CF504563

Overview
NameCF504563
Unique NameCF504563
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length497
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig242 contig Csv1_Contig242:9..504. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CF504563 vs. ExPASy Swiss-Prot
Match: RL10_METAC (50S ribosomal protein L10e OS=Methanosarcina acetivorans GN=rpl10e PE=3 SV=1)

HSP 1 Score: 87.8113 bits (216), Expect = 5.710e-21
Identity = 48/110 (43.64%), Postives = 68/110 (61.82%), Query Frame = 1 
Query:  166 KASTSSPSAFTLVSWEKENVSSEALEAARIACNKYMAKYAGKDAFHLRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGTCARVAIGQVLLSVRCKDSNSHHAQE 495
            KA+TS P   +L+  EK  +   ALEAARI  N+++    GK  F++++RV+P  V+R NK  + AGADR+ +GMR AFGK  GT ARV   Q + +V  +  N   A+E
Sbjct:   39 KANTSFPVKISLIVEEKCQIRHTALEAARITANRHLVADTGKMGFYMKLRVYPHEVIRENKQATGAGADRVSSGMRRAFGKNVGTAARVKPMQKIFTVAVEKQNFKAAKE 148

HSP 2 Score: 32.3426 bits (72), Expect = 5.710e-21
Identity = 12/39 (30.77%), Postives = 23/39 (58.97%), Query Frame = 3 
Query:   45 MGRRPARCYRQIKNKPYPKSRFCRGVPDPKIRIYDVGMK 161
            M R+P   YR ++ + + + ++  GVP  ++  YD+G K
Sbjct:    1 MVRKPGSMYRNVRQRSFTRRKYMGGVPGSQVIHYDMGDK 39
[Close]
BLAST of CF504563 vs. ExPASy Swiss-Prot
Match: RL10_METMP (50S ribosomal protein L10e OS=Methanococcus maripaludis GN=rpl10e PE=3 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 1.635e-20
Identity = 42/108 (38.89%), Postives = 61/108 (56.48%), Query Frame = 1 
Query:  172 STSSPSAFTLVSWEKENVSSEALEAARIACNKYMAKYAGKDAFHLRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGTCARVAIGQVLLSVRCKDSNSHHAQE 495
            S   P    LVS     +   ALE++RIA NKY+    G+  +   +RV+P  +LR NKM + AGADR+  GMR +FGK  GT A+V  GQ ++++       + A+E
Sbjct:   40 SAEFPVQVQLVSKSDILIRHNALESSRIAGNKYILSECGRTGYLFNIRVYPHEILRENKMAAGAGADRISDGMRLSFGKAVGTAAKVKKGQEIITIGVNPEKFYAAKE 147

HSP 2 Score: 41.5874 bits (96), Expect = 1.635e-20
Identity = 17/37 (45.95%), Postives = 23/37 (62.16%), Query Frame = 3 
Query:   45 MGRRPARCYRQIKNKPYPKSRFCRGVPDPKIRIYDVG 155
            M  RPARCYR I+ + Y +  + R VP PK+  Y +G
Sbjct:    1 MALRPARCYRTIERRSYTRKEYVRAVPQPKVVHYVMG 37
[Close]
BLAST of CF504563 vs. ExPASy Swiss-Prot
Match: RL10_UNCMA (50S ribosomal protein L10e OS=Uncultured methanogenic archaeon RC-I GN=rpl10e PE=3 SV=1)

HSP 1 Score: 85.8853 bits (211), Expect = 2.128e-20
Identity = 48/104 (46.15%), Postives = 63/104 (60.58%), Query Frame = 1 
Query:  184 PSAFTLVSWEKENVSSEALEAARIACNKYMAKYAGKDAFHLRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGTCARVAIGQVLLSVRCKDSNSHHAQE 495
            P A TLV  E   +  +ALEAARI+ N+Y+    GK  +  +VRV+P  VLR NK  + AGADR+  GMR AFGK  GT ARV  GQ + ++    +N   A+E
Sbjct:   44 PIAVTLVVNETCQIRHDALEAARISANRYLLNDVGKTNYRFKVRVYPHQVLRENKQATGAGADRVSDGMRRAFGKAVGTAARVYEGQGVFTIWVNRANFEKAKE 147

HSP 2 Score: 32.3426 bits (72), Expect = 2.128e-20
Identity = 12/37 (32.43%), Postives = 20/37 (54.05%), Query Frame = 3 
Query:   45 MGRRPARCYRQIKNKPYPKSRFCRGVPDPKIRIYDVG 155
            M R+P R Y++     Y +  +  GVP  K+  +D+G
Sbjct:    1 MARKPGRMYKKFSGPAYTRREYMGGVPGVKVAQFDMG 37
[Close]
BLAST of CF504563 vs. ExPASy Swiss-Prot
Match: RL10_METS5 (50S ribosomal protein L10e OS=Metallosphaera sedula (strain ATCC 51363 / DSM 5348) GN=rpl10e PE=3 SV=1)

HSP 1 Score: 77.411 bits (189), Expect = 2.767e-20
Identity = 41/93 (44.09%), Postives = 60/93 (64.52%), Query Frame = 1 
Query:  223 VSSEALEAARIACNKYMAKYAGKDA-FHLRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGTCARVA-IGQVLLSVRCKDSNSHHAQE 495
            +   ALEAAR+   K M    G +  F+L V  +P HV+R NKM++ AGADRLQ GMR +FGKP GT AR+  +G +++++R K  +   A++
Sbjct:   57 IRHNALEAARVIALKQMTSMVGNETDFYLYVTKYPHHVIRENKMMAFAGADRLQDGMRLSFGKPIGTAARITKLGDLIMAIRVKKEHLEFAKK 149

HSP 2 Score: 40.4318 bits (93), Expect = 2.767e-20
Identity = 18/44 (40.91%), Postives = 22/44 (50.00%), Query Frame = 3 
Query:   45 MGRRPARCYRQIKNKPYPKSRFCRGVPDPKIRIYDVGMKKKGVD 176
            M  RP RCYR      Y +  +  GVP PKI  + +G  KK  D
Sbjct:    1 MPLRPGRCYRHFSGPAYTRKEYIPGVPQPKITKFTMGDHKKDYD 44
[Close]
BLAST of CF504563 vs. ExPASy Swiss-Prot
Match: RL10_METM7 (50S ribosomal protein L10e OS=Methanococcus maripaludis (strain C7 / ATCC BAA-1331) GN=rpl10e PE=3 SV=1)

HSP 1 Score: 77.7962 bits (190), Expect = 3.599e-20
Identity = 41/109 (37.61%), Postives = 63/109 (57.80%), Query Frame = 1 
Query:  169 ASTSSPSAFTLVSWEKENVSSEALEAARIACNKYMAKYAGKDAFHLRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGTCARVAIGQVLLSVRCKDSNSHHAQE 495
            +S   P    L+S     +   ALE++RIA NKY+ +  G+  +   +RV+P  +LR NKM + AGADR+  GMR +FGK  GT A+V  GQ ++++       + A+E
Sbjct:   39 SSVEFPVEVQLISKSDILIRHNALESSRIAGNKYILRECGRTGYLFNIRVYPHEILRENKMAAGAGADRISDGMRLSFGKAVGTAAKVKKGQEIITIGVNPEKFYAAKE 147

HSP 2 Score: 39.6614 bits (91), Expect = 3.599e-20
Identity = 16/37 (43.24%), Postives = 22/37 (59.46%), Query Frame = 3 
Query:   45 MGRRPARCYRQIKNKPYPKSRFCRGVPDPKIRIYDVG 155
            M  RPARCYR  + + Y +  + R VP PK+  Y +G
Sbjct:    1 MALRPARCYRTTERRSYTRKEYVRAVPQPKVVHYVMG 37
[Close]
BLAST of CF504563 vs. ExPASy Swiss-Prot
Match: RL10_METM6 (50S ribosomal protein L10e OS=Methanococcus maripaludis (strain C6 / ATCC BAA-1332) GN=rpl10e PE=3 SV=1)

HSP 1 Score: 77.7962 bits (190), Expect = 3.599e-20
Identity = 41/109 (37.61%), Postives = 63/109 (57.80%), Query Frame = 1 
Query:  169 ASTSSPSAFTLVSWEKENVSSEALEAARIACNKYMAKYAGKDAFHLRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGTCARVAIGQVLLSVRCKDSNSHHAQE 495
            +S   P    L+S     +   ALE++RIA NKY+ +  G+  +   +RV+P  +LR NKM + AGADR+  GMR +FGK  GT A+V  GQ ++++       + A+E
Sbjct:   39 SSVEFPVEVQLISKSDILIRHNALESSRIAGNKYILRECGRTGYLFNIRVYPHEILRENKMAAGAGADRISDGMRLSFGKAVGTAAKVKKGQEIITIGVNPEKFYAAKE 147

HSP 2 Score: 39.6614 bits (91), Expect = 3.599e-20
Identity = 16/37 (43.24%), Postives = 22/37 (59.46%), Query Frame = 3 
Query:   45 MGRRPARCYRQIKNKPYPKSRFCRGVPDPKIRIYDVG 155
            M  RPARCYR  + + Y +  + R VP PK+  Y +G
Sbjct:    1 MALRPARCYRTTERRSYTRKEYVRAVPQPKVVHYVMG 37
[Close]
BLAST of CF504563 vs. ExPASy Swiss-Prot
Match: RL10_SULAC (50S ribosomal protein L10e OS=Sulfolobus acidocaldarius GN=rpl10e PE=3 SV=1)

HSP 1 Score: 82.8037 bits (203), Expect = 4.679e-20
Identity = 47/101 (46.53%), Postives = 67/101 (66.34%), Query Frame = 1 
Query:  199 LVSWEKENVSSEALEAARIACNKYMAK-YAGKDAFHLRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGTCARV-AIGQVLLSVRCKDSNSHHAQE 495
            L++ EK  +   ALEAAR+   KY++K  A +  F L V  +P HV+R NKM++ AGADRLQ GMR +FGKP GT AR+  +G++++ VR K  +   A+E
Sbjct:   49 LITTEKGQIRHNALEAARVIALKYLSKKLASEQNFALVVTKYPHHVIRENKMMAFAGADRLQDGMRLSFGKPIGTAARLEKLGELVMYVRVKKEHLEAAKE 149

HSP 2 Score: 34.2686 bits (77), Expect = 4.679e-20
Identity = 14/37 (37.84%), Postives = 20/37 (54.05%), Query Frame = 3 
Query:   45 MGRRPARCYRQIKNKPYPKSRFCRGVPDPKIRIYDVG 155
            M  RP RCYR+     Y +  +  G+P PKI  + +G
Sbjct:    1 MPLRPGRCYRKFSGPAYTRREYIPGIPMPKITKFVMG 37
[Close]
BLAST of CF504563 vs. ExPASy Swiss-Prot
Match: RL10_METBF (50S ribosomal protein L10e OS=Methanosarcina barkeri (strain Fusaro / DSM 804) GN=rpl10e PE=3 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 4.681e-20
Identity = 46/104 (44.23%), Postives = 66/104 (63.46%), Query Frame = 1 
Query:  166 KASTSSPSAFTLVSWEKENVSSEALEAARIACNKYMAKYAGKDAFHLRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGTCARVAIGQVLLSVRCKDSN 477
            KA+TS P   +L+  EK  +   ALEAARI  N+++ +  GK  +++++RV+P  VLR NK  + AGADR+ +GMR AFGK  GT ARV   Q + +V  +  N
Sbjct:   39 KANTSFPIKLSLLVEEKCQIRHTALEAARITANRHLIEDTGKMGYYMKLRVYPHEVLRENKQATGAGADRVSSGMRRAFGKNVGTAARVNPFQKIFTVAVEKQN 142

HSP 2 Score: 32.3426 bits (72), Expect = 4.681e-20
Identity = 12/39 (30.77%), Postives = 23/39 (58.97%), Query Frame = 3 
Query:   45 MGRRPARCYRQIKNKPYPKSRFCRGVPDPKIRIYDVGMK 161
            M R+P   YR ++ + + + ++  GVP  ++  YD+G K
Sbjct:    1 MVRKPGSMYRNVRQRSFTRRKYMGGVPGSQVIHYDMGDK 39
[Close]
BLAST of CF504563 vs. ExPASy Swiss-Prot
Match: RL10_METMJ (50S ribosomal protein L10e OS=Methanoculleus marisnigri (strain ATCC 35101 / DSM 1498 / JR1) GN=rpl10e PE=3 SV=1)

HSP 1 Score: 82.0333 bits (201), Expect = 4.683e-20
Identity = 45/92 (48.91%), Postives = 56/92 (60.87%), Query Frame = 1 
Query:  184 PSAFTLVSWEKENVSSEALEAARIACNKYMAKYAGKDAFHLRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGTCARVAIGQVLLSV 459
            P   ++V  E   +   ALEAARI  N+ + K  G+  FHL++R  P HVLR NK  + AGADR+  GMR AFGK  GT ARV  GQ + SV
Sbjct:   44 PVELSIVVDETCQIRHTALEAARIGINRQLQKEVGRANFHLKIRTFPHHVLRENKQATGAGADRVSEGMRLAFGKAVGTAARVEKGQKVFSV 135

HSP 2 Score: 35.039 bits (79), Expect = 4.683e-20
Identity = 15/37 (40.54%), Postives = 22/37 (59.46%), Query Frame = 3 
Query:   45 MGRRPARCYRQIKNKPYPKSRFCRGVPDPKIRIYDVG 155
            M R+PA+ YR +  K Y +  +  GVP  KI  +D+G
Sbjct:    1 MVRKPAKMYRNLAKKAYTRREYMGGVPGSKIVQFDMG 37
[Close]
BLAST of CF504563 vs. ExPASy Swiss-Prot
Match: RL10_METLZ (50S ribosomal protein L10e OS=Methanocorpusculum labreanum (strain ATCC 43576 / DSM 4855 / Z) GN=rpl10e PE=3 SV=1)

HSP 1 Score: 79.337 bits (194), Expect = 4.688e-20
Identity = 44/96 (45.83%), Postives = 59/96 (61.46%), Query Frame = 1 
Query:  172 STSSPSAFTLVSWEKENVSSEALEAARIACNKYMAKYAGKDAFHLRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGTCARVAIGQVLLSV 459
            S   P A  L + E   +   A+EAARI  N+ + K  G++ FHL++R +P HVLR +K  + AGADR+  GMR AFGK  GT ARVA  Q + +V
Sbjct:   40 SEDFPVAIHLEALEACQIRHTAMEAARINMNRRLMKTVGRNNFHLKIRAYPHHVLREHKQATGAGADRVSEGMRLAFGKAVGTAARVAPRQRVFTV 135

HSP 2 Score: 37.7354 bits (86), Expect = 4.688e-20
Identity = 18/51 (35.29%), Postives = 28/51 (54.90%), Query Frame = 3 
Query:   45 MGRRPARCYRQIKNKPYPKSRFCRGVPDPKIRIYDVGMKKKGVDEFPFCVH 197
            M R+PAR YR +  K Y +  +  GVP  K+  +D+G      ++FP  +H
Sbjct:    1 MVRKPARMYRNLAKKAYCRREYMGGVPGLKVVQFDMGNLS---EDFPVAIH 48
The following BLAST results are available for this feature:
BLAST of CF504563 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 91
Match NameE-valueIdentityDescription
RL10_METAC5.710e-2143.6450S ribosomal protein L10e OS=Methanosarcina aceti... [more]
RL10_METMP1.635e-2038.8950S ribosomal protein L10e OS=Methanococcus maripa... [more]
RL10_UNCMA2.128e-2046.1550S ribosomal protein L10e OS=Uncultured methanoge... [more]
RL10_METS52.767e-2044.0950S ribosomal protein L10e OS=Metallosphaera sedul... [more]
RL10_METM73.599e-2037.6150S ribosomal protein L10e OS=Methanococcus maripa... [more]
RL10_METM63.599e-2037.6150S ribosomal protein L10e OS=Methanococcus maripa... [more]
RL10_SULAC4.679e-2046.5350S ribosomal protein L10e OS=Sulfolobus acidocald... [more]
RL10_METBF4.681e-2044.2350S ribosomal protein L10e OS=Methanosarcina barke... [more]
RL10_METMJ4.683e-2048.9150S ribosomal protein L10e OS=Methanoculleus maris... [more]
RL10_METLZ4.688e-2045.8350S ribosomal protein L10e OS=Methanocorpusculum l... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_120000-052 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-19_G03 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF504563 ID=CF504563; Name=CF504563; organism=Citrus sinensis; type=EST; length=497bp
GAGCAGAAAGAGAGAATAGCTGAGACCGAACACGAACGCTCGCCATGGGA
AGAAGACCGGCAAGGTGTTACCGCCAAATCAAGAACAAACCCTACCCAAA
GTCCCGCTTTTGCCGCGGTGTCCCCGATCCCAAGATCAGAATCTACGATG
TCGGGATGAAGAAGAAAGGCGTCGACGAGTTCCCCTTCTGCGTTCACCCT
CGTCAGTTGGGAGAAGGAGAACGTGTCGAGCGAGGCCTTGGAAGCTGCCC
GTATCGCTTGCAACAAGTACATGGCCAAGTACGCCGGCAAGGATGCTTTC
CATTTGCGAGTGAGGGTGCACCCCTTCCATGTTCTGCGTATTAACAAGAT
GCTTTCGTGTGCTGGGGCTGATAGGCTCCAGACTGGAATGAGGGGTGCTT
TTGGAAAGCCCCAAGGAACCTGTGCTCGTGTTGCTATCGGGCAGGTCTTG
CTTTCTGTCCGTTGCAAGGATAGTAACAGCCACCATGCTCAGGAGGC
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