CF504605

Overview
NameCF504605
Unique NameCF504605
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length431
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CF504605 vs. ExPASy Swiss-Prot
Match: PCKA_BACA2 (Phosphoenolpyruvate carboxykinase [ATP] OS=Bacillus amyloliquefaciens (strain FZB42) GN=pckA PE=3 SV=1)

HSP 1 Score: 129.028 bits (323), Expect = 1.122e-37
Identity = 62/102 (60.78%), Postives = 74/102 (72.55%), Query Frame = 3 
Query:    3 IDLSREKEPDIWNAIKFGAVLENVVFDEHTREVDYSDKSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALL 308
            I+LS EKEP I+ AI+FG+VLENVV DE T E DY +   TENTRAAYPIE I N   P +  HP+ ++ L  DAFGVLPP+SKL   Q MYHF+SGYT+ L
Sbjct:  275 INLSEEKEPQIFRAIRFGSVLENVVLDEKTGEADYDNSFYTENTRAAYPIEMIGNIVQPSIAGHPQAIVFLTADAFGVLPPISKLTKEQAMYHFLSGYTSKL 376

HSP 2 Score: 47.3654 bits (111), Expect = 1.122e-37
Identity = 21/42 (50.00%), Postives = 26/42 (61.90%), Query Frame = 2 
Query:  305 AGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKHG 430
            AGTE G+  P  TFS CFG+ F+ L    YA ML  K+ +HG
Sbjct:  377 AGTERGVTSPQTTFSTCFGSPFLPLPAHVYAEMLGHKIDEHG 418
[Close]
BLAST of CF504605 vs. ExPASy Swiss-Prot
Match: PCKA_CAMJR (Phosphoenolpyruvate carboxykinase [ATP] OS=Campylobacter jejuni (strain RM1221) GN=pckA PE=3 SV=1)

HSP 1 Score: 113.235 bits (282), Expect = 2.480e-37
Identity = 57/100 (57.00%), Postives = 73/100 (73.00%), Query Frame = 3 
Query:    3 IDLSREKEPDIWNAIKFGAVLENVVFDEHTREVDYSDKSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTA 302
            I+L+ E EP+I+ AIK  A+LENVV     + VDY+D S TENTR +YPIE+I N +      HPKN+I L+ DAFG+LPPVSKL+  Q MY+F+SGYTA
Sbjct:  271 INLNPEHEPEIYGAIKRNALLENVVL-RADKSVDYADASKTENTRVSYPIEHIENHEPSLKAGHPKNIIFLSADAFGILPPVSKLSKEQAMYYFLSGYTA 369

HSP 2 Score: 62.003 bits (149), Expect = 2.480e-37
Identity = 28/41 (68.29%), Postives = 32/41 (78.05%), Query Frame = 2 
Query:  305 AGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKH 427
            AGTE GI EP ATFSACFG  F+ LHPT YA +L EK++KH
Sbjct:  372 AGTERGITEPQATFSACFGEPFMPLHPTVYARLLGEKIEKH 412
[Close]
BLAST of CF504605 vs. ExPASy Swiss-Prot
Match: PCKA_CAMJJ (Phosphoenolpyruvate carboxykinase [ATP] OS=Campylobacter jejuni subsp. jejuni serotype O:23/36 (strain 81-176) GN=pckA PE=3 SV=1)

HSP 1 Score: 112.849 bits (281), Expect = 3.231e-37
Identity = 57/100 (57.00%), Postives = 72/100 (72.00%), Query Frame = 3 
Query:    3 IDLSREKEPDIWNAIKFGAVLENVVFDEHTREVDYSDKSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTA 302
            I+L  E EP+I+ AIK  A+LENVV     + VDY+D S TENTR +YPIE+I N +      HPKN+I L+ DAFG+LPPVSKL+  Q MY+F+SGYTA
Sbjct:  271 INLDPEHEPEIYGAIKRNALLENVVL-RADKSVDYADASKTENTRVSYPIEHIENHEPSLKAGHPKNIIFLSADAFGILPPVSKLSKEQAMYYFLSGYTA 369

HSP 2 Score: 62.003 bits (149), Expect = 3.231e-37
Identity = 28/41 (68.29%), Postives = 32/41 (78.05%), Query Frame = 2 
Query:  305 AGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKH 427
            AGTE GI EP ATFSACFG  F+ LHPT YA +L EK++KH
Sbjct:  372 AGTERGITEPQATFSACFGEPFMPLHPTVYARLLGEKIEKH 412
[Close]
BLAST of CF504605 vs. ExPASy Swiss-Prot
Match: PCKA_CAMJE (Phosphoenolpyruvate carboxykinase [ATP] OS=Campylobacter jejuni GN=pckA PE=1 SV=1)

HSP 1 Score: 112.849 bits (281), Expect = 3.231e-37
Identity = 57/100 (57.00%), Postives = 72/100 (72.00%), Query Frame = 3 
Query:    3 IDLSREKEPDIWNAIKFGAVLENVVFDEHTREVDYSDKSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTA 302
            I+L  E EP+I+ AIK  A+LENVV     + VDY+D S TENTR +YPIE+I N +      HPKN+I L+ DAFG+LPPVSKL+  Q MY+F+SGYTA
Sbjct:  271 INLDPEHEPEIYGAIKRNALLENVVL-RADKSVDYADASKTENTRVSYPIEHIENHEPSLKAGHPKNIIFLSADAFGILPPVSKLSKEQAMYYFLSGYTA 369

HSP 2 Score: 62.003 bits (149), Expect = 3.231e-37
Identity = 28/41 (68.29%), Postives = 32/41 (78.05%), Query Frame = 2 
Query:  305 AGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKH 427
            AGTE GI EP ATFSACFG  F+ LHPT YA +L EK++KH
Sbjct:  372 AGTERGITEPQATFSACFGEPFMPLHPTVYARLLGEKIEKH 412
[Close]
BLAST of CF504605 vs. ExPASy Swiss-Prot
Match: PCKA_CAMJ8 (Phosphoenolpyruvate carboxykinase [ATP] OS=Campylobacter jejuni subsp. jejuni serotype O:6 (strain 81116 / NCTC 11828) GN=pckA PE=3 SV=1)

HSP 1 Score: 112.849 bits (281), Expect = 3.231e-37
Identity = 57/100 (57.00%), Postives = 72/100 (72.00%), Query Frame = 3 
Query:    3 IDLSREKEPDIWNAIKFGAVLENVVFDEHTREVDYSDKSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTA 302
            I+L  E EP+I+ AIK  A+LENVV     + VDY+D S TENTR +YPIE+I N +      HPKN+I L+ DAFG+LPPVSKL+  Q MY+F+SGYTA
Sbjct:  271 INLDPEHEPEIYGAIKRNALLENVVL-RADKSVDYADASKTENTRVSYPIEHIENHEPSLKAGHPKNIIFLSADAFGILPPVSKLSKEQAMYYFLSGYTA 369

HSP 2 Score: 62.003 bits (149), Expect = 3.231e-37
Identity = 28/41 (68.29%), Postives = 32/41 (78.05%), Query Frame = 2 
Query:  305 AGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKH 427
            AGTE GI EP ATFSACFG  F+ LHPT YA +L EK++KH
Sbjct:  372 AGTERGITEPQATFSACFGEPFMPLHPTVYARLLGEKIEKH 412
[Close]
BLAST of CF504605 vs. ExPASy Swiss-Prot
Match: PCKA_DINSH (Phosphoenolpyruvate carboxykinase [ATP] OS=Dinoroseobacter shibae (strain DFL 12) GN=pckA PE=3 SV=1)

HSP 1 Score: 122.094 bits (305), Expect = 7.142e-37
Identity = 56/101 (55.45%), Postives = 75/101 (74.26%), Query Frame = 3 
Query:    3 IDLSREKEPDIWNAIK-FGAVLENVVFDEHTREVDYSDKSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTA 302
            I+LS E EP+I+   + FG V+EN+VFD  T ++D+ D S+T N R AYP+ YI NA    +G HPKN+I+L CDAFGVLPP+S+L  AQ MYHF+SG+T+
Sbjct:  279 INLSAEAEPEIYATTEMFGTVIENMVFDPETLDLDFEDDSLTANMRCAYPLNYISNASETALGGHPKNIIMLTCDAFGVLPPISRLTPAQAMYHFLSGFTS 379

HSP 2 Score: 51.6026 bits (122), Expect = 7.142e-37
Identity = 23/42 (54.76%), Postives = 27/42 (64.29%), Query Frame = 2 
Query:  305 AGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKHG 430
            AGTE G+ EP  TFS CFGA F+   P  Y  +L EK+ KHG
Sbjct:  382 AGTERGVTEPEPTFSTCFGAPFMPRRPEVYGKLLQEKIAKHG 423
[Close]
BLAST of CF504605 vs. ExPASy Swiss-Prot
Match: PCKA_STAHJ (Phosphoenolpyruvate carboxykinase [ATP] OS=Staphylococcus haemolyticus (strain JCSC1435) GN=pckA PE=3 SV=1)

HSP 1 Score: 120.553 bits (301), Expect = 7.142e-37
Identity = 57/102 (55.88%), Postives = 74/102 (72.55%), Query Frame = 3 
Query:    3 IDLSREKEPDIWNAIKFGAVLENVVFDEHTREVDYSDKSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALL 308
            I+LS+EKEP I+NAI++G +LEN V +E    VD+ D S TENTRAAYPI +I N  +P    HP  +I L  DAFGVLPP++KL  +Q MYHF+SG+T+ L
Sbjct:  278 INLSKEKEPQIYNAIQYGTILENTVVNERG-VVDFDDNSYTENTRAAYPIHHIDNIVLPSKAAHPNTIIFLTADAFGVLPPIAKLTKSQAMYHFLSGFTSKL 378

HSP 2 Score: 53.1434 bits (126), Expect = 7.142e-37
Identity = 23/41 (56.10%), Postives = 29/41 (70.73%), Query Frame = 2 
Query:  305 AGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKH 427
            AGTE G+ EP  +FS CFGA F+ L+P KYA +L E + KH
Sbjct:  379 AGTERGVTEPEPSFSTCFGAPFLPLNPNKYADLLGELIDKH 419
[Close]
BLAST of CF504605 vs. ExPASy Swiss-Prot
Match: PCKA_BREBN (Phosphoenolpyruvate carboxykinase [ATP] OS=Brevibacillus brevis (strain 47 / JCM 6285 / NBRC 100599) GN=pckA PE=3 SV=1)

HSP 1 Score: 126.331 bits (316), Expect = 7.143e-37
Identity = 60/100 (60.00%), Postives = 70/100 (70.00%), Query Frame = 3 
Query:    3 IDLSREKEPDIWNAIKFGAVLENVVFDEHTREVDYSDKSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTA 302
            + LS E EP IW AI+FG VLENV  +E TR  DY     TENTRAAYP+E IP A IP VG  P  ++ L  D+FGVLPP+SKLN  Q MYHF+SGYT+
Sbjct:  274 VKLSEEGEPQIWKAIQFGTVLENVDVNEATRVADYDSTKYTENTRAAYPVEAIPGAVIPGVGGQPNVIVFLTADSFGVLPPISKLNKEQAMYHFLSGYTS 373

HSP 2 Score: 47.3654 bits (111), Expect = 7.143e-37
Identity = 20/40 (50.00%), Postives = 26/40 (65.00%), Query Frame = 2 
Query:  305 AGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQK 424
            AGTE G+  P   FS CFG+ F+ LHP  YA ML +K+ +
Sbjct:  376 AGTERGVTAPQTEFSTCFGSPFLPLHPVVYAEMLGKKIDE 415
[Close]
BLAST of CF504605 vs. ExPASy Swiss-Prot
Match: PCKA_PARL1 (Phosphoenolpyruvate carboxykinase [ATP] OS=Parvibaculum lavamentivorans (strain DS-1 / DSM 13023 / NCIMB 13966) GN=pckA PE=3 SV=1)

HSP 1 Score: 119.783 bits (299), Expect = 1.578e-36
Identity = 57/101 (56.44%), Postives = 73/101 (72.28%), Query Frame = 3 
Query:    3 IDLSREKEPDIWNAIK-FGAVLENVVFDEHTREVDYSDKSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTA 302
            I LS E EP+I+   + FG VLENVV DE+TRE+D    ++ EN+R AYP+ +IPNA      PHPKN+I+L  DAF VLPPV++L  +Q MYHF+SGYTA
Sbjct:  278 IKLSAEAEPEIYAVTRRFGTVLENVVMDENTRELDLDSAALAENSRGAYPLSFIPNASATGRAPHPKNIIMLTADAFSVLPPVARLTPSQAMYHFLSGYTA 378

HSP 2 Score: 52.7582 bits (125), Expect = 1.578e-36
Identity = 22/41 (53.66%), Postives = 29/41 (70.73%), Query Frame = 2 
Query:  305 AGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKH 427
            AGTE G+ EP ATFS CFGA F+  HPT+Y  +L + + +H
Sbjct:  381 AGTEKGVTEPEATFSTCFGAPFMSRHPTEYGNLLRDLIAQH 421
[Close]
BLAST of CF504605 vs. ExPASy Swiss-Prot
Match: PCKA_AGRT5 (Phosphoenolpyruvate carboxykinase [ATP] OS=Agrobacterium tumefaciens (strain C58 / ATCC 33970) GN=pckA PE=3 SV=2)

HSP 1 Score: 117.472 bits (293), Expect = 1.579e-36
Identity = 58/101 (57.43%), Postives = 75/101 (74.26%), Query Frame = 3 
Query:    3 IDLSREKEPDIWNAI-KFGAVLENVVFDEHTREVDYSDKSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTA 302
            I LS E EP+I+ A  +FG VLENVV DE +R  D++D S+TENTR+AYP+ +IPNA    +  HPK +I+L  DAFGVLPP+++L   Q MYHF+SGYTA
Sbjct:  278 IKLSSEAEPEIYAATNRFGTVLENVVLDE-SRVPDFNDNSLTENTRSAYPLHFIPNASETGIAGHPKTIIMLTADAFGVLPPIARLTPEQAMYHFLSGYTA 377

HSP 2 Score: 55.0694 bits (131), Expect = 1.579e-36
Identity = 24/42 (57.14%), Postives = 29/42 (69.05%), Query Frame = 2 
Query:  305 AGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKHG 430
            AGTE G+ EP ATFS CFGA F+  HP +Y  +L E + KHG
Sbjct:  380 AGTEKGVTEPEATFSTCFGAPFMPRHPAEYGNLLRELIGKHG 421
The following BLAST results are available for this feature:
BLAST of CF504605 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 284
Match NameE-valueIdentityDescription
PCKA_BACA21.122e-3760.78Phosphoenolpyruvate carboxykinase [ATP] OS=Bacillu... [more]
PCKA_CAMJR2.480e-3757.00Phosphoenolpyruvate carboxykinase [ATP] OS=Campylo... [more]
PCKA_CAMJJ3.231e-3757.00Phosphoenolpyruvate carboxykinase [ATP] OS=Campylo... [more]
PCKA_CAMJE3.231e-3757.00Phosphoenolpyruvate carboxykinase [ATP] OS=Campylo... [more]
PCKA_CAMJ83.231e-3757.00Phosphoenolpyruvate carboxykinase [ATP] OS=Campylo... [more]
PCKA_DINSH7.142e-3755.45Phosphoenolpyruvate carboxykinase [ATP] OS=Dinoros... [more]
PCKA_STAHJ7.142e-3755.88Phosphoenolpyruvate carboxykinase [ATP] OS=Staphyl... [more]
PCKA_BREBN7.143e-3760.00Phosphoenolpyruvate carboxykinase [ATP] OS=Breviba... [more]
PCKA_PARL11.578e-3656.44Phosphoenolpyruvate carboxykinase [ATP] OS=Parviba... [more]
PCKA_AGRT51.579e-3657.43Phosphoenolpyruvate carboxykinase [ATP] OS=Agrobac... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_120000-094 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-1_C03 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF504605 ID=CF504605; Name=CF504605; organism=Citrus sinensis; type=EST; length=431bp
GCATTGATCTCTCAAGGGAGAAGGAGCCTGATATCTGGAATGCCATCAAG
TTTGGCGCTGTGTTGGAAAATGTCGTGTTTGATGAGCATACCAGAGAGGT
GGATTATTCTGACAAATCTGTCACAGAGAACACTCGTGCTGCCTACCCCA
TTGAGTATATCCCCAATGCAAAGATACCATGCGTTGGTCCTCATCCCAAG
AATGTCATCCTTTTGGCATGTGATGCTTTTGGTGTACTCCCACCAGTGAG
CAAGCTGAACCTGGCACAAACAATGTACCATTTCATCAGTGGCTACACTG
CTTTGCTGGAACAGAGGATGGTATTAAGGAGCCAACGGCAACATTTTCAG
CTTGCTTTGGTGCAGCATTTATCATGCTGCATCCTACCAAGTATGCAGCA
ATGCTGGCTGAGAAGATGCAGAAGCATGGTG
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