CF504598 | Citrus Genome Database

Overview


Name	CF504598
Unique Name	CF504598
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	383

Libraries


Library Name	Type
Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
Csv1_Contig10898	contig	Csv1_Contig10898:617..994.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

BLAST of CF504598 vs. ExPASy Swiss-Prot
Match: MAO1_SHEPC (NAD-dependent malic enzyme OS=Shewanella putrefaciens (strain CN-32 / ATCC BAA-453) GN=sfcA PE=3 SV=1)

HSP 1 Score: 62.3882 bits (150), Expect = 3.834e-14
Identity = 29/60 (48.33%), Postives = 41/60 (68.33%), Query Frame = 1

Query:    1 GIRPSACLPITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVL 180
            GI P+  LPIT+DVGT+N QLL+D  Y+G R  R  G+EYAE ++ FM AV   + + ++
Sbjct:  181 GISPAYTLPITLDVGTDNPQLLEDPMYMGWRHPRIGGEEYAEFIEAFMQAVHVRWPDTLI 240

HSP 2 Score: 34.6538 bits (78), Expect = 3.834e-14
Identity = 15/28 (53.57%), Postives = 18/28 (64.29%), Query Frame = 3

Query:  192 FEDFANHNAFELLAKYGTTHLVFNDDIQ 275
            FEDFA  NA  +L +Y   +  FNDDIQ
Sbjct:  242 FEDFAQKNAMPILERYKDRYCCFNDDIQ 269

BLAST of CF504598 vs. ExPASy Swiss-Prot
Match: MAO1_SHEON (NAD-dependent malic enzyme OS=Shewanella oneidensis GN=sfcA PE=3 SV=1)

HSP 1 Score: 62.3882 bits (150), Expect = 3.834e-14
Identity = 29/60 (48.33%), Postives = 41/60 (68.33%), Query Frame = 1

Query:    1 GIRPSACLPITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVL 180
            GI P+  LPIT+DVGT+N QLL+D  Y+G R  R  G+EYAE ++ FM AV   + + ++
Sbjct:  181 GISPAYTLPITLDVGTDNPQLLEDPMYMGWRHPRIGGEEYAEFIEAFMQAVHVRWPDTLI 240

HSP 2 Score: 34.6538 bits (78), Expect = 3.834e-14
Identity = 15/28 (53.57%), Postives = 18/28 (64.29%), Query Frame = 3

Query:  192 FEDFANHNAFELLAKYGTTHLVFNDDIQ 275
            FEDFA  NA  +L +Y   +  FNDDIQ
Sbjct:  242 FEDFAQKNAMPILERYKERYCCFNDDIQ 269

BLAST of CF504598 vs. ExPASy Swiss-Prot
Match: MAO1_SHEB9 (NAD-dependent malic enzyme OS=Shewanella baltica (strain OS195) GN=sfcA PE=3 SV=1)

HSP 1 Score: 62.3882 bits (150), Expect = 3.834e-14
Identity = 29/60 (48.33%), Postives = 41/60 (68.33%), Query Frame = 1

Query:    1 GIRPSACLPITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVL 180
            GI P+  LPIT+DVGT+N QLL+D  Y+G R  R  G+EYAE ++ FM AV   + + ++
Sbjct:  181 GISPAYTLPITLDVGTDNPQLLEDPMYMGWRHPRIGGEEYAEFIEAFMQAVHVRWPDTLI 240

HSP 2 Score: 34.6538 bits (78), Expect = 3.834e-14
Identity = 15/28 (53.57%), Postives = 18/28 (64.29%), Query Frame = 3

Query:  192 FEDFANHNAFELLAKYGTTHLVFNDDIQ 275
            FEDFA  NA  +L +Y   +  FNDDIQ
Sbjct:  242 FEDFAQKNAMPILERYKDRYCCFNDDIQ 269

BLAST of CF504598 vs. ExPASy Swiss-Prot
Match: MAO1_SHEB8 (NAD-dependent malic enzyme OS=Shewanella baltica (strain OS185) GN=sfcA PE=3 SV=1)

HSP 1 Score: 62.3882 bits (150), Expect = 3.834e-14
Identity = 29/60 (48.33%), Postives = 41/60 (68.33%), Query Frame = 1

Query:    1 GIRPSACLPITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVL 180
            GI P+  LPIT+DVGT+N QLL+D  Y+G R  R  G+EYAE ++ FM AV   + + ++
Sbjct:  181 GISPAYTLPITLDVGTDNPQLLEDPMYMGWRHPRIGGEEYAEFIEAFMQAVHVRWPDTLI 240

HSP 2 Score: 34.6538 bits (78), Expect = 3.834e-14
Identity = 15/28 (53.57%), Postives = 18/28 (64.29%), Query Frame = 3

Query:  192 FEDFANHNAFELLAKYGTTHLVFNDDIQ 275
            FEDFA  NA  +L +Y   +  FNDDIQ
Sbjct:  242 FEDFAQKNAMPILERYKDRYCCFNDDIQ 269

BLAST of CF504598 vs. ExPASy Swiss-Prot
Match: MAO1_SHEB5 (NAD-dependent malic enzyme OS=Shewanella baltica (strain OS155 / ATCC BAA-1091) GN=sfcA PE=3 SV=1)

HSP 1 Score: 62.3882 bits (150), Expect = 3.834e-14
Identity = 29/60 (48.33%), Postives = 41/60 (68.33%), Query Frame = 1

Query:    1 GIRPSACLPITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVL 180
            GI P+  LPIT+DVGT+N QLL+D  Y+G R  R  G+EYAE ++ FM AV   + + ++
Sbjct:  181 GISPAYTLPITLDVGTDNPQLLEDPMYMGWRHPRIGGEEYAEFIEAFMQAVHVRWPDTLI 240

HSP 2 Score: 34.6538 bits (78), Expect = 3.834e-14
Identity = 15/28 (53.57%), Postives = 18/28 (64.29%), Query Frame = 3

Query:  192 FEDFANHNAFELLAKYGTTHLVFNDDIQ 275
            FEDFA  NA  +L +Y   +  FNDDIQ
Sbjct:  242 FEDFAQKNAMPILERYKDRYCCFNDDIQ 269

BLAST of CF504598 vs. ExPASy Swiss-Prot
Match: MAO1_SHEB2 (NAD-dependent malic enzyme OS=Shewanella baltica (strain OS223) GN=sfcA PE=3 SV=1)

HSP 1 Score: 62.3882 bits (150), Expect = 3.834e-14
Identity = 29/60 (48.33%), Postives = 41/60 (68.33%), Query Frame = 1

Query:    1 GIRPSACLPITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVL 180
            GI P+  LPIT+DVGT+N QLL+D  Y+G R  R  G+EYAE ++ FM AV   + + ++
Sbjct:  181 GISPAYTLPITLDVGTDNPQLLEDPMYMGWRHPRIGGEEYAEFIEAFMQAVHVRWPDTLI 240

HSP 2 Score: 34.6538 bits (78), Expect = 3.834e-14
Identity = 15/28 (53.57%), Postives = 18/28 (64.29%), Query Frame = 3

Query:  192 FEDFANHNAFELLAKYGTTHLVFNDDIQ 275
            FEDFA  NA  +L +Y   +  FNDDIQ
Sbjct:  242 FEDFAQKNAMPILERYKDRYCCFNDDIQ 269

BLAST of CF504598 vs. ExPASy Swiss-Prot
Match: MAO1_BDEBA (NAD-dependent malic enzyme OS=Bdellovibrio bacteriovorus GN=sfcA PE=3 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 4.979e-14
Identity = 29/60 (48.33%), Postives = 42/60 (70.00%), Query Frame = 1

Query:    1 GIRPSACLPITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVL 180
            GI P+  LP+ +DVGTNN+ LL D  Y+G RQ R TG+EY + +D F+ AVK+ + + +L
Sbjct:  184 GISPAYTLPVVLDVGTNNQALLNDPLYMGWRQPRITGKEYEDFVDAFVQAVKRRWPDVLL 243

HSP 2 Score: 31.5722 bits (70), Expect = 4.979e-14
Identity = 15/28 (53.57%), Postives = 17/28 (60.71%), Query Frame = 3

Query:  192 FEDFANHNAFELLAKYGTTHLVFNDDIQ 275
            FEDFA  NA  +L +Y      FNDDIQ
Sbjct:  245 FEDFAQKNASPILKRYRDEICSFNDDIQ 272

BLAST of CF504598 vs. ExPASy Swiss-Prot
Match: MAO1_SHEPA (NAD-dependent malic enzyme OS=Shewanella pealeana (strain ATCC 700345 / ANG-SQ1) GN=sfcA PE=3 SV=1)

HSP 1 Score: 61.2326 bits (147), Expect = 4.980e-14
Identity = 27/60 (45.00%), Postives = 41/60 (68.33%), Query Frame = 1

Query:    1 GIRPSACLPITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVL 180
            GI P+  LPIT+DVGT+N  LL+D  Y+G+R  R  G+EY E ++ FM AV + + + ++
Sbjct:  181 GISPAYTLPITLDVGTDNPHLLEDPMYMGMRSPRIGGEEYTEFVEAFMQAVHRRWPDALI 240

HSP 2 Score: 35.4242 bits (80), Expect = 4.980e-14
Identity = 16/28 (57.14%), Postives = 18/28 (64.29%), Query Frame = 3

Query:  192 FEDFANHNAFELLAKYGTTHLVFNDDIQ 275
            FEDFA  NA  LL +Y   +  FNDDIQ
Sbjct:  242 FEDFAQKNAMPLLERYKDQYCCFNDDIQ 269

BLAST of CF504598 vs. ExPASy Swiss-Prot
Match: MAOX_DICDI (NADP-dependent malic enzyme OS=Dictyostelium discoideum GN=malA PE=2 SV=1)

HSP 1 Score: 51.6026 bits (122), Expect = 5.934e-14
Identity = 25/62 (40.32%), Postives = 39/62 (62.90%), Query Frame = 1

Query:    1 GIRPSACLPITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVLIQ 186
            G  P+  LP+ +D GTN ++ L+D++Y+G R  R    EY  L+DEF++A   N   KV++Q
Sbjct:  172 GFCPTRTLPVIIDSGTNTKKYLEDKYYLGERHPRIPDSEYYPLVDEFLAAA-FNKWPKVIVQ 232

HSP 2 Score: 35.039 bits (79), Expect = 5.934e-14
Identity = 15/28 (53.57%), Postives = 19/28 (67.86%), Query Frame = 3

Query:  192 FEDFANHNAFELLAKYGTTHLVFNDDIQ 275
            FED +N + F LL +Y   +L FNDDIQ
Sbjct:  233 FEDISNDHCFNLLDEYRNKYLCFNDDIQ 260

HSP 3 Score: 29.261 bits (64), Expect = 5.934e-14
Identity = 11/18 (61.11%), Postives = 14/18 (77.78%), Query Frame = 2

Query:  329 LAEHRFLFLGAGEAGTGI 382
            + EHR +FLGAG AG G+
Sbjct:  281 IKEHRMVFLGAGSAGIGV 298

BLAST of CF504598 vs. ExPASy Swiss-Prot
Match: MAO1_VIBFM (NAD-dependent malic enzyme OS=Vibrio fischeri (strain MJ11) GN=sfcA PE=3 SV=1)

HSP 1 Score: 62.3882 bits (150), Expect = 6.469e-14
Identity = 28/60 (46.67%), Postives = 41/60 (68.33%), Query Frame = 1

Query:    1 GIRPSACLPITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVL 180
            GI P+  LPI +DVGTNN Q L D  Y+G R  R TG EYA+ +++F+ AV++ + E ++
Sbjct:  181 GISPAHTLPIVLDVGTNNPQRLADPMYMGWRHPRVTGDEYADFVEDFIQAVQRRWPEALV 240

HSP 2 Score: 33.8834 bits (76), Expect = 6.469e-14
Identity = 16/28 (57.14%), Postives = 17/28 (60.71%), Query Frame = 3

Query:  192 FEDFANHNAFELLAKYGTTHLVFNDDIQ 275
            FEDFA  NA  LL +Y      FNDDIQ
Sbjct:  242 FEDFAQKNAMPLLERYKNRICCFNDDIQ 269

The following BLAST results are available for this feature:

BLAST of CF504598 vs. ExPASy Swiss-Prot

BLAST of CF504598 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 137


Match Name	E-value	Identity	Description
MAO1_SHEPC	3.834e-14	48.33	NAD-dependent malic enzyme OS=Shewanella putrefaci...	[more]
MAO1_SHEON	3.834e-14	48.33	NAD-dependent malic enzyme OS=Shewanella oneidensi...	[more]
MAO1_SHEB9	3.834e-14	48.33	NAD-dependent malic enzyme OS=Shewanella baltica (...	[more]
MAO1_SHEB8	3.834e-14	48.33	NAD-dependent malic enzyme OS=Shewanella baltica (...	[more]
MAO1_SHEB5	3.834e-14	48.33	NAD-dependent malic enzyme OS=Shewanella baltica (...	[more]
MAO1_SHEB2	3.834e-14	48.33	NAD-dependent malic enzyme OS=Shewanella baltica (...	[more]
MAO1_BDEBA	4.979e-14	48.33	NAD-dependent malic enzyme OS=Bdellovibrio bacteri...	[more]
MAO1_SHEPA	4.980e-14	45.00	NAD-dependent malic enzyme OS=Shewanella pealeana ...	[more]
MAOX_DICDI	5.934e-14	40.32	NADP-dependent malic enzyme OS=Dictyostelium disco...	[more]
MAO1_VIBFM	6.469e-14	46.67	NAD-dependent malic enzyme OS=Vibrio fischeri (str...	[more]

Pages

Properties


Property Name	Value
Genbank description	USDA-FP_120000-087 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-1_B08 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CF504598 ID=CF504598; Name=CF504598; organism=Citrus sinensis; type=EST; length=383bp
GGCATTCGTCCCTCAGCATGTTTGCCCATAACCGTTGATGTGGGTACAAA
CAATGAGCAATTGTTGAAGGATGAATTCTACATTGGTCTACGACAGAGGA
GGGCAACCGGACAGGAATATGCTGAACTTCTAGATGAATTCATGAGTGCT
GTCAAGCAAAATTATGGAGAAAAAGTTCTTATACAGGTTAGTTTGAGGAT
TTCGCAAACCACAATGCTTTTGAGTTGCTTGCAAAATATGGCACAACTCA
TCTTGTCTTCAATGATGATATACAGCGTCTGTTGTTCTTGCTGGGGTAGT
TGCAGCACTGAAGTTGATTGGTGGCACACTGGCCGAGCATAGATTTTTGT
TTCTTGGAGCTGGGGAGGCTGGGACTGGTATAG