CF832451

Overview
NameCF832451
Unique NameCF832451
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length866
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CF832451 vs. ExPASy Swiss-Prot
Match: C99A2_ORYSJ (Cytochrome P450 99A2 OS=Oryza sativa subsp. japonica GN=CYP99A2 PE=2 SV=2)

HSP 1 Score: 90.5077 bits (223), Expect = 1.319e-17
Identity = 52/169 (30.77%), Postives = 93/169 (55.03%), Query Frame = -3
Query:  343 YFAGQETTSVLLNWTMVLLSKHQDWQERARQEVLQVFGNNKP-NYDGL-NHLKIVQMILYEVLRLYPPVTVLSRSVLKET-KLGNLTLPAGVQIGLPMILVHQDPELWGYDAVEFKPERFAEGISKAAKNQVSYFPFALGPRICVGQNFALVEAKMAMAMILQNYSFEL 840
            +  G ETTS    W M  L ++ +   + + EV +VF N  P +++GL ++L+ ++M++ E +RL P + +L   + +ET  +G   +  G ++ +    + + PE W  DA EFKPERF +G++    ++  Y PF  G R C G  F +V  ++ +A +L  + + L
Sbjct:  326 FTGGTETTSSAAEWVMSELMRNPEVMAKVQAEVRRVFDNKSPQDHEGLIDNLRYMKMVIKETMRLNPVLPLLMPHLCRETCDIGGYEVVEGTRVVINSWAMARSPEYWD-DAEEFKPERFEDGMADYKGSRFEYLPFGTGRRRCPGDTFGMVLLELIVARLLYYFDWSL 493          
BLAST of CF832451 vs. ExPASy Swiss-Prot
Match: CP2A5_MOUSE (Cytochrome P450 2A5 OS=Mus musculus GN=Cyp2a5 PE=2 SV=1)

HSP 1 Score: 90.1225 bits (222), Expect = 1.722e-17
Identity = 55/190 (28.95%), Postives = 99/190 (52.11%), Query Frame = -3
Query:  304 YFAGQETTSVLLNWTMVLLSKHQDWQERARQEVLQVFGNNK-PNYDGLNHLKIVQMILYEVLRLYPPVTV-LSRSVLKETKLGNLTLPAGVQIGLPMILVHQDPELWGYDAVEFKPERFAEGISKAAKNQVSYFPFALGPRICVGQNFALVEAKMAMAMILQNYSF---------ELSPSYVHAPTAVPT 840
            +FAG ET S  L +  +LL KH D + +  +E+ +V G N+ P Y+    +   + +++E+ R    + + L+R V K+TK  +  LP G ++   +  V +DP+ +  +  +F P+ F +   +  KN  ++ PF++G R C G+  A +E  + +  I+QN+ F         ++SP  V   T  PT
Sbjct:  299 FFAGTETVSTTLRYGFLLLMKHPDIEAKVHEEIDRVIGRNRQPKYEDRMKMPYTEAVIHEIQRFADMIPMGLARRVTKDTKFRDFLLPKGTEVFPMLGSVLKDPKFFS-NPKDFNPKHFLDDKGQFKKND-AFVPFSIGKRYCFGEGLARMELFLFLTNIMQNFHFKSTQAPQDIDVSPRLVGFATIPPT 486          
BLAST of CF832451 vs. ExPASy Swiss-Prot
Match: C71D8_SOYBN (Cytochrome P450 71D8 OS=Glycine max GN=CYP71D8 PE=2 SV=1)

HSP 1 Score: 90.1225 bits (222), Expect = 1.722e-17
Identity = 48/167 (28.74%), Postives = 85/167 (50.90%), Query Frame = -3
Query:  343 YFAGQETTSVLLNWTMVLLSKHQDWQERARQEVLQVF-GNNKPNYDGLNHLKIVQMILYEVLRLYPPVTVLSRSVLKETKLGNLTLPAGVQIGLPMILVHQDPELWGYDAVEFKPERFAEGISKAAKNQVSYFPFALGPRICVGQNFALVEAKMAMAMILQNYSFEL 840
            + AG +T++  L W M  + K+   +E+A+ E+ Q+F G        L  L  ++ ++ E LRL+PP  ++ R  +  T +    +P   ++ +    + +DP+ W  DA  F PERF +       N   Y PF  G R+C G  F L    + +A++L ++++EL
Sbjct:  304 FAAGTDTSASTLEWAMSEMMKNPKVKEKAQAELRQIFKGKEIIRETDLEELSYLKSVIKETLRLHPPSQLIPRECIISTNIDGYEIPIKTKVMINTWAIGRDPQYWS-DADRFIPERFNDSSIDFKGNSFEYIPFGAGRRMCPGMTFGLASITLPLALLLYHFNWEL 469          
BLAST of CF832451 vs. ExPASy Swiss-Prot
Match: C4AC1_DROME (Probable cytochrome P450 4ac1 OS=Drosophila melanogaster GN=Cyp4ac1 PE=2 SV=1)

HSP 1 Score: 90.1225 bits (222), Expect = 1.722e-17
Identity = 51/178 (28.65%), Postives = 102/178 (57.30%), Query Frame = -3
Query:  334 VIEECKLFYFAGQETTSVLLNWTMVLLSKHQDWQERARQEVLQVFGNNKP-NYDGLNHLKIVQMILYEVLRLYPPVTVLSRSVLKETKLGNLTLPAGVQIGLPMILVHQDPELWGYDAVEFKPERFAEGISKAAKNQVSYFPFALGPRICVGQNFALVEAKMAMAMILQNYSFELSPS 864
            + +E   F F G +TTS  L +T+++L+ H+D Q++  +EV  +  ++   +    N L  ++ ++ E LR++P V  + R  ++ET +  + +P   QI + +  + +DP  +    + F+P+RF    +   ++  +Y PF+ G R C+GQ FA++E K+ +A +++N  F+L P+
Sbjct:  307 ICDEVNTFMFEGYDTTSTCLIFTLLMLALHEDVQKKCYEEVENLPEDSDDISMFQFNKLVYLECVIKESLRMFPSVPFIGRQCVEETVVNGMVMPKDTQISIHIYDIMRDPRHFPKPDL-FQPDRFLPE-NTVNRHPFAYVPFSAGQRNCIGQKFAILEMKVLLAAVIRN--FKLLPA 480          
BLAST of CF832451 vs. ExPASy Swiss-Prot
Match: CP6A9_DROME (Cytochrome P450 6a9 OS=Drosophila melanogaster GN=Cyp6a9 PE=1 SV=3)

HSP 1 Score: 89.7373 bits (221), Expect = 2.249e-17
Identity = 49/177 (27.68%), Postives = 96/177 (54.24%), Query Frame = -3
Query:  343 LFYFAGQETTSVLLNWTMVLLSKHQDWQERARQEVLQVFG--NNKPNYDGLNHLKIVQMILYEVLRLYPPVTVLSRSVLKETKLGN---LTLPAGVQIGLPMILVHQDPELWG----YDAVEFKPERFAEGISKAAKNQVSYFPFALGPRICVGQNFALVEAKMAMAMILQNYSFEL 846
            +F+ AG ET+S  + + +  L++HQD Q+R R+E  +V G  N +  Y+ +  +  +  ++ E LRLY  + VL+R  L++ ++       +  G+ + +P   +H+D +L+     ++   F PER  E      ++ V + PF  GPR C+G  F  ++A+  +A+++  + F +
Sbjct:  304 VFFGAGFETSSTTMGFALYELAQHQDIQDRVRKECQEVIGKYNGEITYESMKDMVYLDQVISETLRLYTVLPVLNRECLEDYEVPGHPKYVIKKGMPVLIPCGAMHRDEKLYANPNTFNPDNFSPERVKE------RDSVEWLPFGDGPRNCIGMRFGQMQARSGLALLINRFKFSV 474          
BLAST of CF832451 vs. ExPASy Swiss-Prot
Match: CP391_DROME (Probable cytochrome P450 309a1 OS=Drosophila melanogaster GN=Cyp309a1 PE=1 SV=3)

HSP 1 Score: 89.7373 bits (221), Expect = 2.249e-17
Identity = 53/168 (31.55%), Postives = 84/168 (50.00%), Query Frame = -3
Query:  346 GQETTSVLLNWTMVLLSKHQDWQERARQEVLQVFGNN-KPNYDGLNHLKIVQMILYEVLRLYPPVTVLSRSVLKETKLG-----NLTLPAGVQIGLPMILVHQDPELWGYDAVEFKPERFAEGISKAAKNQVSYFPFALGPRICVGQNFALVEAKMAMAMILQNYSFE 831
            G ET+  +L   +  LS+H + QE+ R E+L+   +  + +YD +N+L  +     E LRL  P+    R   K T++       L L  GV + +P    H D +++  +A EF+P+RF  G +     +  + PF  GPRIC+G     +  K A+  IL NY  E
Sbjct:  333 GFETSGAVLYHMLYSLSEHHEEQEKLRSEILEALASEGQISYDQINNLPYLDQCFNESLRLTTPIGFFMRICTKPTQINLGDDKTLDLEPGVTVMVPAYQYHHDNDIYP-EASEFRPDRFENGAASVLTKRGCFLPFGDGPRICLGMRVGQLSVKTAIVHILSNYQVE 499          
BLAST of CF832451 vs. ExPASy Swiss-Prot
Match: CP120_SYNY3 (Putative cytochrome P450 120 OS=Synechocystis sp. (strain PCC 6803) GN=cyp120 PE=1 SV=1)

HSP 1 Score: 89.7373 bits (221), Expect = 2.249e-17
Identity = 57/199 (28.64%), Postives = 96/199 (48.24%), Query Frame = -3
Query:  262 EECKLFYFAGQETTSVLLNWTMVLLSKHQDWQERARQEVLQVFGNNKPNYDGLNHLKIVQMILYEVLRLYPPVTVLSRSVLKETKLGNLTLPAGVQIGLPMILVHQDPELWGYDAVEFKPERFAEGISKAAKNQVSYFPFALGPRICVGQNFALVEAKMAMAMILQNYSFELSPSYVHAPTAVPTLHPELGTQLILRKL 858
            ++  L  FAG ET +  L+   +LL +H D +ER RQE  ++  + +   + L  +  +  +L EVLRL PPV    R ++++ +      P G  +   +   H DP+L+  D  +F PERF    S       ++ PF  G R C+G+ FA +E K+    ++Q + + L P         P+  P+   ++ L  L
Sbjct:  246 DQILLLLFAGHETLTSALSSFCLLLGQHSDIRERVRQEQNKLQLSQELTAETLKKMPYLDQVLQEVLRLIPPVGGGFRELIQDCQFQGFHFPKGWLVSYQISQTHADPDLYP-DPEKFDPERFTPDGSATHNPPFAHVPFGGGLRECLGKEFARLEMKLFATRLIQQFDWTLLPGQNLELVVTPSPRPKDNLRVKLHSL 443          
BLAST of CF832451 vs. ExPASy Swiss-Prot
Match: C97B1_PEA (Cytochrome P450 97B1 OS=Pisum sativum GN=CYP97B1 PE=2 SV=1)

HSP 1 Score: 89.7373 bits (221), Expect = 2.249e-17
Identity = 58/182 (31.87%), Postives = 90/182 (49.45%), Query Frame = -3
Query:  376 AGQETTSVLLNWTMVLLSKHQDWQERARQEVLQVFGNNKPNYDGLNHLKIVQMILYEVLRLYPPVTVLSRSVLKETKL--------GNLTLPAGVQIGLPMILVHQDPELWGYDAVEFKPERF--------AEG-------------ISKAAKNQVSYFPFALGPRICVGQNFALVEAKMAM 834
            AG ETT+ +L W + LL+++ D  ++A+ EV  V G  KP ++ L  L+ +++I+ E LRLYP   +L R  LK   L           T+PAG  + + +  +H+ P  W     +F+PERF         EG                   +  ++ PF  GPR CVG  FAL+E+ +A+
Sbjct:  362 AGHETTAAVLTWAVFLLAQNPDKMKKAQAEVDLVLGMGKPTFELLKKLEYIRLIVVETLRLYPQPPLLIRRSLKPDVLPGGHKGDKDGYTIPAGTDVFISVYNLHRSPYFWDRPN-DFEPERFLVQNNNEEVEGWAGFDPSRSPGALYPNEIISDFAFLPFGGGPRKCVGDQFALMESTVAL 542          
BLAST of CF832451 vs. ExPASy Swiss-Prot
Match: C6A18_DROME (Probable cytochrome P450 6a18 OS=Drosophila melanogaster GN=Cyp6a18 PE=2 SV=1)

HSP 1 Score: 88.9669 bits (219), Expect = 3.837e-17
Identity = 53/177 (29.94%), Postives = 99/177 (55.93%), Query Frame = -3
Query:  349 VIEECKLFYFAGQETTSVLLNWTMVLLSKHQDWQERARQEVLQVFGNN--KPNYDGLNHLKIVQMILYEVLRLYPPVTVLSRSVLKETKL-GN--LTLPAGVQIGLPMILVHQDPELWGYDAVEFKPERFAEGISKAAKNQVSYFPFALGPRICVGQNFALVEAKMAMAMILQNYSF 864
            ++ +  +F+ AG +T+S  + + +  L+K+   Q++ R E+ QV   +  K  Y+ +  LK +  ++ E LR Y  V  + R   K   + GN    + AG  + +P   +H DP ++  +  EF+PERF+   S A +  V++ PF  GPR C+G  F  ++A++ +AM+++N++F
Sbjct:  300 IVAQAFVFFIAGFDTSSSTMGFALYELAKNPSIQDKVRAELGQVLEQHDQKFTYECIKDLKYLDQVINETLRHYTIVPNVDRVAAKRFVVPGNPKFVIEAGQSVIIPSSAIHHDPSIYP-EPNEFRPERFSPEES-AKRPSVAWLPFGEGPRNCIGLRFGQMQARIGLAMLIKNFTF 474          
BLAST of CF832451 vs. ExPASy Swiss-Prot
Match: THAS_PIG (Thromboxane-A synthase OS=Sus scrofa GN=TBXAS1 PE=2 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 5.011e-17
Identity = 54/179 (30.17%), Postives = 91/179 (50.84%), Query Frame = -3
Query:  337 VIEECKLFYFAGQETTSVLLNWTMVLLSKHQDWQERARQEVLQVFGNNKPNYDGLN---HLKIVQMILYEVLRLYPPVTVLSRSVLKETKLGNLTLPAGVQIGLPMILVHQDPELWGYDAVEFKPERFAEGISKAAKNQVSYFPFALGPRICVGQNFALVEAKMAMAMILQNYSFELSP 864
            V+ +  LF  AG E  +  L++   LL+ + D QE+  +EV   F    P+ +  +    L  + M+L E LR+YPP    +R   ++ ++    +PAG  + + +  +H DP+ W +    F PERF    ++  +   +Y PF  GPR C+G    L+E K+ +  IL+ + FE  P
Sbjct:  332 VVGQAFLFLIAGYEIITNTLSFVTYLLATNPDCQEKLLREV-DDFSKKHPSPEHCSLQQGLPYLDMVLSETLRMYPPAFRFTREAARDCEVLGQRIPAGTVLEVAVGALHHDPKHWPHPET-FDPERFT-AEAQRLQQPFTYLPFGAGPRSCLGVQLGLLEIKLTLLHILRKFRFEACP 507          
The following BLAST results are available for this feature:
BLAST of CF832451 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 457
Match NameE-valueIdentityDescription
C99A2_ORYSJ1.319e-1730.77Cytochrome P450 99A2 OS=Oryza sativa subsp. japoni... [more]
CP2A5_MOUSE1.722e-1728.95Cytochrome P450 2A5 OS=Mus musculus GN=Cyp2a5 PE=2... [more]
C71D8_SOYBN1.722e-1728.74Cytochrome P450 71D8 OS=Glycine max GN=CYP71D8 PE=... [more]
C4AC1_DROME1.722e-1728.65Probable cytochrome P450 4ac1 OS=Drosophila melano... [more]
CP6A9_DROME2.249e-1727.68Cytochrome P450 6a9 OS=Drosophila melanogaster GN=... [more]
CP391_DROME2.249e-1731.55Probable cytochrome P450 309a1 OS=Drosophila melan... [more]
CP120_SYNY32.249e-1728.64Putative cytochrome P450 120 OS=Synechocystis sp. ... [more]
C97B1_PEA2.249e-1731.87Cytochrome P450 97B1 OS=Pisum sativum GN=CYP97B1 P... [more]
C6A18_DROME3.837e-1729.94Probable cytochrome P450 6a18 OS=Drosophila melano... [more]
THAS_PIG5.011e-1730.17Thromboxane-A synthase OS=Sus scrofa GN=TBXAS1 PE=... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS02_01O03_f Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa01O03, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF832451 ID=CF832451; Name=CF832451; organism=Citrus sinensis; type=EST; length=866bp
TTTTTTTTTTTTTTACCCAGCATTAATGCTCTAATTCTAATCATGGTATT
ATAAAATTTTCCAATGTGGGGAAACATCTTTACTCTAAATAAAACTAGAG
TTGTGTTGAGCTCAATTTAAAAATCTAAACAGCTCTTGTCTAAGCTTTGA
ATCTCAATACAAAATTAAATACATTAAACTTCATTATAAAAGAGTCTACA
TTGGCATCATGCTAATCTTATTGACAATATATAGTTGCCGGTATTAATTA
TTTTTGCACCTTAGTTTCCGTAGAATCAATTGGGTACCCAGTTCCGGATG
AAGGGTTGGAACCGCCGTTGGAGCATGGACATAGGATGGAGACAACTCGA
ATGAGTAGTTTTGAAGAATCATTGCCATGGCCATTTTTGCTTCTACTAGA
GCAAAATTTTGTCCAACACATATCCTAGGACCCAATGCGAATGGGAAATA
TGAAACTTGATTTTTGGCCGCCTTTGAAATTCCTTCCGCAAACCTTTCCG
GCTTAAACTCAACTGCATCGTAACCCCAAAGTTCAGGATCCTGGTGAACT
AGGATCATTGGCAAGCCAATTTGAACCCCAGCGGGTAATGTCAAGTTTCC
GAGTTTCGTTTCCTTAAGAACAGATCGACTAAGCACAGTTACAGGCGGAT
AAAGCCTGAGTACCTCGTAAAGTATCATCTGGACAATTTTGAGGTGATTC
AATCCATCATAGTTGGGCTTATTATTGCCAAACACTTGCAAAACCTCTTG
CCTTGCACGTTCTTGCCAATCTTGATGCTTGCTCAATAAGACCATCGTCC
AATTAAGCAAAACAGAGGTAGTCTCTTGGCCGGCAAAGTAAAATAGCTTA
CACTCTTCGATCACAT
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