEPPKGVLLYGPPG 182

HSP 2 Score: 30.4166 bits (67), Expect = 1.148e-17
Identity = 14/28 (50.00%), Postives = 19/28 (67.86%), Query Frame = 3 
Query:  504 GKTLIARAVANETGAFFFCINGPEIMSK 587
            GKTL+A+AVAN   A F  + G E++ K
Sbjct:  184 GKTLMAKAVANHVNATFIRVVGSELVRK 211
[Close]
BLAST of CF504496 vs. ExPASy Swiss-Prot
Match: PSMR_METKA (Proteasome-activating nucleotidase OS=Methanopyrus kandleri GN=pan PE=3 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 2.517e-17
Identity = 30/50 (60.00%), Postives = 44/50 (88.00%), Query Frame = 2 
Query:  353 EVGYDDVGGVRKQMAQIRELVELPLRHPQLFKSIGVKPPKGILLYGPPGS 502
            +V YDD+GG+ +Q+ +IRE+VE PL+ P+LF+ +GV+PPKG+LLYGPPG+
Sbjct:  175 DVSYDDIGGLDEQIREIREVVEKPLKEPELFEKVGVEPPKGVLLYGPPGT 224

HSP 2 Score: 31.5722 bits (70), Expect = 2.517e-17
Identity = 14/28 (50.00%), Postives = 19/28 (67.86%), Query Frame = 3 
Query:  504 GKTLIARAVANETGAFFFCINGPEIMSK 587
            GKTL+A+AVAN   A F  +  PE++ K
Sbjct:  225 GKTLLAKAVANHADATFIRLAAPELVQK 252
[Close]
BLAST of CF504496 vs. ExPASy Swiss-Prot
Match: PSMR_METB6 (Proteasome-activating nucleotidase OS=Methanoregula boonei (strain 6A8) GN=pan PE=3 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 7.188e-17
Identity = 28/47 (59.57%), Postives = 41/47 (87.23%), Query Frame = 2 
Query:  362 YDDVGGVRKQMAQIRELVELPLRHPQLFKSIGVKPPKGILLYGPPGS 502
            Y D+GG+++Q+ ++RE VELPL+ P+LF  IG++PPKG+LLYGPPG+
Sbjct:  177 YTDIGGLKEQINEVREAVELPLKRPELFTQIGIEPPKGVLLYGPPGT 223

HSP 2 Score: 33.4982 bits (75), Expect = 7.188e-17
Identity = 15/28 (53.57%), Postives = 21/28 (75.00%), Query Frame = 3 
Query:  504 GKTLIARAVANETGAFFFCINGPEIMSK 587
            GKTL+A+AVA+ET A F  + G E++ K
Sbjct:  224 GKTLLAKAVAHETNAHFMRVVGSELVQK 251
[Close]
BLAST of CF504496 vs. ExPASy Swiss-Prot
Match: PRS7B_ARATH (26S protease regulatory subunit 7 homolog B OS=Arabidopsis thaliana GN=RPT1B PE=3 SV=2)

HSP 1 Score: 68.9366 bits (167), Expect = 9.331e-17
Identity = 27/47 (57.45%), Postives = 38/47 (80.85%), Query Frame = 2 
Query:  362 YDDVGGVRKQMAQIRELVELPLRHPQLFKSIGVKPPKGILLYGPPGS 502
            Y D+GG ++Q+ +IRE+VELP+ HP+ F  +G+ PPKG+L YGPPGS
Sbjct:  204 YSDIGGCKEQIEKIREVVELPMLHPEKFVRLGIDPPKGVLCYGPPGS 250

HSP 2 Score: 36.965 bits (84), Expect = 9.331e-17
Identity = 17/28 (60.71%), Postives = 21/28 (75.00%), Query Frame = 3 
Query:  504 GKTLIARAVANETGAFFFCINGPEIMSK 587
            GKTL+ARAVAN TGA F  + G E++ K
Sbjct:  251 GKTLVARAVANRTGACFIRVVGSELVQK 278
[Close]
BLAST of CF504496 vs. ExPASy Swiss-Prot
Match: PSMR_ARCFU (Proteasome-activating nucleotidase OS=Archaeoglobus fulgidus GN=pan PE=1 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 1.217e-16
Identity = 30/50 (60.00%), Postives = 41/50 (82.00%), Query Frame = 2 
Query:  353 EVGYDDVGGVRKQMAQIRELVELPLRHPQLFKSIGVKPPKGILLYGPPGS 502
            EV Y+D+GG+  Q+ +IRE VELPL  P+LF  +G++PPKG+LLYGPPG+
Sbjct:  137 EVSYEDIGGLDVQIEEIREAVELPLLKPELFAEVGIEPPKGVLLYGPPGT 186

HSP 2 Score: 32.3426 bits (72), Expect = 1.217e-16
Identity = 15/28 (53.57%), Postives = 20/28 (71.43%), Query Frame = 3 
Query:  504 GKTLIARAVANETGAFFFCINGPEIMSK 587
            GKTL+A+AVAN+T A F  + G E + K
Sbjct:  187 GKTLLAKAVANQTRATFIRVVGSEFVQK 214
[Close]
BLAST of CF504496 vs. ExPASy Swiss-Prot
Match: PSMR_METHJ (Proteasome-activating nucleotidase OS=Methanospirillum hungatei (strain JF-1 / DSM 864) GN=pan PE=3 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 2.055e-16
Identity = 29/47 (61.70%), Postives = 39/47 (82.98%), Query Frame = 2 
Query:  362 YDDVGGVRKQMAQIRELVELPLRHPQLFKSIGVKPPKGILLYGPPGS 502
            Y+D+GG+ KQ+ +IRE VELP+  P LF+ IG+ PPKG+LLYGPPG+
Sbjct:  152 YEDIGGLEKQIMEIREAVELPMTRPDLFEKIGINPPKGVLLYGPPGT 198

HSP 2 Score: 31.5722 bits (70), Expect = 2.055e-16
Identity = 15/28 (53.57%), Postives = 20/28 (71.43%), Query Frame = 3 
Query:  504 GKTLIARAVANETGAFFFCINGPEIMSK 587
            GKTL+A+AVA+ET A F    G E++ K
Sbjct:  199 GKTLLAKAVAHETHAIFLHTVGSELVQK 226
[Close]
BLAST of CF504496 vs. ExPASy Swiss-Prot
Match: PSMR_METMJ (Proteasome-activating nucleotidase OS=Methanoculleus marisnigri (strain ATCC 35101 / DSM 1498 / JR1) GN=pan PE=3 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 2.055e-16
Identity = 29/50 (58.00%), Postives = 40/50 (80.00%), Query Frame = 2 
Query:  353 EVGYDDVGGVRKQMAQIRELVELPLRHPQLFKSIGVKPPKGILLYGPPGS 502
            E  Y+++GG+  Q+ +IRE VELPL  PQLF+ +G+ PPKG+LLYGPPG+
Sbjct:  149 EETYENIGGLEPQIEEIREAVELPLTKPQLFEKVGISPPKGVLLYGPPGT 198

HSP 2 Score: 33.4982 bits (75), Expect = 2.055e-16
Identity = 15/28 (53.57%), Postives = 21/28 (75.00%), Query Frame = 3 
Query:  504 GKTLIARAVANETGAFFFCINGPEIMSK 587
            GKTL+ARAVA++T A F  + G E++ K
Sbjct:  199 GKTLLARAVAHQTNAHFLRVVGSELVQK 226
[Close]
BLAST of CF504496 vs. ExPASy Swiss-Prot
Match: PRS8_DICDI (26S protease regulatory subunit 8 OS=Dictyostelium discoideum GN=psmC5 PE=1 SV=2)

HSP 1 Score: 72.7886 bits (177), Expect = 2.056e-16
Identity = 28/53 (52.83%), Postives = 45/53 (84.91%), Query Frame = 2 
Query:  344 RLDEVGYDDVGGVRKQMAQIRELVELPLRHPQLFKSIGVKPPKGILLYGPPGS 502
            ++ +  YD VGG+ KQ+ +I+E++ELP++HP+LF+S+G+  PKG+LLYGPPG+
Sbjct:  138 KIPDSTYDMVGGLDKQIKEIKEVIELPIKHPELFESLGIAQPKGVLLYGPPGT 190

HSP 2 Score: 31.9574 bits (71), Expect = 2.056e-16
Identity = 14/28 (50.00%), Postives = 20/28 (71.43%), Query Frame = 3 
Query:  504 GKTLIARAVANETGAFFFCINGPEIMSK 587
            GKTL+ARAVA+ T   F  ++G E++ K
Sbjct:  191 GKTLLARAVAHHTDCTFIRVSGSELVQK 218
[Close]
BLAST of CF504496 vs. ExPASy Swiss-Prot
Match: PSMR_METMP (Proteasome-activating nucleotidase OS=Methanococcus maripaludis GN=pan PE=3 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 3.473e-16
Identity = 26/50 (52.00%), Postives = 43/50 (86.00%), Query Frame = 2 
Query:  353 EVGYDDVGGVRKQMAQIRELVELPLRHPQLFKSIGVKPPKGILLYGPPGS 502
            ++ ++D+GG+  Q+  I+E+VELPL++P+LF+ +G+ PPKG+LLYGPPG+
Sbjct:  144 DISFEDIGGLNNQIRDIKEVVELPLKNPELFEKVGIVPPKGVLLYGPPGT 193

HSP 2 Score: 31.5722 bits (70), Expect = 3.473e-16
Identity = 15/28 (53.57%), Postives = 20/28 (71.43%), Query Frame = 3 
Query:  504 GKTLIARAVANETGAFFFCINGPEIMSK 587
            GKTL+A+AVA ET A F  + G E++ K
Sbjct:  194 GKTLLAKAVAYETNASFVRVVGSELVKK 221
[Close]
BLAST of CF504496 vs. ExPASy Swiss-Prot
Match: PSMR_METM7 (Proteasome-activating nucleotidase OS=Methanococcus maripaludis (strain C7 / ATCC BAA-1331) GN=pan PE=3 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 3.473e-16
Identity = 26/50 (52.00%), Postives = 43/50 (86.00%), Query Frame = 2 
Query:  353 EVGYDDVGGVRKQMAQIRELVELPLRHPQLFKSIGVKPPKGILLYGPPGS 502
            ++ ++D+GG+  Q+  I+E+VELPL++P+LF+ +G+ PPKG+LLYGPPG+
Sbjct:  144 DISFEDIGGLNNQIRDIKEVVELPLKNPELFEKVGIVPPKGVLLYGPPGT 193

HSP 2 Score: 31.5722 bits (70), Expect = 3.473e-16
Identity = 15/28 (53.57%), Postives = 20/28 (71.43%), Query Frame = 3 
Query:  504 GKTLIARAVANETGAFFFCINGPEIMSK 587
            GKTL+A+AVA ET A F  + G E++ K
Sbjct:  194 GKTLLAKAVAYETNASFVRVVGSELVKK 221
The following BLAST results are available for this feature:
BLAST of CF504496 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 155
Match NameE-valueIdentityDescription
PSMR_PYRKO1.148e-1759.18Proteasome-activating nucleotidase OS=Pyrococcus k... [more]
PSMR_METKA2.517e-1760.00Proteasome-activating nucleotidase OS=Methanopyrus... [more]
PSMR_METB67.188e-1759.57Proteasome-activating nucleotidase OS=Methanoregul... [more]
PRS7B_ARATH9.331e-1757.4526S protease regulatory subunit 7 homolog B OS=Ara... [more]
PSMR_ARCFU1.217e-1660.00Proteasome-activating nucleotidase OS=Archaeoglobu... [more]
PSMR_METHJ2.055e-1661.70Proteasome-activating nucleotidase OS=Methanospiri... [more]
PSMR_METMJ2.055e-1658.00Proteasome-activating nucleotidase OS=Methanoculle... [more]
PRS8_DICDI2.056e-1652.8326S protease regulatory subunit 8 OS=Dictyostelium... [more]
PSMR_METMP3.473e-1652.00Proteasome-activating nucleotidase OS=Methanococcu... [more]
PSMR_METM73.473e-1652.00Proteasome-activating nucleotidase OS=Methanococcu... [more]

Pages

back to top
Properties
Property NameValue
Genbank descriptionUSDA-FP_119000-985 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-19_A03 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF504496 ID=CF504496; Name=CF504496; organism=Citrus sinensis; type=EST; length=587bp
CCCCCGGGCTGCAGGGTCGTGAGGTCGAACTTGAGGGTTAGACTCGGAGA
TGTTGTTTCTGTGCACCAGTGCGCTGATGTCAAATACGGAAAGCGTGTGC
ACATATTACCTGTGGATGACACAATTGAAGGGGTTACCTGGAAATCTCTT
TGATGCATACTTGAAACCTTATTTCACGGAAGCTTATCGACCAGTGAGGA
AGGGTGATCTATTCCTTGTTAGAGGTGGAATGAGAAGTGTAGAGTTCAAG
GTTATTGAGACTGACCCTCCTGAATACTGTGTGGTTGCCCCTGATACTGA
GATCTTCTGTGAGGGAGAGCCAGTGAGAAGGGAAGATGAGAATCGATTAG
ATGAGGTTGGCTATGATGATGTTGGTGGGGTTAGAAAGCAGATGGCTCAG
ATTCGAGAATTAGTAGAACTTCCATTAAGGCACCCGCAGCTCTTTAAGTC
AATTGGTGTGAAGCCACCGAAAGGAATTTTGCTTTATGGACCTCCTGGAT
CTTGGGAAGACACTGATTGCCCGTGCTGTTGCAAATGAAACCGGTGCTTT
CTTCTTTTGTATAAATGGACCTGAGATCATGTCAAAA
back to top