CF504496

Overview
NameCF504496
Unique NameCF504496
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length587
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CF504496 vs. ExPASy Swiss-Prot
Match: PRS8A_ARATH (26S protease regulatory subunit 8 homolog A OS=Arabidopsis thaliana GN=RPT6A PE=2 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 9.902e-16
Identity = 26/47 (55.32%), Postives = 42/47 (89.36%), Query Frame = 2 
Query:  362 YDDVGGVRKQMAQIRELVELPLRHPQLFKSIGVKPPKGILLYGPPGS 502
            YD +GG+ +Q+ +I+E++ELP++HP+LF+S+G+  PKG+LLYGPPG+
Sbjct:  160 YDMIGGLDQQIKEIKEVIELPIKHPELFESLGIAQPKGVLLYGPPGT 206

HSP 2 Score: 31.9574 bits (71), Expect = 9.902e-16
Identity = 14/28 (50.00%), Postives = 20/28 (71.43%), Query Frame = 3 
Query:  504 GKTLIARAVANETGAFFFCINGPEIMSK 587
            GKTL+ARAVA+ T   F  ++G E++ K
Sbjct:  207 GKTLLARAVAHHTDCTFIRVSGSELVQK 234
[Close]
BLAST of CF504496 vs. ExPASy Swiss-Prot
Match: PRS7_CAEEL (26S protease regulatory subunit 7 OS=Caenorhabditis elegans GN=rpt-1 PE=1 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 1.286e-15
Identity = 27/50 (54.00%), Postives = 41/50 (82.00%), Query Frame = 2 
Query:  353 EVGYDDVGGVRKQMAQIRELVELPLRHPQLFKSIGVKPPKGILLYGPPGS 502
            +V Y DVGG + Q+ ++RE+VE PL HP+ + ++G++PPKG+LLYGPPG+
Sbjct:  173 DVTYSDVGGCKDQIEKLREVVETPLLHPERYVNLGIEPPKGVLLYGPPGT 222

HSP 2 Score: 32.7278 bits (73), Expect = 1.286e-15
Identity = 16/28 (57.14%), Postives = 19/28 (67.86%), Query Frame = 3 
Query:  504 GKTLIARAVANETGAFFFCINGPEIMSK 587
            GKTL ARAVAN T A F  + G E++ K
Sbjct:  223 GKTLCARAVANRTDACFIRVIGSELVQK 250
[Close]
BLAST of CF504496 vs. ExPASy Swiss-Prot
Match: PRS6A_DICDI (26S protease regulatory subunit 6A homolog OS=Dictyostelium discoideum GN=psmC3 PE=1 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 1.287e-15
Identity = 41/103 (39.81%), Postives = 60/103 (58.25%), Query Frame = 2 
Query:  194 VRKGDLFLVRGGMRSVEFKVIETDPPEYCVVAPDTEIFCEGEPVRREDENRLDEVGYDDVGGVRKQMAQIRELVELPLRHPQLFKSIGVKPPKGILLYGPPGS 502
            +R GDL     G+    + +++T PPEY       EI          DE   +E  Y D+GG+ KQ+ ++ E V LP+ H + F+SIG+KPPKG+L+YGPPG+
Sbjct:  127 LRPGDLV----GINKDSYIILDTLPPEYDSRVKAMEI----------DEKPTEE--YSDIGGLDKQIQELVEAVVLPMTHKERFESIGIKPPKGVLMYGPPGT 213

HSP 2 Score: 27.7202 bits (60), Expect = 1.287e-15
Identity = 11/26 (42.31%), Postives = 19/26 (73.08%), Query Frame = 3 
Query:  504 GKTLIARAVANETGAFFFCINGPEIM 581
            GKTL+ARA A +T + +  + GP+++
Sbjct:  214 GKTLLARACAAQTNSTYLKLAGPQLV 239
[Close]
BLAST of CF504496 vs. ExPASy Swiss-Prot
Match: PSMR_METVS (Proteasome-activating nucleotidase OS=Methanococcus vannielii (strain SB / ATCC 35089 / DSM 1224) GN=pan PE=3 SV=1)

HSP 1 Score: 69.707 bits (169), Expect = 1.288e-15
Identity = 26/50 (52.00%), Postives = 42/50 (84.00%), Query Frame = 2 
Query:  353 EVGYDDVGGVRKQMAQIRELVELPLRHPQLFKSIGVKPPKGILLYGPPGS 502
            ++ + D+GG+  Q+  I+E+VELPL++P+LF+ +G+ PPKG+LLYGPPG+
Sbjct:  144 DILFGDIGGLNNQIRDIKEVVELPLKNPELFEKVGIVPPKGVLLYGPPGT 193

HSP 2 Score: 32.3426 bits (72), Expect = 1.288e-15
Identity = 15/28 (53.57%), Postives = 20/28 (71.43%), Query Frame = 3 
Query:  504 GKTLIARAVANETGAFFFCINGPEIMSK 587
            GKTL+A+AVA ET A F  + G E++ K
Sbjct:  194 GKTLLAKAVARETNASFVRVVGSELVKK 221
[Close]
BLAST of CF504496 vs. ExPASy Swiss-Prot
Match: PRS7_YEAST (26S protease regulatory subunit 7 homolog OS=Saccharomyces cerevisiae GN=RPT1 PE=1 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 1.668e-15
Identity = 29/50 (58.00%), Postives = 40/50 (80.00%), Query Frame = 2 
Query:  353 EVGYDDVGGVRKQMAQIRELVELPLRHPQLFKSIGVKPPKGILLYGPPGS 502
            +V Y DVGG + Q+ ++RE+VELPL  P+ F ++G+ PPKGILLYGPPG+
Sbjct:  205 DVTYSDVGGCKDQIEKLREVVELPLLSPERFATLGIDPPKGILLYGPPGT 254

HSP 2 Score: 32.7278 bits (73), Expect = 1.668e-15
Identity = 16/28 (57.14%), Postives = 19/28 (67.86%), Query Frame = 3 
Query:  504 GKTLIARAVANETGAFFFCINGPEIMSK 587
            GKTL ARAVAN T A F  + G E++ K
Sbjct:  255 GKTLCARAVANRTDATFIRVIGSELVQK 282
[Close]
BLAST of CF504496 vs. ExPASy Swiss-Prot
Match: PSMR_UNCMA (Proteasome-activating nucleotidase OS=Uncultured methanogenic archaeon RC-I GN=pan PE=3 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 1.672e-15
Identity = 26/50 (52.00%), Postives = 40/50 (80.00%), Query Frame = 2 
Query:  353 EVGYDDVGGVRKQMAQIRELVELPLRHPQLFKSIGVKPPKGILLYGPPGS 502
            E+ Y  +GG+  Q+ +++E VELPL  P+LF+ +G++PPKG+LLYGPPG+
Sbjct:  155 EIDYQSIGGLEAQINELKETVELPLLKPELFQKVGIEPPKGVLLYGPPGT 204

HSP 2 Score: 32.7278 bits (73), Expect = 1.672e-15
Identity = 16/28 (57.14%), Postives = 20/28 (71.43%), Query Frame = 3 
Query:  504 GKTLIARAVANETGAFFFCINGPEIMSK 587
            GKTL+A+AVAN T A F  I G E++ K
Sbjct:  205 GKTLLAKAVANRTKATFIRIIGSELVQK 232
[Close]
BLAST of CF504496 vs. ExPASy Swiss-Prot
Match: PSMR2_HALSA (Proteasome-activating nucleotidase 2 OS=Halobacterium salinarium GN=pan2 PE=3 SV=2)

HSP 1 Score: 70.8626 bits (172), Expect = 2.175e-15
Identity = 26/50 (52.00%), Postives = 42/50 (84.00%), Query Frame = 2 
Query:  353 EVGYDDVGGVRKQMAQIRELVELPLRHPQLFKSIGVKPPKGILLYGPPGS 502
            +VGY+D+GG+  Q+ ++RE VELP++ P LF+++G+ PP G+LL+GPPG+
Sbjct:  145 DVGYEDIGGLDDQLREVRETVELPMKDPGLFETVGINPPSGVLLHGPPGT 194

HSP 2 Score: 30.4166 bits (67), Expect = 2.175e-15
Identity = 14/28 (50.00%), Postives = 21/28 (75.00%), Query Frame = 3 
Query:  504 GKTLIARAVANETGAFFFCINGPEIMSK 587
            GKTL+A+AVA++T A F  + G E++ K
Sbjct:  195 GKTLMAKAVASQTDASFIKMAGSELVHK 222
[Close]
BLAST of CF504496 vs. ExPASy Swiss-Prot
Match: PRS4_ORYSJ (26S protease regulatory subunit 4 homolog OS=Oryza sativa subsp. japonica GN=TBP2 PE=2 SV=2)

HSP 1 Score: 67.781 bits (164), Expect = 2.820e-15
Identity = 26/47 (55.32%), Postives = 39/47 (82.98%), Query Frame = 2 
Query:  362 YDDVGGVRKQMAQIRELVELPLRHPQLFKSIGVKPPKGILLYGPPGS 502
            Y D+GG+  Q+ +I+E VELPL HP+L++ IG++PPKG++LYG PG+
Sbjct:  192 YADIGGLDAQIQEIKEAVELPLTHPELYEDIGIRPPKGVILYGEPGT 238

HSP 2 Score: 33.113 bits (74), Expect = 2.820e-15
Identity = 15/28 (53.57%), Postives = 20/28 (71.43%), Query Frame = 3 
Query:  504 GKTLIARAVANETGAFFFCINGPEIMSK 587
            GKTL+A+AVAN T A F  + G E++ K
Sbjct:  239 GKTLLAKAVANSTSATFLRVVGSELIQK 266
[Close]
BLAST of CF504496 vs. ExPASy Swiss-Prot
Match: PRS6B_ASPNG (26S protease regulatory subunit 6B homolog OS=Aspergillus niger GN=tbpA PE=3 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 2.823e-15
Identity = 31/55 (56.36%), Postives = 39/55 (70.91%), Query Frame = 2 
Query:  338 ENRLDEVGYDDVGGVRKQMAQIRELVELPLRHPQLFKSIGVKPPKGILLYGPPGS 502
            EN   +V Y DVGG+  Q  +IRE VELPL    L+K IG+ PP+G+LLYGPPG+
Sbjct:  157 ENEKPDVTYADVGGLDMQKQEIREAVELPLTQFDLYKQIGIDPPRGVLLYGPPGT 211

HSP 2 Score: 32.7278 bits (73), Expect = 2.823e-15
Identity = 14/28 (50.00%), Postives = 19/28 (67.86%), Query Frame = 3 
Query:  504 GKTLIARAVANETGAFFFCINGPEIMSK 587
            GKT++ +AVAN T A F  +NG E + K
Sbjct:  212 GKTMLVKAVANSTTASFIRVNGSEFVQK 239
[Close]
BLAST of CF504496 vs. ExPASy Swiss-Prot
Match: PRS8_RAT (26S protease regulatory subunit 8 OS=Rattus norvegicus GN=Psmc5 PE=1 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 2.826e-15
Identity = 25/47 (53.19%), Postives = 42/47 (89.36%), Query Frame = 2 
Query:  362 YDDVGGVRKQMAQIRELVELPLRHPQLFKSIGVKPPKGILLYGPPGS 502
            Y+ +GG+ KQ+ +I+E++ELP++HP+LF+++G+  PKG+LLYGPPG+
Sbjct:  148 YEMIGGLDKQIKEIKEVIELPVKHPELFEALGIAQPKGVLLYGPPGT 194

HSP 2 Score: 31.9574 bits (71), Expect = 2.826e-15
Identity = 14/28 (50.00%), Postives = 20/28 (71.43%), Query Frame = 3 
Query:  504 GKTLIARAVANETGAFFFCINGPEIMSK 587
            GKTL+ARAVA+ T   F  ++G E++ K
Sbjct:  195 GKTLLARAVAHHTDCTFIRVSGSELVQK 222
The following BLAST results are available for this feature:
BLAST of CF504496 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 155
Match NameE-valueIdentityDescription
PRS8A_ARATH9.902e-1655.3226S protease regulatory subunit 8 homolog A OS=Ara... [more]
PRS7_CAEEL1.286e-1554.0026S protease regulatory subunit 7 OS=Caenorhabditi... [more]
PRS6A_DICDI1.287e-1539.8126S protease regulatory subunit 6A homolog OS=Dict... [more]
PSMR_METVS1.288e-1552.00Proteasome-activating nucleotidase OS=Methanococcu... [more]
PRS7_YEAST1.668e-1558.0026S protease regulatory subunit 7 homolog OS=Sacch... [more]
PSMR_UNCMA1.672e-1552.00Proteasome-activating nucleotidase OS=Uncultured m... [more]
PSMR2_HALSA2.175e-1552.00Proteasome-activating nucleotidase 2 OS=Halobacter... [more]
PRS4_ORYSJ2.820e-1555.3226S protease regulatory subunit 4 homolog OS=Oryza... [more]
PRS6B_ASPNG2.823e-1556.3626S protease regulatory subunit 6B homolog OS=Aspe... [more]
PRS8_RAT2.826e-1553.1926S protease regulatory subunit 8 OS=Rattus norveg... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_119000-985 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-19_A03 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF504496 ID=CF504496; Name=CF504496; organism=Citrus sinensis; type=EST; length=587bp
CCCCCGGGCTGCAGGGTCGTGAGGTCGAACTTGAGGGTTAGACTCGGAGA
TGTTGTTTCTGTGCACCAGTGCGCTGATGTCAAATACGGAAAGCGTGTGC
ACATATTACCTGTGGATGACACAATTGAAGGGGTTACCTGGAAATCTCTT
TGATGCATACTTGAAACCTTATTTCACGGAAGCTTATCGACCAGTGAGGA
AGGGTGATCTATTCCTTGTTAGAGGTGGAATGAGAAGTGTAGAGTTCAAG
GTTATTGAGACTGACCCTCCTGAATACTGTGTGGTTGCCCCTGATACTGA
GATCTTCTGTGAGGGAGAGCCAGTGAGAAGGGAAGATGAGAATCGATTAG
ATGAGGTTGGCTATGATGATGTTGGTGGGGTTAGAAAGCAGATGGCTCAG
ATTCGAGAATTAGTAGAACTTCCATTAAGGCACCCGCAGCTCTTTAAGTC
AATTGGTGTGAAGCCACCGAAAGGAATTTTGCTTTATGGACCTCCTGGAT
CTTGGGAAGACACTGATTGCCCGTGCTGTTGCAAATGAAACCGGTGCTTT
CTTCTTTTGTATAAATGGACCTGAGATCATGTCAAAA
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