CF832451

Overview
NameCF832451
Unique NameCF832451
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length866
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CF832451 vs. ExPASy Swiss-Prot
Match: CP2A4_MOUSE (Cytochrome P450 2A4 OS=Mus musculus GN=Cyp2a4 PE=2 SV=2)

HSP 1 Score: 85.1149 bits (209), Expect = 5.540e-16
Identity = 53/190 (27.89%), Postives = 99/190 (52.11%), Query Frame = -3
Query:  304 YFAGQETTSVLLNWTMVLLSKHQDWQERARQEVLQVFGNNK-PNYDGLNHLKIVQMILYEVLRLYPPVTV-LSRSVLKETKLGNLTLPAGVQIGLPMILVHQDPELWGYDAVEFKPERFAEGISKAAKNQVSYFPFALGPRICVGQNFALVEAKMAMAMILQNYSF---------ELSPSYVHAPTAVPT 840
            +FAG ET S  L +  +LL K+ D + +  +E+ +V G N+ P Y+    +   + +++E+ R    + + L+R V K+TK  +  LP G ++   +  V +DP+ +  +  +F P+ F +   +  K+  ++ PF++G R C G+  A +E  + +  I+QN+ F         ++SP  V   T  PT
Sbjct:  299 FFAGTETVSTTLRYGFLLLMKYPDIEAKVHEEIDRVIGRNRQPKYEDRMKMPYTEAVIHEIQRFADLIPMGLARRVTKDTKFRDFLLPKGTEVFPMLGSVLKDPKFFS-NPKDFNPKHFLDDKGQFKKSD-AFVPFSIGKRYCFGEGLARMELFLFLTNIMQNFHFKSTQAPQDIDVSPRLVGFVTIPPT 486          
BLAST of CF832451 vs. ExPASy Swiss-Prot
Match: CP17A_FELCA (Steroid 17-alpha-hydroxylase/17,20 lyase OS=Felis catus GN=CYP17A1 PE=2 SV=1)

HSP 1 Score: 85.1149 bits (209), Expect = 5.540e-16
Identity = 48/167 (28.74%), Postives = 88/167 (52.69%), Query Frame = -3
Query:  343 AGQETTSVLLNWTMVLLSKHQDWQERARQEVLQVFGNNK-PNYDGLNHLKIVQMILYEVLRLYPPV-TVLSRSVLKETKLGNLTLPAGVQIGLPMILVHQDPELWGYDAVEFKPERFAEGI-SKAAKNQVSYFPFALGPRICVGQNFALVEAKMAMAMILQNYSFEL 834
            AG ETT+ ++ WT+  L  H    ++ ++E+ Q  G ++ P     N L +++  + EVLR+ P   T++    + ++ +G   +  G  + + +  +H + + W Y   +F PERF +   S+     +SY PF  GPR C+G++ A  E  + MA +LQ +  E+
Sbjct:  302 AGVETTTSVVRWTVAFLLHHPQLYKKLQEEIDQNIGFSRTPTMSDRNQLILLEATIREVLRIRPVAPTLIPHKAIMDSSIGEFAVDKGTNVIINLWALHHNEKEW-YRPDQFMPERFLDPTRSQLISPSLSYLPFGAGPRSCLGESLARQEVFLFMAWLLQRFDLEV 467          
BLAST of CF832451 vs. ExPASy Swiss-Prot
Match: CP2J6_MOUSE (Cytochrome P450 2J6 OS=Mus musculus GN=Cyp2j6 PE=2 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 7.236e-16
Identity = 52/189 (27.51%), Postives = 93/189 (49.21%), Query Frame = -3
Query:  298 YFAGQETTSVLLNWTMVLLSKHQDWQERARQEVLQVFGNNK-PNYDGLNHLKIVQMILYEVLRLYPPVTV-LSRSVLKETKLGNLTLPAGVQIGLPMILVHQDPELWGYDAVEFKPERFAEGISKAAKNQVSYFPFALGPRICVGQNFALVEAKMAMAMILQNYSF------ELSPSYVHAPTAVPTLH 840
            +FAG ETTS  L W ++ ++ + + QE+ + E+ +V G  +         +     +++EV R+   + + + R V  +T L    LP G  +   +  +H+DP+ W    V F PE F E  +   K + S+ PF++G R C+G+  A  E  +    ++Q ++F      +LSP + +  T  P  H
Sbjct:  308 FFAGTETTSTTLRWALLYMALYPEVQEKVQAEIDRVIGQKRAARLADRESMPYTNAVIHEVRRMGNIIPLNVPREVAMDTNLNGFHLPKGTMVLTNLTALHRDPKEWATPDV-FNPEHFLE--NGQFKKRESFLPFSMGKRACLGEQLARSELFIFFTSLMQKFTFNPPINEKLSPKFRNGLTLSPVSH 493          
BLAST of CF832451 vs. ExPASy Swiss-Prot
Match: C4AD1_DROME (Probable cytochrome P450 4ad1 OS=Drosophila melanogaster GN=Cyp4ad1 PE=2 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 7.236e-16
Identity = 48/188 (25.53%), Postives = 95/188 (50.53%), Query Frame = -3
Query:  310 VIEECKLFYFAGQETTSVLLNWTMVLLSKHQDWQERARQEVLQVFGNN---KPNYDGLNHLKIVQMILYEVLRLYPPVTVLSRSVLKETKLGNLTLPAGVQIGLPMILVHQDPELWGYDAVEFKPERFAEGISKAAKNQVSYFPFALGPRICVGQNFALVEAKMAMAMILQNYSFELSPSYVHAPTAV 864
            +IEE   F F G +  +  +++T+  LS+H + Q++A +E  ++FG N   + +   L+ +  +++I+ E LRLYP V +++R+      +    +     + + +I +  + E +  D   F+PERF               PF+ GPR C+ + FA+ + K  ++ +L+   FE+ P+    P  +
Sbjct:  295 IIEEVSTFIFTGHDPIAAAISFTLYTLSRHSEIQQKAAEEQRRIFGENFAGEADLARLDQMHYLELIIRETLRLYPSVPLIARTNRNPIDINGTKVAKCTTVIMCLIAMGYN-EKYFDDPCTFRPERFENPTGNVGIEAFKSVPFSAGPRRCIAEKFAMYQMKALLSQLLRR--FEILPAVDGLPPGI 479          
BLAST of CF832451 vs. ExPASy Swiss-Prot
Match: CP17A_MOUSE (Steroid 17-alpha-hydroxylase/17,20 lyase OS=Mus musculus GN=Cyp17a1 PE=2 SV=1)

HSP 1 Score: 84.3445 bits (207), Expect = 9.450e-16
Identity = 55/188 (29.26%), Postives = 93/188 (49.47%), Query Frame = -3
Query:  295 AGQETTSVLLNWTMVLLSKHQDWQERARQEVLQVFGNNK-PNYDGLNHLKIVQMILYEVLRLYPPVTVL-SRSVLKETKLGNLTLPAGVQIGLPMILVHQDPELWGYDAVEFKPERFAEGI-SKAAKNQVSYFPFALGPRICVGQNFALVEAKMAMAMILQNYSFELS-----PSYVHAPTAVPTLHP 834
            AG ETTS +LNW +  L  + + + + ++E+ Q  G ++ P+++   HL +++  + EVLR+ P   +L       ++ +G   +P    + + +  +H D   W      F PERF +   S       SY PF  GPR C+G+  A  E  + MA++LQ + F++S     P  V  P  V  + P
Sbjct:  301 AGIETTSSVLNWILAFLVHNPEVKRKIQKEIDQYVGFSRTPSFNDRTHLLMLEATIREVLRIRPVAPLLIPHKANIDSSIGEFAIPKDTHVIINLWALHHDKNEWDQPD-RFMPERFLDPTGSHLITPTPSYLPFGAGPRSCIGEALARQELFIFMALLLQRFDFDVSDDKQLPCLVGDPKVVFLIDP 487          
BLAST of CF832451 vs. ExPASy Swiss-Prot
Match: CP52H_CANTR (Cytochrome P450 52A8 OS=Candida tropicalis GN=CYP52A8 PE=2 SV=1)

HSP 1 Score: 83.9593 bits (206), Expect = 1.234e-15
Identity = 62/205 (30.24%), Postives = 102/205 (49.76%), Query Frame = -3
Query:  268 AGQETTSVLLNWTMVLLSKHQDWQERARQEVLQVFGNNKPN------YDGLNHLKIVQMILYEVLRLYPPVTVLSRSVLKETKL-------GN--LTLPAGVQIGLPMILVHQDPELWGYDAVEFKPERFAEGISKAAKNQVSYFPFALGPRICVGQNFALVEAKMAMAMILQNY-SFELSPSYVHAPTAVPTLHPELGTQLILR 834
            AG++T++ LL++ +  L+++     + R++V   FG  + +      ++ L     ++  L E LR+YP V    R   K T L       GN  + +  G  +   +   H DP  +G DA EF+PER+ E  ++  K   +Y PF  GPRIC+GQ FAL EA   +  + Q++ + EL P  V+ P  +  L   L    I++
Sbjct:  313 AGRDTSAGLLSFAVFELARNPHIWAKLREDVESQFGLGEQSRIEEITFESLKRCGYLKAFLNETLRVYPSVPRNFRIATKNTTLPRGGGSDGNSPVLVKKGEAVSYGINSTHLDPVYYGDDAAEFRPERWNEPSTR--KLGWAYLPFNGGPRICLGQQFALTEAGYVLVRLAQSFDTLELKPPVVYPPKRLTNLTMSLQDGTIVK 515          
BLAST of CF832451 vs. ExPASy Swiss-Prot
Match: CP52E_CANMA (Cytochrome P450 52A5 OS=Candida maltosa GN=CYP52A5 PE=1 SV=1)

HSP 1 Score: 83.9593 bits (206), Expect = 1.234e-15
Identity = 56/187 (29.95%), Postives = 95/187 (50.80%), Query Frame = -3
Query:  319 AGQETTSVLLNWTMVLLSKHQDWQERARQEVLQVFGNNKP------NYDGLNHLKIVQMILYEVLRLYPPVTVLSRSVLKETKLGN---------LTLPAGVQIGLPMILVHQDPELWGYDAVEFKPERFAEGISKAAKNQVSYFPFALGPRICVGQNFALVEAKMAMAMILQNYSFELSPSYVHAP 834
            AG++TT+ LL++ M  L+++     + R+E+   FG  +       +++ L   + ++ +L E LR+YP V V  R+  ++T L           + +P G  +   +   H+  E +G DA EF+PER+ E  ++  K   +Y PF  GPRIC+GQ FAL EA   +  + Q +    S    + P
Sbjct:  320 AGRDTTAGLLSFAMFELARNPKIWNKLREEIEVNFGLGEEARVDEISFETLKKCEYLKAVLNETLRMYPSVPVNFRTATRDTTLPRGGGKDGTSPIFVPKGSSVVYTVYKTHRLEEYYGKDAYEFRPERWFEPSTR--KLGWAYVPFNGGPRICLGQQFALTEASYVITRLAQMFEHLESKDETYPP 504          
BLAST of CF832451 vs. ExPASy Swiss-Prot
Match: CP51A_PIG (Lanosterol 14-alpha demethylase OS=Sus scrofa GN=CYP51A1 PE=2 SV=1)

HSP 1 Score: 83.9593 bits (206), Expect = 1.234e-15
Identity = 50/181 (27.62%), Postives = 86/181 (47.51%), Query Frame = -3
Query:  298 AGQETTSVLLNWTMVLLSKHQDWQERARQEVLQVFGNNKP--NYDGLNHLKIVQMILYEVLRLYPPVTVLSRSVLKETKLGNLTLPAGVQIGLPMILVHQDPELWGYDAVEFKPERFAEGISKAAKNQVSYFPFALGPRICVGQNFALVEAKMAMAMILQNYSFELSPSYVHAPTAVPTLH 834
            AGQ T+S    W    L++ +  QE+   E   V G + P   YD L  L ++   + E LRL PP+  + R       +   T+P G Q+ +   +  +  + W  + ++F P+R+ +  + A+  + +Y PF  G   C+G+NFA V+ K   + +L+ Y F+L   Y         +H
Sbjct:  311 AGQHTSSTTSAWMGFFLARDKTLQEKCYLEQKTVCGEDLPPLTYDQLKDLNLLDRCIKETLRLRPPIMTMMRMAKTPQTVAGYTIPPGHQVCVSPTVNQRLKDSW-VERLDFNPDRYLQD-NPASGEKFAYVPFGAGRHRCIGENFAYVQIKTIWSTMLRLYEFDLIDGYFPTVNYTTMIH 489          
BLAST of CF832451 vs. ExPASy Swiss-Prot
Match: CP313_DROME (Probable cytochrome P450 313b1 OS=Drosophila melanogaster GN=Cyp313b1 PE=2 SV=2)

HSP 1 Score: 83.9593 bits (206), Expect = 1.234e-15
Identity = 49/175 (28.00%), Postives = 95/175 (54.29%), Query Frame = -3
Query:  355 VIEECKLFYFAGQETTSVLLNWTMVLLSKHQDWQERARQE-VLQVFGNNKPNYDGLNHLKIVQMILYEVLRLYPPVTVLSRSVLKETKL----GNLTLPAGVQIGLPMILVHQDPELWGYDAVEFKPERFAEGISKAAKNQVSYFPFALGPRICVGQNFALVEAKMAMAMILQNY 864
            V +E  +   A  ETTS  L +T++ L+ H  +QE+  +E V ++  +   N + L  L+  +M++ E +RL+ PV ++ RS  ++ +L    G   +P G QIG+ +  + +D  +WG  +  + P+    G+    ++  ++ PF  G R+C+G  +A +  K+ +A I ++Y
Sbjct:  297 VRDEANVTIAATFETTSTALYFTILCLAMHPCYQEKLHKELVTELPPSGDINLEQLQRLEYTEMVINEAMRLFAPVPMVLRSADQDIQLKRGDGEFLIPRGTQIGIDIYNMQRDERVWGPLSRTYNPDAHF-GLDSPQRHAFAFVPFTKGLRMCIGYRYAQMLMKLLLARIFRSY 470          
BLAST of CF832451 vs. ExPASy Swiss-Prot
Match: CP2J3_RAT (Cytochrome P450 2J3 OS=Rattus norvegicus GN=Cyp2j3 PE=2 SV=1)

HSP 1 Score: 83.9593 bits (206), Expect = 1.234e-15
Identity = 48/167 (28.74%), Postives = 87/167 (52.10%), Query Frame = -3
Query:  346 YFAGQETTSVLLNWTMVLLSKHQDWQERARQEVLQVFGNNK-PNYDGLNHLKIVQMILYEVLRLYPPVTV-LSRSVLKETKLGNLTLPAGVQIGLPMILVHQDPELWGYDAVEFKPERFAEGISKAAKNQVSYFPFALGPRICVGQNFALVEAKMAMAMILQNYSFE 840
            +FAG ETTS  L W ++ ++ + + QE+ + E+ +V G  + PN    + +     +++EV R+   +   + R V  +T L    LP G  I   +  +H+DP+ W      F PE F E  +   K + S+ PF++G R C+G+  A  E  + +  ++Q ++F+
Sbjct:  309 FFAGTETTSTTLRWALLCMALYPEVQEKMQAEIDRVIGQGRQPNLADRDSMPYTNAVIHEVQRIGNIIPFNVPREVAVDTYLAGFNLPKGTMILTNLTALHRDPKEWATPDT-FNPEHFLE--NGQFKKRESFLPFSMGKRACLGEQLARSELFIFITSLIQKFTFK 472          
The following BLAST results are available for this feature:
BLAST of CF832451 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 457
Match NameE-valueIdentityDescription
CP2A4_MOUSE5.540e-1627.89Cytochrome P450 2A4 OS=Mus musculus GN=Cyp2a4 PE=2... [more]
CP17A_FELCA5.540e-1628.74Steroid 17-alpha-hydroxylase/17,20 lyase OS=Felis ... [more]
CP2J6_MOUSE7.236e-1627.51Cytochrome P450 2J6 OS=Mus musculus GN=Cyp2j6 PE=2... [more]
C4AD1_DROME7.236e-1625.53Probable cytochrome P450 4ad1 OS=Drosophila melano... [more]
CP17A_MOUSE9.450e-1629.26Steroid 17-alpha-hydroxylase/17,20 lyase OS=Mus mu... [more]
CP52H_CANTR1.234e-1530.24Cytochrome P450 52A8 OS=Candida tropicalis GN=CYP5... [more]
CP52E_CANMA1.234e-1529.95Cytochrome P450 52A5 OS=Candida maltosa GN=CYP52A5... [more]
CP51A_PIG1.234e-1527.62Lanosterol 14-alpha demethylase OS=Sus scrofa GN=C... [more]
CP313_DROME1.234e-1528.00Probable cytochrome P450 313b1 OS=Drosophila melan... [more]
CP2J3_RAT1.234e-1528.74Cytochrome P450 2J3 OS=Rattus norvegicus GN=Cyp2j3... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS02_01O03_f Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa01O03, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF832451 ID=CF832451; Name=CF832451; organism=Citrus sinensis; type=EST; length=866bp
TTTTTTTTTTTTTTACCCAGCATTAATGCTCTAATTCTAATCATGGTATT
ATAAAATTTTCCAATGTGGGGAAACATCTTTACTCTAAATAAAACTAGAG
TTGTGTTGAGCTCAATTTAAAAATCTAAACAGCTCTTGTCTAAGCTTTGA
ATCTCAATACAAAATTAAATACATTAAACTTCATTATAAAAGAGTCTACA
TTGGCATCATGCTAATCTTATTGACAATATATAGTTGCCGGTATTAATTA
TTTTTGCACCTTAGTTTCCGTAGAATCAATTGGGTACCCAGTTCCGGATG
AAGGGTTGGAACCGCCGTTGGAGCATGGACATAGGATGGAGACAACTCGA
ATGAGTAGTTTTGAAGAATCATTGCCATGGCCATTTTTGCTTCTACTAGA
GCAAAATTTTGTCCAACACATATCCTAGGACCCAATGCGAATGGGAAATA
TGAAACTTGATTTTTGGCCGCCTTTGAAATTCCTTCCGCAAACCTTTCCG
GCTTAAACTCAACTGCATCGTAACCCCAAAGTTCAGGATCCTGGTGAACT
AGGATCATTGGCAAGCCAATTTGAACCCCAGCGGGTAATGTCAAGTTTCC
GAGTTTCGTTTCCTTAAGAACAGATCGACTAAGCACAGTTACAGGCGGAT
AAAGCCTGAGTACCTCGTAAAGTATCATCTGGACAATTTTGAGGTGATTC
AATCCATCATAGTTGGGCTTATTATTGCCAAACACTTGCAAAACCTCTTG
CCTTGCACGTTCTTGCCAATCTTGATGCTTGCTCAATAAGACCATCGTCC
AATTAAGCAAAACAGAGGTAGTCTCTTGGCCGGCAAAGTAAAATAGCTTA
CACTCTTCGATCACAT
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