CF504496

Overview
NameCF504496
Unique NameCF504496
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length587
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CF504496 vs. ExPASy Swiss-Prot
Match: PSMR_SULTO (Proteasome-activating nucleotidase OS=Sulfolobus tokodaii GN=pan PE=3 SV=2)

HSP 1 Score: 75.8702 bits (185), Expect = 5.871e-16
Identity = 30/49 (61.22%), Postives = 43/49 (87.76%), Query Frame = 2 
Query:  356 VGYDDVGGVRKQMAQIRELVELPLRHPQLFKSIGVKPPKGILLYGPPGS 502
            V Y D+GG+ +Q+ +IRE++ELPL++P+LFK IG+ PPKG+LLYGPPG+
Sbjct:  131 VHYSDIGGLNEQINEIREVIELPLKNPELFKEIGIDPPKGVLLYGPPGT 179

HSP 2 Score: 27.335 bits (59), Expect = 5.871e-16
Identity = 13/28 (46.43%), Postives = 17/28 (60.71%), Query Frame = 3 
Query:  504 GKTLIARAVANETGAFFFCINGPEIMSK 587
            GKTL+A+AVA E+ A F  +   E   K
Sbjct:  180 GKTLLAKAVATESNATFIQVVASEFAQK 207
[Close]
BLAST of CF504496 vs. ExPASy Swiss-Prot
Match: PRS4_SCHPO (26S protease regulatory subunit 4 homolog OS=Schizosaccharomyces pombe GN=mts2 PE=2 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 7.607e-16
Identity = 26/47 (55.32%), Postives = 39/47 (82.98%), Query Frame = 2 
Query:  362 YDDVGGVRKQMAQIRELVELPLRHPQLFKSIGVKPPKGILLYGPPGS 502
            Y D+GG+  Q+ +I+E VELPL HP+L++ +G+KPPKG++LYG PG+
Sbjct:  190 YADIGGLESQIQEIKEAVELPLTHPELYEEMGIKPPKGVILYGAPGT 236

HSP 2 Score: 33.8834 bits (76), Expect = 7.607e-16
Identity = 15/28 (53.57%), Postives = 21/28 (75.00%), Query Frame = 3 
Query:  504 GKTLIARAVANETGAFFFCINGPEIMSK 587
            GKTL+A+AVAN+T A F  + G E++ K
Sbjct:  237 GKTLLAKAVANQTSATFLRVVGSELIQK 264
[Close]
BLAST of CF504496 vs. ExPASy Swiss-Prot
Match: PRS4B_ARATH (26S proteasome regulatory subunit 4 homolog B OS=Arabidopsis thaliana GN=RPT2B PE=2 SV=1)

HSP 1 Score: 69.707 bits (169), Expect = 7.609e-16
Identity = 27/47 (57.45%), Postives = 39/47 (82.98%), Query Frame = 2 
Query:  362 YDDVGGVRKQMAQIRELVELPLRHPQLFKSIGVKPPKGILLYGPPGS 502
            Y D+GG+  Q+ +I+E VELPL HP+L++ IG+KPPKG++LYG PG+
Sbjct:  187 YADIGGLEAQIQEIKEAVELPLTHPELYEDIGIKPPKGVILYGEPGT 233

HSP 2 Score: 33.113 bits (74), Expect = 7.609e-16
Identity = 15/28 (53.57%), Postives = 20/28 (71.43%), Query Frame = 3 
Query:  504 GKTLIARAVANETGAFFFCINGPEIMSK 587
            GKTL+A+AVAN T A F  + G E++ K
Sbjct:  234 GKTLLAKAVANSTSATFLRVVGSELIQK 261
[Close]
BLAST of CF504496 vs. ExPASy Swiss-Prot
Match: PRS4A_ARATH (26S proteasome regulatory subunit 4 homolog A OS=Arabidopsis thaliana GN=RPT2A PE=2 SV=1)

HSP 1 Score: 69.707 bits (169), Expect = 7.609e-16
Identity = 27/47 (57.45%), Postives = 39/47 (82.98%), Query Frame = 2 
Query:  362 YDDVGGVRKQMAQIRELVELPLRHPQLFKSIGVKPPKGILLYGPPGS 502
            Y D+GG+  Q+ +I+E VELPL HP+L++ IG+KPPKG++LYG PG+
Sbjct:  187 YADIGGLEAQIQEIKEAVELPLTHPELYEDIGIKPPKGVILYGEPGT 233

HSP 2 Score: 33.113 bits (74), Expect = 7.609e-16
Identity = 15/28 (53.57%), Postives = 20/28 (71.43%), Query Frame = 3 
Query:  504 GKTLIARAVANETGAFFFCINGPEIMSK 587
            GKTL+A+AVAN T A F  + G E++ K
Sbjct:  234 GKTLLAKAVANSTSATFLRVVGSELIQK 261
[Close]
BLAST of CF504496 vs. ExPASy Swiss-Prot
Match: PRS4_RAT (26S protease regulatory subunit 4 OS=Rattus norvegicus GN=Psmc1 PE=2 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 7.610e-16
Identity = 26/47 (55.32%), Postives = 39/47 (82.98%), Query Frame = 2 
Query:  362 YDDVGGVRKQMAQIRELVELPLRHPQLFKSIGVKPPKGILLYGPPGS 502
            Y D+GG+  Q+ +I+E VELPL HP+ ++ +G+KPPKG++LYGPPG+
Sbjct:  184 YADIGGLDNQIQEIKESVELPLTHPEYYEEMGIKPPKGVILYGPPGT 230

HSP 2 Score: 33.8834 bits (76), Expect = 7.610e-16
Identity = 15/28 (53.57%), Postives = 21/28 (75.00%), Query Frame = 3 
Query:  504 GKTLIARAVANETGAFFFCINGPEIMSK 587
            GKTL+A+AVAN+T A F  + G E++ K
Sbjct:  231 GKTLLAKAVANQTSATFLRVVGSELIQK 258
[Close]
BLAST of CF504496 vs. ExPASy Swiss-Prot
Match: PRS4_MOUSE (26S protease regulatory subunit 4 OS=Mus musculus GN=Psmc1 PE=1 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 7.610e-16
Identity = 26/47 (55.32%), Postives = 39/47 (82.98%), Query Frame = 2 
Query:  362 YDDVGGVRKQMAQIRELVELPLRHPQLFKSIGVKPPKGILLYGPPGS 502
            Y D+GG+  Q+ +I+E VELPL HP+ ++ +G+KPPKG++LYGPPG+
Sbjct:  184 YADIGGLDNQIQEIKESVELPLTHPEYYEEMGIKPPKGVILYGPPGT 230

HSP 2 Score: 33.8834 bits (76), Expect = 7.610e-16
Identity = 15/28 (53.57%), Postives = 21/28 (75.00%), Query Frame = 3 
Query:  504 GKTLIARAVANETGAFFFCINGPEIMSK 587
            GKTL+A+AVAN+T A F  + G E++ K
Sbjct:  231 GKTLLAKAVANQTSATFLRVVGSELIQK 258
[Close]
BLAST of CF504496 vs. ExPASy Swiss-Prot
Match: PRS4_HUMAN (26S protease regulatory subunit 4 OS=Homo sapiens GN=PSMC1 PE=1 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 7.610e-16
Identity = 26/47 (55.32%), Postives = 39/47 (82.98%), Query Frame = 2 
Query:  362 YDDVGGVRKQMAQIRELVELPLRHPQLFKSIGVKPPKGILLYGPPGS 502
            Y D+GG+  Q+ +I+E VELPL HP+ ++ +G+KPPKG++LYGPPG+
Sbjct:  184 YADIGGLDNQIQEIKESVELPLTHPEYYEEMGIKPPKGVILYGPPGT 230

HSP 2 Score: 33.8834 bits (76), Expect = 7.610e-16
Identity = 15/28 (53.57%), Postives = 21/28 (75.00%), Query Frame = 3 
Query:  504 GKTLIARAVANETGAFFFCINGPEIMSK 587
            GKTL+A+AVAN+T A F  + G E++ K
Sbjct:  231 GKTLLAKAVANQTSATFLRVVGSELIQK 258
[Close]
BLAST of CF504496 vs. ExPASy Swiss-Prot
Match: PRS4_DICDI (26S protease regulatory subunit 4 homolog OS=Dictyostelium discoideum GN=psmC1 PE=1 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 7.610e-16
Identity = 25/47 (53.19%), Postives = 39/47 (82.98%), Query Frame = 2 
Query:  362 YDDVGGVRKQMAQIRELVELPLRHPQLFKSIGVKPPKGILLYGPPGS 502
            Y D+GG+  Q+ +++E +ELPL HP+L++ IG+KPPKG++LYG PG+
Sbjct:  183 YSDIGGLEAQVQEMKEAIELPLTHPELYEEIGIKPPKGVILYGEPGT 229

HSP 2 Score: 33.8834 bits (76), Expect = 7.610e-16
Identity = 15/28 (53.57%), Postives = 21/28 (75.00%), Query Frame = 3 
Query:  504 GKTLIARAVANETGAFFFCINGPEIMSK 587
            GKTL+A+AVAN+T A F  + G E++ K
Sbjct:  230 GKTLLAKAVANQTSATFLRVVGSELIQK 257
[Close]
BLAST of CF504496 vs. ExPASy Swiss-Prot
Match: PRS4_CHICK (26S protease regulatory subunit 4 OS=Gallus gallus GN=PSMC1 PE=2 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 7.610e-16
Identity = 26/47 (55.32%), Postives = 39/47 (82.98%), Query Frame = 2 
Query:  362 YDDVGGVRKQMAQIRELVELPLRHPQLFKSIGVKPPKGILLYGPPGS 502
            Y D+GG+  Q+ +I+E VELPL HP+ ++ +G+KPPKG++LYGPPG+
Sbjct:  184 YADIGGLDNQIQEIKESVELPLTHPEYYEEMGIKPPKGVILYGPPGT 230

HSP 2 Score: 33.8834 bits (76), Expect = 7.610e-16
Identity = 15/28 (53.57%), Postives = 21/28 (75.00%), Query Frame = 3 
Query:  504 GKTLIARAVANETGAFFFCINGPEIMSK 587
            GKTL+A+AVAN+T A F  + G E++ K
Sbjct:  231 GKTLLAKAVANQTSATFLRVVGSELIQK 258
[Close]
BLAST of CF504496 vs. ExPASy Swiss-Prot
Match: PSMR_METPE (Proteasome-activating nucleotidase OS=Methanosphaerula palustris (strain ATCC BAA-1556 / DSM 19958 / E1-9c) GN=pan PE=3 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 7.622e-16
Identity = 26/47 (55.32%), Postives = 42/47 (89.36%), Query Frame = 2 
Query:  362 YDDVGGVRKQMAQIRELVELPLRHPQLFKSIGVKPPKGILLYGPPGS 502
            Y+++GG+ KQ+ +IRE VELPL+ P++F+ +G++PPKG+LL+GPPG+
Sbjct:  152 YEEIGGLEKQINEIREAVELPLKKPEVFRKMGIEPPKGVLLHGPPGT 198

HSP 2 Score: 32.7278 bits (73), Expect = 7.622e-16
Identity = 15/28 (53.57%), Postives = 21/28 (75.00%), Query Frame = 3 
Query:  504 GKTLIARAVANETGAFFFCINGPEIMSK 587
            GKTL+ARAVA++T A F  + G E++ K
Sbjct:  199 GKTLLARAVAHQTEAHFLRVVGSELVQK 226
The following BLAST results are available for this feature:
BLAST of CF504496 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 155
Match NameE-valueIdentityDescription
PSMR_SULTO5.871e-1661.22Proteasome-activating nucleotidase OS=Sulfolobus t... [more]
PRS4_SCHPO7.607e-1655.3226S protease regulatory subunit 4 homolog OS=Schiz... [more]
PRS4B_ARATH7.609e-1657.4526S proteasome regulatory subunit 4 homolog B OS=A... [more]
PRS4A_ARATH7.609e-1657.4526S proteasome regulatory subunit 4 homolog A OS=A... [more]
PRS4_RAT7.610e-1655.3226S protease regulatory subunit 4 OS=Rattus norveg... [more]
PRS4_MOUSE7.610e-1655.3226S protease regulatory subunit 4 OS=Mus musculus ... [more]
PRS4_HUMAN7.610e-1655.3226S protease regulatory subunit 4 OS=Homo sapiens ... [more]
PRS4_DICDI7.610e-1653.1926S protease regulatory subunit 4 homolog OS=Dicty... [more]
PRS4_CHICK7.610e-1655.3226S protease regulatory subunit 4 OS=Gallus gallus... [more]
PSMR_METPE7.622e-1655.32Proteasome-activating nucleotidase OS=Methanosphae... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_119000-985 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-19_A03 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF504496 ID=CF504496; Name=CF504496; organism=Citrus sinensis; type=EST; length=587bp
CCCCCGGGCTGCAGGGTCGTGAGGTCGAACTTGAGGGTTAGACTCGGAGA
TGTTGTTTCTGTGCACCAGTGCGCTGATGTCAAATACGGAAAGCGTGTGC
ACATATTACCTGTGGATGACACAATTGAAGGGGTTACCTGGAAATCTCTT
TGATGCATACTTGAAACCTTATTTCACGGAAGCTTATCGACCAGTGAGGA
AGGGTGATCTATTCCTTGTTAGAGGTGGAATGAGAAGTGTAGAGTTCAAG
GTTATTGAGACTGACCCTCCTGAATACTGTGTGGTTGCCCCTGATACTGA
GATCTTCTGTGAGGGAGAGCCAGTGAGAAGGGAAGATGAGAATCGATTAG
ATGAGGTTGGCTATGATGATGTTGGTGGGGTTAGAAAGCAGATGGCTCAG
ATTCGAGAATTAGTAGAACTTCCATTAAGGCACCCGCAGCTCTTTAAGTC
AATTGGTGTGAAGCCACCGAAAGGAATTTTGCTTTATGGACCTCCTGGAT
CTTGGGAAGACACTGATTGCCCGTGCTGTTGCAAATGAAACCGGTGCTTT
CTTCTTTTGTATAAATGGACCTGAGATCATGTCAAAA
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