CF832451

Overview
NameCF832451
Unique NameCF832451
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length866
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CF832451 vs. ExPASy Swiss-Prot
Match: CP4V3_MOUSE (Cytochrome P450 4V3 OS=Mus musculus GN=Cyp4v3 PE=1 SV=1)

HSP 1 Score: 106.301 bits (264), Expect = 2.322e-22
Identity = 58/173 (33.53%), Postives = 102/173 (58.96%), Query Frame = -3
Query:  346 EECKLFYFAGQETTSVLLNWTMVLLSKHQDWQERARQEVLQVFG-NNKP-NYDGLNHLKIVQMILYEVLRLYPPVTVLSRSVLKETKLGNLTLPAGVQIGLPMILVHQDPELWGYDAVEFKPERFAEGISKAAKNQVSYFPFALGPRICVGQNFALVEAKMAMAMILQNYSFE 858
            EE   F F G +TT+  +NW++ LL  + + Q +  QE+ +VFG +++P   + L  LK +  ++ E LR++P V + +RS+ ++ ++G   +  G +  +    +H+DP  +  D  EF+PERF    S+  ++  +Y PF+ GPR C+GQ FA++E K  +A IL+ +  E
Sbjct:  321 EEVDTFMFEGHDTTAAAINWSLYLLGTNPEVQRKVDQELDEVFGRSHRPVTLEDLKKLKYLDCVIKETLRVFPSVPLFARSLSEDCEVGGYKVTKGTEAIIIPYALHRDPRYFP-DPEEFRPERFFPENSQG-RHPYAYVPFSAGPRNCIGQKFAVMEEKTILACILRQFWVE 491          
BLAST of CF832451 vs. ExPASy Swiss-Prot
Match: CP4AL_PIG (Taurochenodeoxycholic 6 alpha-hydroxylase OS=Sus scrofa GN=CYP4A21 PE=1 SV=1)

HSP 1 Score: 106.301 bits (264), Expect = 2.322e-22
Identity = 63/200 (31.50%), Postives = 108/200 (54.00%), Query Frame = -3
Query:  262 ECKLFYFAGQETTSVLLNWTMVLLSKHQDWQERARQEVLQVFGNNKP-NYDGLNHLKIVQMILYEVLRLYPPVTVLSRSVLKE-TKLGNLTLPAGVQIGLPMILVHQDPELWGYDAVEFKPERFAEGISKAAKNQVSYFPFALGPRICVGQNFALVEAKMAMAMILQNYSFELSPSYVHAPTAVPTLHPELGTQLILRKL 855
            E   F  AG ++T+  ++W +  L+ + + Q+R R+E+  + G+     +D L+ +    M + E LRLYPPV  + R + K  T     +LPAG+ + L +  +H +P++W  +  EF P RFA G   +A++  ++ PF+ G R C+G+ FA+ E K+ +A+ L  +     PS +  P     L  + G  L LRK+
Sbjct:  308 EVDTFMAAGHDSTASGISWVLYALASNPEHQQRCREEIQGLLGDGTSITWDHLDQMPYTTMCIKEALRLYPPVPSVGRELSKPITFPDGRSLPAGIILSLSIYGLHHNPQVWP-NPEEFDPSRFAPG---SARHSHAFMPFSGGSRNCIGKQFAMNEMKVVVALTLLRFELAPDPSRIPVPIQGIVLKSKNGIHLNLRKI 503          
BLAST of CF832451 vs. ExPASy Swiss-Prot
Match: C4D20_DROME (Probable cytochrome P450 4d20 OS=Drosophila melanogaster GN=Cyp4d20 PE=3 SV=1)

HSP 1 Score: 106.301 bits (264), Expect = 2.322e-22
Identity = 60/177 (33.90%), Postives = 99/177 (55.93%), Query Frame = -3
Query:  337 EECKLFYFAGQETTSVLLNWTMVLLSKHQDWQERARQEVLQVFGNNKP---NYDGLNHLKIVQMILYEVLRLYPPVTVLSRSVLKETKLGNLTLPAGVQIGLPMILVHQDPELWGYDAVEFKPERFAEGISKAAKNQVSYFPFALGPRICVGQNFALVEAKMAMAMILQNYSFELSP 858
            EE   F F G +TTS  ++  +  +++H + Q+R  +E+ +V G +         L  LK +  ++ E +RLYPPV  + R   KE ++G+ T+PA   I L +   H+D   +  D + F+PERF E   +   N  +Y PF+ GP+ C+GQ FA++E K+ ++ +L+ Y  EL P
Sbjct:  308 EEVDTFMFEGDDTTSSGVSHALYAIARHPEVQQRIFEELQRVLGPDASAPVTQAQLQDLKYLDCVIKETMRLYPPVPAIGRHAQKELEIGDKTIPANTSIYLVLYYAHRDANYFP-DPLSFRPERFLED-QEQGHNTFAYVPFSAGPKNCIGQKFAVLEMKVLISKVLRFY--ELLP 480          
BLAST of CF832451 vs. ExPASy Swiss-Prot
Match: CP4FC_HUMAN (Cytochrome P450 4F12 OS=Homo sapiens GN=CYP4F12 PE=1 SV=1)

HSP 1 Score: 105.916 bits (263), Expect = 3.033e-22
Identity = 58/177 (32.77%), Postives = 97/177 (54.80%), Query Frame = -3
Query:  349 ECKLFYFAGQETTSVLLNWTMVLLSKHQDWQERARQEVLQVFGNNKP---NYDGLNHLKIVQMILYEVLRLYPPVTVLSRSVLKETKL-GNLTLPAGVQIGLPMILVHQDPELWG----YDAVEFKPERFAEGISKAAKNQVSYFPFALGPRICVGQNFALVEAKMAMAMILQNYSF 855
            E   F F G +TT+  L+W +  L++H ++QER RQEV ++  +  P    +D L  L  + M + E LRL+PP   +SR   ++  L     +P G+   + +I VH +P +W     YD   F PE      +   ++ +++ PF+ GPR C+GQ FA+ E K+ +A++L ++ F
Sbjct:  321 EADTFMFGGHDTTASGLSWVLYNLARHPEYQERCRQEVQELLKDRDPKEIEWDDLAQLPFLTMCVKESLRLHPPAPFISRCCTQDIVLPDGRVIPKGITCLIDIIGVHHNPTVWPDPEVYDPFRFDPE------NSKGRSPLAFIPFSAGPRNCIGQAFAMAEMKVVLALMLLHFRF 491          
BLAST of CF832451 vs. ExPASy Swiss-Prot
Match: CP56_YEAST (Cytochrome P450-DIT2 OS=Saccharomyces cerevisiae GN=DIT2 PE=1 SV=2)

HSP 1 Score: 105.531 bits (262), Expect = 3.961e-22
Identity = 64/189 (33.86%), Postives = 102/189 (53.97%), Query Frame = -3
Query:  295 AGQETTSVLLNWTMVLLSKHQ-DWQERARQEVLQVFGNNKPNYDGLNHLKIVQMILYEVLRLYPPV-TVLSRSVLKETKLG-NLTLPAGVQIGLPMILVHQDPELWGYDAVEFKPERFAEGIS------KAAKNQVSYFPFALGPRICVGQNFALVEAKMAMAMILQNYSFELSPSYVHAPTAVPTLHP 834
            AG E   +L N ++ LL+K+  +WQE+ R+EV     N   +  GL  L ++   L+EV+R+YPP+ T+++R   K  KLG  + +P GV +G        DP+ WG  A +FKPER+   I       + AKN+ +   F  G R C+G+  AL E ++++A +L+ + + L P +    T    L P
Sbjct:  293 AGHENPQLLFNSSLYLLAKYSNEWQEKLRKEV-----NGITDPKGLADLPLLNAFLFEVVRMYPPLSTIINRCTTKTCKLGAEIVIPKGVYVGYNNFGTSHDPKTWGTTADDFKPERWGSDIETIRKNWRMAKNRCAVTGFHGGRRACLGEKLALTEMRISLAEMLKQFRWSLDPEWEEKLTPAGPLCP 476          
BLAST of CF832451 vs. ExPASy Swiss-Prot
Match: CP4F3_RAT (Leukotriene-B(4) omega-hydroxylase 2 OS=Rattus norvegicus GN=Cyp4f3 PE=2 SV=1)

HSP 1 Score: 105.531 bits (262), Expect = 3.961e-22
Identity = 59/171 (34.50%), Postives = 95/171 (55.56%), Query Frame = -3
Query:  355 ECKLFYFAGQETTSVLLNWTMVLLSKHQDWQERARQEVLQVFGNNKP---NYDGLNHLKIVQMILYEVLRLYPPVTVLSRSVLKETKL-GNLTLPAGVQIGLPMILVHQDPELWGYDAVEFKPERFAEGISKAAKNQVSYFPFALGPRICVGQNFALVEAKMAMAMILQNY 855
            E   F F G +TT+  L+W +  L+KH ++QER RQEV ++  + +P    +D L  L  + M + E LRL+PP T +SR   ++  L     +P GV   + +   H +P +W  D   + P RF     K  ++ +++ PF+ GPR C+GQ FA+ E K+A+A+ L  +
Sbjct:  321 EADTFMFGGHDTTASGLSWILYNLAKHPEYQERCRQEVRELLRDREPEEIEWDDLAQLPFLTMCIKESLRLHPPATAISRCCTQDIMLPDGRVIPKGVICRISIFGTHHNPAVWP-DPEVYNPFRFDADNGK-GRSPLAFIPFSAGPRNCIGQTFAMSEMKVALALTLLRF 489          
BLAST of CF832451 vs. ExPASy Swiss-Prot
Match: CP4AA_MOUSE (Cytochrome P450 4A10 OS=Mus musculus GN=Cyp4a10 PE=2 SV=1)

HSP 1 Score: 105.531 bits (262), Expect = 3.961e-22
Identity = 66/200 (33.00%), Postives = 104/200 (52.00%), Query Frame = -3
Query:  262 ECKLFYFAGQETTSVLLNWTMVLLSKHQDWQERARQEVLQVFGNNKP-NYDGLNHLKIVQMILYEVLRLYPPVTVLSRSVLKETKLGN-LTLPAGVQIGLPMILVHQDPELWGYDAVEFKPERFAEGISKAAKNQVSYFPFALGPRICVGQNFALVEAKMAMAMILQNYSFELSPSYVHAPTAVPTLHPELGTQLILRKL 855
            E   F F G +TT+  ++W    L+ H D Q+R R+EV  + G+     +D L+ +    M + E LRLYPPV  + R +       +  +LP GVQ+ L +  +H +P++W    V F P RFA     + ++  S+ PF+ G R C+G+ FA+ E K+ +A+ L  +     P+ V  P A   L  + G  L L+KL
Sbjct:  313 EVDTFMFEGHDTTASGVSWIFYALATHPDHQQRCREEVQSLLGDGSSITWDHLDQIPYTTMCIKEALRLYPPVPGIVRELSTSVTFPDGRSLPKGVQVTLSIYGLHHNPKVWPNPEV-FDPSRFAPD---SPRHSHSFLPFSGGARNCIGKQFAMSELKVIVALTLLRFELLPDPTRVPMPLARLVLKSKNGIYLHLKKL 508          
BLAST of CF832451 vs. ExPASy Swiss-Prot
Match: CP3AP_MOUSE (Cytochrome P450 3A25 OS=Mus musculus GN=Cyp3a25 PE=2 SV=1)

HSP 1 Score: 105.531 bits (262), Expect = 3.961e-22
Identity = 56/168 (33.33%), Postives = 95/168 (56.55%), Query Frame = -3
Query:  346 LFYFAGQETTSVLLNWTMVLLSKHQDWQERARQEVLQVFGNNKP-NYDGLNHLKIVQMILYEVLRLYPPVTVLSRSVLKETKLGNLTLPAGVQIGLPMILVHQDPELWGYDAVEFKPERFAEGISKAAKNQVSYFPFALGPRICVGQNFALVEAKMAMAMILQNYSFE 846
            +F F G + TS  ++  M  L+ H D Q++ + E+ +   N  P  YD L  ++ + M++ E LRLYP    L R   K+ ++  + +P G  + +P+  +H++PE W  +  EF PERF++  +K   +   Y PF  GPR C+G  FAL+  K+A+  +LQN++ +
Sbjct:  301 IFIFGGYDATSTSISLIMYELATHPDVQKKLQDEIDRTLPNKAPVTYDALMDMEYLDMVVNESLRLYPIAIRLERVSKKDVEINGVFIPKGTVVMIPIYPLHRNPEYWP-EPQEFCPERFSKE-NKGNIDPYIYMPFGNGPRNCIGMRFALISIKLAVIGVLQNFTVQ 466          
BLAST of CF832451 vs. ExPASy Swiss-Prot
Match: CP4FB_HUMAN (Cytochrome P450 4F11 OS=Homo sapiens GN=CYP4F11 PE=2 SV=2)

HSP 1 Score: 105.145 bits (261), Expect = 5.173e-22
Identity = 64/197 (32.49%), Postives = 105/197 (53.30%), Query Frame = -3
Query:  289 ECKLFYFAGQETTSVLLNWTMVLLSKHQDWQERARQEVLQVFGNNKP---NYDGLNHLKIVQMILYEVLRLYPPVTVLSRSVLKETKL-GNLTLPAGVQIGLPMILVHQDPELWG----YDAVEFKPERFAEGISKAAKNQVSYFPFALGPRICVGQNFALVEAKMAMAMILQNYSFELSPSYVHAPTAVPTLHPEL 855
            E   F F G +TT+  L+W +  L+KH ++QE+ RQEV ++  + +P    +D L  L  + M + E LRL+PPV V+SR   ++  L     +P G+   + +I +H +P +W     YD   F  E   E      ++ +++ PF+ GPR C+GQ FA+ E K+ +A+ L +  F + P++       P   PEL
Sbjct:  321 EADTFMFEGHDTTASGLSWVLYHLAKHPEYQEQCRQEVQELLKDREPIEIEWDDLAQLPFLTMCIKESLRLHPPVPVISRCCTQDFVLPDGRVIPKGIVCLINIIGIHYNPTVWPDPEVYDPFRFNQENIKE------RSPLAFIPFSAGPRNCIGQAFAMAEMKVVLALTLLH--FRILPTHTE-----PRRKPEL 504          
BLAST of CF832451 vs. ExPASy Swiss-Prot
Match: CP4Z1_HUMAN (Cytochrome P450 4Z1 OS=Homo sapiens GN=CYP4Z1 PE=2 SV=1)

HSP 1 Score: 104.76 bits (260), Expect = 6.757e-22
Identity = 62/184 (33.70%), Postives = 104/184 (56.52%), Query Frame = -3
Query:  310 ECKLFYFAGQETTSVLLNWTMVLLSKHQDWQERARQEVLQVFGNNKP-NYDGLNHLKIVQMILYEVLRLYPPVTVLSRSVLKE-TKLGNLTLPAGVQIGLPMILVHQDPELWGYDAVEFKPERFAEGISKAAKNQVSYFPFALGPRICVGQNFALVEAKMAMAMILQNYSFELSPSYVHAPTAV 855
            E K F FAG +TTS  ++W +  L+K+ + Q+R R E+ ++ G+     ++ L+ +    M + E LRLY PV  +SR + K  T     +LPAG+ + + +  +H +P  W  D   F P RF+   S+   +  ++ PF+ G R C+GQ+FA++E K+A+A+ L    F+L+P +   P  V
Sbjct:  307 EVKTFMFAGHDTTSSAISWILYCLAKYPEHQQRCRDEIRELLGDGSSITWEHLSQMPYTTMCIKECLRLYAPVVNISRLLDKPITFPDGRSLPAGITVFINIWALHHNPYFW-EDPQVFNPLRFSRENSEKI-HPYAFIPFSAGLRNCIGQHFAIIECKVAVALTL--LRFKLAPDHSRPPQPV 486          
The following BLAST results are available for this feature:
BLAST of CF832451 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 457
Match NameE-valueIdentityDescription
CP4V3_MOUSE2.322e-2233.53Cytochrome P450 4V3 OS=Mus musculus GN=Cyp4v3 PE=1... [more]
CP4AL_PIG2.322e-2231.50Taurochenodeoxycholic 6 alpha-hydroxylase OS=Sus s... [more]
C4D20_DROME2.322e-2233.90Probable cytochrome P450 4d20 OS=Drosophila melano... [more]
CP4FC_HUMAN3.033e-2232.77Cytochrome P450 4F12 OS=Homo sapiens GN=CYP4F12 PE... [more]
CP56_YEAST3.961e-2233.86Cytochrome P450-DIT2 OS=Saccharomyces cerevisiae G... [more]
CP4F3_RAT3.961e-2234.50Leukotriene-B(4) omega-hydroxylase 2 OS=Rattus nor... [more]
CP4AA_MOUSE3.961e-2233.00Cytochrome P450 4A10 OS=Mus musculus GN=Cyp4a10 PE... [more]
CP3AP_MOUSE3.961e-2233.33Cytochrome P450 3A25 OS=Mus musculus GN=Cyp3a25 PE... [more]
CP4FB_HUMAN5.173e-2232.49Cytochrome P450 4F11 OS=Homo sapiens GN=CYP4F11 PE... [more]
CP4Z1_HUMAN6.757e-2233.70Cytochrome P450 4Z1 OS=Homo sapiens GN=CYP4Z1 PE=2... [more]

Pages

back to top
Properties
Property NameValue
Genbank descriptionUCRCS02_01O03_f Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa01O03, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF832451 ID=CF832451; Name=CF832451; organism=Citrus sinensis; type=EST; length=866bp
TTTTTTTTTTTTTTACCCAGCATTAATGCTCTAATTCTAATCATGGTATT
ATAAAATTTTCCAATGTGGGGAAACATCTTTACTCTAAATAAAACTAGAG
TTGTGTTGAGCTCAATTTAAAAATCTAAACAGCTCTTGTCTAAGCTTTGA
ATCTCAATACAAAATTAAATACATTAAACTTCATTATAAAAGAGTCTACA
TTGGCATCATGCTAATCTTATTGACAATATATAGTTGCCGGTATTAATTA
TTTTTGCACCTTAGTTTCCGTAGAATCAATTGGGTACCCAGTTCCGGATG
AAGGGTTGGAACCGCCGTTGGAGCATGGACATAGGATGGAGACAACTCGA
ATGAGTAGTTTTGAAGAATCATTGCCATGGCCATTTTTGCTTCTACTAGA
GCAAAATTTTGTCCAACACATATCCTAGGACCCAATGCGAATGGGAAATA
TGAAACTTGATTTTTGGCCGCCTTTGAAATTCCTTCCGCAAACCTTTCCG
GCTTAAACTCAACTGCATCGTAACCCCAAAGTTCAGGATCCTGGTGAACT
AGGATCATTGGCAAGCCAATTTGAACCCCAGCGGGTAATGTCAAGTTTCC
GAGTTTCGTTTCCTTAAGAACAGATCGACTAAGCACAGTTACAGGCGGAT
AAAGCCTGAGTACCTCGTAAAGTATCATCTGGACAATTTTGAGGTGATTC
AATCCATCATAGTTGGGCTTATTATTGCCAAACACTTGCAAAACCTCTTG
CCTTGCACGTTCTTGCCAATCTTGATGCTTGCTCAATAAGACCATCGTCC
AATTAAGCAAAACAGAGGTAGTCTCTTGGCCGGCAAAGTAAAATAGCTTA
CACTCTTCGATCACAT
back to top