CF504716 | Citrus Genome Database

Overview


Name	CF504716
Unique Name	CF504716
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	247

Libraries


Library Name	Type
Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

BLAST of CF504716 vs. ExPASy Swiss-Prot
Match: DNAK_ACTP2 (Chaperone protein dnaK OS=Actinobacillus pleuropneumoniae serotype 5b (strain L20) GN=dnaK PE=2 SV=1)

HSP 1 Score: 52.373 bits (124), Expect = 1.552e-11
Identity = 24/49 (48.98%), Postives = 36/49 (73.47%), Query Frame = 3

Query:    3 TFDVSLLTIE----EGIFEVKATAGDTHLGGEDFDNRMVNHLCARVQKK 137
            TFD+S++ I+    E  FEV AT G+THLGGEDFDNR+++++    +K+
Sbjct:  198 TFDISIIEIDNFDGEQTFEVLATGGNTHLGGEDFDNRVIDYIIDEFKKE 246

HSP 2 Score: 35.8094 bits (81), Expect = 1.552e-11
Identity = 15/43 (34.88%), Postives = 27/43 (62.79%), Query Frame = 1

Query:  118 VQEFKRKHKKDISGNPRALRRLRTSCERAKRTLSSTAQTTIEI 246
            + EFK++   D+  +P AL+R++ + E+AK  LSS   T + +
Sbjct:  240 IDEFKKEQGVDLRNDPMALQRVKEAAEKAKIELSSAQSTEVNL 282

BLAST of CF504716 vs. ExPASy Swiss-Prot
Match: DNAK_MYCTU (Chaperone protein dnaK OS=Mycobacterium tuberculosis GN=dnaK PE=2 SV=2)

HSP 1 Score: 55.4546 bits (132), Expect = 1.553e-11
Identity = 25/38 (65.79%), Postives = 32/38 (84.21%), Query Frame = 3

Query:    3 TFDVSLLTIEEGIFEVKATAGDTHLGGEDFDNRMVNHL 116
            TFDVSLL I EG+ EV+AT+GD HLGG+D+D R+V+ L
Sbjct:  175 TFDVSLLEIGEGVVEVRATSGDNHLGGDDWDQRVVDWL 212

HSP 2 Score: 32.7278 bits (73), Expect = 1.553e-11
Identity = 18/49 (36.73%), Postives = 30/49 (61.22%), Query Frame = 1

Query:  100 EWLTTFVQEFKRKHKKDISGNPRALRRLRTSCERAKRTLSSTAQTTIEI 246
            +WL   V +FK     D++ +  A++RLR + E+AK  LSS+  T+I +
Sbjct:  210 DWL---VDKFKGTSGIDLTKDKMAMQRLREAAEKAKIELSSSQSTSINL 255

BLAST of CF504716 vs. ExPASy Swiss-Prot
Match: DNAK_MYCTA (Chaperone protein dnaK OS=Mycobacterium tuberculosis (strain ATCC 25177 / H37Ra) GN=dnaK PE=2 SV=1)

HSP 1 Score: 55.4546 bits (132), Expect = 1.553e-11
Identity = 25/38 (65.79%), Postives = 32/38 (84.21%), Query Frame = 3

Query:    3 TFDVSLLTIEEGIFEVKATAGDTHLGGEDFDNRMVNHL 116
            TFDVSLL I EG+ EV+AT+GD HLGG+D+D R+V+ L
Sbjct:  175 TFDVSLLEIGEGVVEVRATSGDNHLGGDDWDQRVVDWL 212

HSP 2 Score: 32.7278 bits (73), Expect = 1.553e-11
Identity = 18/49 (36.73%), Postives = 30/49 (61.22%), Query Frame = 1

Query:  100 EWLTTFVQEFKRKHKKDISGNPRALRRLRTSCERAKRTLSSTAQTTIEI 246
            +WL   V +FK     D++ +  A++RLR + E+AK  LSS+  T+I +
Sbjct:  210 DWL---VDKFKGTSGIDLTKDKMAMQRLREAAEKAKIELSSSQSTSINL 255

BLAST of CF504716 vs. ExPASy Swiss-Prot
Match: DNAK_MYCBT (Chaperone protein dnaK OS=Mycobacterium bovis (strain BCG / Tokyo 172 / ATCC 35737 / TMC 1019) GN=dnaK PE=2 SV=1)

HSP 1 Score: 55.4546 bits (132), Expect = 1.553e-11
Identity = 25/38 (65.79%), Postives = 32/38 (84.21%), Query Frame = 3

Query:    3 TFDVSLLTIEEGIFEVKATAGDTHLGGEDFDNRMVNHL 116
            TFDVSLL I EG+ EV+AT+GD HLGG+D+D R+V+ L
Sbjct:  175 TFDVSLLEIGEGVVEVRATSGDNHLGGDDWDQRVVDWL 212

HSP 2 Score: 32.7278 bits (73), Expect = 1.553e-11
Identity = 18/49 (36.73%), Postives = 30/49 (61.22%), Query Frame = 1

Query:  100 EWLTTFVQEFKRKHKKDISGNPRALRRLRTSCERAKRTLSSTAQTTIEI 246
            +WL   V +FK     D++ +  A++RLR + E+AK  LSS+  T+I +
Sbjct:  210 DWL---VDKFKGTSGIDLTKDKMAMQRLREAAEKAKIELSSSQSTSINL 255

BLAST of CF504716 vs. ExPASy Swiss-Prot
Match: DNAK_MYCBP (Chaperone protein dnaK OS=Mycobacterium bovis (strain BCG / Pasteur 1173P2) GN=dnaK PE=2 SV=1)

HSP 1 Score: 55.4546 bits (132), Expect = 1.553e-11
Identity = 25/38 (65.79%), Postives = 32/38 (84.21%), Query Frame = 3

Query:    3 TFDVSLLTIEEGIFEVKATAGDTHLGGEDFDNRMVNHL 116
            TFDVSLL I EG+ EV+AT+GD HLGG+D+D R+V+ L
Sbjct:  175 TFDVSLLEIGEGVVEVRATSGDNHLGGDDWDQRVVDWL 212

HSP 2 Score: 32.7278 bits (73), Expect = 1.553e-11
Identity = 18/49 (36.73%), Postives = 30/49 (61.22%), Query Frame = 1

Query:  100 EWLTTFVQEFKRKHKKDISGNPRALRRLRTSCERAKRTLSSTAQTTIEI 246
            +WL   V +FK     D++ +  A++RLR + E+AK  LSS+  T+I +
Sbjct:  210 DWL---VDKFKGTSGIDLTKDKMAMQRLREAAEKAKIELSSSQSTSINL 255

BLAST of CF504716 vs. ExPASy Swiss-Prot
Match: DNAK_MYCBO (Chaperone protein dnaK OS=Mycobacterium bovis GN=dnaK PE=2 SV=2)

HSP 1 Score: 55.4546 bits (132), Expect = 1.553e-11
Identity = 25/38 (65.79%), Postives = 32/38 (84.21%), Query Frame = 3

Query:    3 TFDVSLLTIEEGIFEVKATAGDTHLGGEDFDNRMVNHL 116
            TFDVSLL I EG+ EV+AT+GD HLGG+D+D R+V+ L
Sbjct:  175 TFDVSLLEIGEGVVEVRATSGDNHLGGDDWDQRVVDWL 212

HSP 2 Score: 32.7278 bits (73), Expect = 1.553e-11
Identity = 18/49 (36.73%), Postives = 30/49 (61.22%), Query Frame = 1

Query:  100 EWLTTFVQEFKRKHKKDISGNPRALRRLRTSCERAKRTLSSTAQTTIEI 246
            +WL   V +FK     D++ +  A++RLR + E+AK  LSS+  T+I +
Sbjct:  210 DWL---VDKFKGTSGIDLTKDKMAMQRLREAAEKAKIELSSSQSTSINL 255

BLAST of CF504716 vs. ExPASy Swiss-Prot
Match: DNAK_MACCJ (Chaperone protein dnaK OS=Macrococcus caseolyticus (strain JCSC5402) GN=dnaK PE=2 SV=1)

HSP 1 Score: 50.8322 bits (120), Expect = 1.554e-11
Identity = 21/45 (46.67%), Postives = 34/45 (75.56%), Query Frame = 3

Query:    3 TFDVSLLTIEEGIFEVKATAGDTHLGGEDFDNRMVNHLCARVQKK 137
            TFDVS+L + +G+FEV +T+GD  LGG+DFD  ++++L    +K+
Sbjct:  173 TFDVSILELGDGVFEVLSTSGDNKLGGDDFDQVIIDYLVEEFKKE 217

HSP 2 Score: 37.3502 bits (85), Expect = 1.554e-11
Identity = 16/43 (37.21%), Postives = 30/43 (69.77%), Query Frame = 1

Query:  118 VQEFKRKHKKDISGNPRALRRLRTSCERAKRTLSSTAQTTIEI 246
            V+EFK+++  D+S +  A++RL+ + E+AK+ LS  + T I +
Sbjct:  211 VEEFKKENGLDLSQDKMAMQRLKDAAEKAKKDLSGVSSTQISL 253

BLAST of CF504716 vs. ExPASy Swiss-Prot
Match: DNAK_ANATD (Chaperone protein dnaK OS=Anaerocellum thermophilum (strain DSM 6725 / Z-1320) GN=dnaK PE=2 SV=1)

HSP 1 Score: 50.8322 bits (120), Expect = 1.554e-11
Identity = 21/45 (46.67%), Postives = 33/45 (73.33%), Query Frame = 3

Query:    3 TFDVSLLTIEEGIFEVKATAGDTHLGGEDFDNRMVNHLCARVQKK 137
            TFDVS+L I +G+ EV AT+G+  LGG+DFD R+++++     K+
Sbjct:  174 TFDVSILEIGDGVIEVLATSGNNRLGGDDFDQRIIDYIADEFMKE 218

HSP 2 Score: 37.3502 bits (85), Expect = 1.554e-11
Identity = 19/41 (46.34%), Postives = 27/41 (65.85%), Query Frame = 1

Query:  124 EFKRKHKKDISGNPRALRRLRTSCERAKRTLSSTAQTTIEI 246
            EF ++H  D+  +  AL+RL+ + ERAK  LSS  QTTI +
Sbjct:  214 EFMKEHGIDLRQDKVALQRLKDAAERAKIELSSALQTTINL 254

BLAST of CF504716 vs. ExPASy Swiss-Prot
Match: HSP70_LEIDO (Heat shock 70 kDa protein OS=Leishmania donovani GN=HSP70 PE=3 SV=1)

HSP 1 Score: 67.781 bits (164), Expect = 1.962e-11
Identity = 32/45 (71.11%), Postives = 36/45 (80.00%), Query Frame = 3

Query:    3 TFDVSLLTIEEGIFEVKATAGDTHLGGEDFDNRMVNHLCARVQKK 137
            TFDVSLLTI+ GIFEVKAT GDTHLGGEDFDNR+V       ++K
Sbjct:  205 TFDVSLLTIDGGIFEVKATNGDTHLGGEDFDNRLVTFFTEEFKRK 249

BLAST of CF504716 vs. ExPASy Swiss-Prot
Match: DNAK_LEPIN (Chaperone protein dnaK OS=Leptospira interrogans GN=dnaK PE=2 SV=1)

HSP 1 Score: 53.1434 bits (126), Expect = 2.014e-11
Identity = 21/31 (67.74%), Postives = 28/31 (90.32%), Query Frame = 3

Query:    3 TFDVSLLTIEEGIFEVKATAGDTHLGGEDFD 95
            TFD+S+L + +G+FEVK+T GDTHLGG+DFD
Sbjct:  199 TFDISILELADGVFEVKSTNGDTHLGGDDFD 229

HSP 2 Score: 34.6538 bits (78), Expect = 2.014e-11
Identity = 18/49 (36.73%), Postives = 28/49 (57.14%), Query Frame = 1

Query:  100 EWLTTFVQEFKRKHKKDISGNPRALRRLRTSCERAKRTLSSTAQTTIEI 246
            EW+   + EFK +   DIS +   ++RL+ + E+AK  LS T  T I +
Sbjct:  234 EWM---ISEFKNQTGIDISADKNTVQRLKEAAEKAKIELSGTMSTQINL 279

The following BLAST results are available for this feature:

BLAST of CF504716 vs. ExPASy Swiss-Prot

BLAST of CF504716 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500


Match Name	E-value	Identity	Description
DNAK_ACTP2	1.552e-11	48.98	Chaperone protein dnaK OS=Actinobacillus pleuropne...	[more]
DNAK_MYCTU	1.553e-11	65.79	Chaperone protein dnaK OS=Mycobacterium tuberculos...	[more]
DNAK_MYCTA	1.553e-11	65.79	Chaperone protein dnaK OS=Mycobacterium tuberculos...	[more]
DNAK_MYCBT	1.553e-11	65.79	Chaperone protein dnaK OS=Mycobacterium bovis (str...	[more]
DNAK_MYCBP	1.553e-11	65.79	Chaperone protein dnaK OS=Mycobacterium bovis (str...	[more]
DNAK_MYCBO	1.553e-11	65.79	Chaperone protein dnaK OS=Mycobacterium bovis GN=d...	[more]
DNAK_MACCJ	1.554e-11	46.67	Chaperone protein dnaK OS=Macrococcus caseolyticus...	[more]
DNAK_ANATD	1.554e-11	46.67	Chaperone protein dnaK OS=Anaerocellum thermophilu...	[more]
HSP70_LEIDO	1.962e-11	71.11	Heat shock 70 kDa protein OS=Leishmania donovani G...	[more]
DNAK_LEPIN	2.014e-11	67.74	Chaperone protein dnaK OS=Leptospira interrogans G...	[more]

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Properties


Property Name	Value
Genbank description	USDA-FP_120000-206 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-20_H02 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CF504716 ID=CF504716; Name=CF504716; organism=Citrus sinensis; type=EST; length=247bp
GTACTTTTGATGTGTCTCTTCTTACCATTGAAGAGGGTATCTTTGAAGTT
AAAGCCACAGCTGGTGATACCCATCTTGGTGGTGAGGATTTTGATAACAG
AATGGTTAACCACCTTTGTGCAAGAGTTCAAAAGAAAGCACAAGAAGGAC
ATTAGTGGTAACCCCAGAGCTCTAAGGAGGTTGAGAACTTCCTGTGAAAG
AGCTAAGAGGACTCTCTCATCAACCGCCCAGACCACCATTGAGATTG