CF504563

Overview
NameCF504563
Unique NameCF504563
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length497
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig242 contig Csv1_Contig242:9..504. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CF504563 vs. ExPASy Swiss-Prot
Match: RL10_METM5 (50S ribosomal protein L10e OS=Methanococcus maripaludis (strain C5 / ATCC BAA-1333) GN=rpl10e PE=3 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 7.920e-20
Identity = 42/109 (38.53%), Postives = 62/109 (56.88%), Query Frame = 1 
Query:  169 ASTSSPSAFTLVSWEKENVSSEALEAARIACNKYMAKYAGKDAFHLRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGTCARVAIGQVLLSVRCKDSNSHHAQE 495
            +S   P    LVS     +   ALE++RIA NKY+    G+  +   +RV+P  +LR NKM + AGADR+  GMR +FGK  GT A+V  GQ ++++       + A+E
Sbjct:   39 SSVEFPVEVQLVSKSDILIRHNALESSRIAGNKYIMGECGRTGYLFNIRVYPHEILRENKMAAGAGADRISDGMRLSFGKAVGTAAKVKKGQEIITIGVNPERFYAAKE 147

HSP 2 Score: 39.6614 bits (91), Expect = 7.920e-20
Identity = 16/37 (43.24%), Postives = 22/37 (59.46%), Query Frame = 3 
Query:   45 MGRRPARCYRQIKNKPYPKSRFCRGVPDPKIRIYDVG 155
            M  RPARCYR  + + Y +  + R VP PK+  Y +G
Sbjct:    1 MALRPARCYRTTERRSYTRKEYVRAVPQPKVVHYVMG 37
[Close]
BLAST of CF504563 vs. ExPASy Swiss-Prot
Match: RL10_METBU (50S ribosomal protein L10e OS=Methanococcoides burtonii (strain DSM 6242) GN=rpl10e PE=3 SV=1)

HSP 1 Score: 81.6481 bits (200), Expect = 1.031e-19
Identity = 41/92 (44.57%), Postives = 59/92 (64.13%), Query Frame = 1 
Query:  184 PSAFTLVSWEKENVSSEALEAARIACNKYMAKYAGKDAFHLRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGTCARVAIGQVLLSV 459
            P   TL++ E+  +   ALEAARI  N+ M   AG+  FH+++RV+P  VLR NK  + AGADR+ +GMR A+GK   T ARV+  Q + ++
Sbjct:   44 PVKITLLADERCQIRHTALEAARITANRAMTTAAGRSGFHMKLRVYPHEVLRENKQATGAGADRVSSGMRAAYGKNVSTAARVSARQKIFTI 135

HSP 2 Score: 34.2686 bits (77), Expect = 1.031e-19
Identity = 13/39 (33.33%), Postives = 25/39 (64.10%), Query Frame = 3 
Query:   45 MGRRPARCYRQIKNKPYPKSRFCRGVPDPKIRIYDVGMK 161
            M R+PA  YR +K++ + + ++  GVP  ++  +D+G K
Sbjct:    1 MVRKPASMYRNVKSRSFTRRKYMGGVPGSQVIHFDMGNK 39
[Close]
BLAST of CF504563 vs. ExPASy Swiss-Prot
Match: RL10_THEON (50S ribosomal protein L10e OS=Thermococcus onnurineus (strain NA1) GN=rpl10e PE=3 SV=1)

HSP 1 Score: 73.559 bits (179), Expect = 1.095e-18
Identity = 39/91 (42.86%), Postives = 52/91 (57.14%), Query Frame = 1 
Query:  223 VSSEALEAARIACNKYMAKYAGKDAFHLRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGTCARVAIGQVLLSVRCKDSNSHHAQE 495
            +   ALEA RI  N+Y+ K  G+  +H ++RV+PF VLR N M +   ADR   GMR  FGKP G  ARV   Q +L+V   + +   A E
Sbjct:   57 IRQNALEAIRIQVNRYLQKNVGRSNYHFKIRVYPFQVLRENPMATGRKADRYGNGMRRPFGKPIGLAARVKKDQKILTVWVNEGHLKFALE 147

HSP 2 Score: 38.891 bits (89), Expect = 1.095e-18
Identity = 16/37 (43.24%), Postives = 22/37 (59.46%), Query Frame = 3 
Query:   45 MGRRPARCYRQIKNKPYPKSRFCRGVPDPKIRIYDVG 155
            MG RPA+  R +    Y +  + RG P PKI I+D+G
Sbjct:    1 MGLRPAKIDRDVDKPAYTRREYIRGAPGPKITIFDMG 37
[Close]
BLAST of CF504563 vs. ExPASy Swiss-Prot
Match: RL10_THESM (50S ribosomal protein L10e OS=Thermococcus sibiricus (strain MM 739 / DSM 12597) GN=rpl10e PE=3 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 1.425e-18
Identity = 39/84 (46.43%), Postives = 51/84 (60.71%), Query Frame = 1 
Query:  211 EKENVSSEALEAARIACNKYMAKYAGKDAFHLRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGTCARVAIGQVLLSVR 462
            E   +   ALEAAR   N+Y++K  G+  FH ++RV+PF VLR N M +   ADR   GMR  FGKP G  AR+   Q +L+VR
Sbjct:   53 EPVQIRQNALEAARTQLNRYLSKNVGRSNFHYKIRVYPFQVLRENPMATGRKADRYGNGMRRPFGKPIGLAARLKKDQKILTVR 136

HSP 2 Score: 36.1946 bits (82), Expect = 1.425e-18
Identity = 18/51 (35.29%), Postives = 26/51 (50.98%), Query Frame = 3 
Query:   45 MGRRPARCYRQIKNKPYPKSRFCRGVPDPKIRIYDVGMKKKGVDEFPFCVH 197
            M  RPA+  R +    Y +  + RG P P+I I+D+G    G  EF   +H
Sbjct:    1 MALRPAKIDRYVDKPAYTRREYIRGAPGPRITIFDMG-NPAGDFEFEVSLH 50
[Close]
BLAST of CF504563 vs. ExPASy Swiss-Prot
Match: RL10_PYRHO (50S ribosomal protein L10e OS=Pyrococcus horikoshii GN=rpl10e PE=3 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 1.425e-18
Identity = 38/84 (45.24%), Postives = 50/84 (59.52%), Query Frame = 1 
Query:  211 EKENVSSEALEAARIACNKYMAKYAGKDAFHLRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGTCARVAIGQVLLSVR 462
            E   +   ALEAAR   N+Y+ K  G+  +H ++RV+PF VLR N M +   ADR   GMR  FGKP G  AR+   Q +LS+R
Sbjct:   53 EPVQIRQNALEAARQQVNRYLQKNVGRSNYHFKIRVYPFQVLRENPMATGRKADRYGNGMRRPFGKPIGLAARLKRDQKILSIR 136

HSP 2 Score: 37.7354 bits (86), Expect = 1.425e-18
Identity = 19/51 (37.25%), Postives = 26/51 (50.98%), Query Frame = 3 
Query:   45 MGRRPARCYRQIKNKPYPKSRFCRGVPDPKIRIYDVGMKKKGVDEFPFCVH 197
            M  RPA+  R +    Y +  + RG P PKI I+D+G    G  EF   +H
Sbjct:    1 MALRPAKIDRYVDKPAYTRREYIRGAPGPKITIFDMG-NPAGDFEFEVALH 50
[Close]
BLAST of CF504563 vs. ExPASy Swiss-Prot
Match: RL10_SULTO (50S ribosomal protein L10e OS=Sulfolobus tokodaii GN=rpl10e PE=3 SV=2)

HSP 1 Score: 76.6406 bits (187), Expect = 1.426e-18
Identity = 44/101 (43.56%), Postives = 62/101 (61.39%), Query Frame = 1 
Query:  199 LVSWEKENVSSEALEAARIACNKYMAKYAGKDA-FHLRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGTCARV-AIGQVLLSVRCKDSNSHHAQE 495
            LV+ EK  +   ALEAAR+   K +    G D  F L V  +P HV+R NKM++ AGADRLQ GMR +FGKP GT AR+  +G +++  + K  +   A++
Sbjct:   49 LVALEKGQIRHNALEAARVLALKQLTNKTGSDQNFALIVLKYPHHVIRENKMMAFAGADRLQDGMRLSFGKPIGTAARIERLGDIIMIAKVKKEHLEIAKK 149

HSP 2 Score: 35.4242 bits (80), Expect = 1.426e-18
Identity = 15/37 (40.54%), Postives = 19/37 (51.35%), Query Frame = 3 
Query:   45 MGRRPARCYRQIKNKPYPKSRFCRGVPDPKIRIYDVG 155
            M  RP RCYR      Y +  +  GVP PKI  + +G
Sbjct:    1 MPLRPGRCYRHFSGPAYTRKEYIPGVPMPKITKFTMG 37
[Close]
BLAST of CF504563 vs. ExPASy Swiss-Prot
Match: RL10_THEGJ (50S ribosomal protein L10e OS=Thermococcus gammatolerans (strain DSM 15229 / JCM 11827 / EJ3) GN=rpl10e PE=3 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 1.852e-18
Identity = 37/79 (46.84%), Postives = 48/79 (60.76%), Query Frame = 1 
Query:  223 VSSEALEAARIACNKYMAKYAGKDAFHLRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGTCARVAIGQVLLSV 459
            +   ALEA RI  N+Y+ K  G+  +H ++RV+PF VLR N M +   ADR   GMR  FGKP G  ARV   Q +L+V
Sbjct:   57 IRQNALEAIRIQVNRYLQKNVGRSNYHFKIRVYPFQVLRENPMATGRKADRYGNGMRRPFGKPIGLAARVKKDQKILTV 135

HSP 2 Score: 38.891 bits (89), Expect = 1.852e-18
Identity = 16/37 (43.24%), Postives = 22/37 (59.46%), Query Frame = 3 
Query:   45 MGRRPARCYRQIKNKPYPKSRFCRGVPDPKIRIYDVG 155
            MG RPA+  R +    Y +  + RG P PKI I+D+G
Sbjct:    1 MGLRPAKIDRDVDKPAYTRREYIRGAPGPKITIFDMG 37
[Close]
BLAST of CF504563 vs. ExPASy Swiss-Prot
Match: RL10_PYRKO (50S ribosomal protein L10e OS=Pyrococcus kodakaraensis GN=rpl10e PE=3 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 1.852e-18
Identity = 37/79 (46.84%), Postives = 48/79 (60.76%), Query Frame = 1 
Query:  223 VSSEALEAARIACNKYMAKYAGKDAFHLRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGTCARVAIGQVLLSV 459
            +   ALEA RI  N+Y+ K  G+  +H ++RV+PF VLR N M +   ADR   GMR  FGKP G  ARV   Q +L+V
Sbjct:   57 IRQNALEAIRIQVNRYLQKNVGRSNYHFKIRVYPFQVLRENPMATGRKADRYGNGMRRPFGKPIGLAARVRKDQKILTV 135

HSP 2 Score: 38.891 bits (89), Expect = 1.852e-18
Identity = 16/37 (43.24%), Postives = 22/37 (59.46%), Query Frame = 3 
Query:   45 MGRRPARCYRQIKNKPYPKSRFCRGVPDPKIRIYDVG 155
            MG RPA+  R +    Y +  + RG P PKI I+D+G
Sbjct:    1 MGLRPAKIDRDVDKPAYTRREYIRGAPGPKITIFDMG 37
[Close]
BLAST of CF504563 vs. ExPASy Swiss-Prot
Match: RL10_PYRFU (50S ribosomal protein L10e OS=Pyrococcus furiosus GN=rpl10e PE=3 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 1.853e-18
Identity = 38/84 (45.24%), Postives = 50/84 (59.52%), Query Frame = 1 
Query:  211 EKENVSSEALEAARIACNKYMAKYAGKDAFHLRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGTCARVAIGQVLLSVR 462
            E   +   ALEAAR   N+Y+ K  G+  +H ++RV+PF VLR N M +   ADR   GMR  FGKP G  AR+   Q +LS+R
Sbjct:   53 EPVQIRQNALEAARQQVNRYLQKNVGRSNYHFKIRVYPFQVLRENPMATGRKADRYGNGMRRPFGKPIGLAARLKKDQKILSIR 136

HSP 2 Score: 37.3502 bits (85), Expect = 1.853e-18
Identity = 19/51 (37.25%), Postives = 26/51 (50.98%), Query Frame = 3 
Query:   45 MGRRPARCYRQIKNKPYPKSRFCRGVPDPKIRIYDVGMKKKGVDEFPFCVH 197
            M  RPA+  R +    Y +  + RG P PKI I+D+G    G  EF   +H
Sbjct:    1 MALRPAKIDRYVDKPAYTRREYIRGAPGPKITIFDMG-NPAGDFEFEVSLH 50
[Close]
BLAST of CF504563 vs. ExPASy Swiss-Prot
Match: RL10_PYRAB (50S ribosomal protein L10e OS=Pyrococcus abyssi GN=rpl10e PE=3 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 1.853e-18
Identity = 37/80 (46.25%), Postives = 49/80 (61.25%), Query Frame = 1 
Query:  223 VSSEALEAARIACNKYMAKYAGKDAFHLRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGTCARVAIGQVLLSVR 462
            +   ALEAAR   N+Y+ K  G+  +H ++RV+PF VLR N M +   ADR   GMR  FGKP G  AR+   Q +LS+R
Sbjct:   57 IRQNALEAARQQVNRYLQKNVGRSNYHFKIRVYPFQVLRENPMATGRKADRYGNGMRRPFGKPIGLAARLKKDQKVLSIR 136

HSP 2 Score: 38.5058 bits (88), Expect = 1.853e-18
Identity = 20/54 (37.04%), Postives = 27/54 (50.00%), Query Frame = 3 
Query:   45 MGRRPARCYRQIKNKPYPKSRFCRGVPDPKIRIYDVGMKKKGVDEFPFCVHPRQ 206
            M  RPA+  R +    Y +  + RG P PKI I+D+G    G  EF   +H  Q
Sbjct:    1 MALRPAKIDRYVDKPAYTRREYIRGAPGPKITIFDMG-NPAGDFEFEVALHVSQ 53
The following BLAST results are available for this feature:
BLAST of CF504563 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 91
Match NameE-valueIdentityDescription
RL10_METM57.920e-2038.5350S ribosomal protein L10e OS=Methanococcus maripa... [more]
RL10_METBU1.031e-1944.5750S ribosomal protein L10e OS=Methanococcoides bur... [more]
RL10_THEON1.095e-1842.8650S ribosomal protein L10e OS=Thermococcus onnurin... [more]
RL10_THESM1.425e-1846.4350S ribosomal protein L10e OS=Thermococcus sibiric... [more]
RL10_PYRHO1.425e-1845.2450S ribosomal protein L10e OS=Pyrococcus horikoshi... [more]
RL10_SULTO1.426e-1843.5650S ribosomal protein L10e OS=Sulfolobus tokodaii ... [more]
RL10_THEGJ1.852e-1846.8450S ribosomal protein L10e OS=Thermococcus gammato... [more]
RL10_PYRKO1.852e-1846.8450S ribosomal protein L10e OS=Pyrococcus kodakarae... [more]
RL10_PYRFU1.853e-1845.2450S ribosomal protein L10e OS=Pyrococcus furiosus ... [more]
RL10_PYRAB1.853e-1846.2550S ribosomal protein L10e OS=Pyrococcus abyssi GN... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_120000-052 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-19_G03 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF504563 ID=CF504563; Name=CF504563; organism=Citrus sinensis; type=EST; length=497bp
GAGCAGAAAGAGAGAATAGCTGAGACCGAACACGAACGCTCGCCATGGGA
AGAAGACCGGCAAGGTGTTACCGCCAAATCAAGAACAAACCCTACCCAAA
GTCCCGCTTTTGCCGCGGTGTCCCCGATCCCAAGATCAGAATCTACGATG
TCGGGATGAAGAAGAAAGGCGTCGACGAGTTCCCCTTCTGCGTTCACCCT
CGTCAGTTGGGAGAAGGAGAACGTGTCGAGCGAGGCCTTGGAAGCTGCCC
GTATCGCTTGCAACAAGTACATGGCCAAGTACGCCGGCAAGGATGCTTTC
CATTTGCGAGTGAGGGTGCACCCCTTCCATGTTCTGCGTATTAACAAGAT
GCTTTCGTGTGCTGGGGCTGATAGGCTCCAGACTGGAATGAGGGGTGCTT
TTGGAAAGCCCCAAGGAACCTGTGCTCGTGTTGCTATCGGGCAGGTCTTG
CTTTCTGTCCGTTGCAAGGATAGTAACAGCCACCATGCTCAGGAGGC
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