CF504716 | Citrus Genome Database

Overview


Name	CF504716
Unique Name	CF504716
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	247

Libraries


Library Name	Type
Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

BLAST of CF504716 vs. ExPASy Swiss-Prot
Match: HSP71_HUMAN (Heat shock 70 kDa protein 1A/1B OS=Homo sapiens GN=HSPA1A PE=1 SV=5)

HSP 1 Score: 76.6406 bits (187), Expect = 1.452e-29
Identity = 34/45 (75.56%), Postives = 40/45 (88.89%), Query Frame = 3

Query:    3 TFDVSLLTIEEGIFEVKATAGDTHLGGEDFDNRMVNHLCARVQKK 137
            TFDVS+LTI++GIFEVKATAGDTHLGGEDFDNR+VNH     ++K
Sbjct:  204 TFDVSILTIDDGIFEVKATAGDTHLGGEDFDNRLVNHFVEEFKRK 248

HSP 2 Score: 72.4034 bits (176), Expect = 1.452e-29
Identity = 34/44 (77.27%), Postives = 40/44 (90.91%), Query Frame = 1

Query:  115 FVQEFKRKHKKDISGNPRALRRLRTSCERAKRTLSSTAQTTIEI 246
            FV+EFKRKHKKDIS N RA+RRLRT+CERAKRTLSS+ Q ++EI
Sbjct:  241 FVEEFKRKHKKDISQNKRAVRRLRTACERAKRTLSSSTQASLEI 284

BLAST of CF504716 vs. ExPASy Swiss-Prot
Match: HSP71_CANFA (Heat shock 70 kDa protein 1 OS=Canis familiaris GN=HSPA1 PE=2 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 1.452e-29
Identity = 34/45 (75.56%), Postives = 40/45 (88.89%), Query Frame = 3

Query:    3 TFDVSLLTIEEGIFEVKATAGDTHLGGEDFDNRMVNHLCARVQKK 137
            TFDVS+LTI++GIFEVKATAGDTHLGGEDFDNR+VNH     ++K
Sbjct:  204 TFDVSILTIDDGIFEVKATAGDTHLGGEDFDNRLVNHFVEEFKRK 248

HSP 2 Score: 72.4034 bits (176), Expect = 1.452e-29
Identity = 34/44 (77.27%), Postives = 40/44 (90.91%), Query Frame = 1

Query:  115 FVQEFKRKHKKDISGNPRALRRLRTSCERAKRTLSSTAQTTIEI 246
            FV+EFKRKHKKDIS N RA+RRLRT+CERAKRTLSS+ Q ++EI
Sbjct:  241 FVEEFKRKHKKDISQNKRAVRRLRTACERAKRTLSSSTQASLEI 284

BLAST of CF504716 vs. ExPASy Swiss-Prot
Match: HS71B_PIG (Heat shock 70 kDa protein 1B OS=Sus scrofa GN=HSPA1B PE=2 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 1.452e-29
Identity = 34/45 (75.56%), Postives = 40/45 (88.89%), Query Frame = 3

Query:    3 TFDVSLLTIEEGIFEVKATAGDTHLGGEDFDNRMVNHLCARVQKK 137
            TFDVS+LTI++GIFEVKATAGDTHLGGEDFDNR+VNH     ++K
Sbjct:  204 TFDVSILTIDDGIFEVKATAGDTHLGGEDFDNRLVNHFVEEFKRK 248

HSP 2 Score: 72.4034 bits (176), Expect = 1.452e-29
Identity = 34/44 (77.27%), Postives = 40/44 (90.91%), Query Frame = 1

Query:  115 FVQEFKRKHKKDISGNPRALRRLRTSCERAKRTLSSTAQTTIEI 246
            FV+EFKRKHKKDIS N RA+RRLRT+CERAKRTLSS+ Q ++EI
Sbjct:  241 FVEEFKRKHKKDISQNKRAVRRLRTACERAKRTLSSSTQASLEI 284

BLAST of CF504716 vs. ExPASy Swiss-Prot
Match: HS71B_BOVIN (Heat shock 70 kDa protein 1B OS=Bos taurus GN=HSPA1B PE=2 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 1.452e-29
Identity = 34/45 (75.56%), Postives = 40/45 (88.89%), Query Frame = 3

Query:    3 TFDVSLLTIEEGIFEVKATAGDTHLGGEDFDNRMVNHLCARVQKK 137
            TFDVS+LTI++GIFEVKATAGDTHLGGEDFDNR+VNH     ++K
Sbjct:  204 TFDVSILTIDDGIFEVKATAGDTHLGGEDFDNRLVNHFVEEFKRK 248

HSP 2 Score: 72.4034 bits (176), Expect = 1.452e-29
Identity = 34/44 (77.27%), Postives = 40/44 (90.91%), Query Frame = 1

Query:  115 FVQEFKRKHKKDISGNPRALRRLRTSCERAKRTLSSTAQTTIEI 246
            FV+EFKRKHKKDIS N RA+RRLRT+CERAKRTLSS+ Q ++EI
Sbjct:  241 FVEEFKRKHKKDISQNKRAVRRLRTACERAKRTLSSSTQASLEI 284

BLAST of CF504716 vs. ExPASy Swiss-Prot
Match: HS71A_BOVIN (Heat shock 70 kDa protein 1A OS=Bos taurus GN=HSPA1A PE=2 SV=2)

HSP 1 Score: 76.6406 bits (187), Expect = 1.452e-29
Identity = 34/45 (75.56%), Postives = 40/45 (88.89%), Query Frame = 3

Query:    3 TFDVSLLTIEEGIFEVKATAGDTHLGGEDFDNRMVNHLCARVQKK 137
            TFDVS+LTI++GIFEVKATAGDTHLGGEDFDNR+VNH     ++K
Sbjct:  204 TFDVSILTIDDGIFEVKATAGDTHLGGEDFDNRLVNHFVEEFKRK 248

HSP 2 Score: 72.4034 bits (176), Expect = 1.452e-29
Identity = 34/44 (77.27%), Postives = 40/44 (90.91%), Query Frame = 1

Query:  115 FVQEFKRKHKKDISGNPRALRRLRTSCERAKRTLSSTAQTTIEI 246
            FV+EFKRKHKKDIS N RA+RRLRT+CERAKRTLSS+ Q ++EI
Sbjct:  241 FVEEFKRKHKKDISQNKRAVRRLRTACERAKRTLSSSTQASLEI 284

BLAST of CF504716 vs. ExPASy Swiss-Prot
Match: HSP7C_DANRE (Heat shock cognate 71 kDa protein OS=Danio rerio GN=hspa8 PE=2 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 1.890e-29
Identity = 34/45 (75.56%), Postives = 40/45 (88.89%), Query Frame = 3

Query:    3 TFDVSLLTIEEGIFEVKATAGDTHLGGEDFDNRMVNHLCARVQKK 137
            +FDVS+LTIE+GIFEVK+TAGDTHLGGEDFDNRMVNH     ++K
Sbjct:  204 SFDVSILTIEDGIFEVKSTAGDTHLGGEDFDNRMVNHFITEFKRK 248

HSP 2 Score: 72.0182 bits (175), Expect = 1.890e-29
Identity = 34/44 (77.27%), Postives = 39/44 (88.64%), Query Frame = 1

Query:  115 FVQEFKRKHKKDISGNPRALRRLRTSCERAKRTLSSTAQTTIEI 246
            F+ EFKRKHKKDIS N RA+RRLRT+CERAKRTLSS+ Q +IEI
Sbjct:  241 FITEFKRKHKKDISDNKRAVRRLRTACERAKRTLSSSTQASIEI 284

BLAST of CF504716 vs. ExPASy Swiss-Prot
Match: HSP72_PICAN (Heat shock protein 70 2 OS=Pichia angusta GN=HSA2 PE=3 SV=2)

HSP 1 Score: 76.6406 bits (187), Expect = 1.890e-29
Identity = 35/45 (77.78%), Postives = 40/45 (88.89%), Query Frame = 3

Query:    3 TFDVSLLTIEEGIFEVKATAGDTHLGGEDFDNRMVNHLCARVQKK 137
            TFDVSLL+IE+GIFEVKATAGDTHLGGEDFDNR+VNH     ++K
Sbjct:  202 TFDVSLLSIEDGIFEVKATAGDTHLGGEDFDNRLVNHFINEFKRK 246

HSP 2 Score: 72.0182 bits (175), Expect = 1.890e-29
Identity = 34/44 (77.27%), Postives = 40/44 (90.91%), Query Frame = 1

Query:  115 FVQEFKRKHKKDISGNPRALRRLRTSCERAKRTLSSTAQTTIEI 246
            F+ EFKRK+KKDI GN RALRRLRT+CERAK TLSS+AQT++EI
Sbjct:  239 FINEFKRKNKKDICGNQRALRRLRTACERAKTTLSSSAQTSLEI 282

BLAST of CF504716 vs. ExPASy Swiss-Prot
Match: HSP70_ACHKL (Heat shock 70 kDa protein OS=Achlya klebsiana GN=HSP70 PE=2 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 2.460e-29
Identity = 37/45 (82.22%), Postives = 41/45 (91.11%), Query Frame = 3

Query:    3 TFDVSLLTIEEGIFEVKATAGDTHLGGEDFDNRMVNHLCARVQKK 137
            TFDVSLLTIEEGIFEVKATAGDTHLGGEDFDNR+V+H  A  ++K
Sbjct:  206 TFDVSLLTIEEGIFEVKATAGDTHLGGEDFDNRLVDHFTAEFKRK 250

HSP 2 Score: 68.5514 bits (166), Expect = 2.460e-29
Identity = 33/44 (75.00%), Postives = 38/44 (86.36%), Query Frame = 1

Query:  115 FVQEFKRKHKKDISGNPRALRRLRTSCERAKRTLSSTAQTTIEI 246
            F  EFKRKH+KD++ N RALRRLRT+CERAKRTLSS+AQ  IEI
Sbjct:  243 FTAEFKRKHRKDMTQNQRALRRLRTACERAKRTLSSSAQAYIEI 286

BLAST of CF504716 vs. ExPASy Swiss-Prot
Match: HSP7C_ORYLA (Heat shock cognate 71 kDa protein OS=Oryzias latipes GN=hsc70 PE=2 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 3.201e-29
Identity = 36/45 (80.00%), Postives = 41/45 (91.11%), Query Frame = 3

Query:    3 TFDVSLLTIEEGIFEVKATAGDTHLGGEDFDNRMVNHLCARVQKK 137
            TFDVS+LTIE+GIFEVK+TAGDTHLGGEDFDNRMVNH  A  ++K
Sbjct:  204 TFDVSILTIEDGIFEVKSTAGDTHLGGEDFDNRMVNHFIAEFKRK 248

HSP 2 Score: 68.1662 bits (165), Expect = 3.201e-29
Identity = 32/44 (72.73%), Postives = 39/44 (88.64%), Query Frame = 1

Query:  115 FVQEFKRKHKKDISGNPRALRRLRTSCERAKRTLSSTAQTTIEI 246
            F+ EFKRK+KKDIS N RA+RRLR++CERAKRTLSS+ Q +IEI
Sbjct:  241 FIAEFKRKYKKDISDNKRAVRRLRSACERAKRTLSSSTQASIEI 284

BLAST of CF504716 vs. ExPASy Swiss-Prot
Match: HSP71_PICAN (Heat shock protein 70 1 OS=Pichia angusta GN=HSA1 PE=1 SV=2)

HSP 1 Score: 77.0258 bits (188), Expect = 3.203e-29
Identity = 35/45 (77.78%), Postives = 40/45 (88.89%), Query Frame = 3

Query:    3 TFDVSLLTIEEGIFEVKATAGDTHLGGEDFDNRMVNHLCARVQKK 137
            TFDVSLL+I+EGIFEVKATAGDTHLGGEDFDNR+VNH     ++K
Sbjct:  204 TFDVSLLSIDEGIFEVKATAGDTHLGGEDFDNRLVNHFANEFKRK 248

HSP 2 Score: 70.8626 bits (172), Expect = 3.203e-29
Identity = 33/44 (75.00%), Postives = 40/44 (90.91%), Query Frame = 1

Query:  115 FVQEFKRKHKKDISGNPRALRRLRTSCERAKRTLSSTAQTTIEI 246
            F  EFKRK+KKD++ N RALRRLRT+CERAKRTLSS+AQT++EI
Sbjct:  241 FANEFKRKYKKDLTTNQRALRRLRTACERAKRTLSSSAQTSVEI 284

The following BLAST results are available for this feature:

BLAST of CF504716 vs. ExPASy Swiss-Prot

BLAST of CF504716 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500


Match Name	E-value	Identity	Description
HSP71_HUMAN	1.452e-29	75.56	Heat shock 70 kDa protein 1A/1B OS=Homo sapiens GN...	[more]
HSP71_CANFA	1.452e-29	75.56	Heat shock 70 kDa protein 1 OS=Canis familiaris GN...	[more]
HS71B_PIG	1.452e-29	75.56	Heat shock 70 kDa protein 1B OS=Sus scrofa GN=HSPA...	[more]
HS71B_BOVIN	1.452e-29	75.56	Heat shock 70 kDa protein 1B OS=Bos taurus GN=HSPA...	[more]
HS71A_BOVIN	1.452e-29	75.56	Heat shock 70 kDa protein 1A OS=Bos taurus GN=HSPA...	[more]
HSP7C_DANRE	1.890e-29	75.56	Heat shock cognate 71 kDa protein OS=Danio rerio G...	[more]
HSP72_PICAN	1.890e-29	77.78	Heat shock protein 70 2 OS=Pichia angusta GN=HSA2 ...	[more]
HSP70_ACHKL	2.460e-29	82.22	Heat shock 70 kDa protein OS=Achlya klebsiana GN=H...	[more]
HSP7C_ORYLA	3.201e-29	80.00	Heat shock cognate 71 kDa protein OS=Oryzias latip...	[more]
HSP71_PICAN	3.203e-29	77.78	Heat shock protein 70 1 OS=Pichia angusta GN=HSA1 ...	[more]

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Properties


Property Name	Value
Genbank description	USDA-FP_120000-206 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-20_H02 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CF504716 ID=CF504716; Name=CF504716; organism=Citrus sinensis; type=EST; length=247bp
GTACTTTTGATGTGTCTCTTCTTACCATTGAAGAGGGTATCTTTGAAGTT
AAAGCCACAGCTGGTGATACCCATCTTGGTGGTGAGGATTTTGATAACAG
AATGGTTAACCACCTTTGTGCAAGAGTTCAAAAGAAAGCACAAGAAGGAC
ATTAGTGGTAACCCCAGAGCTCTAAGGAGGTTGAGAACTTCCTGTGAAAG
AGCTAAGAGGACTCTCTCATCAACCGCCCAGACCACCATTGAGATTG