CF504563 | Citrus Genome Database

Overview


Name	CF504563
Unique Name	CF504563
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	497

Libraries


Library Name	Type
Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
Csv1_Contig242	contig	Csv1_Contig242:9..504.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

[Close]

BLAST of CF504563 vs. ExPASy Swiss-Prot
Match: RL10_YEAST (60S ribosomal protein L10 OS=Saccharomyces cerevisiae GN=RPL10 PE=1 SV=1)

HSP 1 Score: 145.591 bits (366), Expect = 1.183e-34
Identity = 75/107 (70.09%), Postives = 84/107 (78.50%), Query Frame = 1

Query:  160 RRKASTSS-PSAFTLVSWEKENVSSEALEAARIACNKYMAKYAGKDAFHLRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGTCARVAIGQVLLSVRCKDSN 477
            ++KA+    P    LVS E E +SSEALEAARI  NKYM   +G+DAFHLRVRVHPFHVLRINKMLSCAGADRLQ GMRGA+GKP G  ARV IGQ++ SVR KDSN
Sbjct:   38 KKKATVDEFPLCVHLVSNELEQLSSEALEAARICANKYMTTVSGRDAFHLRVRVHPFHVLRINKMLSCAGADRLQQGMRGAWGKPHGLAARVDIGQIIFSVRTKDSN 144

HSP 2 Score: 86.6557 bits (213), Expect = 6.524e-17
Identity = 39/51 (76.47%), Postives = 41/51 (80.39%), Query Frame = 3

Query:   45 MGRRPARCYRQIKNKPYPKSRFCRGVPDPKIRIYDVGMKKKGVDEFPFCVH 197
            M RRPARCYR  KNKPYPKSR+ R VPD KIRIYD+G KK  VDEFP CVH
Sbjct:    1 MARRPARCYRYQKNKPYPKSRYNRAVPDSKIRIYDLGKKKATVDEFPLCVH 51

[Close]

BLAST of CF504563 vs. ExPASy Swiss-Prot
Match: RL10B_SCHPO (60S ribosomal protein L10-B OS=Schizosaccharomyces pombe GN=rpl10b PE=2 SV=1)

HSP 1 Score: 145.206 bits (365), Expect = 1.546e-34
Identity = 76/114 (66.67%), Postives = 84/114 (73.68%), Query Frame = 1

Query:  154 G*RRKASTSSPSAFTLVSWEKENVSSEALEAARIACNKYMAKYAGKDAFHLRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGTCARVAIGQVLLSVRCKDSNSHHAQE 495
            G +R      P    LVS E E ++SEALEAARI  NKY+ K  GKD+FHLRVR HPFHV+RINKMLSCAGADRLQTGMR AFGKP G  ARV IGQVL+SVR KDS+   A E
Sbjct:   37 GRKRAGVDEFPLCIHLVSNEYEQITSEALEAARICANKYLVKIGGKDSFHLRVRAHPFHVVRINKMLSCAGADRLQTGMRHAFGKPNGLVARVNIGQVLMSVRTKDSSRATAIE 150

HSP 2 Score: 87.4261 bits (215), Expect = 3.825e-17
Identity = 37/51 (72.55%), Postives = 42/51 (82.35%), Query Frame = 3

Query:   45 MGRRPARCYRQIKNKPYPKSRFCRGVPDPKIRIYDVGMKKKGVDEFPFCVH 197
            M RRPARCYR  KNKPYPKSR+ R VPD KIRI+D+G K+ GVDEFP C+H
Sbjct:    1 MARRPARCYRYCKNKPYPKSRYNRAVPDSKIRIFDLGRKRAGVDEFPLCIH 51

[Close]

BLAST of CF504563 vs. ExPASy Swiss-Prot
Match: RL10A_SCHPO (60S ribosomal protein L10-A OS=Schizosaccharomyces pombe GN=rpl10a PE=2 SV=2)

HSP 1 Score: 144.821 bits (364), Expect = 2.019e-34
Identity = 75/114 (65.79%), Postives = 84/114 (73.68%), Query Frame = 1

Query:  154 G*RRKASTSSPSAFTLVSWEKENVSSEALEAARIACNKYMAKYAGKDAFHLRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGTCARVAIGQVLLSVRCKDSNSHHAQE 495
            G +R      P    LVS E E ++SEALEAARI  NKY+ K  GKD+FHLRVR HPFHV+RINKMLSCAGADRLQTGMR AFGKP G  ARV IGQ+L+SVR KDS+   A E
Sbjct:   37 GRKRAGVDEFPLCIHLVSNEYEQITSEALEAARICANKYLVKIGGKDSFHLRVRAHPFHVVRINKMLSCAGADRLQTGMRHAFGKPNGLVARVNIGQILMSVRTKDSSRATAIE 150

HSP 2 Score: 87.4261 bits (215), Expect = 3.825e-17
Identity = 37/51 (72.55%), Postives = 42/51 (82.35%), Query Frame = 3

Query:   45 MGRRPARCYRQIKNKPYPKSRFCRGVPDPKIRIYDVGMKKKGVDEFPFCVH 197
            M RRPARCYR  KNKPYPKSR+ R VPD KIRI+D+G K+ GVDEFP C+H
Sbjct:    1 MARRPARCYRYCKNKPYPKSRYNRAVPDSKIRIFDLGRKRAGVDEFPLCIH 51

[Close]

BLAST of CF504563 vs. ExPASy Swiss-Prot
Match: RL10_ENCCU (60S ribosomal protein L10 OS=Encephalitozoon cuniculi GN=RPL10 PE=1 SV=1)

HSP 1 Score: 143.28 bits (360), Expect = 5.874e-34
Identity = 72/106 (67.92%), Postives = 83/106 (78.30%), Query Frame = 1

Query:  160 RRKASTSS-PSAFTLVSWEKENVSSEALEAARIACNKYMAKYAGKDAFHLRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGTCARVAIGQVLLSVRCKDS 474
            RRKA     P     +S E+EN+S+EALEAARI  NKYM K+AGKD FHLR+RV+PFHVLRINKMLSCAGADRLQTGMRG+FGKP G  ARV   Q +LS+R K+S
Sbjct:   38 RRKAGVLEFPLLVNCISNERENLSAEALEAARICANKYMVKHAGKDNFHLRIRVYPFHVLRINKMLSCAGADRLQTGMRGSFGKPYGRAARVVFNQPILSIRTKES 143

HSP 2 Score: 73.9442 bits (180), Expect = 4.376e-13
Identity = 33/51 (64.71%), Postives = 39/51 (76.47%), Query Frame = 3

Query:   45 MGRRPARCYRQIKNKPYPKSRFCRGVPDPKIRIYDVGMKKKGVDEFPFCVH 197
            MGRRP RCYR +  K YPKSRF RGVPD KI+I+D+G +K GV EFP  V+
Sbjct:    1 MGRRPGRCYRYLSKKAYPKSRFNRGVPDSKIQIFDLGRRKAGVLEFPLLVN 51

[Close]

BLAST of CF504563 vs. ExPASy Swiss-Prot
Match: RL10_TOBAC (60S ribosomal protein L10 (Fragment) OS=Nicotiana tabacum GN=RPL10 PE=2 SV=1)

HSP 1 Score: 142.895 bits (359), Expect = 7.671e-34
Identity = 69/79 (87.34%), Postives = 71/79 (89.87%), Query Frame = 1

Query:  259 CNKYMAKYAGKDAFHLRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGTCARVAIGQVLLSVRCKDSNSHHAQE 495
            C    A  AGKDAFHLRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQG CARVAIGQVLLSVRCKD N++HAQE
Sbjct:    1 CGGCEAGSAGKDAFHLRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGVCARVAIGQVLLSVRCKDGNANHAQE 79

[Close]

BLAST of CF504563 vs. ExPASy Swiss-Prot
Match: RL10_EUGGR (60S ribosomal protein L10 OS=Euglena gracilis GN=RPL10 PE=2 SV=1)

HSP 1 Score: 142.51 bits (358), Expect = 1.002e-33
Identity = 70/105 (66.67%), Postives = 82/105 (78.10%), Query Frame = 1

Query:  154 G*RRKASTSSPSAFTLVSWEKENVSSEALEAARIACNKYMAKYAGKDAFHLRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGTCARVAIGQVLLSVRCK 468
            G R+  + + P  + L+S E+E + SEALEAARI  NKYM K AGK+A+H+RVRVHPFHV RINKMLSCAGADRLQ GMRGAFGKP G  ARV IGQ L+S+R K
Sbjct:   37 GMRKLNADTFPLTYHLISMEREQIGSEALEAARINANKYMIKNAGKEAYHIRVRVHPFHVCRINKMLSCAGADRLQQGMRGAFGKPNGLAARVRIGQPLMSIRVK 141

HSP 2 Score: 82.0333 bits (201), Expect = 1.607e-15
Identity = 35/51 (68.63%), Postives = 39/51 (76.47%), Query Frame = 3

Query:   45 MGRRPARCYRQIKNKPYPKSRFCRGVPDPKIRIYDVGMKKKGVDEFPFCVH 197
            MGRRPA+CYR  KNK YPKSR+CRGVP+ KIRIYD GM+K   D FP   H
Sbjct:    1 MGRRPAKCYRYCKNKAYPKSRYCRGVPEAKIRIYDCGMRKLNADTFPLTYH 51

[Close]

BLAST of CF504563 vs. ExPASy Swiss-Prot
Match: RL10_METKA (50S ribosomal protein L10e OS=Methanopyrus kandleri GN=rpl10e PE=3 SV=1)

HSP 1 Score: 105.145 bits (261), Expect = 2.521e-29
Identity = 52/100 (52.00%), Postives = 71/100 (71.00%), Query Frame = 1

Query:  196 TLVSWEKENVSSEALEAARIACNKYMAKYAGKDAFHLRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGTCARVAIGQVLLSVRCKDSNSHHAQE 495
            +LV+ E+  V+  ALEAAR+A N+Y+ K  G+  +HL++RV+P HVLR N + + AGADR+Q GMR AFGKP GT ARV  GQ +++VR    N  HA+E
Sbjct:   48 SLVARERAQVTHNALEAARVAANRYLTKTVGRQNYHLKIRVYPHHVLRENPLATGAGADRVQEGMRLAFGKPIGTAARVREGQRVVTVRIDPENFEHAKE 147

HSP 2 Score: 43.1282 bits (100), Expect = 2.521e-29
Identity = 18/37 (48.65%), Postives = 25/37 (67.57%), Query Frame = 3

Query:   45 MGRRPARCYRQIKNKPYPKSRFCRGVPDPKIRIYDVG 155
            M  RPAR YR+ K   Y +  + +GVPDPKI+ +D+G
Sbjct:    1 MVNRPARIYREWKGPAYTRREYIKGVPDPKIQQFDMG 37

[Close]

BLAST of CF504563 vs. ExPASy Swiss-Prot
Match: RL10_AERPE (50S ribosomal protein L10e OS=Aeropyrum pernix GN=rpl10e PE=3 SV=1)

HSP 1 Score: 98.9821 bits (245), Expect = 1.088e-26
Identity = 52/99 (52.53%), Postives = 65/99 (65.66%), Query Frame = 1

Query:  199 LVSWEKENVSSEALEAARIACNKYMAKYAGKDAFHLRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGTCARVAIGQVLLSVRCKDSNSHHAQE 495
            LV  E   +   ALEAARIA NKY+A   G   F+ R+RV+P HV+R NKM++ AGADRLQ GMR AFGKP GT ARV  G V+L V  +  +  + +E
Sbjct:   51 LVMLEAGQIRHNALEAARIAVNKYLATTIGDKNFYFRIRVYPHHVIRENKMMAFAGADRLQDGMRQAFGKPVGTAARVYPGTVVLEVWARKGDEENVKE 149

HSP 2 Score: 40.4318 bits (93), Expect = 1.088e-26
Identity = 16/40 (40.00%), Postives = 23/40 (57.50%), Query Frame = 3

Query:   45 MGRRPARCYRQIKNKPYPKSRFCRGVPDPKIRIYDVGMKK 164
            M  RPARCY      PY +  +  GVP PKI  +++G ++
Sbjct:    1 MPLRPARCYTHFSGPPYTRREYIHGVPPPKITRFEMGNRE 40

[Close]

BLAST of CF504563 vs. ExPASy Swiss-Prot
Match: RL10_STAMF (50S ribosomal protein L10e OS=Staphylothermus marinus (strain ATCC 43588 / DSM 3639 / F1) GN=rpl10e PE=3 SV=1)

HSP 1 Score: 100.138 bits (248), Expect = 1.417e-26
Identity = 50/99 (50.51%), Postives = 66/99 (66.67%), Query Frame = 1

Query:  199 LVSWEKENVSSEALEAARIACNKYMAKYAGKDAFHLRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGTCARVAIGQVLLSVRCKDSNSHHAQE 495
            LV  E   +   ALEAAR+  NKY++   G   + L++RV+P HVLR NKM++ AGADRLQ GMR AFGKP GT ARV  G +++ VR +  +  HA+E
Sbjct:   49 LVMIEAGQIRHNALEAARVMANKYLSSTVGDQNYFLKIRVYPHHVLRENKMMAFAGADRLQDGMRQAFGKPIGTAARVYPGTIVMEVRVRKEHVEHAKE 147

HSP 2 Score: 38.891 bits (89), Expect = 1.417e-26
Identity = 16/37 (43.24%), Postives = 21/37 (56.76%), Query Frame = 3

Query:   45 MGRRPARCYRQIKNKPYPKSRFCRGVPDPKIRIYDVG 155
            M  RPARCY      PY +  +  GVP PKI  +++G
Sbjct:    1 MPLRPARCYTHFSGPPYTRREYIPGVPQPKIVKFEMG 37

[Close]

BLAST of CF504563 vs. ExPASy Swiss-Prot
Match: RL10_HYPBU (50S ribosomal protein L10e OS=Hyperthermus butylicus (strain DSM 5456 / JCM 9403) GN=rpl10e PE=3 SV=1)

HSP 1 Score: 101.679 bits (252), Expect = 4.065e-26
Identity = 52/95 (54.74%), Postives = 65/95 (68.42%), Query Frame = 1

Query:  211 EKENVSSEALEAARIACNKYMAKYAGKDAFHLRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGTCARVAIGQVLLSVRCKDSNSHHAQE 495
            E+  +   ALEAAR+A +KY++   G   +  RVRV+P HVLR NKM++ AGADRLQ GMR AFGKP GT ARV  GQ +L VR K  +  HA+E
Sbjct:   58 ERGQIRHNALEAARVAVHKYLSTTIGDQNYFFRVRVYPHHVLRENKMMAFAGADRLQEGMRQAFGKPVGTAARVYPGQAVLEVRVKKEHLDHAKE 152

HSP 2 Score: 35.8094 bits (81), Expect = 4.065e-26
Identity = 16/42 (38.10%), Postives = 26/42 (61.90%), Query Frame = 3

Query:   45 MGRRPARCYRQIKNK-----PYPKSRFCRGVPDPKIRIYDVG 155
            M ++PARCY + ++K     PY +  +  GVP PKI+ + +G
Sbjct:    1 MPQKPARCYTKRRSKAFSGPPYTRKEYIHGVPPPKIQKFVMG 42

The following BLAST results are available for this feature:

BLAST of CF504563 vs. ExPASy Swiss-Prot

BLAST of CF504563 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 91


Match Name	E-value	Identity	Description
RL10_YEAST	1.183e-34	70.09	60S ribosomal protein L10 OS=Saccharomyces cerevis...	[more]
RL10B_SCHPO	1.546e-34	66.67	60S ribosomal protein L10-B OS=Schizosaccharomyces...	[more]
RL10A_SCHPO	2.019e-34	65.79	60S ribosomal protein L10-A OS=Schizosaccharomyces...	[more]
RL10_ENCCU	5.874e-34	67.92	60S ribosomal protein L10 OS=Encephalitozoon cunic...	[more]
RL10_TOBAC	7.671e-34	87.34	60S ribosomal protein L10 (Fragment) OS=Nicotiana ...	[more]
RL10_EUGGR	1.002e-33	66.67	60S ribosomal protein L10 OS=Euglena gracilis GN=R...	[more]
RL10_METKA	2.521e-29	52.00	50S ribosomal protein L10e OS=Methanopyrus kandler...	[more]
RL10_AERPE	1.088e-26	52.53	50S ribosomal protein L10e OS=Aeropyrum pernix GN=...	[more]
RL10_STAMF	1.417e-26	50.51	50S ribosomal protein L10e OS=Staphylothermus mari...	[more]
RL10_HYPBU	4.065e-26	54.74	50S ribosomal protein L10e OS=Hyperthermus butylic...	[more]

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Properties


Property Name	Value
Genbank description	USDA-FP_120000-052 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-19_G03 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CF504563 ID=CF504563; Name=CF504563; organism=Citrus sinensis; type=EST; length=497bp
GAGCAGAAAGAGAGAATAGCTGAGACCGAACACGAACGCTCGCCATGGGA
AGAAGACCGGCAAGGTGTTACCGCCAAATCAAGAACAAACCCTACCCAAA
GTCCCGCTTTTGCCGCGGTGTCCCCGATCCCAAGATCAGAATCTACGATG
TCGGGATGAAGAAGAAAGGCGTCGACGAGTTCCCCTTCTGCGTTCACCCT
CGTCAGTTGGGAGAAGGAGAACGTGTCGAGCGAGGCCTTGGAAGCTGCCC
GTATCGCTTGCAACAAGTACATGGCCAAGTACGCCGGCAAGGATGCTTTC
CATTTGCGAGTGAGGGTGCACCCCTTCCATGTTCTGCGTATTAACAAGAT
GCTTTCGTGTGCTGGGGCTGATAGGCTCCAGACTGGAATGAGGGGTGCTT
TTGGAAAGCCCCAAGGAACCTGTGCTCGTGTTGCTATCGGGCAGGTCTTG
CTTTCTGTCCGTTGCAAGGATAGTAACAGCCACCATGCTCAGGAGGC