CF832451

Overview
NameCF832451
Unique NameCF832451
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length866
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CF832451 vs. ExPASy Swiss-Prot
Match: CYPE_BACSU (Probable bifunctional P-450/NADPH-P450 reductase 2 OS=Bacillus subtilis GN=cypE PE=2 SV=1)

HSP 1 Score: 102.834 bits (255), Expect = 2.568e-21
Identity = 56/164 (34.15%), Postives = 92/164 (56.10%), Query Frame = -3
Query:  355 FYFAGQETTSVLLNWTMVLLSKHQDWQERARQEVLQVFGNNKPNYDGLNHLKIVQMILYEVLRLYPPVTVLSRSVLKETKL-GNLTLPAGVQIGLPMILVHQDPELWGYDAVEFKPERFAEGISKAAKNQVSYFPFALGPRICVGQNFALVEAKMAMAMILQNY 843
            F  AG ETTS LL++ +  L  H +  ++A++E  +V  ++ P Y  +  LK ++M+L E LRLYP     S    ++T L G   +  G  + + +  +H+D   WG DA +F+PERF +    ++    +Y PF  G R C+G  FAL EA M + ++L+++
Sbjct:  264 FLIAGHETTSGLLSFAIYCLLTHPEKLKKAQEEADRVLTDDTPEYKQIQQLKYIRMVLNETLRLYPTAPAFSLYAKEDTVLGGEYPISKGQPVTVLIPKLHRDQNAWGPDAEDFRPERFED---PSSIPHHAYKPFGNGQRACIGMQFALQEATMVLGLVLKHF 424          
BLAST of CF832451 vs. ExPASy Swiss-Prot
Match: CP4V3_RAT (Cytochrome P450 4V3 OS=Rattus norvegicus GN=Cyp4v3 PE=2 SV=1)

HSP 1 Score: 102.834 bits (255), Expect = 2.568e-21
Identity = 56/173 (32.37%), Postives = 100/173 (57.80%), Query Frame = -3
Query:  346 EECKLFYFAGQETTSVLLNWTMVLLSKHQDWQERARQEVLQVFG-NNKP-NYDGLNHLKIVQMILYEVLRLYPPVTVLSRSVLKETKLGNLTLPAGVQIGLPMILVHQDPELWGYDAVEFKPERFAEGISKAAKNQVSYFPFALGPRICVGQNFALVEAKMAMAMILQNYSFE 858
            EE   F F G +TT+  +NW++ LL  + + Q +  +E+  VFG +++P   + L  LK +  ++ E LR++P V + +RS+ ++ ++    +  G +  +    +H+DP  +  D  EF+PERF    S+  ++  +Y PF+ GPR C+GQ FA++E K  +A IL+ +  E
Sbjct:  321 EEVDTFMFEGHDTTAAAINWSLYLLGSNPEVQRKVDKELDDVFGRSHRPVTLEDLKKLKYLDCVIKETLRVFPSVPLFARSLSEDCEVAGYKISKGTEAVIIPYALHRDPRYFP-DPEEFQPERFFPENSQG-RHPYAYVPFSAGPRNCIGQKFAVMEEKTILACILREFWIE 491          
BLAST of CF832451 vs. ExPASy Swiss-Prot
Match: CP4F1_RAT (Cytochrome P450 4F1 OS=Rattus norvegicus GN=Cyp4f1 PE=2 SV=1)

HSP 1 Score: 102.834 bits (255), Expect = 2.568e-21
Identity = 58/175 (33.14%), Postives = 96/175 (54.86%), Query Frame = -3
Query:  355 ECKLFYFAGQETTSVLLNWTMVLLSKHQDWQERARQEVLQVFGNNKPN---YDGLNHLKIVQMILYEVLRLYPPVTVLSRSVLKETKLGN-LTLPAGVQIGLPMILVHQDPELWG----YDAVEFKPERFAEGISKAAKNQVSYFPFALGPRICVGQNFALVEAKMAMAMILQNY 855
            E   F F G +TT+  L+W +  L+   ++QER RQEV ++  +  P    +D L  L  + M + E LRL+PPVTV+SR   ++  L +  T+P G+   + +  +H +P +W     Y+   F PE   +       + +++ PF+ GPR C+GQ FA+ E K+A+A+ L  +
Sbjct:  321 EADTFMFEGHDTTASGLSWILYNLANDPEYQERCRQEVQELLRDRDPEEIEWDDLAQLPFLTMCIKESLRLHPPVTVISRCCTQDILLPDGRTIPKGIICLISIFGIHHNPSVWPDPEVYNPFRFDPENIKDS------SPLAFIPFSAGPRNCIGQTFAMSEMKVALALTLLRF 489          
BLAST of CF832451 vs. ExPASy Swiss-Prot
Match: CP4D8_DROME (Cytochrome P450 4d8 OS=Drosophila melanogaster GN=Cyp4d8 PE=2 SV=2)

HSP 1 Score: 102.834 bits (255), Expect = 2.568e-21
Identity = 71/207 (34.30%), Postives = 110/207 (53.14%), Query Frame = -3
Query:  265 EECKLFYFAGQETTSVLLNWTMVLLSKHQDWQERARQEVLQVFGNNKP---NYDGLNHLKIVQMILYEVLRLYPPVTVLSRSVLKETKL-----GNLTLPAGVQIGLPMILVHQDPELWGYDAVEFKPERFAEGISKAAKNQVSYFPFALGPRICVGQNFALVEAKMAMAMILQNYSFELSPSYVHAPTAVP-TLHPELGTQLILRK 858
            EE   F F G +TT+  L++ +  LS+H + Q +  +E++QV G ++    +   L  LK ++ ++ E LR+YPPV ++ R +  + K      G+  +PAG +I + +  VH+ PE +  +  EF PER   G   A    +   PF+ GPR C+GQ FA +E KM +A I++ Y  EL P        V   L  E G QL +RK
Sbjct:  259 EEVDTFMFEGHDTTTSALSFCLHELSRHPEVQAKMLEEIVQVLGTDRSRPVSIRDLGELKYMECVIKESLRMYPPVPIVGRKLQTDFKYTHSVHGDGVIPAGSEIIIGIFGVHRQPETFP-NPDEFIPERHENGSRVAPFKMI---PFSAGPRNCIGQKFAQLEMKMMLAKIVREY--ELLPMGQRVECIVNIVLRSETGFQLGMRK 459          
BLAST of CF832451 vs. ExPASy Swiss-Prot
Match: CP4D2_DROME (Cytochrome P450 4d2 OS=Drosophila melanogaster GN=Cyp4d2 PE=2 SV=2)

HSP 1 Score: 102.834 bits (255), Expect = 2.568e-21
Identity = 56/177 (31.64%), Postives = 103/177 (58.19%), Query Frame = -3
Query:  337 EECKLFYFAGQETTSVLLNWTMVLLSKHQDWQERARQEVLQVFGNNKPN---YDGLNHLKIVQMILYEVLRLYPPVTVLSRSVLKETKLGNLTLPAGVQIGLPMILVHQDPELWGYDAVEFKPERFAEGISKAAKNQVSYFPFALGPRICVGQNFALVEAKMAMAMILQNYSFELSP 858
            EE   F F G +TT+  +++ +  +S+H + Q+R +QE+  V G ++ +      L  LK ++ ++ E LRL+PPV ++ R   ++ ++    +PAG    + + ++ +DPE +     EF+PERF   + +   +  +Y PF+ GPR C+GQ FA++E K  ++ +L++  FEL P
Sbjct:  303 EEVDTFMFEGHDTTTSAISFCLYEISRHPEVQQRLQQEIRDVLGEDRKSPVTLRDLGELKFMENVIKESLRLHPPVPMIGRWFAEDVEIRGKHIPAGTNFTMGIFVLLRDPEYFE-SPDEFRPERFDADVPQI--HPYAYIPFSAGPRNCIGQKFAMLEMKSTVSKLLRH--FELLP 474          
BLAST of CF832451 vs. ExPASy Swiss-Prot
Match: CP4A6_RABIT (Cytochrome P450 4A6 OS=Oryctolagus cuniculus GN=CYP4A6 PE=1 SV=1)

HSP 1 Score: 102.834 bits (255), Expect = 2.568e-21
Identity = 68/203 (33.50%), Postives = 105/203 (51.72%), Query Frame = -3
Query:  259 ECKLFYFAGQETTSVLLNWTMVLLSKHQDWQERARQEVLQVFGNNKP-NYDGLNHLKIVQMILYEVLRLYPPVTVLSRSVLKETKLGN-LTLPAGVQIGLPMILVHQDPELWGYDAVEFKPERFAEGISKAAKNQVSYFPFALGPRICVGQNFALVEAKMAMAMILQNYSFELSPSYVHAPTAVP--TLHPELGTQLILRKLR 855
            E   F F G +TT+  ++W    L+ H + Q R R+E+  + G+     ++ L+ +    M + E LRLYPPV  + R +       +  +LP GV + L +  +H +P++W    V F P  FA G   +A++  ++ PF+ GPR C+G+ FA+ E K+A+A+ L    FEL P     P   P   L    G  L LRKLR
Sbjct:  314 EVDTFMFEGHDTTASGISWIFYALATHPEHQHRCREEIQGLLGDGASITWEHLDQMPYTTMCIKEALRLYPPVPGVGRQLSSPVTFPDGRSLPKGVIVTLSIYALHHNPKVWPNPEV-FDPFPFAPG---SARHSHAFLPFSGGPRNCIGKQFAMNELKVAVALTL--VRFELLPDPKRVPDQKPRLVLKSSNGIHLRLRKLR 510          
BLAST of CF832451 vs. ExPASy Swiss-Prot
Match: CP4F6_RAT (Cytochrome P450 4F6 OS=Rattus norvegicus GN=Cyp4f6 PE=2 SV=1)

HSP 1 Score: 102.064 bits (253), Expect = 4.380e-21
Identity = 57/171 (33.33%), Postives = 97/171 (56.73%), Query Frame = -3
Query:  355 ECKLFYFAGQETTSVLLNWTMVLLSKHQDWQERARQEVLQVFGNNKP---NYDGLNHLKIVQMILYEVLRLYPPVTVLSRSVLKETKL-GNLTLPAGVQIGLPMILVHQDPELWGYDAVEFKPERFAEGISKAAKNQVSYFPFALGPRICVGQNFALVEAKMAMAMILQNY 855
            E   F F G +TT+  L+W +  L++H ++QER RQEV ++  + +P    +D L  L  + M + E LRL+PPV ++SR   ++  L     +P G    + +  VH +P +W  D   + P RF +  +   ++ +++ PF+ GPR C+GQ FA+ E K+A+A+ L  +
Sbjct:  321 EADTFMFGGHDTTASALSWILYNLARHPEYQERCRQEVRELLRDREPEEIEWDDLAQLPFLTMCIKESLRLHPPVLLISRCCSQDIVLPDGRVIPKGNICVISIFGVHHNPSVWP-DPEVYNPFRF-DPENPQKRSPLAFIPFSAGPRNCIGQTFAMSEIKVALALTLLRF 489          
BLAST of CF832451 vs. ExPASy Swiss-Prot
Match: CP4AB_HUMAN (Cytochrome P450 4A11 OS=Homo sapiens GN=CYP4A11 PE=1 SV=1)

HSP 1 Score: 102.064 bits (253), Expect = 4.380e-21
Identity = 63/200 (31.50%), Postives = 105/200 (52.50%), Query Frame = -3
Query:  262 ECKLFYFAGQETTSVLLNWTMVLLSKHQDWQERARQEVLQVFGNNKP-NYDGLNHLKIVQMILYEVLRLYPPVTVLSRSVLKETKLGN-LTLPAGVQIGLPMILVHQDPELWGYDAVEFKPERFAEGISKAAKNQVSYFPFALGPRICVGQNFALVEAKMAMAMILQNYSFELSPSYVHAPTAVPTLHPELGTQLILRKL 855
            E   F F G +TT+  ++W +  L+ H   QER R+E+  + G+     ++ L+ +    M + E LRLYPPV  + R +       +  +LP G+ + L +  +H +P++W    V F P RFA G   +A++  ++ PF+ G R C+G+ FA+ E K+A A+ L  +     P+ +  P A   L  + G  L LR+L
Sbjct:  314 EVDTFMFEGHDTTASGISWILYALATHPKHQERCREEIHSLLGDGASITWNHLDQMPYTTMCIKEALRLYPPVPGIGRELSTPVTFPDGRSLPKGIMVLLSIYGLHHNPKVWPNPEV-FDPFRFAPG---SAQHSHAFLPFSGGSRNCIGKQFAMNELKVATALTLLRFELLPDPTRIPIPIARLVLKSKNGIHLRLRRL 509          
BLAST of CF832451 vs. ExPASy Swiss-Prot
Match: C4D21_DROME (Probable cytochrome P450 4d21 OS=Drosophila melanogaster GN=Cyp4d21 PE=3 SV=1)

HSP 1 Score: 102.064 bits (253), Expect = 4.380e-21
Identity = 59/180 (32.78%), Postives = 104/180 (57.78%), Query Frame = -3
Query:  337 EECKLFYFAGQETTSVLLNWTMVLLSKHQDWQERARQEVLQVFGNNKPNYDG------LNHLKIVQMILYEVLRLYPPVTVLSRSVLKETKLGNLTLPAGVQIGLPMILVHQDPELWGYDAVEFKPERFAEGISKAAKNQVSYFPFALGPRICVGQNFALVEAKMAMAMILQNYSFELSP 858
            EE  +F FAG +TT+  ++  +  +S+H   QE   +E++ V G   P+ D       L  LK +  ++ E +RL+PPV +L R + ++ K+G +T+P    I L    V++DPE +  D + FKPER+ +  + +    ++Y PF+ GP+ C+GQ FA ++ K  ++ ++++Y  EL P
Sbjct:  308 EEVDVFIFAGDDTTTSGVSHALHAISRHPKVQECIYEELVSVLG---PDPDASVTQTKLLELKYLDCVIKETMRLHPPVPILGRYIPEDLKIGEITIPGNTSILLMPYYVYRDPEYFP-DPLVFKPERWMDMKTTSNTPPLAYIPFSSGPKNCIGQKFANLQMKALISKVIRHY--ELLP 481          
BLAST of CF832451 vs. ExPASy Swiss-Prot
Match: CP4A2_RAT (Cytochrome P450 4A2 OS=Rattus norvegicus GN=Cyp4a2 PE=1 SV=2)

HSP 1 Score: 101.679 bits (252), Expect = 5.720e-21
Identity = 65/203 (32.02%), Postives = 105/203 (51.72%), Query Frame = -3
Query:  259 ECKLFYFAGQETTSVLLNWTMVLLSKHQDWQERARQEVLQVFGNNKP-NYDGLNHLKIVQMILYEVLRLYPPVTVLSRSVLKETKLGN-LTLPAGVQIGLPMILVHQDPELWGYDAVEFKPERFAEGISKAAKNQVSYFPFALGPRICVGQNFALVEAKMAMAMILQNYSFELSPSYVHAPTAVP--TLHPELGTQLILRKLR 855
            E   F F G +TT+  ++W    L+ H + QER R+EV  + G+     +D L+ +    M + E LRLY PV  +SR +       +  ++P G+++ + +  +H +P  W    V F P RF+     + ++  +Y PF+ G R C+G+ FA+ E K+A+A+ L    FEL P     P  +P   L  + G  L L+KLR
Sbjct:  308 EVDTFMFEGHDTTASGISWVFYALATHPEHQERCREEVQSILGDGTSVTWDHLDQMPYTTMCIKEALRLYSPVPSVSRELSSPVTFPDGRSIPKGIRVTILIYGLHHNPSYWPNPKV-FDPSRFSPD---SPRHSHAYLPFSGGARNCIGKQFAMNELKVAVALTL--LRFELLPDPTRIPVPMPRLVLKSKNGIHLRLKKLR 504          
The following BLAST results are available for this feature:
BLAST of CF832451 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 457
Match NameE-valueIdentityDescription
CYPE_BACSU2.568e-2134.15Probable bifunctional P-450/NADPH-P450 reductase 2... [more]
CP4V3_RAT2.568e-2132.37Cytochrome P450 4V3 OS=Rattus norvegicus GN=Cyp4v3... [more]
CP4F1_RAT2.568e-2133.14Cytochrome P450 4F1 OS=Rattus norvegicus GN=Cyp4f1... [more]
CP4D8_DROME2.568e-2134.30Cytochrome P450 4d8 OS=Drosophila melanogaster GN=... [more]
CP4D2_DROME2.568e-2131.64Cytochrome P450 4d2 OS=Drosophila melanogaster GN=... [more]
CP4A6_RABIT2.568e-2133.50Cytochrome P450 4A6 OS=Oryctolagus cuniculus GN=CY... [more]
CP4F6_RAT4.380e-2133.33Cytochrome P450 4F6 OS=Rattus norvegicus GN=Cyp4f6... [more]
CP4AB_HUMAN4.380e-2131.50Cytochrome P450 4A11 OS=Homo sapiens GN=CYP4A11 PE... [more]
C4D21_DROME4.380e-2132.78Probable cytochrome P450 4d21 OS=Drosophila melano... [more]
CP4A2_RAT5.720e-2132.02Cytochrome P450 4A2 OS=Rattus norvegicus GN=Cyp4a2... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS02_01O03_f Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa01O03, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF832451 ID=CF832451; Name=CF832451; organism=Citrus sinensis; type=EST; length=866bp
TTTTTTTTTTTTTTACCCAGCATTAATGCTCTAATTCTAATCATGGTATT
ATAAAATTTTCCAATGTGGGGAAACATCTTTACTCTAAATAAAACTAGAG
TTGTGTTGAGCTCAATTTAAAAATCTAAACAGCTCTTGTCTAAGCTTTGA
ATCTCAATACAAAATTAAATACATTAAACTTCATTATAAAAGAGTCTACA
TTGGCATCATGCTAATCTTATTGACAATATATAGTTGCCGGTATTAATTA
TTTTTGCACCTTAGTTTCCGTAGAATCAATTGGGTACCCAGTTCCGGATG
AAGGGTTGGAACCGCCGTTGGAGCATGGACATAGGATGGAGACAACTCGA
ATGAGTAGTTTTGAAGAATCATTGCCATGGCCATTTTTGCTTCTACTAGA
GCAAAATTTTGTCCAACACATATCCTAGGACCCAATGCGAATGGGAAATA
TGAAACTTGATTTTTGGCCGCCTTTGAAATTCCTTCCGCAAACCTTTCCG
GCTTAAACTCAACTGCATCGTAACCCCAAAGTTCAGGATCCTGGTGAACT
AGGATCATTGGCAAGCCAATTTGAACCCCAGCGGGTAATGTCAAGTTTCC
GAGTTTCGTTTCCTTAAGAACAGATCGACTAAGCACAGTTACAGGCGGAT
AAAGCCTGAGTACCTCGTAAAGTATCATCTGGACAATTTTGAGGTGATTC
AATCCATCATAGTTGGGCTTATTATTGCCAAACACTTGCAAAACCTCTTG
CCTTGCACGTTCTTGCCAATCTTGATGCTTGCTCAATAAGACCATCGTCC
AATTAAGCAAAACAGAGGTAGTCTCTTGGCCGGCAAAGTAAAATAGCTTA
CACTCTTCGATCACAT
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