CF832451

Overview
NameCF832451
Unique NameCF832451
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length866
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CF832451 vs. ExPASy Swiss-Prot
Match: CP6D3_MUSDO (Cytochrome P450 6d3 OS=Musca domestica GN=CYP6D3 PE=2 SV=1)

HSP 1 Score: 82.0333 bits (201), Expect = 4.690e-15
Identity = 54/194 (27.84%), Postives = 101/194 (52.06%), Query Frame = -3
Query:  307 LFYFAGQETTSVLLNWTMVLLSKHQDWQERARQEVLQVFGNN--KPN----YDGLNHLKIVQMILYEVLRLYPPVTVLSRSVLKETKLGN--LTLPAGVQIGLPMILVHQDPELWGYDAVEFKPERFAEGISKAAKNQVSYFPFALGPRICVGQNFALVEAKMAMAMILQNYSFELSP------SYVHAPTAVP 846
            LFY AG ETT+   ++T+  L+ + +  ++A+ EV +    +  KP+    Y+ +  +K + + + E  R YP +  L+R   ++ K+ N   T+     + + ++ +H+D + +  + + +KPERFA+       +  +Y PF  GPR C+ Q   ++  K+A+A IL N++ E  P       +  AP  VP
Sbjct:  314 LFYIAGSETTASSTSFTIYELAMNPEALKKAQNEVDECLKKHGIKPDGRITYEAIQDMKYLDLCVKETTRKYPGLPFLNRQCTQDFKVPNSKFTIKKDTNVIISLLGLHRDAKYFP-EPLAYKPERFADETKDY--DAAAYMPFGEGPRHCIAQRMGVMNTKVALAKILANFNIEPMPHKEAEFQFNTAPVLVP 504          
BLAST of CF832451 vs. ExPASy Swiss-Prot
Match: CP317_DROME (Probable cytochrome P450 317a1 OS=Drosophila melanogaster GN=Cyp317a1 PE=3 SV=2)

HSP 1 Score: 82.0333 bits (201), Expect = 4.690e-15
Identity = 55/200 (27.50%), Postives = 104/200 (52.00%), Query Frame = -3
Query:  262 LFYFAGQETTSVLLNWTMVLLSKHQDWQERARQEVLQVFGNNKPN--YDGLNHLKIVQMILYEVLRLYPPVTVLSRSVLKETKLGN---LTLPAGVQIGLPMILVHQDPELWGYDAVEFKPERFAEGISKAAKNQVSYFPFALGPRICVGQNFALVEAKMAMAMILQNYSFELSPSYVHAPTAVPTLHPELGTQLILRKL 846
            +F  AG E T+  L++ +  L+ H + Q R R+EV +    +  +  ++G+  L  +  ++ E LR++P    + R  L +  + +     L   + + +P   +H DP+++  D  EFKP+R++ G   + + Q ++F F +G R C+G  FA ++ ++A+A++L  Y F L+       T  P ++ E G  L L  L
Sbjct:  316 VFLKAGYEQTANTLSYVLYELALHPELQVRVREEVKKAIERHDGHITHEGIKSLSFMGQVINETLRMHPITPYILRRTLNDYAVPDHPKYILVKELFLIIPTHAIHHDPDIYP-DPEEFKPDRWS-GPRDSLQEQGTWFGFGVGARSCIGIQFAQLQLRLALALLLSEYEFSLN-------TRKPLINLEDGIALTLMPL 506          
BLAST of CF832451 vs. ExPASy Swiss-Prot
Match: CP27A_RAT (Sterol 26-hydroxylase, mitochondrial OS=Rattus norvegicus GN=Cyp27a1 PE=1 SV=1)

HSP 1 Score: 82.0333 bits (201), Expect = 4.690e-15
Identity = 55/172 (31.98%), Postives = 88/172 (51.16%), Query Frame = -3
Query:  337 AGQETTSVLLNWTMVLLSKHQDWQERARQEVLQV--FGNNKPNYDGLNHLKIVQMILYEVLRLYPPVTVLSRSVL-KETKLGNLTLPAGVQIGLPMILVHQDPELWGYDAVEFKPERFA---EGISKAAKNQVSYFPFALGPRICVGQNFALVEAKMAMAMILQNYSFELSP 834
            AG +TTS  L W +  LSK+ + QE   +EV  V  FG    N D   H+ +++ ++ E LRLYP V   SR +  KET++     P   Q  L   +V +DP ++  +   F+P R+    E  +   ++     PF  G R C+G+  A +E ++ ++ ++Q Y   LSP
Sbjct:  337 AGVDTTSNTLTWALYHLSKNPEIQEALHKEVTGVVPFGKVPQNKD-FAHMPLLKAVIKETLRLYPVVPTNSRIITEKETEINGFLFPKNTQFVLCHYVVSRDPSVFP-EPESFQPHRWLRKREDDNSGIQHPFGSVPFGYGVRSCLGRRIAELEMQLLLSRLIQKYEVVLSP 506          
BLAST of CF832451 vs. ExPASy Swiss-Prot
Match: C99A3_ORYSJ (Cytochrome P450 99A3 OS=Oryza sativa subsp. japonica GN=CYP99A3 PE=2 SV=1)

HSP 1 Score: 82.0333 bits (201), Expect = 4.690e-15
Identity = 50/169 (29.59%), Postives = 89/169 (52.66%), Query Frame = -3
Query:  343 YFAGQETTSVLLNWTMVLLSKHQDWQERARQEVLQVFGNNKP-NYDGL-NHLKIVQMILYEVLRLYPPVTVLSRSVLKET-KLGNLTLPAGVQIGLPMILVHQDPELWGYDAVEFKPERFAEGISKAAKNQVSYFPFALGPRICVGQNFALVEAKMAMAMILQNYSFEL 840
            +  G ETTS    W M  L ++ +   + + EV  VF N  P +++GL  +L  +++++ E LRL P + +L   + +ET ++G   +  G ++ +    + + PE W  DA +F PERF +G +    ++  Y PF  G R C G  FA+   ++ +A +L  + + L
Sbjct:  296 FTGGTETTSSTTEWLMSELMRNPEVMAKVQAEVRGVFDNKSPQDHEGLLENLSYMKLVIKETLRLNPVLPLLLPHLCRETCEIGGYEIVEGTRVLINSWAMARSPEYWD-DAEKFIPERFEDGTADFKGSRFEYLPFGTGRRRCPGDIFAMATLELIVARLLYYFDWSL 463          
BLAST of CF832451 vs. ExPASy Swiss-Prot
Match: CP51_DICDI (Probable lanosterol 14-alpha demethylase OS=Dictyostelium discoideum GN=cyp51 PE=3 SV=1)

HSP 1 Score: 81.6481 bits (200), Expect = 6.125e-15
Identity = 48/176 (27.27%), Postives = 91/176 (51.70%), Query Frame = -3
Query:  337 FAGQETTSVLLNWTMVLLSKHQDWQERARQEV---LQVFGNNKPNYDGLNHLKIVQMILYEVLRLYPPVTVLSRSVLKETKLGNLTLPAG------VQIGLPMILVHQDPELWGYDAVEFKPERFAEGISKAAKNQVSYFPFALGPRICVGQNFALVEAKMAMAMILQNYSFELSP 837
            FAGQ T+S+ L +T+  L  + ++ +  ++++   +Q     + N+DGL  +  ++ ++ EVLRL+PP+  L R V+   +    T+PAG       Q+G+ +  V+++P+        F+P+RF        K   S+  F  G   C G+NF +++ K    ++   Y+ E+ P
Sbjct:  276 FAGQHTSSITLTYTIFYLLNNLEYFDETQKDINDIVQKENQGEINFDGLKRMNRLETVIREVLRLHPPLIFLMRKVMTPMEYKGKTIPAGHILAVSPQVGMRLPTVYKNPD-------SFEPKRF----DVEDKTPFSFIAFGGGKHGCPGENFGILQIKTIWTVLSTKYNLEVGP 440          
BLAST of CF832451 vs. ExPASy Swiss-Prot
Match: C71AR_ARATH (Cytochrome P450 71A27 OS=Arabidopsis thaliana GN=CYP71A27 PE=2 SV=3)

HSP 1 Score: 81.6481 bits (200), Expect = 6.125e-15
Identity = 48/173 (27.75%), Postives = 90/173 (52.02%), Query Frame = -3
Query:  343 KLFYFAGQETTSVLLNWTMVLLSKHQDWQERARQEVLQVFGNN----KPNYDGLNHLKIVQMILYEVLRLYPPVTVLSRSVLKETKLGNLTLPAGVQIGLPMILVHQDPELWGYDAVEFKPERFAEGISKAAKNQVSYFPFALGPRICVGQNFALVEAKMAMAMILQNYSFEL 849
            K  +F+G  TT+  L WTM  L +H +  ++ + E+     +N    +   + +N+L  V   + E LRL+P   +L R   ++ +L    + AG  + +    + ++P +WG DA E++PER         +    + PF  G R+C G  F+LV +K+A+A +++ +++ L
Sbjct:  294 KDMFFSGTATTASQLEWTMTELMRHPECMKKLQDEINSFSTHNLNVTEKEVEKMNYLHCV---IKEGLRLHPSGPLLFRLPSEDVQLKGYDISAGTHVIINAWALQRNPAIWGLDANEYRPERHFWYEFGFQRTDSKFVPFGAGRRLCPGIGFSLVLSKLALANLVKRFNWRL 463          
BLAST of CF832451 vs. ExPASy Swiss-Prot
Match: C71AG_ARATH (Cytochrome P450 71A16 OS=Arabidopsis thaliana GN=CYP71A16 PE=2 SV=1)

HSP 1 Score: 81.6481 bits (200), Expect = 6.125e-15
Identity = 53/184 (28.80%), Postives = 86/184 (46.74%), Query Frame = -3
Query:  310 YFAGQETTSVLLNWTMVLLSKHQDWQERARQEVLQVFGNNK----PNYDGLNHLKIVQMILYEVLRLYPPVTVLSRSVLKE-TKLGNLTLPAGVQIGLPMILVHQDPELWGYDAVEFKPERFAEGISKAAKNQVSYFPFALGPRICVGQNFALVEAKMAMAMILQNYSF--ELSPSYVHAPTAV 840
            +     TTS ++ W M  L ++ +  ++ + E+  V   N        + +N+LK V   + EVLRL+PP+ +L   +L E  KL    + AG Q+ +    + +D   WG DA EF+PER  +           Y PF  G R+C G     V A + +A +++ + +  E  PS    P  V
Sbjct:  297 FLGSTTTTSAVIEWAMTRLMRNPECLKKLQDEIRSVSKMNSYVSGKEVENMNYLKAV---IKEVLRLHPPLPLLVPRLLSEDVKLKGYDITAGTQVIINAWAIQRDTATWGSDAQEFRPERHFDSTWDFVGRNFKYIPFGAGRRLCPGIGLGSVMASVTLANLVKRFDWRVEDGPSGYDKPDLV 477          
BLAST of CF832451 vs. ExPASy Swiss-Prot
Match: CP52T_CANMA (Cytochrome P450 52D1 OS=Candida maltosa GN=CYP52D1 PE=2 SV=1)

HSP 1 Score: 81.2629 bits (199), Expect = 8.000e-15
Identity = 61/193 (31.61%), Postives = 92/193 (47.67%), Query Frame = -3
Query:  319 EECKLFYFAGQETTSVLLNWTMVLLSKHQDWQERARQEVLQVFGNNKP--NYDGLN-HLKIVQMILYEVLRLYPPVTVLSRSVLKETKLGN---------LTLPAGVQIGLPMILVHQDPELWGYDAVEFKPERFAEGISKAAKNQVSYFPFALGPRICVGQNFALVEAKMAMAMILQNYS-FELSPSYVHAP 858
            ++C     AG++TT+ LL++    L+ ++    + R EVL VF  +     +D L      +Q IL+E LRLYP V   +R   K T L +         + +  G  +   +   H D + +  DA+ F+PER+ E          SY PF  GPRIC+GQ FAL EA   +  + Q Y+   L P+    P
Sbjct:  288 DQCLNILLAGRDTTASLLSFAFFELALNEPIWIKLRTEVLHVFQTSLELITFDLLKTKCPYLQAILHETLRLYPSVPRNARFSKKNTTLPHGGGVDGMSPILIKKGQPVAYFICATHVDEKFYTKDALIFRPERWCEEPLIKKNLAWSYLPFNGGPRICLGQQFALTEASYVLTRLAQCYTKISLQPNSFEYP 480          
BLAST of CF832451 vs. ExPASy Swiss-Prot
Match: CP52A_CANTR (Cytochrome P450 52A1 OS=Candida tropicalis GN=CYP52A1 PE=1 SV=3)

HSP 1 Score: 81.2629 bits (199), Expect = 8.000e-15
Identity = 55/194 (28.35%), Postives = 97/194 (50.00%), Query Frame = -3
Query:  301 AGQETTSVLLNWTMVLLSKHQDWQERARQEVLQVFGNNKP------NYDGLNHLKIVQMILYEVLRLYPPVTVLSRSVLKETKLGN---------LTLPAGVQIGLPMILVHQDPELWGYDAVEFKPERFAEGISKAAKNQVSYFPFALGPRICVGQNFALVEAKMAMAMILQNY-SFELSPSYVHAPTAVPTL 834
            AG++TT+ LL++    LS++ +   + R+E+   FG  +       +++ L   + ++ ++ E LR+YP V    R   + T L           + +  G  +   ++  H+D +++G DA  F+PER+ E   +  K   +Y PF  GPRIC+GQ FAL EA      +LQ + + +  P+  + P    TL
Sbjct:  336 AGRDTTAGLLSFLFFELSRNPEIFAKLREEIENKFGLGQDARVEEISFETLKSCEYLKAVINETLRIYPSVPHNFRVATRNTTLPRGGGEGGLSPIAIKKGQVVMYTILATHRDKDIYGEDAYVFRPERWFE--PETRKLGWAYVPFNGGPRICLGQQFALTEASYVTVRLLQEFGNLKQDPNTEYPPKLQNTL 527          
BLAST of CF832451 vs. ExPASy Swiss-Prot
Match: CP51A_RAT (Lanosterol 14-alpha demethylase OS=Rattus norvegicus GN=Cyp51a1 PE=2 SV=1)

HSP 1 Score: 81.2629 bits (199), Expect = 8.000e-15
Identity = 48/181 (26.52%), Postives = 87/181 (48.07%), Query Frame = -3
Query:  298 AGQETTSVLLNWTMVLLSKHQDWQERARQEVLQVFGNNKP--NYDGLNHLKIVQMILYEVLRLYPPVTVLSRSVLKETKLGNLTLPAGVQIGLPMILVHQDPELWGYDAVEFKPERFAEGISKAAKNQVSYFPFALGPRICVGQNFALVEAKMAMAMILQNYSFELSPSYVHAPTAVPTLH 834
            AGQ T+S    W    L++ +  Q++   E   V G + P   Y+ L  L ++   + E LRL PP+  + R       +   T+P G Q+ +   +  +  + W  + ++F P+R+ +  + A+  + +Y PF  G   C+G+NFA V+ K   + +L+ Y F+L   Y  +      +H
Sbjct:  311 AGQHTSSTTSAWMGFFLARDKPLQDKCYLEQKTVCGEDLPPLTYEQLKDLNLLDRCIKETLRLRPPIMTMMRMAKTPQTVAGYTIPPGHQVCVSPTVNQRLKDSW-VERLDFNPDRYLQD-NPASGEKFAYVPFGAGRHRCIGENFAYVQIKTIWSTMLRLYEFDLINGYFPSVNYTTMIH 489          
The following BLAST results are available for this feature:
BLAST of CF832451 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 457
Match NameE-valueIdentityDescription
CP6D3_MUSDO4.690e-1527.84Cytochrome P450 6d3 OS=Musca domestica GN=CYP6D3 P... [more]
CP317_DROME4.690e-1527.50Probable cytochrome P450 317a1 OS=Drosophila melan... [more]
CP27A_RAT4.690e-1531.98Sterol 26-hydroxylase, mitochondrial OS=Rattus nor... [more]
C99A3_ORYSJ4.690e-1529.59Cytochrome P450 99A3 OS=Oryza sativa subsp. japoni... [more]
CP51_DICDI6.125e-1527.27Probable lanosterol 14-alpha demethylase OS=Dictyo... [more]
C71AR_ARATH6.125e-1527.75Cytochrome P450 71A27 OS=Arabidopsis thaliana GN=C... [more]
C71AG_ARATH6.125e-1528.80Cytochrome P450 71A16 OS=Arabidopsis thaliana GN=C... [more]
CP52T_CANMA8.000e-1531.61Cytochrome P450 52D1 OS=Candida maltosa GN=CYP52D1... [more]
CP52A_CANTR8.000e-1528.35Cytochrome P450 52A1 OS=Candida tropicalis GN=CYP5... [more]
CP51A_RAT8.000e-1526.52Lanosterol 14-alpha demethylase OS=Rattus norvegic... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS02_01O03_f Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa01O03, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF832451 ID=CF832451; Name=CF832451; organism=Citrus sinensis; type=EST; length=866bp
TTTTTTTTTTTTTTACCCAGCATTAATGCTCTAATTCTAATCATGGTATT
ATAAAATTTTCCAATGTGGGGAAACATCTTTACTCTAAATAAAACTAGAG
TTGTGTTGAGCTCAATTTAAAAATCTAAACAGCTCTTGTCTAAGCTTTGA
ATCTCAATACAAAATTAAATACATTAAACTTCATTATAAAAGAGTCTACA
TTGGCATCATGCTAATCTTATTGACAATATATAGTTGCCGGTATTAATTA
TTTTTGCACCTTAGTTTCCGTAGAATCAATTGGGTACCCAGTTCCGGATG
AAGGGTTGGAACCGCCGTTGGAGCATGGACATAGGATGGAGACAACTCGA
ATGAGTAGTTTTGAAGAATCATTGCCATGGCCATTTTTGCTTCTACTAGA
GCAAAATTTTGTCCAACACATATCCTAGGACCCAATGCGAATGGGAAATA
TGAAACTTGATTTTTGGCCGCCTTTGAAATTCCTTCCGCAAACCTTTCCG
GCTTAAACTCAACTGCATCGTAACCCCAAAGTTCAGGATCCTGGTGAACT
AGGATCATTGGCAAGCCAATTTGAACCCCAGCGGGTAATGTCAAGTTTCC
GAGTTTCGTTTCCTTAAGAACAGATCGACTAAGCACAGTTACAGGCGGAT
AAAGCCTGAGTACCTCGTAAAGTATCATCTGGACAATTTTGAGGTGATTC
AATCCATCATAGTTGGGCTTATTATTGCCAAACACTTGCAAAACCTCTTG
CCTTGCACGTTCTTGCCAATCTTGATGCTTGCTCAATAAGACCATCGTCC
AATTAAGCAAAACAGAGGTAGTCTCTTGGCCGGCAAAGTAAAATAGCTTA
CACTCTTCGATCACAT
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