CF504716 | Citrus Genome Database

Overview


Name	CF504716
Unique Name	CF504716
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	247

Libraries


Library Name	Type
Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of CF504716 vs. ExPASy Swiss-Prot
Match: HSP72_HUMAN (Heat shock-related 70 kDa protein 2 OS=Homo sapiens GN=HSPA2 PE=1 SV=1)

HSP 1 Score: 77.7962 bits (190), Expect = 1.560e-28
Identity = 35/45 (77.78%), Postives = 41/45 (91.11%), Query Frame = 3

Query:    3 TFDVSLLTIEEGIFEVKATAGDTHLGGEDFDNRMVNHLCARVQKK 137
            TFDVS+LTIE+GIFEVK+TAGDTHLGGEDFDNRMV+HL    ++K
Sbjct:  207 TFDVSILTIEDGIFEVKSTAGDTHLGGEDFDNRMVSHLAEEFKRK 251

HSP 2 Score: 67.781 bits (164), Expect = 1.560e-28
Identity = 32/42 (76.19%), Postives = 37/42 (88.10%), Query Frame = 1

Query:  121 QEFKRKHKKDISGNPRALRRLRTSCERAKRTLSSTAQTTIEI 246
            +EFKRKHKKDI  N RA+RRLRT+CERAKRTLSS+ Q +IEI
Sbjct:  246 EEFKRKHKKDIGPNKRAVRRLRTACERAKRTLSSSTQASIEI 287

[Close]

BLAST of CF504716 vs. ExPASy Swiss-Prot
Match: HSP72_RAT (Heat shock-related 70 kDa protein 2 OS=Rattus norvegicus GN=Hspa2 PE=2 SV=2)

HSP 1 Score: 77.7962 bits (190), Expect = 1.560e-28
Identity = 35/45 (77.78%), Postives = 41/45 (91.11%), Query Frame = 3

Query:    3 TFDVSLLTIEEGIFEVKATAGDTHLGGEDFDNRMVNHLCARVQKK 137
            TFDVS+LTIE+GIFEVK+TAGDTHLGGEDFDNRMV+HL    ++K
Sbjct:  207 TFDVSILTIEDGIFEVKSTAGDTHLGGEDFDNRMVSHLAEEFKRK 251

HSP 2 Score: 67.781 bits (164), Expect = 1.560e-28
Identity = 32/42 (76.19%), Postives = 37/42 (88.10%), Query Frame = 1

Query:  121 QEFKRKHKKDISGNPRALRRLRTSCERAKRTLSSTAQTTIEI 246
            +EFKRKHKKDI  N RA+RRLRT+CERAKRTLSS+ Q +IEI
Sbjct:  246 EEFKRKHKKDIGPNKRAVRRLRTACERAKRTLSSSTQASIEI 287

[Close]

BLAST of CF504716 vs. ExPASy Swiss-Prot
Match: HSP72_MOUSE (Heat shock-related 70 kDa protein 2 OS=Mus musculus GN=Hspa2 PE=1 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 3.443e-28
Identity = 34/45 (75.56%), Postives = 41/45 (91.11%), Query Frame = 3

Query:    3 TFDVSLLTIEEGIFEVKATAGDTHLGGEDFDNRMVNHLCARVQKK 137
            TFDVS+LTIE+GIFE+K+TAGDTHLGGEDFDNRMV+HL    ++K
Sbjct:  207 TFDVSILTIEDGIFELKSTAGDTHLGGEDFDNRMVSHLAEEFKRK 251

HSP 2 Score: 67.781 bits (164), Expect = 3.443e-28
Identity = 32/42 (76.19%), Postives = 37/42 (88.10%), Query Frame = 1

Query:  121 QEFKRKHKKDISGNPRALRRLRTSCERAKRTLSSTAQTTIEI 246
            +EFKRKHKKDI  N RA+RRLRT+CERAKRTLSS+ Q +IEI
Sbjct:  246 EEFKRKHKKDIGPNKRAVRRLRTACERAKRTLSSSTQASIEI 287

[Close]

BLAST of CF504716 vs. ExPASy Swiss-Prot
Match: HSP70_BRELC (Heat shock 70 kDa protein OS=Bremia lactucae GN=HSP70 PE=3 SV=1)

HSP 1 Score: 72.0182 bits (175), Expect = 4.478e-28
Identity = 33/45 (73.33%), Postives = 39/45 (86.67%), Query Frame = 3

Query:    3 TFDVSLLTIEEGIFEVKATAGDTHLGGEDFDNRMVNHLCARVQKK 137
            TFDVSLL+IEEGIFEVK+TA DTHLGGEDFDNR+V+H     ++K
Sbjct:  206 TFDVSLLSIEEGIFEVKSTARDTHLGGEDFDNRLVDHFTQEFKRK 250

HSP 2 Score: 72.0182 bits (175), Expect = 4.478e-28
Identity = 35/44 (79.55%), Postives = 39/44 (88.64%), Query Frame = 1

Query:  115 FVQEFKRKHKKDISGNPRALRRLRTSCERAKRTLSSTAQTTIEI 246
            F QEFKRKH+KDI+ N RALRRLRT+CERAKRTLSS+AQ  IEI
Sbjct:  243 FTQEFKRKHRKDITENQRALRRLRTACERAKRTLSSSAQAYIEI 286

[Close]

BLAST of CF504716 vs. ExPASy Swiss-Prot
Match: HSP7C_CRIGR (Heat shock cognate 71 kDa protein OS=Cricetulus griseus GN=HSPA8 PE=2 SV=1)

HSP 1 Score: 77.7962 bits (190), Expect = 4.481e-28
Identity = 35/44 (79.55%), Postives = 40/44 (90.91%), Query Frame = 3

Query:    3 TFDVSLLTIEEGIFEVKATAGDTHLGGEDFDNRMVNHLCARVQK 134
            TFDVS+LTIE+GIFEVK+TAGDTHLGGEDFDNRMVNH  A  ++
Sbjct:  204 TFDVSILTIEDGIFEVKSTAGDTHLGGEDFDNRMVNHFIAEFKR 247

HSP 2 Score: 66.2402 bits (160), Expect = 4.481e-28
Identity = 32/44 (72.73%), Postives = 37/44 (84.09%), Query Frame = 1

Query:  115 FVQEFKRKHKKDISGNPRALRRLRTSCERAKRTLSSTAQTTIEI 246
            F+ EFKR  KKDIS N RA+RRLRT+CERAKRTLSS+ Q +IEI
Sbjct:  241 FIAEFKRNDKKDISENKRAVRRLRTACERAKRTLSSSTQASIEI 284

[Close]

BLAST of CF504716 vs. ExPASy Swiss-Prot
Match: HSP72_BOVIN (Heat shock-related 70 kDa protein 2 OS=Bos taurus GN=HSPA2 PE=2 SV=2)

HSP 1 Score: 75.485 bits (184), Expect = 5.835e-28
Identity = 34/45 (75.56%), Postives = 40/45 (88.89%), Query Frame = 3

Query:    3 TFDVSLLTIEEGIFEVKATAGDTHLGGEDFDNRMVNHLCARVQKK 137
            TFDVS+LTIE+GIFEVK+TAGDTHLGGEDFDN MV+HL    ++K
Sbjct:  207 TFDVSILTIEDGIFEVKSTAGDTHLGGEDFDNSMVSHLAEEFKRK 251

HSP 2 Score: 68.1662 bits (165), Expect = 5.835e-28
Identity = 32/42 (76.19%), Postives = 38/42 (90.48%), Query Frame = 1

Query:  121 QEFKRKHKKDISGNPRALRRLRTSCERAKRTLSSTAQTTIEI 246
            +EFKRKHKKDI+ N RA+RRLRT+CERAKRTLSS+ Q +IEI
Sbjct:  246 EEFKRKHKKDIAPNKRAVRRLRTACERAKRTLSSSTQASIEI 287

[Close]

BLAST of CF504716 vs. ExPASy Swiss-Prot
Match: HSP70_PLEWA (Heat shock 70 kDa protein OS=Pleurodeles waltlii GN=HSP70 PE=2 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 7.594e-28
Identity = 35/45 (77.78%), Postives = 40/45 (88.89%), Query Frame = 3

Query:    3 TFDVSLLTIEEGIFEVKATAGDTHLGGEDFDNRMVNHLCARVQKK 137
            TFDVS+LTI++GIFEVKATAGDTHLGGEDFDNRMVNH     ++K
Sbjct:  206 TFDVSILTIDDGIFEVKATAGDTHLGGEDFDNRMVNHFHEPFKRK 250

HSP 2 Score: 67.0106 bits (162), Expect = 7.594e-28
Identity = 32/44 (72.73%), Postives = 38/44 (86.36%), Query Frame = 1

Query:  115 FVQEFKRKHKKDISGNPRALRRLRTSCERAKRTLSSTAQTTIEI 246
            F + FKRKHKKDI+ N RA+RRLRT+CERAKRTLSS+ Q +IEI
Sbjct:  243 FHEPFKRKHKKDITKNKRAVRRLRTACERAKRTLSSSTQASIEI 286

[Close]

BLAST of CF504716 vs. ExPASy Swiss-Prot
Match: HSP74_ANOAL (Heat shock protein 70 B2 OS=Anopheles albimanus GN=HSP70B2 PE=3 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 9.887e-28
Identity = 35/44 (79.55%), Postives = 40/44 (90.91%), Query Frame = 1

Query:  115 FVQEFKRKHKKDISGNPRALRRLRTSCERAKRTLSSTAQTTIEI 246
            FV+EFKRKHKKD+S N RALRRLRT+CERAKRTLSS+ + TIEI
Sbjct:  240 FVEEFKRKHKKDLSKNARALRRLRTACERAKRTLSSSTEATIEI 283

HSP 2 Score: 68.9366 bits (167), Expect = 9.887e-28
Identity = 32/46 (69.57%), Postives = 39/46 (84.78%), Query Frame = 3

Query:    3 TFDVSLLTIEEG-IFEVKATAGDTHLGGEDFDNRMVNHLCARVQKK 137
            TFDVS+LTI+EG +FEV++TAGDTHLGGEDFDNRMV H     ++K
Sbjct:  202 TFDVSILTIDEGSLFEVRSTAGDTHLGGEDFDNRMVGHFVEEFKRK 247

[Close]

BLAST of CF504716 vs. ExPASy Swiss-Prot
Match: HSP77_HUMAN (Putative heat shock 70 kDa protein 7 OS=Homo sapiens GN=HSPA7 PE=5 SV=2)

HSP 1 Score: 73.1738 bits (178), Expect = 9.963e-28
Identity = 32/45 (71.11%), Postives = 39/45 (86.67%), Query Frame = 3

Query:    3 TFDVSLLTIEEGIFEVKATAGDTHLGGEDFDNRMVNHLCARVQKK 137
            TFDVS+L+I+ G+FEVKATAGDTHLGGEDFDNR+VNH     ++K
Sbjct:  206 TFDVSVLSIDAGVFEVKATAGDTHLGGEDFDNRLVNHFMEEFRRK 250

HSP 2 Score: 69.707 bits (169), Expect = 9.963e-28
Identity = 32/44 (72.73%), Postives = 39/44 (88.64%), Query Frame = 1

Query:  115 FVQEFKRKHKKDISGNPRALRRLRTSCERAKRTLSSTAQTTIEI 246
            F++EF+RKH KD+SGN RALRRLRT+CERAKRT SS+ Q T+EI
Sbjct:  243 FMEEFRRKHGKDLSGNKRALRRLRTACERAKRTPSSSTQATLEI 286

[Close]

BLAST of CF504716 vs. ExPASy Swiss-Prot
Match: HSP73_YEAST (Heat shock protein SSA3 OS=Saccharomyces cerevisiae GN=SSA3 PE=1 SV=3)

HSP 1 Score: 79.7221 bits (195), Expect = 1.675e-27
Identity = 35/48 (72.92%), Postives = 43/48 (89.58%), Query Frame = 3

Query:    3 TFDVSLLTIEEGIFEVKATAGDTHLGGEDFDNRMVNHLCARVQKKAQE 146
            TFDVSLL+I+EG+FEVKATAGDTHLGGEDFDNR+VNHL    ++K ++
Sbjct:  202 TFDVSLLSIDEGVFEVKATAGDTHLGGEDFDNRLVNHLATEFKRKTKK 249

HSP 2 Score: 62.3882 bits (150), Expect = 1.675e-27
Identity = 31/41 (75.61%), Postives = 36/41 (87.80%), Query Frame = 1

Query:  124 EFKRKHKKDISGNPRALRRLRTSCERAKRTLSSTAQTTIEI 246
            EFKRK KKDIS N R+LRRLRT+ ERAKR LSS++QT+IEI
Sbjct:  242 EFKRKTKKDISNNQRSLRRLRTAAERAKRALSSSSQTSIEI 282

The following BLAST results are available for this feature:

BLAST of CF504716 vs. ExPASy Swiss-Prot

BLAST of CF504716 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500


Match Name	E-value	Identity	Description
HSP72_HUMAN	1.560e-28	77.78	Heat shock-related 70 kDa protein 2 OS=Homo sapien...	[more]
HSP72_RAT	1.560e-28	77.78	Heat shock-related 70 kDa protein 2 OS=Rattus norv...	[more]
HSP72_MOUSE	3.443e-28	75.56	Heat shock-related 70 kDa protein 2 OS=Mus musculu...	[more]
HSP70_BRELC	4.478e-28	73.33	Heat shock 70 kDa protein OS=Bremia lactucae GN=HS...	[more]
HSP7C_CRIGR	4.481e-28	79.55	Heat shock cognate 71 kDa protein OS=Cricetulus gr...	[more]
HSP72_BOVIN	5.835e-28	75.56	Heat shock-related 70 kDa protein 2 OS=Bos taurus ...	[more]
HSP70_PLEWA	7.594e-28	77.78	Heat shock 70 kDa protein OS=Pleurodeles waltlii G...	[more]
HSP74_ANOAL	9.887e-28	79.55	Heat shock protein 70 B2 OS=Anopheles albimanus GN...	[more]
HSP77_HUMAN	9.963e-28	71.11	Putative heat shock 70 kDa protein 7 OS=Homo sapie...	[more]
HSP73_YEAST	1.675e-27	72.92	Heat shock protein SSA3 OS=Saccharomyces cerevisia...	[more]

Pages

Properties


Property Name	Value
Genbank description	USDA-FP_120000-206 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-20_H02 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CF504716 ID=CF504716; Name=CF504716; organism=Citrus sinensis; type=EST; length=247bp
GTACTTTTGATGTGTCTCTTCTTACCATTGAAGAGGGTATCTTTGAAGTT
AAAGCCACAGCTGGTGATACCCATCTTGGTGGTGAGGATTTTGATAACAG
AATGGTTAACCACCTTTGTGCAAGAGTTCAAAAGAAAGCACAAGAAGGAC
ATTAGTGGTAACCCCAGAGCTCTAAGGAGGTTGAGAACTTCCTGTGAAAG
AGCTAAGAGGACTCTCTCATCAACCGCCCAGACCACCATTGAGATTG