CF504661

Overview
NameCF504661
Unique NameCF504661
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length343
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CF504661 vs. ExPASy Swiss-Prot
Match: TPIS_PONAB (Triosephosphate isomerase OS=Pongo abelii GN=TPI1 PE=2 SV=1)

HSP 1 Score: 56.9954 bits (136), Expect = 8.697e-13
Identity = 27/56 (48.21%), Postives = 34/56 (60.71%), Query Frame = 3 
Query:   93 FLGLVKSSLRPGFHVAAQNCWVKKGGAFTGEISAEMLVNLEIPWVILGHSERRPYF 260
            ++   +  L P   VAAQNC+    GAFTGEIS  M+ +    WV+LGHSERR  F
Sbjct:   48 YIDFARQKLDPKIAVAAQNCYKVTNGAFTGEISPGMIKDYGATWVVLGHSERRHVF 103

HSP 2 Score: 36.5798 bits (83), Expect = 8.697e-13
Identity = 15/29 (51.72%), Postives = 22/29 (75.86%), Query Frame = 1 
Query:  256 ILNELNEFVGDKGAYALSQGLKVIACVGE 342
            +  E +E +G K A+AL++GL VIAC+GE
Sbjct:  102 VFGESDELIGQKVAHALAEGLGVIACIGE 130
[Close]
BLAST of CF504661 vs. ExPASy Swiss-Prot
Match: TPIS_CAEEL (Triosephosphate isomerase OS=Caenorhabditis elegans GN=tpi-1 PE=1 SV=2)

HSP 1 Score: 64.3142 bits (155), Expect = 8.700e-13
Identity = 32/56 (57.14%), Postives = 38/56 (67.86%), Query Frame = 3 
Query:   93 FLGLVKSSLRPGFHVAAQNCWVKKGGAFTGEISAEMLVNLEIPWVILGHSERRPYF 260
            +L   KS L+ G  VAAQNC+    GAFTGEIS  M+ +L + WVILGHSERR  F
Sbjct:   46 YLAYAKSKLKAGVLVAAQNCYKVPKGAFTGEISPAMIKDLGLEWVILGHSERRHVF 101

HSP 2 Score: 29.261 bits (64), Expect = 8.700e-13
Identity = 10/29 (34.48%), Postives = 18/29 (62.07%), Query Frame = 1 
Query:  256 ILNELNEFVGDKGAYALSQGLKVIACVGE 342
            +  E +  + +K  +AL  G+KV+ C+GE
Sbjct:  100 VFGESDALIAEKTVHALEAGIKVVFCIGE 128
[Close]
BLAST of CF504661 vs. ExPASy Swiss-Prot
Match: TPIS_KLUMA (Triosephosphate isomerase OS=Kluyveromyces marxianus GN=TPI1 PE=3 SV=1)

HSP 1 Score: 56.225 bits (134), Expect = 2.474e-12
Identity = 26/42 (61.90%), Postives = 31/42 (73.81%), Query Frame = 3 
Query:  135 VAAQNCWVKKGGAFTGEISAEMLVNLEIPWVILGHSERRPYF 260
            VAAQN ++K  GAFTGE S E + ++   WVILGHSERR YF
Sbjct:   61 VAAQNAYLKASGAFTGENSVEQIKDVGAEWVILGHSERRTYF 102

HSP 2 Score: 35.8094 bits (81), Expect = 2.474e-12
Identity = 14/27 (51.85%), Postives = 20/27 (74.07%), Query Frame = 1 
Query:  262 NELNEFVGDKGAYALSQGLKVIACVGE 342
            +E +EF+ DK  +AL  G+KVI C+GE
Sbjct:  103 HETDEFIADKTKFALDSGVKVILCIGE 129
[Close]
BLAST of CF504661 vs. ExPASy Swiss-Prot
Match: TPIS_DICDI (Triosephosphate isomerase OS=Dictyostelium discoideum GN=tpiA PE=3 SV=1)

HSP 1 Score: 60.077 bits (144), Expect = 7.026e-12
Identity = 29/44 (65.91%), Postives = 34/44 (77.27%), Query Frame = 3 
Query:  129 FHVAAQNCWVKKGGAFTGEISAEMLVNLEIPWVILGHSERRPYF 260
            F V +QNC+    GAFTGEISA MLV+L IP+VI+GHSERR  F
Sbjct:   62 FKVCSQNCYSVAKGAFTGEISANMLVDLGIPYVIIGHSERRNVF 105

HSP 2 Score: 30.4166 bits (67), Expect = 7.026e-12
Identity = 13/29 (44.83%), Postives = 19/29 (65.52%), Query Frame = 1 
Query:  256 ILNELNEFVGDKGAYALSQGLKVIACVGE 342
            + +E +E +  K  YA+S GL VI C+GE
Sbjct:  104 VFSESSELITKKTKYAISLGLTVILCLGE 132
[Close]
BLAST of CF504661 vs. ExPASy Swiss-Prot
Match: TPIS_TRYBB (Triosephosphate isomerase, glycosomal OS=Trypanosoma brucei brucei PE=1 SV=2)

HSP 1 Score: 54.6842 bits (130), Expect = 3.370e-11
Identity = 27/56 (48.21%), Postives = 37/56 (66.07%), Query Frame = 3 
Query:   96 LGLVKSSL-RPGFHVAAQNCWVKKGGAFTGEISAEMLVNLEIPWVILGHSERRPYF 260
            L + K  L  P F +AAQN  + K GAFTGE+S  +L +  + W++LGHSERR Y+
Sbjct:   48 LAMTKERLSHPKFVIAAQNA-IAKSGAFTGEVSLPILKDFGVNWIVLGHSERRAYY 102

HSP 2 Score: 33.4982 bits (75), Expect = 3.370e-11
Identity = 15/26 (57.69%), Postives = 18/26 (69.23%), Query Frame = 1 
Query:  265 ELNEFVGDKGAYALSQGLKVIACVGE 342
            E NE V DK A A++ G  VIAC+GE
Sbjct:  104 ETNEIVADKVAAAVASGFMVIACIGE 129
[Close]
BLAST of CF504661 vs. ExPASy Swiss-Prot
Match: TPIS_ENTHI (Triosephosphate isomerase OS=Entamoeba histolytica GN=TPI PE=1 SV=2)

HSP 1 Score: 53.1434 bits (126), Expect = 5.669e-11
Identity = 24/42 (57.14%), Postives = 32/42 (76.19%), Query Frame = 3 
Query:  135 VAAQNCWVKKGGAFTGEISAEMLVNLEIPWVILGHSERRPYF 260
            V+A+N W K G A+TGE+   MLV+ ++P+VILGHSERR  F
Sbjct:   69 VSAENAWTKSG-AYTGEVHVGMLVDCQVPYVILGHSERRQIF 109

HSP 2 Score: 34.2686 bits (77), Expect = 5.669e-11
Identity = 16/29 (55.17%), Postives = 20/29 (68.97%), Query Frame = 1 
Query:  256 ILNELNEFVGDKGAYALSQGLKVIACVGE 342
            I +E NE V +K   A+  GLKVIAC+GE
Sbjct:  108 IFHESNEQVAEKVKVAIDAGLKVIACIGE 136
[Close]
BLAST of CF504661 vs. ExPASy Swiss-Prot
Match: TPIS_YEAST (Triosephosphate isomerase OS=Saccharomyces cerevisiae GN=TPI1 PE=1 SV=2)

HSP 1 Score: 55.0694 bits (131), Expect = 5.680e-11
Identity = 25/47 (53.19%), Postives = 32/47 (68.09%), Query Frame = 3 
Query:  120 RPGFHVAAQNCWVKKGGAFTGEISAEMLVNLEIPWVILGHSERRPYF 260
            +P   V AQN ++K  GAFTGE S + + ++   WVILGHSERR YF
Sbjct:   56 KPQVTVGAQNAYLKASGAFTGENSVDQIKDVGAKWVILGHSERRSYF 102

HSP 2 Score: 32.3426 bits (72), Expect = 5.680e-11
Identity = 13/27 (48.15%), Postives = 20/27 (74.07%), Query Frame = 1 
Query:  262 NELNEFVGDKGAYALSQGLKVIACVGE 342
            +E ++F+ DK  +AL QG+ VI C+GE
Sbjct:  103 HEDDKFIADKTKFALGQGVGVILCIGE 129
[Close]
BLAST of CF504661 vs. ExPASy Swiss-Prot
Match: TPIS_SCHPO (Triosephosphate isomerase OS=Schizosaccharomyces pombe GN=tpi1 PE=1 SV=4)

HSP 1 Score: 48.1358 bits (113), Expect = 5.680e-11
Identity = 23/57 (40.35%), Postives = 35/57 (61.40%), Query Frame = 3 
Query:   93 FLGLVKSSLRPGFHVAAQNCWVKKGGAFTGEISAEMLVNLEIPWVILGHSERRPYFE 263
            +L   +  ++    V AQN + KK GA+TGE SA+ L++  I + + GHSERR  F+
Sbjct:   47 YLITTRQQVKKDIGVGAQNVFDKKNGAYTGENSAQSLIDAGITYTLTGHSERRTIFK 103

HSP 2 Score: 39.2762 bits (90), Expect = 5.680e-11
Identity = 17/29 (58.62%), Postives = 21/29 (72.41%), Query Frame = 1 
Query:  256 ILNELNEFVGDKGAYALSQGLKVIACVGE 342
            I  E +EFV DK  +AL QGL V+AC+GE
Sbjct:  101 IFKESDEFVADKTKFALEQGLTVVACIGE 129
[Close]
BLAST of CF504661 vs. ExPASy Swiss-Prot
Match: TPIS_KLULA (Triosephosphate isomerase OS=Kluyveromyces lactis GN=TPI1 PE=3 SV=2)

HSP 1 Score: 52.373 bits (124), Expect = 9.570e-11
Identity = 23/42 (54.76%), Postives = 30/42 (71.43%), Query Frame = 3 
Query:  135 VAAQNCWVKKGGAFTGEISAEMLVNLEIPWVILGHSERRPYF 260
            ++AQN + K  GA+TGE S E + ++   WVILGHSERR YF
Sbjct:   61 ISAQNAYSKASGAYTGENSVEQIKDVGAEWVILGHSERRTYF 102

HSP 2 Score: 34.2686 bits (77), Expect = 9.570e-11
Identity = 14/27 (51.85%), Postives = 19/27 (70.37%), Query Frame = 1 
Query:  262 NELNEFVGDKGAYALSQGLKVIACVGE 342
            NE +E V +K  +AL  G+KVI C+GE
Sbjct:  103 NETDEIVAEKTKFALDSGVKVILCIGE 129
[Close]
BLAST of CF504661 vs. ExPASy Swiss-Prot
Match: TPIS_YARLI (Triosephosphate isomerase OS=Yarrowia lipolytica GN=TPI1 PE=3 SV=1)

HSP 1 Score: 52.7582 bits (125), Expect = 9.575e-11
Identity = 29/54 (53.70%), Postives = 35/54 (64.81%), Query Frame = 3 
Query:   93 FLGLVKSSLR-PGFHVAAQNCWVKKGGAFTGEISAEMLVNLEIPWVILGHSERR 251
            +L L K SL+ P   VA QN + K  GAFTGE+S   L ++   WVILGHSERR
Sbjct:   46 YLLLAKESLKKPTVSVAGQNSFDKGDGAFTGEVSVAQLKDVGAKWVILGHSERR 99

HSP 2 Score: 33.8834 bits (76), Expect = 9.575e-11
Identity = 15/29 (51.72%), Postives = 19/29 (65.52%), Query Frame = 1 
Query:  256 ILNELNEFVGDKGAYALSQGLKVIACVGE 342
            I  E +E++ DK  YAL  GL VI C+GE
Sbjct:  101 INKESSEWIADKTKYALDNGLDVILCIGE 129
The following BLAST results are available for this feature:
BLAST of CF504661 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 30
Match NameE-valueIdentityDescription
TPIS_PONAB8.697e-1348.21Triosephosphate isomerase OS=Pongo abelii GN=TPI1 ... [more]
TPIS_CAEEL8.700e-1357.14Triosephosphate isomerase OS=Caenorhabditis elegan... [more]
TPIS_KLUMA2.474e-1261.90Triosephosphate isomerase OS=Kluyveromyces marxian... [more]
TPIS_DICDI7.026e-1265.91Triosephosphate isomerase OS=Dictyostelium discoid... [more]
TPIS_TRYBB3.370e-1148.21Triosephosphate isomerase, glycosomal OS=Trypanoso... [more]
TPIS_ENTHI5.669e-1157.14Triosephosphate isomerase OS=Entamoeba histolytica... [more]
TPIS_YEAST5.680e-1153.19Triosephosphate isomerase OS=Saccharomyces cerevis... [more]
TPIS_SCHPO5.680e-1140.35Triosephosphate isomerase OS=Schizosaccharomyces p... [more]
TPIS_KLULA9.570e-1154.76Triosephosphate isomerase OS=Kluyveromyces lactis ... [more]
TPIS_YARLI9.575e-1153.70Triosephosphate isomerase OS=Yarrowia lipolytica G... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_120000-150 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-1_H05 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF504661 ID=CF504661; Name=CF504661; organism=Citrus sinensis; type=EST; length=343bp
TGAAGAAGTGAAAAAGATAGTGAGCGTACCTCAATGAAGGTCAAGTTCCA
TCTTCTGATGTCGTTGAGGTCGTTGTTAGCCCTCCGTTTGTGTTTCTTGG
ACTGGTGAAGAGTTCGTTGAGGCCTGGCTTTCATGTTGCCGCACAGAATT
GCTGGGTCAAGAAGGGAGGTGCTTTCACCGGTGAGATTAGTGCGGAAATG
CTCGTCAATTTGGAAATTCCTTGGGTCATTCTTGGTCATTCTGAAAGGAG
ACCCTATTTTGAATGAATTAAATGAGTTTGTTGGGGATAAGGGTGCATAT
GCACTTTCTCAAGGTTTGAAGGTGATTGCTTGTGTTGGTGAGA
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