CF509642 | Citrus Genome Database

Overview


Name	CF509642
Unique Name	CF509642
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	433

Libraries


Library Name	Type
Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of CF509642 vs. ExPASy Swiss-Prot
Match: MAO1_AERS4 (NAD-dependent malic enzyme OS=Aeromonas salmonicida (strain A449) GN=sfcA PE=3 SV=1)

HSP 1 Score: 59.6918 bits (143), Expect = 1.566e-16
Identity = 26/51 (50.98%), Postives = 38/51 (74.51%), Query Frame = 1

Query:    1 ITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVL 153
            + +DVGTNN+QLL D FY+G R  R +G+EYAE +D F+ AVK+ + + +L
Sbjct:  193 VVLDVGTNNQQLLSDPFYMGWRNPRISGEEYAEFVDAFIQAVKRRWPDILL 243

HSP 2 Score: 34.2686 bits (77), Expect = 1.566e-16
Identity = 16/28 (57.14%), Postives = 18/28 (64.29%), Query Frame = 3

Query:  165 FEDFANHNAFELLAKYGTTHLVFNDDIQ 248
            FEDFA +NA  LL +Y      FNDDIQ
Sbjct:  245 FEDFAQNNAMPLLNRYKNELCCFNDDIQ 272

HSP 3 Score: 31.9574 bits (71), Expect = 1.566e-16
Identity = 15/29 (51.72%), Postives = 19/29 (65.52%), Query Frame = 2

Query:  302 LAEHRFLFLGAGEAGTGIAELIALEISKQ 388
            L+E R  FLGAG AG GIAE I  ++  +
Sbjct:  293 LSEKRVAFLGAGSAGCGIAEQIVAQMKAE 321

[Close]

BLAST of CF509642 vs. ExPASy Swiss-Prot
Match: MAO1_AERHH (NAD-dependent malic enzyme OS=Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240) GN=sfcA PE=3 SV=2)

HSP 1 Score: 59.6918 bits (143), Expect = 1.566e-16
Identity = 26/51 (50.98%), Postives = 38/51 (74.51%), Query Frame = 1

Query:    1 ITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVL 153
            + +DVGTNN+QLL D FY+G R  R +G+EYAE +D F+ AVK+ + + +L
Sbjct:  193 VVLDVGTNNQQLLNDPFYMGWRNPRISGEEYAEFVDAFIQAVKRRWPDILL 243

HSP 2 Score: 34.2686 bits (77), Expect = 1.566e-16
Identity = 16/28 (57.14%), Postives = 18/28 (64.29%), Query Frame = 3

Query:  165 FEDFANHNAFELLAKYGTTHLVFNDDIQ 248
            FEDFA +NA  LL +Y      FNDDIQ
Sbjct:  245 FEDFAQNNAMPLLNRYKNELCCFNDDIQ 272

HSP 3 Score: 31.9574 bits (71), Expect = 1.566e-16
Identity = 15/29 (51.72%), Postives = 19/29 (65.52%), Query Frame = 2

Query:  302 LAEHRFLFLGAGEAGTGIAELIALEISKQ 388
            L+E R  FLGAG AG GIAE I  ++  +
Sbjct:  293 LSEKRVAFLGAGSAGCGIAEQIVAQMKAE 321

[Close]

BLAST of CF509642 vs. ExPASy Swiss-Prot
Match: MAO1_SHISS (NAD-dependent malic enzyme OS=Shigella sonnei (strain Ss046) GN=sfcA PE=3 SV=2)

HSP 1 Score: 57.3806 bits (137), Expect = 4.397e-16
Identity = 26/51 (50.98%), Postives = 35/51 (68.63%), Query Frame = 1

Query:    1 ITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVL 153
            + +DVGTNN+QLL D  Y+G R  R T  EY E +DEF+ AVKQ + + +L
Sbjct:  193 VVLDVGTNNQQLLNDPLYMGWRNPRITDDEYYEFVDEFIQAVKQRWPDVLL 243

HSP 2 Score: 33.8834 bits (76), Expect = 4.397e-16
Identity = 18/43 (41.86%), Postives = 25/43 (58.14%), Query Frame = 2

Query:  302 LAEHRFLFLGAGEAGTGIAELIALEISKQTKAPVEETPEEDMS 430
            L+E + +FLGAG AG GIAE+I           + +TP E +S
Sbjct:  293 LSEKKIVFLGAGSAGCGIAEMI-----------IAQTPREGLS 324

HSP 3 Score: 33.113 bits (74), Expect = 4.397e-16
Identity = 16/28 (57.14%), Postives = 17/28 (60.71%), Query Frame = 3

Query:  165 FEDFANHNAFELLAKYGTTHLVFNDDIQ 248
            FEDFA  NA  LL +Y      FNDDIQ
Sbjct:  245 FEDFAQKNAMPLLNRYRNEICSFNDDIQ 272

[Close]

BLAST of CF509642 vs. ExPASy Swiss-Prot
Match: MAO1_SHIFL (NAD-dependent malic enzyme OS=Shigella flexneri GN=sfcA PE=3 SV=4)

HSP 1 Score: 57.3806 bits (137), Expect = 5.691e-16
Identity = 26/51 (50.98%), Postives = 35/51 (68.63%), Query Frame = 1

Query:    1 ITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVL 153
            + +DVGTNN+QLL D  Y+G R  R T  EY E +DEF+ AVKQ + + +L
Sbjct:  193 VVLDVGTNNQQLLNDPLYMGWRNPRITDDEYYEFVDEFIQAVKQRWPDVLL 243

HSP 2 Score: 33.4982 bits (75), Expect = 5.691e-16
Identity = 14/29 (48.28%), Postives = 21/29 (72.41%), Query Frame = 2

Query:  302 LAEHRFLFLGAGEAGTGIAELIALEISKQ 388
            L+E + +FLGAG AG GIAE+I  +  ++
Sbjct:  293 LSEKKIVFLGAGSAGCGIAEMIIAQTQRE 321

HSP 3 Score: 33.113 bits (74), Expect = 5.691e-16
Identity = 16/28 (57.14%), Postives = 17/28 (60.71%), Query Frame = 3

Query:  165 FEDFANHNAFELLAKYGTTHLVFNDDIQ 248
            FEDFA  NA  LL +Y      FNDDIQ
Sbjct:  245 FEDFAQKNAMPLLNRYRNEICSFNDDIQ 272

[Close]

BLAST of CF509642 vs. ExPASy Swiss-Prot
Match: MAO1_SHIF8 (NAD-dependent malic enzyme OS=Shigella flexneri serotype 5b (strain 8401) GN=sfcA PE=3 SV=1)

HSP 1 Score: 57.3806 bits (137), Expect = 5.691e-16
Identity = 26/51 (50.98%), Postives = 35/51 (68.63%), Query Frame = 1

Query:    1 ITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVL 153
            + +DVGTNN+QLL D  Y+G R  R T  EY E +DEF+ AVKQ + + +L
Sbjct:  193 VVLDVGTNNQQLLNDPLYMGWRNPRITDDEYYEFVDEFIQAVKQRWPDVLL 243

HSP 2 Score: 33.4982 bits (75), Expect = 5.691e-16
Identity = 14/29 (48.28%), Postives = 21/29 (72.41%), Query Frame = 2

Query:  302 LAEHRFLFLGAGEAGTGIAELIALEISKQ 388
            L+E + +FLGAG AG GIAE+I  +  ++
Sbjct:  293 LSEKKIVFLGAGSAGCGIAEMIIAQTQRE 321

HSP 3 Score: 33.113 bits (74), Expect = 5.691e-16
Identity = 16/28 (57.14%), Postives = 17/28 (60.71%), Query Frame = 3

Query:  165 FEDFANHNAFELLAKYGTTHLVFNDDIQ 248
            FEDFA  NA  LL +Y      FNDDIQ
Sbjct:  245 FEDFAQKNAMPLLNRYRNEICSFNDDIQ 272

[Close]

BLAST of CF509642 vs. ExPASy Swiss-Prot
Match: MAO1_ESCF3 (NAD-dependent malic enzyme OS=Escherichia fergusonii (strain ATCC 35469 / DSM 13698 / CDC 0568-73) GN=sfcA PE=3 SV=1)

HSP 1 Score: 57.3806 bits (137), Expect = 5.691e-16
Identity = 26/51 (50.98%), Postives = 35/51 (68.63%), Query Frame = 1

Query:    1 ITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVL 153
            + +DVGTNN+QLL D  Y+G R  R T  EY E +DEF+ AVKQ + + +L
Sbjct:  193 VVLDVGTNNQQLLNDPLYMGWRNPRITDDEYYEFVDEFIQAVKQRWPDVLL 243

HSP 2 Score: 33.4982 bits (75), Expect = 5.691e-16
Identity = 14/29 (48.28%), Postives = 21/29 (72.41%), Query Frame = 2

Query:  302 LAEHRFLFLGAGEAGTGIAELIALEISKQ 388
            L+E + +FLGAG AG GIAE+I  +  ++
Sbjct:  293 LSEKKIVFLGAGSAGCGIAEMIIAQTQRE 321

HSP 3 Score: 33.113 bits (74), Expect = 5.691e-16
Identity = 16/28 (57.14%), Postives = 17/28 (60.71%), Query Frame = 3

Query:  165 FEDFANHNAFELLAKYGTTHLVFNDDIQ 248
            FEDFA  NA  LL +Y      FNDDIQ
Sbjct:  245 FEDFAQKNAMPLLNRYRNEICSFNDDIQ 272

[Close]

BLAST of CF509642 vs. ExPASy Swiss-Prot
Match: MAO1_ECOUT (NAD-dependent malic enzyme OS=Escherichia coli (strain UTI89 / UPEC) GN=sfcA PE=3 SV=2)

HSP 1 Score: 57.3806 bits (137), Expect = 5.691e-16
Identity = 26/51 (50.98%), Postives = 35/51 (68.63%), Query Frame = 1

Query:    1 ITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVL 153
            + +DVGTNN+QLL D  Y+G R  R T  EY E +DEF+ AVKQ + + +L
Sbjct:  193 VVLDVGTNNQQLLNDPLYMGWRNPRITDDEYYEFVDEFIQAVKQRWPDVLL 243

HSP 2 Score: 33.4982 bits (75), Expect = 5.691e-16
Identity = 14/29 (48.28%), Postives = 21/29 (72.41%), Query Frame = 2

Query:  302 LAEHRFLFLGAGEAGTGIAELIALEISKQ 388
            L+E + +FLGAG AG GIAE+I  +  ++
Sbjct:  293 LSEKKIVFLGAGSAGCGIAEMIIAQTQRE 321

HSP 3 Score: 33.113 bits (74), Expect = 5.691e-16
Identity = 16/28 (57.14%), Postives = 17/28 (60.71%), Query Frame = 3

Query:  165 FEDFANHNAFELLAKYGTTHLVFNDDIQ 248
            FEDFA  NA  LL +Y      FNDDIQ
Sbjct:  245 FEDFAQKNAMPLLNRYRNEICSFNDDIQ 272

[Close]

BLAST of CF509642 vs. ExPASy Swiss-Prot
Match: MAO1_ECOSM (NAD-dependent malic enzyme OS=Escherichia coli (strain SMS-3-5 / SECEC) GN=sfcA PE=3 SV=1)

HSP 1 Score: 57.3806 bits (137), Expect = 5.691e-16
Identity = 26/51 (50.98%), Postives = 35/51 (68.63%), Query Frame = 1

Query:    1 ITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVL 153
            + +DVGTNN+QLL D  Y+G R  R T  EY E +DEF+ AVKQ + + +L
Sbjct:  193 VVLDVGTNNQQLLNDPLYMGWRNPRITDDEYYEFVDEFIQAVKQRWPDVLL 243

HSP 2 Score: 33.4982 bits (75), Expect = 5.691e-16
Identity = 14/29 (48.28%), Postives = 21/29 (72.41%), Query Frame = 2

Query:  302 LAEHRFLFLGAGEAGTGIAELIALEISKQ 388
            L+E + +FLGAG AG GIAE+I  +  ++
Sbjct:  293 LSEKKIVFLGAGSAGCGIAEMIIAQTQRE 321

HSP 3 Score: 33.113 bits (74), Expect = 5.691e-16
Identity = 16/28 (57.14%), Postives = 17/28 (60.71%), Query Frame = 3

Query:  165 FEDFANHNAFELLAKYGTTHLVFNDDIQ 248
            FEDFA  NA  LL +Y      FNDDIQ
Sbjct:  245 FEDFAQKNAMPLLNRYRNEICSFNDDIQ 272

[Close]

BLAST of CF509642 vs. ExPASy Swiss-Prot
Match: MAO1_ECOLU (NAD-dependent malic enzyme OS=Escherichia coli O17:K52:H18 (strain UMN026 / ExPEC) GN=sfcA PE=3 SV=1)

HSP 1 Score: 57.3806 bits (137), Expect = 5.691e-16
Identity = 26/51 (50.98%), Postives = 35/51 (68.63%), Query Frame = 1

Query:    1 ITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVL 153
            + +DVGTNN+QLL D  Y+G R  R T  EY E +DEF+ AVKQ + + +L
Sbjct:  193 VVLDVGTNNQQLLNDPLYMGWRNPRITDDEYYEFVDEFIQAVKQRWPDVLL 243

HSP 2 Score: 33.4982 bits (75), Expect = 5.691e-16
Identity = 14/29 (48.28%), Postives = 21/29 (72.41%), Query Frame = 2

Query:  302 LAEHRFLFLGAGEAGTGIAELIALEISKQ 388
            L+E + +FLGAG AG GIAE+I  +  ++
Sbjct:  293 LSEKKIVFLGAGSAGCGIAEMIIAQTQRE 321

HSP 3 Score: 33.113 bits (74), Expect = 5.691e-16
Identity = 16/28 (57.14%), Postives = 17/28 (60.71%), Query Frame = 3

Query:  165 FEDFANHNAFELLAKYGTTHLVFNDDIQ 248
            FEDFA  NA  LL +Y      FNDDIQ
Sbjct:  245 FEDFAQKNAMPLLNRYRNEICSFNDDIQ 272

[Close]

BLAST of CF509642 vs. ExPASy Swiss-Prot
Match: MAO1_ECOLC (NAD-dependent malic enzyme OS=Escherichia coli (strain ATCC 8739 / DSM 1576 / Crooks) GN=sfcA PE=3 SV=1)

HSP 1 Score: 57.3806 bits (137), Expect = 5.691e-16
Identity = 26/51 (50.98%), Postives = 35/51 (68.63%), Query Frame = 1

Query:    1 ITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVL 153
            + +DVGTNN+QLL D  Y+G R  R T  EY E +DEF+ AVKQ + + +L
Sbjct:  193 VVLDVGTNNQQLLNDPLYMGWRNPRITDDEYYEFVDEFIQAVKQRWPDVLL 243

HSP 2 Score: 33.4982 bits (75), Expect = 5.691e-16
Identity = 14/29 (48.28%), Postives = 21/29 (72.41%), Query Frame = 2

Query:  302 LAEHRFLFLGAGEAGTGIAELIALEISKQ 388
            L+E + +FLGAG AG GIAE+I  +  ++
Sbjct:  293 LSEKKIVFLGAGSAGCGIAEMIIAQTQRE 321

HSP 3 Score: 33.113 bits (74), Expect = 5.691e-16
Identity = 16/28 (57.14%), Postives = 17/28 (60.71%), Query Frame = 3

Query:  165 FEDFANHNAFELLAKYGTTHLVFNDDIQ 248
            FEDFA  NA  LL +Y      FNDDIQ
Sbjct:  245 FEDFAQKNAMPLLNRYRNEICSFNDDIQ 272

The following BLAST results are available for this feature:

BLAST of CF509642 vs. ExPASy Swiss-Prot

BLAST of CF509642 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 133


Match Name	E-value	Identity	Description
MAO1_AERS4	1.566e-16	50.98	NAD-dependent malic enzyme OS=Aeromonas salmonicid...	[more]
MAO1_AERHH	1.566e-16	50.98	NAD-dependent malic enzyme OS=Aeromonas hydrophila...	[more]
MAO1_SHISS	4.397e-16	50.98	NAD-dependent malic enzyme OS=Shigella sonnei (str...	[more]
MAO1_SHIFL	5.691e-16	50.98	NAD-dependent malic enzyme OS=Shigella flexneri GN...	[more]
MAO1_SHIF8	5.691e-16	50.98	NAD-dependent malic enzyme OS=Shigella flexneri se...	[more]
MAO1_ESCF3	5.691e-16	50.98	NAD-dependent malic enzyme OS=Escherichia ferguson...	[more]
MAO1_ECOUT	5.691e-16	50.98	NAD-dependent malic enzyme OS=Escherichia coli (st...	[more]
MAO1_ECOSM	5.691e-16	50.98	NAD-dependent malic enzyme OS=Escherichia coli (st...	[more]
MAO1_ECOLU	5.691e-16	50.98	NAD-dependent malic enzyme OS=Escherichia coli O17...	[more]
MAO1_ECOLC	5.691e-16	50.98	NAD-dependent malic enzyme OS=Escherichia coli (st...	[more]

Pages

Properties


Property Name	Value
Genbank description	USDA-FP_125000-150 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-7_F07 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CF509642 ID=CF509642; Name=CF509642; organism=Citrus sinensis; type=EST; length=433bp
ATAACCGTTGATGTGGGTACAAACAATGAGCAATTGTTGAAGGATGAATT
CTACATTGGTCTACGACAGAGGAGGGCAACCGGACAGGAATATGCTGAAC
TTCTAGATGAATTCATGAGTGCTGTCAAGCAAAATTATGGAGAAAAAGTT
CTTATACAGGTTAGTTTGAGGATTTCGCAAACCACAATGCTTTTGAGTTG
CTTGCAAAATATGGCACAACTCATCTTGTCTTCAATGATGATATACAGCG
TCTGTTGTTCTTGCTGGGGTAGTTGCAGCACTGAAGTTGATTGGTGGCAC
ACTGGCCGAGCATAGATTTTTGTTTCTTGGAGCTGGGGAGGCTGGGACTG
GTATAGCTGAACTTATTGCGCTTGAGATATCAAAGCAGACTAAAGCTCCT
GTGGAAGAGACCCCGGAAGAAGATATGTCTTGT