CF832451

Overview
NameCF832451
Unique NameCF832451
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length866
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CF832451 vs. ExPASy Swiss-Prot
Match: CP4F5_RAT (Cytochrome P450 4F5 OS=Rattus norvegicus GN=Cyp4f5 PE=2 SV=1)

HSP 1 Score: 98.9821 bits (245), Expect = 3.708e-20
Identity = 56/171 (32.75%), Postives = 94/171 (54.97%), Query Frame = -3
Query:  355 ECKLFYFAGQETTSVLLNWTMVLLSKHQDWQERARQEVLQVFGNNKP---NYDGLNHLKIVQMILYEVLRLYPPVTVLSRSVLKETKL-GNLTLPAGVQIGLPMILVHQDPELWGYDAVEFKPERFAEGISKAAKNQVSYFPFALGPRICVGQNFALVEAKMAMAMILQNY 855
            E   F F G +TT+  L+W +  L++H ++QER RQEV ++  + +P    +D L  L  + M + E LRL+PP   L R   ++  L     +P G    + +  +H +P +W  D   F P RF +  ++  ++ +S+ PF+ GPR C+GQ FA+ E K+ +A+ L  +
Sbjct:  323 EADTFMFGGHDTTASALSWILYNLARHPEYQERCRQEVWELLRDREPEEIEWDDLAQLPFLTMCIKESLRLHPPAIDLLRRCTQDIVLPDGRVIPKGNICVISIFGIHHNPSVWP-DPEVFDPFRF-DSENRQKRSPLSFIPFSAGPRNCIGQTFAMNEMKVVVALTLLRF 491          
BLAST of CF832451 vs. ExPASy Swiss-Prot
Match: CP4AC_RAT (Cytochrome P450 4A12 OS=Rattus norvegicus GN=Cyp4a12 PE=2 SV=2)

HSP 1 Score: 98.9821 bits (245), Expect = 3.708e-20
Identity = 64/203 (31.53%), Postives = 107/203 (52.71%), Query Frame = -3
Query:  259 ECKLFYFAGQETTSVLLNWTMVLLSKHQDWQERARQEVLQVFGNNKP-NYDGLNHLKIVQMILYEVLRLYPPVTVLSRSVLKETKLGN-LTLPAGVQIGLPMILVHQDPELWGYDAVEFKPERFAEGISKAAKNQVSYFPFALGPRICVGQNFALVEAKMAMAMILQNYSFELSPSYVHAPTAVP--TLHPELGTQLILRKLR 855
            E   F F G +TT+  ++W    L+ + + Q+  R+E+  + G+     +D L+ +    M + E LR+YPPVT +SR +       +  +LP G+ + L    +H +P +W    V F P RFA    +++++  S+ PF+ G R C+G+ FA+ E K+A+A+ L    FEL P     P  +P   L  + G  L L+KL+
Sbjct:  312 EVDTFMFEGHDTTASGISWIFYALATNPEHQQGCRKEIQSLLGDGASITWDDLDKMPYTTMCIKEALRIYPPVTAVSRMLSTPVTFPDGRSLPKGITVMLSFYGLHHNPTVWPNPEV-FDPYRFAP---ESSRHSHSFLPFSGGARNCIGKQFAMNELKVAVALTL--LRFELLPDPTRIPIPIPRLVLKSKNGIYLRLKKLQ 508          
BLAST of CF832451 vs. ExPASy Swiss-Prot
Match: CP4FN_HUMAN (Cytochrome P450 4F22 OS=Homo sapiens GN=CYP4F22 PE=2 SV=1)

HSP 1 Score: 98.5969 bits (244), Expect = 4.842e-20
Identity = 54/171 (31.58%), Postives = 97/171 (56.73%), Query Frame = -3
Query:  355 ECKLFYFAGQETTSVLLNWTMVLLSKHQDWQERARQEVLQVFGNNKPN---YDGLNHLKIVQMILYEVLRLYPPVTVLSRSVLKETKLGN-LTLPAGVQIGLPMILVHQDPELWGYDAVEFKPERFAEGISKAAKNQVSYFPFALGPRICVGQNFALVEAKMAMAMILQNY 855
            E   F F G +TTS  ++W +  L+K+ ++QE+ R+E+ +V    +     +D L  L    M + E LR YPPVT++SR   ++ KL +   +P G+   + +   H +P +W  D+  + P RF +  +   ++ ++Y PF+ GPR C+GQ+FA+ E ++ +A+ L  +
Sbjct:  328 EADTFMFEGHDTTSSGISWMLFNLAKYPEYQEKCREEIQEVMKGRELEELEWDDLTQLPFTTMCIKESLRQYPPVTLVSRQCTEDIKLPDGRIIPKGIICLVSIYGTHHNPTVWP-DSKVYNPYRF-DPDNPQQRSPLAYVPFSAGPRNCIGQSFAMAELRVVVALTLLRF 496          
BLAST of CF832451 vs. ExPASy Swiss-Prot
Match: CP4B1_RABIT (Cytochrome P450 4B1 OS=Oryctolagus cuniculus GN=CYP4B1 PE=1 SV=1)

HSP 1 Score: 98.5969 bits (244), Expect = 4.842e-20
Identity = 61/202 (30.20%), Postives = 107/202 (52.97%), Query Frame = -3
Query:  262 ECKLFYFAGQETTSVLLNWTMVLLSKHQDWQERARQEVLQVFGNNKP-NYDGLNHLKIVQMILYEVLRLYPPVTVLSRSVLKETK-LGNLTLPAGVQIGLPMILVHQDPELWGYDAVEFKPERFAEGISKAAKNQVSYFPFALGPRICVGQNFALVEAKMAMAMILQNYSFELSPSYVHAPTAVP--TLHPELGTQLILRKL 855
            E   F F G +TT+  ++W +  ++ + + Q+R R+EV ++ G+     ++ L  +  + M + E  RLYPPV  + R + K    +   +LPAG  I L +  +H++ ++W  D   F P RF+   S + ++  ++ PF+ GPR C+GQ FA+ E K+  A+ L  + F + P  +  P  +P   L  + G  L L+ L
Sbjct:  303 EVDTFMFEGHDTTTSGISWFLYCMALYPEHQQRCREEVREILGDQDSFQWEDLAKMTYLTMCMKECFRLYPPVPQVYRQLSKPVSFVDGRSLPAGSLISLHIYALHRNSDVWP-DPEVFDPLRFSPENS-SGRHPYAFIPFSAGPRNCIGQQFAMNEMKVVTALCLLRFEFSVDP--LRLPIKLPQLVLRSKNGIHLYLKPL 500          
BLAST of CF832451 vs. ExPASy Swiss-Prot
Match: CP4B1_MOUSE (Cytochrome P450 4B1 OS=Mus musculus GN=Cyp4b1 PE=1 SV=1)

HSP 1 Score: 98.5969 bits (244), Expect = 4.842e-20
Identity = 61/200 (30.50%), Postives = 102/200 (51.00%), Query Frame = -3
Query:  262 ECKLFYFAGQETTSVLLNWTMVLLSKHQDWQERARQEVLQVFGNNKP-NYDGLNHLKIVQMILYEVLRLYPPVTVLSRSVLKE-TKLGNLTLPAGVQIGLPMILVHQDPELWGYDAVEFKPERFAEGISKAAKNQVSYFPFALGPRICVGQNFALVEAKMAMAMILQNYSFELSPSYVHAPTAVPTLHPELGTQLILRKL 855
            E   F F G +TT+  ++W +  ++ +   Q+R R+EV ++ G+     +D L  +  + M + E  RLYPPV  + R + K  T +   +LPAG  I L +  +H++  +W  D   F P RF+   +   ++  ++ PF+ GPR C+GQ FA+ E K+  A+ L  + F   PS +        L  + G  L L+ L
Sbjct:  308 EVDTFMFEGHDTTTSGISWFLYCMALYPMHQQRCREEVREILGDRDSFQWDDLAQMTYLTMCMKECFRLYPPVPQVYRQLSKPVTFVDGRSLPAGSLISLHIYALHRNSAVWP-DPEVFDPLRFSPE-NMTGRHPFAFMPFSAGPRNCIGQQFAMNEMKVVTALCLLRFEFSPDPSKIPIKVPQLILRSKNGIHLYLKPL 505          
BLAST of CF832451 vs. ExPASy Swiss-Prot
Match: CP132_MYCTU (Putative cytochrome P450 132 OS=Mycobacterium tuberculosis GN=cyp132 PE=3 SV=1)

HSP 1 Score: 98.5969 bits (244), Expect = 4.842e-20
Identity = 59/200 (29.50%), Postives = 99/200 (49.50%), Query Frame = -3
Query:  265 VIEECKLFYFAGQETTSVLLNWTMVLLSKHQDWQERARQEVLQVFGNNKPNYDGLNHLKIVQMILYEVLRLYPPVTVLSRSVLKETKLGNLTLPAGVQIGLPMILVHQDPELWGYDAVEFKPERFAEGISKAAKNQVSYFPFALGPRICVGQNFALVEAKMAMAMILQNYSFELSPSYVHAPTAVPTLHPELGTQLILRK 864
            V +E   F  AG ETT+  ++W   L++ +   ++    E+  V G  +P  D L  L      L E  R +  V +++R  + +  +    +  G  + +P+  +H DP  W  D   F P RF        + + +Y PF  G RIC+GQ+FAL+E  +  A++ Q+++F+L+P Y     A  TL P+ G  +I R+
Sbjct:  264 VRDEALTFMLAGHETTANAMSWFWYLMALNPQARDHMLTELDDVLGMRRPTADDLGKLAWTTACLQESQRYFSSVWIIAREAVDDDIIDGHRIRRGTTVVIPIHHIHHDPRWWP-DPDRFDPGRFLR--CPTDRPRCAYLPFGGGRRICIGQSFALMEMVLMAAIMSQHFTFDLAPGYHVELEATLTLRPKHGVHVIGRR 460          
BLAST of CF832451 vs. ExPASy Swiss-Prot
Match: CP132_MYCBO (Putative cytochrome P450 132 OS=Mycobacterium bovis GN=cyp132 PE=3 SV=1)

HSP 1 Score: 98.5969 bits (244), Expect = 4.842e-20
Identity = 59/200 (29.50%), Postives = 99/200 (49.50%), Query Frame = -3
Query:  265 VIEECKLFYFAGQETTSVLLNWTMVLLSKHQDWQERARQEVLQVFGNNKPNYDGLNHLKIVQMILYEVLRLYPPVTVLSRSVLKETKLGNLTLPAGVQIGLPMILVHQDPELWGYDAVEFKPERFAEGISKAAKNQVSYFPFALGPRICVGQNFALVEAKMAMAMILQNYSFELSPSYVHAPTAVPTLHPELGTQLILRK 864
            V +E   F  AG ETT+  ++W   L++ +   ++    E+  V G  +P  D L  L      L E  R +  V +++R  + +  +    +  G  + +P+  +H DP  W  D   F P RF        + + +Y PF  G RIC+GQ+FAL+E  +  A++ Q+++F+L+P Y     A  TL P+ G  +I R+
Sbjct:  264 VRDEALTFMLAGHETTANAMSWFWYLMALNPQARDHMLTELDDVLGMRRPTADDLGKLAWTTACLQESQRYFSSVWIIAREAVDDDIIDGHRIRRGTTVVIPIHHIHHDPRWWP-DPDRFDPGRFLR--CPTDRPRCAYLPFGGGRRICIGQSFALMEMVLMAAIMSQHFTFDLAPGYHVELEATLTLRPKHGVHVIGRR 460          
BLAST of CF832451 vs. ExPASy Swiss-Prot
Match: CP9F2_DROME (Probable cytochrome P450 9f2 OS=Drosophila melanogaster GN=Cyp9f2 PE=2 SV=1)

HSP 1 Score: 98.2117 bits (243), Expect = 6.324e-20
Identity = 59/179 (32.96%), Postives = 101/179 (56.42%), Query Frame = -3
Query:  349 VIEECKLFYFAGQETTSVLLNWTMVLLSKHQDWQERARQEVLQV---FGNNKPNYDGLNHLKIVQMILYEVLRLYPPVTVLSRSVLKETKLG----NLTLPAGVQIGLPMILVHQDPELWGYDAVEFKPERFAEGISKAAKNQVSYFPFALGPRICVGQNFALVEAKMAMAMILQNYSF 864
            ++ +C +F+FAG ET++VL+ +T   L ++QD Q+R  +EV QV       +  Y+ +  +K +  ++ EVLR +P    + R   K+         + +  G  I LP    H+DP+ +  + ++F PERF++  +K +    +YFPF LG R C+G  FAL+EAK  +  +L++Y F
Sbjct:  307 IVAQCFVFFFAGFETSAVLMCFTAHELMENQDVQQRLYEEVQQVDQDLEGKELTYEAIMGMKYLDQVVNEVLRKWPAAIAVDRECNKDITFDVDGQKVEVKKGDVIWLPTCGFHRDPKYFE-NPMKFDPERFSDE-NKESIQPFTYFPFGLGQRNCIGSRFALLEAKAVIYYLLKDYRF 483          
BLAST of CF832451 vs. ExPASy Swiss-Prot
Match: CP6W1_DROME (Probable cytochrome P450 6w1 OS=Drosophila melanogaster GN=Cyp6w1 PE=2 SV=1)

HSP 1 Score: 98.2117 bits (243), Expect = 6.324e-20
Identity = 55/196 (28.06%), Postives = 101/196 (51.53%), Query Frame = -3
Query:  304 VIEECKLFYFAGQETTSVLLNWTMVLLSKHQDWQERARQEVLQVFGNNKP-NYDGLNHLKIVQMILYEVLRLYPPVTVLSRSVLKETK--------LGNLTLPAGVQIGLPMILVHQDPELWGYDAVEFKPERFAEGISKAAKNQVSYFPFALGPRICVGQNFALVEAKMAMAMILQNYSFELSPSYVHAPTAVPT 864
            ++ +  +F  AG ET++  +  T+  L+K++  Q+R RQE++  FG+    +Y+ +  +  +  ++ E LR YP V  + R   +  +          N+ LP G+ I +  + VH+DP+ W  D  ++ PERF    ++   N  +Y PF +GPR C+G    L+++K+ +  IL+N+ F      +      PT
Sbjct:  295 LVAQAAVFQTAGFETSASTMTMTLYELAKNEALQDRLRQEIVDFFGDEDHISYERIQEMPYLSQVVNETLRKYPIVGYIERECSQPAEGERFTLEPFHNMELPHGMSIYMSTVAVHRDPQYWP-DPEKYDPERFNSS-NRDNLNMDAYMPFGVGPRNCIGMRLGLLQSKLGLVHILRNHRFHTCDKTIKKIEWAPT 488          
BLAST of CF832451 vs. ExPASy Swiss-Prot
Match: CP4B1_HUMAN (Cytochrome P450 4B1 OS=Homo sapiens GN=CYP4B1 PE=1 SV=2)

HSP 1 Score: 98.2117 bits (243), Expect = 6.324e-20
Identity = 64/201 (31.84%), Postives = 104/201 (51.74%), Query Frame = -3
Query:  262 ECKLFYFAGQETTSVLLNWTMVLLSKHQDWQERARQEVLQVFGNNKP-NYDGLNHLKIVQMILYEVLRLYPPVTVLSRSVLKE-TKLGNLTLPAGVQIGLPMILVHQDPELWGYDAVEFKPERFA-EGISKAAKNQVSYFPFALGPRICVGQNFALVEAKMAMAMILQNYSFELSPSYVHAPTAVPTLHPELGTQLILRKL 855
            E   F F G +TT+  ++W +  ++ + + Q R R+EV ++ G+     +D L  +  + M + E  RLYPPV  + R + K  T +   +LPAG  I + +  +H++  +W  D   F   RF+ E  SK  ++  ++ PF+ GPR C+GQ FA+ E K+  AM L  + F L PS +        L  + G  L L+ L
Sbjct:  308 EVDTFMFEGHDTTTSGISWFLYCMALYPEHQHRCREEVREILGDQDFFQWDDLGKMTYLTMCIKESFRLYPPVPQVYRQLSKPVTFVDGRSLPAGSLISMHIYALHRNSAVWP-DPEVFDSLRFSTENASK--RHPFAFMPFSAGPRNCIGQQFAMSEMKVVTAMCLLRFEFSLDPSRLPIKMPQLVLRSKNGFHLHLKPL 505          
The following BLAST results are available for this feature:
BLAST of CF832451 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 457
Match NameE-valueIdentityDescription
CP4F5_RAT3.708e-2032.75Cytochrome P450 4F5 OS=Rattus norvegicus GN=Cyp4f5... [more]
CP4AC_RAT3.708e-2031.53Cytochrome P450 4A12 OS=Rattus norvegicus GN=Cyp4a... [more]
CP4FN_HUMAN4.842e-2031.58Cytochrome P450 4F22 OS=Homo sapiens GN=CYP4F22 PE... [more]
CP4B1_RABIT4.842e-2030.20Cytochrome P450 4B1 OS=Oryctolagus cuniculus GN=CY... [more]
CP4B1_MOUSE4.842e-2030.50Cytochrome P450 4B1 OS=Mus musculus GN=Cyp4b1 PE=1... [more]
CP132_MYCTU4.842e-2029.50Putative cytochrome P450 132 OS=Mycobacterium tube... [more]
CP132_MYCBO4.842e-2029.50Putative cytochrome P450 132 OS=Mycobacterium bovi... [more]
CP9F2_DROME6.324e-2032.96Probable cytochrome P450 9f2 OS=Drosophila melanog... [more]
CP6W1_DROME6.324e-2028.06Probable cytochrome P450 6w1 OS=Drosophila melanog... [more]
CP4B1_HUMAN6.324e-2031.84Cytochrome P450 4B1 OS=Homo sapiens GN=CYP4B1 PE=1... [more]

Pages

back to top
Properties
Property NameValue
Genbank descriptionUCRCS02_01O03_f Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa01O03, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF832451 ID=CF832451; Name=CF832451; organism=Citrus sinensis; type=EST; length=866bp
TTTTTTTTTTTTTTACCCAGCATTAATGCTCTAATTCTAATCATGGTATT
ATAAAATTTTCCAATGTGGGGAAACATCTTTACTCTAAATAAAACTAGAG
TTGTGTTGAGCTCAATTTAAAAATCTAAACAGCTCTTGTCTAAGCTTTGA
ATCTCAATACAAAATTAAATACATTAAACTTCATTATAAAAGAGTCTACA
TTGGCATCATGCTAATCTTATTGACAATATATAGTTGCCGGTATTAATTA
TTTTTGCACCTTAGTTTCCGTAGAATCAATTGGGTACCCAGTTCCGGATG
AAGGGTTGGAACCGCCGTTGGAGCATGGACATAGGATGGAGACAACTCGA
ATGAGTAGTTTTGAAGAATCATTGCCATGGCCATTTTTGCTTCTACTAGA
GCAAAATTTTGTCCAACACATATCCTAGGACCCAATGCGAATGGGAAATA
TGAAACTTGATTTTTGGCCGCCTTTGAAATTCCTTCCGCAAACCTTTCCG
GCTTAAACTCAACTGCATCGTAACCCCAAAGTTCAGGATCCTGGTGAACT
AGGATCATTGGCAAGCCAATTTGAACCCCAGCGGGTAATGTCAAGTTTCC
GAGTTTCGTTTCCTTAAGAACAGATCGACTAAGCACAGTTACAGGCGGAT
AAAGCCTGAGTACCTCGTAAAGTATCATCTGGACAATTTTGAGGTGATTC
AATCCATCATAGTTGGGCTTATTATTGCCAAACACTTGCAAAACCTCTTG
CCTTGCACGTTCTTGCCAATCTTGATGCTTGCTCAATAAGACCATCGTCC
AATTAAGCAAAACAGAGGTAGTCTCTTGGCCGGCAAAGTAAAATAGCTTA
CACTCTTCGATCACAT
back to top