CF504605

Overview
NameCF504605
Unique NameCF504605
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length431
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CF504605 vs. ExPASy Swiss-Prot
Match: PCKA_BACCN (Phosphoenolpyruvate carboxykinase [ATP] OS=Bacillus cereus subsp. cytotoxis (strain NVH 391-98) GN=pckA PE=3 SV=1)

HSP 1 Score: 132.494 bits (332), Expect = 1.156e-40
Identity = 63/102 (61.76%), Postives = 78/102 (76.47%), Query Frame = 3 
Query:    3 IDLSREKEPDIWNAIKFGAVLENVVFDEHTREVDYSDKSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALL 308
            I+LS EKEP I++AIKFG+VLENV+ D+ TR  DYSD ++TENTRAAYPI+ I N  +P V  HP  +I L  DA GVLPP+SKL+  Q MYHF+SGYT+ L
Sbjct:  275 INLSHEKEPQIFDAIKFGSVLENVIVDDKTRIADYSDTALTENTRAAYPIDAIDNIVLPSVAGHPNTIIFLTADASGVLPPISKLSKEQAMYHFLSGYTSKL 376

HSP 2 Score: 53.9138 bits (128), Expect = 1.156e-40
Identity = 23/41 (56.10%), Postives = 30/41 (73.17%), Query Frame = 2 
Query:  305 AGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKH 427
            AGTE G+  P ATFS CFG+ F+ L  ++YA ML EK++KH
Sbjct:  377 AGTERGVTSPQATFSTCFGSPFLPLDASRYAEMLGEKIEKH 417
[Close]
BLAST of CF504605 vs. ExPASy Swiss-Prot
Match: PCKA1_MOOTA (Phosphoenolpyruvate carboxykinase [ATP] 1 OS=Moorella thermoacetica (strain ATCC 39073) GN=pckA1 PE=3 SV=1)

HSP 1 Score: 134.42 bits (337), Expect = 3.330e-40
Identity = 61/102 (59.80%), Postives = 78/102 (76.47%), Query Frame = 3 
Query:    3 IDLSREKEPDIWNAIKFGAVLENVVFDEHTREVDYSDKSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALL 308
            I LS E EP IWNAI+FG+VLENV+ D   R +DY   ++TENTRAAYP+++IPNA IP VG HP+ V+ L  DAFGV+PP++KL   Q MY+F+SGYT+ L
Sbjct:  273 IKLSAEHEPQIWNAIRFGSVLENVMVDPDCRIIDYDSDALTENTRAAYPVDFIPNAVIPGVGGHPQTVVFLTADAFGVMPPIAKLTREQAMYYFLSGYTSKL 374

HSP 2 Score: 50.447 bits (119), Expect = 3.330e-40
Identity = 22/41 (53.66%), Postives = 28/41 (68.29%), Query Frame = 2 
Query:  305 AGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKH 427
            AGTE G+ EP ATFS CFGA F+   P  YA +L E++ +H
Sbjct:  375 AGTERGVTEPKATFSTCFGAPFLPRSPMVYANLLGERIARH 415
[Close]
BLAST of CF504605 vs. ExPASy Swiss-Prot
Match: PCKA_LYSSC (Phosphoenolpyruvate carboxykinase [ATP] OS=Lysinibacillus sphaericus (strain C3-41) GN=pckA PE=3 SV=1)

HSP 1 Score: 134.035 bits (336), Expect = 4.339e-40
Identity = 64/102 (62.75%), Postives = 76/102 (74.51%), Query Frame = 3 
Query:    3 IDLSREKEPDIWNAIKFGAVLENVVFDEHTREVDYSDKSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALL 308
            I+LS EKEP+I+NAI+FG+VLENV  D  TR  DY D S+TENTR AYPI+YI N   P V  HPK +I L  DAFGVLPP+SKL   Q MYHF+SG+T+ L
Sbjct:  275 INLSAEKEPEIYNAIRFGSVLENVAVDPETRICDYDDGSLTENTRVAYPIQYIENIVDPSVAGHPKTIIFLTADAFGVLPPISKLTKEQAMYHFLSGFTSKL 376

HSP 2 Score: 50.447 bits (119), Expect = 4.339e-40
Identity = 22/42 (52.38%), Postives = 28/42 (66.67%), Query Frame = 2 
Query:  305 AGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKHG 430
            AGTE G+ EP   FS CFG+ F+ L  T YA ML +K+ +HG
Sbjct:  377 AGTERGVTEPEPVFSTCFGSPFLPLPATVYAEMLGQKIDEHG 418
[Close]
BLAST of CF504605 vs. ExPASy Swiss-Prot
Match: PCKA_NITSB (Phosphoenolpyruvate carboxykinase [ATP] OS=Nitratiruptor sp. (strain SB155-2) GN=pckA PE=3 SV=1)

HSP 1 Score: 117.857 bits (294), Expect = 5.654e-40
Identity = 62/100 (62.00%), Postives = 72/100 (72.00%), Query Frame = 3 
Query:    3 IDLSREKEPDIWNAIKFGAVLENVVFDEHTREVDYSDKSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTA 302
            I L  EKEPDI+ AIK  A+LENVV  E+  EVD+ D S TENTR +YPI +I   K    GPHPKN+I L+ DAFGVLPPVSKL   Q MY+F+SGYTA
Sbjct:  273 IGLDPEKEPDIYAAIKRDALLENVVVKENG-EVDFEDSSKTENTRVSYPIYHICKHKEDLQGPHPKNIIFLSADAFGVLPPVSKLTKEQAMYYFLSGYTA 371

HSP 2 Score: 66.2402 bits (160), Expect = 5.654e-40
Identity = 30/42 (71.43%), Postives = 33/42 (78.57%), Query Frame = 2 
Query:  305 AGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKHG 430
            AGTE GI EP ATFSACFG AF+ LHPT YA +L EK+ KHG
Sbjct:  374 AGTERGITEPVATFSACFGEAFLPLHPTVYAKLLGEKIDKHG 415
[Close]
BLAST of CF504605 vs. ExPASy Swiss-Prot
Match: PCKA_ALKOO (Phosphoenolpyruvate carboxykinase [ATP] OS=Alkaliphilus oremlandii (strain OhILAs) GN=pckA PE=3 SV=1)

HSP 1 Score: 126.716 bits (317), Expect = 9.597e-40
Identity = 59/102 (57.84%), Postives = 74/102 (72.55%), Query Frame = 3 
Query:    3 IDLSREKEPDIWNAIKFGAVLENVVFDEHTREVDYSDKSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALL 308
            I+L+ E+EP IWNAIK GA+LEN+V DE+T  +DY D   TENTRAAYP+EYI +A +   G  P  +I L  DA GVLPP++KL   Q MYHF+SGYT+ L
Sbjct:  273 INLTEEQEPQIWNAIKHGAILENIVIDENTGHIDYDDDRYTENTRAAYPVEYIDDAVLEGKGGIPNTIIFLTADATGVLPPIAKLTKEQAMYHFMSGYTSKL 374

HSP 2 Score: 56.6102 bits (135), Expect = 9.597e-40
Identity = 25/41 (60.98%), Postives = 31/41 (75.61%), Query Frame = 2 
Query:  305 AGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKH 427
            AGTE GI EPTATFS CFG  F++L P  YA +L EK++K+
Sbjct:  375 AGTERGIVEPTATFSTCFGGPFMLLKPQTYAKLLGEKIEKY 415
[Close]
BLAST of CF504605 vs. ExPASy Swiss-Prot
Match: PCKA_BACWK (Phosphoenolpyruvate carboxykinase [ATP] OS=Bacillus weihenstephanensis (strain KBAB4) GN=pckA PE=3 SV=1)

HSP 1 Score: 128.642 bits (322), Expect = 1.629e-39
Identity = 61/102 (59.80%), Postives = 76/102 (74.51%), Query Frame = 3 
Query:    3 IDLSREKEPDIWNAIKFGAVLENVVFDEHTREVDYSDKSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALL 308
            ++LS EKEP I++AIKFG+VLENVV D  TR  DY+D ++TENTRAAYP+  I N  +P V  HP  +I L  DA GVLPP+SKL+  Q MYHF+SGYT+ L
Sbjct:  275 VNLSHEKEPQIFDAIKFGSVLENVVIDGQTRIADYNDTTLTENTRAAYPMHAIDNIVLPSVAGHPNTIIFLTADASGVLPPISKLSKEQAMYHFLSGYTSKL 376

HSP 2 Score: 53.9138 bits (128), Expect = 1.629e-39
Identity = 23/41 (56.10%), Postives = 30/41 (73.17%), Query Frame = 2 
Query:  305 AGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKH 427
            AGTE G+  P ATFS CFG+ F+ L  ++YA ML EK++KH
Sbjct:  377 AGTERGVTSPQATFSTCFGSPFLPLDASRYAEMLGEKIEKH 417
[Close]
BLAST of CF504605 vs. ExPASy Swiss-Prot
Match: PCKA_OCEIH (Phosphoenolpyruvate carboxykinase [ATP] OS=Oceanobacillus iheyensis GN=pckA PE=3 SV=1)

HSP 1 Score: 127.872 bits (320), Expect = 2.765e-39
Identity = 60/102 (58.82%), Postives = 75/102 (73.53%), Query Frame = 3 
Query:    3 IDLSREKEPDIWNAIKFGAVLENVVFDEHTREVDYSDKSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALL 308
            I+LS EKEP I+NAI++G VLENV+ ++++RE DY D S+TENTRAAYP+E I N   P V  HP  +I L  DA G LPP+SKL   Q MYHF+SGYT+ L
Sbjct:  273 INLSEEKEPQIYNAIRYGTVLENVILNDNSREPDYDDTSLTENTRAAYPLENIDNIINPSVAGHPNTIIFLTADASGTLPPISKLTKEQAMYHFLSGYTSKL 374

HSP 2 Score: 53.9138 bits (128), Expect = 2.765e-39
Identity = 24/38 (63.16%), Postives = 30/38 (78.95%), Query Frame = 2 
Query:  305 AGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKM 418
            AGTE G+ EP ATFSACFG+ F+ L P+KYA ML +K+
Sbjct:  375 AGTERGVTEPLATFSACFGSPFLPLAPSKYAEMLGKKI 412
[Close]
BLAST of CF504605 vs. ExPASy Swiss-Prot
Match: PCKA_BACC2 (Phosphoenolpyruvate carboxykinase [ATP] OS=Bacillus cereus (strain G9842) GN=pckA PE=3 SV=1)

HSP 1 Score: 127.102 bits (318), Expect = 4.693e-39
Identity = 59/102 (57.84%), Postives = 76/102 (74.51%), Query Frame = 3 
Query:    3 IDLSREKEPDIWNAIKFGAVLENVVFDEHTREVDYSDKSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALL 308
            ++LS EKEP I++AIKFG+VLENV+ +  TR  DY+D ++TENTRAAYP+  I N  +P V  HP  +I L  DA GVLPP+SKL+  Q MYHF+SGYT+ L
Sbjct:  275 VNLSHEKEPQIFDAIKFGSVLENVIINNQTRIADYNDTTLTENTRAAYPMHAIDNIVLPSVAGHPNTIIFLTADASGVLPPISKLSKEQAMYHFLSGYTSKL 376

HSP 2 Score: 53.9138 bits (128), Expect = 4.693e-39
Identity = 23/41 (56.10%), Postives = 30/41 (73.17%), Query Frame = 2 
Query:  305 AGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKH 427
            AGTE G+  P ATFS CFG+ F+ L  ++YA ML EK++KH
Sbjct:  377 AGTERGVTSPQATFSTCFGSPFLPLDASRYAEMLGEKIEKH 417
[Close]
BLAST of CF504605 vs. ExPASy Swiss-Prot
Match: PCKA_BACLD (Phosphoenolpyruvate carboxykinase [ATP] OS=Bacillus licheniformis (strain DSM 13 / ATCC 14580) GN=pckA PE=3 SV=1)

HSP 1 Score: 132.109 bits (331), Expect = 4.694e-39
Identity = 63/102 (61.76%), Postives = 75/102 (73.53%), Query Frame = 3 
Query:    3 IDLSREKEPDIWNAIKFGAVLENVVFDEHTREVDYSDKSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALL 308
            I+LS EKEP I+NAI+FG+VLENVV D  T E DYSD   TENTRAAYPI+ I N   P +  HP+ ++ L  DAFGVLPP+SKL   Q MYHF+SGYT+ L
Sbjct:  275 INLSEEKEPQIYNAIRFGSVLENVVVDSKTGEPDYSDSFYTENTRAAYPIDAIDNIVKPSIAAHPQTIVFLTADAFGVLPPISKLTKEQAMYHFLSGYTSKL 376

HSP 2 Score: 48.9062 bits (115), Expect = 4.694e-39
Identity = 23/42 (54.76%), Postives = 27/42 (64.29%), Query Frame = 2 
Query:  305 AGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKHG 430
            AGTE GI  P ATFS CFG+ F+ L    YA ML  K+ +HG
Sbjct:  377 AGTERGITTPEATFSTCFGSPFLPLPAHVYAEMLGRKIDEHG 418
[Close]
BLAST of CF504605 vs. ExPASy Swiss-Prot
Match: PCKA_BACSK (Phosphoenolpyruvate carboxykinase [ATP] OS=Bacillus clausii (strain KSM-K16) GN=pckA PE=3 SV=1)

HSP 1 Score: 130.568 bits (327), Expect = 4.694e-39
Identity = 63/102 (61.76%), Postives = 75/102 (73.53%), Query Frame = 3 
Query:    3 IDLSREKEPDIWNAIKFGAVLENVVFDEHTREVDYSDKSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALL 308
            I LS+E EP IW+AI FGAVLENVV D +T + D+    +TENTRAAYP+E IPNA +P V   PK +I L  DAFGVLPP+SKL   Q MYHF+SGYT+ L
Sbjct:  274 IGLSKESEPQIWDAIAFGAVLENVVVDPNTGKPDFKSGELTENTRAAYPLEAIPNALLPSVAGPPKAIIFLTADAFGVLPPISKLTKEQAMYHFLSGYTSKL 375

HSP 2 Score: 50.447 bits (119), Expect = 4.694e-39
Identity = 22/39 (56.41%), Postives = 28/39 (71.79%), Query Frame = 2 
Query:  305 AGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQ 421
            AGTE GI +P ATFS CFGA F+   P +YA ML +K++
Sbjct:  376 AGTERGITQPEATFSTCFGAPFLPRKPEEYATMLGDKLE 414
The following BLAST results are available for this feature:
BLAST of CF504605 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 284
Match NameE-valueIdentityDescription
PCKA_BACCN1.156e-4061.76Phosphoenolpyruvate carboxykinase [ATP] OS=Bacillu... [more]
PCKA1_MOOTA3.330e-4059.80Phosphoenolpyruvate carboxykinase [ATP] 1 OS=Moore... [more]
PCKA_LYSSC4.339e-4062.75Phosphoenolpyruvate carboxykinase [ATP] OS=Lysinib... [more]
PCKA_NITSB5.654e-4062.00Phosphoenolpyruvate carboxykinase [ATP] OS=Nitrati... [more]
PCKA_ALKOO9.597e-4057.84Phosphoenolpyruvate carboxykinase [ATP] OS=Alkalip... [more]
PCKA_BACWK1.629e-3959.80Phosphoenolpyruvate carboxykinase [ATP] OS=Bacillu... [more]
PCKA_OCEIH2.765e-3958.82Phosphoenolpyruvate carboxykinase [ATP] OS=Oceanob... [more]
PCKA_BACC24.693e-3957.84Phosphoenolpyruvate carboxykinase [ATP] OS=Bacillu... [more]
PCKA_BACLD4.694e-3961.76Phosphoenolpyruvate carboxykinase [ATP] OS=Bacillu... [more]
PCKA_BACSK4.694e-3961.76Phosphoenolpyruvate carboxykinase [ATP] OS=Bacillu... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_120000-094 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-1_C03 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF504605 ID=CF504605; Name=CF504605; organism=Citrus sinensis; type=EST; length=431bp
GCATTGATCTCTCAAGGGAGAAGGAGCCTGATATCTGGAATGCCATCAAG
TTTGGCGCTGTGTTGGAAAATGTCGTGTTTGATGAGCATACCAGAGAGGT
GGATTATTCTGACAAATCTGTCACAGAGAACACTCGTGCTGCCTACCCCA
TTGAGTATATCCCCAATGCAAAGATACCATGCGTTGGTCCTCATCCCAAG
AATGTCATCCTTTTGGCATGTGATGCTTTTGGTGTACTCCCACCAGTGAG
CAAGCTGAACCTGGCACAAACAATGTACCATTTCATCAGTGGCTACACTG
CTTTGCTGGAACAGAGGATGGTATTAAGGAGCCAACGGCAACATTTTCAG
CTTGCTTTGGTGCAGCATTTATCATGCTGCATCCTACCAAGTATGCAGCA
ATGCTGGCTGAGAAGATGCAGAAGCATGGTG
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