CF832325

Overview
NameCF832325
Unique NameCF832325
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length709
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig1234 contig Csv1_Contig1234:162..870. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CF832325 vs. ExPASy Swiss-Prot
Match: METK3_PICSI (S-adenosylmethionine synthetase 3 OS=Picea sitchensis GN=METK3 PE=2 SV=1)

HSP 1 Score: 393.66 bits (1010), Expect = 5.231e-109
Identity = 188/207 (90.82%), Postives = 197/207 (95.17%), Query Frame = 3 
Query:   87 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETPEFMPLSHVLATKLGARLTEVRKNSTCSWLRPDGKTQVTVEYYNDNGAMVPVRVHTVLISTQHDETVTNDEIA 707
            METFLFTSESVNEGHPDKLCDQISDAVLDACL QDP+SKVACETCTKTNMVMVFGEITTKA VDYE+IVR TCR IGF+SDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPE+IGAG QGHMFGYATDETPE MPL+HVLATKLGA+LTEVRKN TC+WLRPDGKTQVT+EY ND GAMVP RVHTVLISTQHDETVTND+IA
Sbjct:    1 METFLFTSESVNEGHPDKLCDQISDAVLDACLTQDPDSKVACETCTKTNMVMVFGEITTKADVDYEQIVRKTCREIGFISDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEEIGAGYQGHMFGYATDETPELMPLTHVLATKLGAKLTEVRKNGTCAWLRPDGKTQVTIEYRNDGGAMVPERVHTVLISTQHDETVTNDQIA 207
[Close]
BLAST of CF832325 vs. ExPASy Swiss-Prot
Match: METK2_PICSI (S-adenosylmethionine synthetase 2 OS=Picea sitchensis GN=METK2 PE=2 SV=1)

HSP 1 Score: 393.66 bits (1010), Expect = 5.231e-109
Identity = 187/207 (90.34%), Postives = 197/207 (95.17%), Query Frame = 3 
Query:   87 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETPEFMPLSHVLATKLGARLTEVRKNSTCSWLRPDGKTQVTVEYYNDNGAMVPVRVHTVLISTQHDETVTNDEIA 707
            METFLFTSESVNEGHPDKLCDQISDAVLDACL QDP+SKVACETCTKTNMVMVFGEITTKA +DYE+IVR TCR IGF+SDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPE+IGAGDQGHMFGYATDETPE MPL+HVLATKLGA+LTEVRKN TC WLRPDGKTQVT+EY N+ GAMVP RVHTVLISTQHDETVTND+IA
Sbjct:    1 METFLFTSESVNEGHPDKLCDQISDAVLDACLIQDPDSKVACETCTKTNMVMVFGEITTKADIDYEQIVRKTCREIGFISDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEEIGAGDQGHMFGYATDETPELMPLTHVLATKLGAKLTEVRKNGTCPWLRPDGKTQVTIEYRNEGGAMVPERVHTVLISTQHDETVTNDQIA 207
[Close]
BLAST of CF832325 vs. ExPASy Swiss-Prot
Match: METK1_PICSI (S-adenosylmethionine synthetase 1 OS=Picea sitchensis GN=METK1 PE=2 SV=1)

HSP 1 Score: 393.66 bits (1010), Expect = 5.231e-109
Identity = 187/207 (90.34%), Postives = 198/207 (95.65%), Query Frame = 3 
Query:   87 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETPEFMPLSHVLATKLGARLTEVRKNSTCSWLRPDGKTQVTVEYYNDNGAMVPVRVHTVLISTQHDETVTNDEIA 707
            M+TFLFTSESVNEGHPDKLCDQISDA+LDACLEQDPESKVACETCTKTNMVM+FGEITTKA +DYEKIVRDTCR IGFVS DVGLDAD+CKVLVNIEQQSPDIAQGVHGH TK+PE+IGAGDQGHMFGYATDETPE MPL+HVLATKLGARLTEVRK+ TC WLRPDGKTQVTVEY N+ GAMVP+RVHTVLISTQHDETVTNDEIA
Sbjct:    1 MDTFLFTSESVNEGHPDKLCDQISDAILDACLEQDPESKVACETCTKTNMVMIFGEITTKANIDYEKIVRDTCRGIGFVSADVGLDADNCKVLVNIEQQSPDIAQGVHGHLTKKPEEIGAGDQGHMFGYATDETPELMPLTHVLATKLGARLTEVRKDGTCPWLRPDGKTQVTVEYKNEGGAMVPLRVHTVLISTQHDETVTNDEIA 207
[Close]
BLAST of CF832325 vs. ExPASy Swiss-Prot
Match: METK3_VITVI (S-adenosylmethionine synthetase 3 OS=Vitis vinifera GN=METK3 PE=3 SV=1)

HSP 1 Score: 393.275 bits (1009), Expect = 6.832e-109
Identity = 186/207 (89.86%), Postives = 199/207 (96.14%), Query Frame = 3 
Query:   87 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETPEFMPLSHVLATKLGARLTEVRKNSTCSWLRPDGKTQVTVEYYNDNGAMVPVRVHTVLISTQHDETVTNDEIA 707
            M+TFLFTSESVNEGHPDKLCDQ+SDA+LDACLEQDPESKVACETC+KTNMVMVFGEITTKAKVDYEKIVRDTCR IGFVS DVGLDAD+CKVLVNIEQQSPDIAQGVHGH +K+PE+IGAGDQGHMFGYATDETPE MPL+HVLATKLGA+LTEVRKN TC WLRPDGKTQVTVEY N+ GAMVP+RVHTVLISTQHDETVTND+IA
Sbjct:    1 MDTFLFTSESVNEGHPDKLCDQVSDAILDACLEQDPESKVACETCSKTNMVMVFGEITTKAKVDYEKIVRDTCRGIGFVSADVGLDADNCKVLVNIEQQSPDIAQGVHGHLSKKPEEIGAGDQGHMFGYATDETPELMPLTHVLATKLGAKLTEVRKNKTCPWLRPDGKTQVTVEYRNEGGAMVPIRVHTVLISTQHDETVTNDQIA 207
[Close]
BLAST of CF832325 vs. ExPASy Swiss-Prot
Match: METK2_ACTCH (S-adenosylmethionine synthetase 2 OS=Actinidia chinensis GN=SAM2 PE=2 SV=1)

HSP 1 Score: 393.275 bits (1009), Expect = 6.832e-109
Identity = 186/207 (89.86%), Postives = 199/207 (96.14%), Query Frame = 3 
Query:   87 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETPEFMPLSHVLATKLGARLTEVRKNSTCSWLRPDGKTQVTVEYYNDNGAMVPVRVHTVLISTQHDETVTNDEIA 707
            ME+FLFTSESVNEGHPDKLCDQ+SDA+LDACLEQDPESKVACETCTKTNMVMVFGEITTKAKV+YEKIVRDTCR IGF S DVGLDADHCKVLVNIEQQSPDIAQGVHGH +K+PE+IGAGDQGHMFGYATDETPE MPL+HVLATKLGA+LTEVRKN+TC WLRPDGKTQVTVEY N+ GAMVPVRVHTVLISTQHDETVTN++IA
Sbjct:    1 MESFLFTSESVNEGHPDKLCDQVSDAILDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVNYEKIVRDTCRGIGFTSPDVGLDADHCKVLVNIEQQSPDIAQGVHGHLSKKPEEIGAGDQGHMFGYATDETPELMPLTHVLATKLGAKLTEVRKNNTCPWLRPDGKTQVTVEYRNEGGAMVPVRVHTVLISTQHDETVTNEQIA 207
[Close]
BLAST of CF832325 vs. ExPASy Swiss-Prot
Match: METK1_ACTCH (S-adenosylmethionine synthetase 1 OS=Actinidia chinensis GN=SAM1 PE=2 SV=1)

HSP 1 Score: 392.889 bits (1008), Expect = 8.923e-109
Identity = 185/207 (89.37%), Postives = 199/207 (96.14%), Query Frame = 3 
Query:   87 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETPEFMPLSHVLATKLGARLTEVRKNSTCSWLRPDGKTQVTVEYYNDNGAMVPVRVHTVLISTQHDETVTNDEIA 707
            M+TFLFTSESVNEGHPDKLCDQ+SDA+LDACL+QDPESKVACE+CTKTNMVMVFGEITTKA+V+YEKIVRDTCR IGF S DVGLDADHCKVLVNIEQQSPDIAQGVHGH TK+PE+IGAGDQGHMFGYATDETPE MPL+HVLATKLGA+LTEVRKNSTC WLRPDGKTQVTVEY N+ GAMVP+RVHTVLISTQHDETVTND+IA
Sbjct:    1 MDTFLFTSESVNEGHPDKLCDQVSDAILDACLKQDPESKVACESCTKTNMVMVFGEITTKAQVNYEKIVRDTCRGIGFTSPDVGLDADHCKVLVNIEQQSPDIAQGVHGHLTKKPEEIGAGDQGHMFGYATDETPELMPLTHVLATKLGAKLTEVRKNSTCPWLRPDGKTQVTVEYRNEGGAMVPIRVHTVLISTQHDETVTNDQIA 207
[Close]
BLAST of CF832325 vs. ExPASy Swiss-Prot
Match: METK4_BRAJU (S-adenosylmethionine synthetase 4 OS=Brassica juncea GN=MSAMS4 PE=2 SV=1)

HSP 1 Score: 392.504 bits (1007), Expect = 1.165e-108
Identity = 186/207 (89.86%), Postives = 195/207 (94.20%), Query Frame = 3 
Query:   87 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETPEFMPLSHVLATKLGARLTEVRKNSTCSWLRPDGKTQVTVEYYNDNGAMVPVRVHTVLISTQHDETVTNDEIA 707
            METFLFTSESVNEGHPDKLCDQ+SDA+LDACLEQDPESKVACETCTKTNMVMVFGEITT AKVDYEKIVR TCR IGF+S DVGLDAD C VLVNIEQQSPDIAQGVHGH TK+PEDIGAGDQGHMFGYATDETPE MPL+HVLATKLGA+LTEVRKN TC WLRPDGKTQVTVEY N+ GAM+P+RVHTVLISTQHDETVTNDEIA
Sbjct:    1 METFLFTSESVNEGHPDKLCDQVSDAILDACLEQDPESKVACETCTKTNMVMVFGEITTSAKVDYEKIVRSTCREIGFISADVGLDADKCNVLVNIEQQSPDIAQGVHGHLTKKPEDIGAGDQGHMFGYATDETPELMPLTHVLATKLGAKLTEVRKNKTCPWLRPDGKTQVTVEYKNEGGAMIPIRVHTVLISTQHDETVTNDEIA 207
[Close]
BLAST of CF832325 vs. ExPASy Swiss-Prot
Match: METK1_VITVI (S-adenosylmethionine synthetase 1 OS=Vitis vinifera GN=METK1 PE=3 SV=1)

HSP 1 Score: 392.504 bits (1007), Expect = 1.165e-108
Identity = 185/207 (89.37%), Postives = 199/207 (96.14%), Query Frame = 3 
Query:   87 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETPEFMPLSHVLATKLGARLTEVRKNSTCSWLRPDGKTQVTVEYYNDNGAMVPVRVHTVLISTQHDETVTNDEIA 707
            M+TFLFTSESVNEGHPDKLCDQ+SDA+LDACLEQDPESKVACETCTKTNMVMVFGEITTKAKV+YEKIVRDTCR IGFVS DVGLDAD+CKVLVNIEQQSPDIAQGVHGH TK+PE+IGAGDQGHMFGYATDETPE MPL+HVLATKLGA+LTEVRKN TC WLRPDGKTQVTVEY N+ GAMVP+RVHTVL+STQHDETVTN++IA
Sbjct:    1 MDTFLFTSESVNEGHPDKLCDQVSDAILDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVNYEKIVRDTCRGIGFVSADVGLDADNCKVLVNIEQQSPDIAQGVHGHLTKKPEEIGAGDQGHMFGYATDETPELMPLTHVLATKLGAKLTEVRKNGTCPWLRPDGKTQVTVEYRNEGGAMVPIRVHTVLVSTQHDETVTNEQIA 207
[Close]
BLAST of CF832325 vs. ExPASy Swiss-Prot
Match: METK1_ATRNU (S-adenosylmethionine synthetase 1 OS=Atriplex nummularia GN=SAMS1 PE=1 SV=2)

HSP 1 Score: 392.504 bits (1007), Expect = 1.165e-108
Identity = 188/207 (90.82%), Postives = 197/207 (95.17%), Query Frame = 3 
Query:   87 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETPEFMPLSHVLATKLGARLTEVRKNSTCSWLRPDGKTQVTVEYYNDNGAMVPVRVHTVLISTQHDETVTNDEIA 707
            ++TFLFTSESVNEGHPDKLCDQISDAVLDACL QDPESKVACETCTKTN+VMVFGEITTKA VDYEKIVR TCR IGFVS DVGLDAD+CKVLV IEQQSPDIAQGVHGH ++RPE+IGAGDQGHMFGYATDETPE MPLSHVLATKLGARLTEVRKN TC WLRPDGKTQVTVEYYN+NGAMVP+RVHTVLISTQHDETVTNDEIA
Sbjct:    5 VDTFLFTSESVNEGHPDKLCDQISDAVLDACLAQDPESKVACETCTKTNLVMVFGEITTKANVDYEKIVRQTCRDIGFVSADVGLDADNCKVLVYIEQQSPDIAQGVHGHLSRRPEEIGAGDQGHMFGYATDETPELMPLSHVLATKLGARLTEVRKNGTCPWLRPDGKTQVTVEYYNENGAMVPIRVHTVLISTQHDETVTNDEIA 211
[Close]
BLAST of CF832325 vs. ExPASy Swiss-Prot
Match: METK4_ARATH (S-adenosylmethionine synthetase 4 OS=Arabidopsis thaliana GN=METK4 PE=1 SV=1)

HSP 1 Score: 391.734 bits (1005), Expect = 1.988e-108
Identity = 186/207 (89.86%), Postives = 198/207 (95.65%), Query Frame = 3 
Query:   87 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETPEFMPLSHVLATKLGARLTEVRKNSTCSWLRPDGKTQVTVEYYNDNGAMVPVRVHTVLISTQHDETVTNDEIA 707
            ME+FLFTSESVNEGHPDKLCDQISDA+LDACLEQDPESKVACETCTKTNMVMVFGEITTKA VDYE+IVR TCR IGFVS DVGLDAD+CKVLVNIEQQSPDIAQGVHGH TK+PE++GAGDQGHMFGYATDETPE MPL+HVLATKLGA+LTEVRKN TC WLRPDGKTQVT+EY N++GAMVPVRVHTVLISTQHDETVTNDEIA
Sbjct:    1 MESFLFTSESVNEGHPDKLCDQISDAILDACLEQDPESKVACETCTKTNMVMVFGEITTKANVDYEQIVRKTCREIGFVSADVGLDADNCKVLVNIEQQSPDIAQGVHGHLTKKPEEVGAGDQGHMFGYATDETPELMPLTHVLATKLGAKLTEVRKNGTCPWLRPDGKTQVTIEYINESGAMVPVRVHTVLISTQHDETVTNDEIA 207
The following BLAST results are available for this feature:
BLAST of CF832325 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
METK3_PICSI5.231e-10990.82S-adenosylmethionine synthetase 3 OS=Picea sitchen... [more]
METK2_PICSI5.231e-10990.34S-adenosylmethionine synthetase 2 OS=Picea sitchen... [more]
METK1_PICSI5.231e-10990.34S-adenosylmethionine synthetase 1 OS=Picea sitchen... [more]
METK3_VITVI6.832e-10989.86S-adenosylmethionine synthetase 3 OS=Vitis vinifer... [more]
METK2_ACTCH6.832e-10989.86S-adenosylmethionine synthetase 2 OS=Actinidia chi... [more]
METK1_ACTCH8.923e-10989.37S-adenosylmethionine synthetase 1 OS=Actinidia chi... [more]
METK4_BRAJU1.165e-10889.86S-adenosylmethionine synthetase 4 OS=Brassica junc... [more]
METK1_VITVI1.165e-10889.37S-adenosylmethionine synthetase 1 OS=Vitis vinifer... [more]
METK1_ATRNU1.165e-10890.82S-adenosylmethionine synthetase 1 OS=Atriplex numm... [more]
METK4_ARATH1.988e-10889.86S-adenosylmethionine synthetase 4 OS=Arabidopsis t... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS02_01K23_r Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa01K23, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF832325 ID=CF832325; Name=CF832325; organism=Citrus sinensis; type=EST; length=709bp
TTGAGTTCCGCTCTTTTATTGAGGTCTGGTCTTAAGTCTGAATCTTAGAT
CTGCGCCGAAACACTTGCCAGCCACAGCTTGATAAGATGGAGACCTTTCT
ATTCACCTCTGAATCTGTGAACGAGGGTCACCCCGACAAGTTGTGCGACC
AGATCTCTGATGCGGTGCTTGATGCCTGCCTTGAGCAGGATCCTGAGAGC
AAGGTTGCCTGTGAGACATGCACCAAGACCAACATGGTCATGGTCTTTGG
AGAGATTACCACCAAGGCCAAGGTAGACTATGAGAAGATTGTTCGCGACA
CTTGCCGTTCCATTGGATTTGTTTCTGATGATGTGGGTCTTGATGCCGAT
CACTGCAAAGTCCTGGTCAATATTGAGCAACAAAGCCCTGATATTGCCCA
GGGTGTTCATGGCCACTTTACAAAGCGCCCTGAGGACATTGGTGCTGGTG
ACCAGGGCCATATGTTTGGTTATGCTACTGATGAGACCCCTGAATTTATG
CCTTTGAGCCATGTCCTTGCCACCAAACTTGGTGCTCGTCTCACGGAGGT
TAGGAAGAATAGTACCTGCTCTTGGCTAAGGCCCGATGGGAAGACCCAGG
TCACTGTTGAGTACTACAATGACAATGGTGCCATGGTCCCAGTCCGTGTC
CACACGGTCCTCATTTCAACCCAGCATGATGAGACTGTCACAAATGATGA
AATTGCTGC
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