CF832451

Overview
NameCF832451
Unique NameCF832451
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length866
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CF832451 vs. ExPASy Swiss-Prot
Match: C356_FUNHE (Cytochrome P450 3A56 OS=Fundulus heteroclitus GN=cyp3a56 PE=2 SV=1)

HSP 1 Score: 101.679 bits (252), Expect = 5.720e-21
Identity = 58/178 (32.58%), Postives = 96/178 (53.93%), Query Frame = -3
Query:  343 VIEECKLFYFAGQETTSVLLNWTMVLLSKHQDWQERARQEVLQVFGNNKP-NYDGLNHLKIVQMILYEVLRLYPPVTVLSRSVLKETKLGNLTLPAGVQIGLPMILVHQDPELWGYDAVEFKPERFAEGISKAAKNQVS---YFPFALGPRICVGQNFALVEAKMAMAMILQNYSFEL 864
            ++ +  +F FAG ET+S  L +    L+ + +  ++ ++E+   F N  P +Y  L  ++ +  ++ E LRL+P    L R      ++  + +P  + + +P   +H+DPE+W  +   FKPERF    SK  K+ +    Y PF  GPR C+G  FALV  K+A+  ILQ YSF +
Sbjct:  294 ILSQSMIFIFAGYETSSSSLTFLAYNLATNPEVMKKLQEEIDATFPNKAPVHYQPLMEMEYLDCVINESLRLFPIAARLERVAKAAVEINGIVIPKDMVVMIPTWPLHRDPEIWP-EPEAFKPERF----SKKNKDNIDPYIYMPFGSGPRNCIGMRFALVLIKLAVVEILQQYSFSV 466          
BLAST of CF832451 vs. ExPASy Swiss-Prot
Match: CP9E2_BLAGE (Cytochrome P450 9e2 OS=Blattella germanica GN=CYP9E2 PE=2 SV=1)

HSP 1 Score: 101.293 bits (251), Expect = 7.470e-21
Identity = 59/173 (34.10%), Postives = 98/173 (56.65%), Query Frame = -3
Query:  343 LFYFAGQETTSVLLNWTMVLLSKHQDWQERARQEVLQVFGNN--KPNYDGLNHLKIVQMILYEVLRLYPPVTVLSRSVLKETKLG---NLTLPAGVQIGLPMILVHQDPELWGYDAVEFKPERFAEGISKAAKNQVSYFPFALGPRICVGQNFALVEAKMAMAMILQNYSFEL 846
            LF+FAG +T S LL +   LL+ + D Q R + E+ Q    N  K  Y+ ++ +K + M++ E LRLYPP     R  +K  +L    + TL  G  + +P+  +H DP+ +  +  +F PERF++  +K      +Y PF  GPR C+G  FAL+E+K+A+  +L  ++ ++
Sbjct:  330 LFFFAGFDTASTLLCFMSHLLATNPDVQNRLQDEIDQSLEENDGKLTYEAIHSMKYLDMVVSESLRLYPPAIFTDRKCVKNYRLPMEPSYTLEPGDAVWIPIYAIHHDPKYYP-NPEKFDPERFSDE-NKDNIKPFTYLPFGSGPRNCIGNRFALMESKIALVHLLCRFNLKV 500          
BLAST of CF832451 vs. ExPASy Swiss-Prot
Match: CP4F3_HUMAN (Leukotriene-B(4) omega-hydroxylase 2 OS=Homo sapiens GN=CYP4F3 PE=2 SV=2)

HSP 1 Score: 101.293 bits (251), Expect = 7.470e-21
Identity = 62/197 (31.47%), Postives = 100/197 (50.76%), Query Frame = -3
Query:  289 ECKLFYFAGQETTSVLLNWTMVLLSKHQDWQERARQEVLQVFGNNKP---NYDGLNHLKIVQMILYEVLRLYPPVTVLSRSVLKETKL-GNLTLPAGVQIGLPMILVHQDPELWG----YDAVEFKPERFAEGISKAAKNQVSYFPFALGPRICVGQNFALVEAKMAMAMILQNYSFELSPSYVHAPTAVPTLHPEL 855
            E   F F G +TT+  L+W +  L+KH ++QER RQEV ++  + +P    +D L  L  + M + E LRL+PPV  +SR   ++  L     +P G+   + +   H +P +W     YD   F P+   E      ++ +++ PF+ GPR C+GQ FA+ E K+ + + L    F + P +       P   PEL
Sbjct:  321 EADTFMFEGHDTTASGLSWVLYHLAKHPEYQERCRQEVQELLKDREPKEIEWDDLAQLPFLTMCIKESLRLHPPVPAVSRCCTQDIVLPDGRVIPKGIICLISVFGTHHNPAVWPDPEVYDPFRFDPKNIKE------RSPLAFIPFSAGPRNCIGQAFAMAEMKVVLGLTL--LRFRVLPDHTE-----PRRKPEL 504          
BLAST of CF832451 vs. ExPASy Swiss-Prot
Match: CP4CA_MOUSE (Cytochrome P450 4A12A OS=Mus musculus GN=Cyp4a12a PE=2 SV=1)

HSP 1 Score: 101.293 bits (251), Expect = 7.470e-21
Identity = 65/203 (32.02%), Postives = 106/203 (52.22%), Query Frame = -3
Query:  259 ECKLFYFAGQETTSVLLNWTMVLLSKHQDWQERARQEVLQVFGNNKP-NYDGLNHLKIVQMILYEVLRLYPPVTVLSRSVLKETKLGN-LTLPAGVQIGLPMILVHQDPELWGYDAVEFKPERFAEGISKAAKNQVSYFPFALGPRICVGQNFALVEAKMAMAMILQNYSFELSPSYVHAPTAVP--TLHPELGTQLILRKLR 855
            E   F F G +TT+  ++W    L+ + + Q+R R+E+  + G+     ++ L+ +    M + E LR+YPPV  +SR +       +  +LP G+ + L    +H +P +W    V F P RFA G S+ +    S+ PF+ G R C+G+ FA+ E K+A+A+ L    FEL P     P  +P   L  + G  L L+KL+
Sbjct:  312 EVDTFMFEGHDTTASGISWIFYALATNPEHQQRCRKEIQSLLGDGTSITWNDLDKMPYTTMCIKEALRIYPPVPSVSRELSSPVTFPDGRSLPKGIHVMLSFYGLHHNPTVWPNPEV-FDPSRFAPGSSRHSH---SFLPFSGGARNCIGKQFAMNELKVAVALTL--LRFELLPDPTRVPIPIPRIVLKSKNGIHLHLKKLQ 508          
BLAST of CF832451 vs. ExPASy Swiss-Prot
Match: C4G15_DROME (Cytochrome P450 4g15 OS=Drosophila melanogaster GN=Cyp4g15 PE=2 SV=1)

HSP 1 Score: 101.293 bits (251), Expect = 7.470e-21
Identity = 53/176 (30.11%), Postives = 103/176 (58.52%), Query Frame = -3
Query:  355 EECKLFYFAGQETTSVLLNWTMVLLSKHQDWQERARQEVLQVFGNNK--PNYDGLNHLKIVQMILYEVLRLYPPVTVLSRSVLKETKL--GNLTLPAGVQIGLPMILVHQDPELWG----YDAVEFKPERFAEGISKAAKNQVSYFPFALGPRICVGQNFALVEAKMAMAMILQNY 858
            E+     F G +TT+   ++ + L+  HQD Q+R   E+  +FG+++    +     +K ++  L E LR+YPPV +++R + ++ KL  GN  +P G  + +  +L+H++P+++     +D   F PER      +A ++  ++ PF+ GPR CVG+ +A+++ K+ ++ IL+NY
Sbjct:  371 EQVDTIMFEGHDTTAAGSSFFLSLMGIHQDIQDRVLAELDSIFGDSQRPATFQDTLEMKYLERCLMETLRMYPPVPLIARELQEDLKLNSGNYVIPRGATVTVATVLLHRNPKVYANPNVFDPDNFLPER------QANRHYYAFVPFSAGPRSCVGRKYAMLKLKILLSTILRNY 540          
BLAST of CF832451 vs. ExPASy Swiss-Prot
Match: C330_FUNHE (Cytochrome P450 3A30 OS=Fundulus heteroclitus GN=cyp3a30 PE=2 SV=2)

HSP 1 Score: 101.293 bits (251), Expect = 7.470e-21
Identity = 58/178 (32.58%), Postives = 96/178 (53.93%), Query Frame = -3
Query:  343 VIEECKLFYFAGQETTSVLLNWTMVLLSKHQDWQERARQEVLQVFGNNKP-NYDGLNHLKIVQMILYEVLRLYPPVTVLSRSVLKETKLGNLTLPAGVQIGLPMILVHQDPELWGYDAVEFKPERFAEGISKAAKNQVS---YFPFALGPRICVGQNFALVEAKMAMAMILQNYSFEL 864
            ++ +  +F FAG ET+S  L +    L+ + +  ++ ++E+   F N  P +Y  L  ++ +  ++ E LRL+P    L R      ++  + +P  + + +P   +H+DPE+W  +   FKPERF    SK  K+ +    Y PF  GPR C+G  FALV  K+A+  ILQ YSF +
Sbjct:  294 ILSQSMIFIFAGYETSSSSLTFLAYNLATNPEVMKKLQEEIDATFPNKAPVHYQPLMEMEYLDCVINESLRLFPIAARLERVAKAAVEINGVVIPKDMVVMIPTWPLHRDPEIWP-EPEAFKPERF----SKKNKDNIDPYIYMPFGSGPRNCIGMRFALVLIKLAVVEILQQYSFSV 466          
BLAST of CF832451 vs. ExPASy Swiss-Prot
Match: CP4X1_HUMAN (Cytochrome P450 4X1 OS=Homo sapiens GN=CYP4X1 PE=2 SV=1)

HSP 1 Score: 100.908 bits (250), Expect = 9.757e-21
Identity = 67/206 (32.52%), Postives = 106/206 (51.46%), Query Frame = -3
Query:  262 VIEECKLFYFAGQETTSVLLNWTMVLLSKHQDWQERARQEVLQVFGNNKP-NYDGLNHLKIVQMILYEVLRLYPPVTVLSRSVLKE-TKLGNLTLPAGVQIGLPMILVHQDPELWGYDAVEFKPERFAEGISKAAKNQVSYFPFALGPRICVGQNFALVEAKMAMAMILQNYSFELSPSYVHAPTAVPT---LHPELGTQLILRKL 864
            V  E   F  AG +T +  ++W +  L+ + + QER R+EV  + G+     +D L  +    M + E  RL P V  +SR + K  T     TLPAG+ + L +  +H +P +W    V F P RF++  S   ++  +Y PF+ G R C+GQ FA++E K+ +A+IL ++     P+    P   P    L P+ G  L L+KL
Sbjct:  306 VHSEVSTFLLAGHDTLAASISWILYCLALNPEHQERCREEVRGILGDGSSITWDQLGEMSYTTMCIKETCRLIPAVPSISRDLSKPLTFPDGCTLPAGITVVLSIWGLHHNPAVWKNPKV-FDPLRFSQENSDQ-RHPYAYLPFSAGSRNCIGQEFAMIELKVTIALILLHFRVTPDPT---RPLTFPNHFILKPKNGMYLHLKKL 506          
BLAST of CF832451 vs. ExPASy Swiss-Prot
Match: CP4P3_DROME (Probable cytochrome P450 4p3 OS=Drosophila melanogaster GN=Cyp4p3 PE=2 SV=3)

HSP 1 Score: 100.908 bits (250), Expect = 9.757e-21
Identity = 68/207 (32.85%), Postives = 111/207 (53.62%), Query Frame = -3
Query:  265 VIEECKLFYFAGQETTSVLLNWTMVLLSKHQDWQERARQEVLQVFGN--NKPNYDGLNHLKIVQMILYEVLRLYPPVTVLSRSVLKETKLGN-LTLPAGVQIGLPMILVHQDPELWGYDAVEFKPERFAEGISKAAKNQVSYFPFALGPRICVGQNFALVEAKMAMAMILQNYSF--ELSPSYVHAPTAVPTLHP--ELGTQLILRK 864
            + EE     F G +TTS+ L + ++ +S + + QE+  QE+     +  N  N   LN LK ++  + E +RL+P V  + R   +ET+L N L LP G QI + +  +H++PE W     EF+PERF    S+  ++  +Y PF+ G R C+GQ FA+ E K  M  +L+ +    E+ P  +   T + TL    ++  +L+ RK
Sbjct:  312 ICEEVDTLMFEGYDTTSIGLMFGLMNMSLYPEEQEKCYQEIQANIDDELNILNIGQLNKLKNLEYFIKETMRLFPSVPAMGRETTRETELSNGLILPKGSQIFVHVFDIHRNPEYWD-SPEEFRPERFLPENSQN-RHTYAYIPFSAGQRNCIGQKFAMQEMKTLMVALLKQFQILPEIDPKTIVFQTGL-TLRTKNQIHVKLVRRK 515          
BLAST of CF832451 vs. ExPASy Swiss-Prot
Match: CP4A7_RABIT (Cytochrome P450 4A7 OS=Oryctolagus cuniculus GN=CYP4A7 PE=1 SV=1)

HSP 1 Score: 100.908 bits (250), Expect = 9.757e-21
Identity = 66/200 (33.00%), Postives = 104/200 (52.00%), Query Frame = -3
Query:  262 ECKLFYFAGQETTSVLLNWTMVLLSKHQDWQERARQEVLQVFGNNKP-NYDGLNHLKIVQMILYEVLRLYPPVT-VLSRSVLKETKLGNLTLPAGVQIGLPMILVHQDPELWGYDAVEFKPERFAEGISKAAKNQVSYFPFALGPRICVGQNFALVEAKMAMAMILQNYSFELSPSYVHAPTAVPTLHPELGTQLILRKL 855
            E   F F G +TT+  ++W    L+ H + Q R R+E+  + G+     ++ L+ +    M + E LRLYPPV  V S+     T     +LP G+ I L +  +H +P++W    V F P RFA G   +A++  ++ PF+ G R C+G+ FA+ E K+A+A+ L  +     P+ V  P     L  + G  L LRKL
Sbjct:  315 EVDTFMFEGHDTTASGISWIFYALATHPEHQHRCREEIQGLLGDGASITWEHLDKMPYTTMCIKEALRLYPPVPGVGSKLSSPVTFPDGRSLPKGIIITLSIYGLHHNPKVWPNPEV-FDPSRFAPG---SARHSHAFLPFSGGSRNCIGKQFAMNELKVAVALTLVRFELLPDPTRVPIPITRLVLKSKNGIHLRLRKL 510          
BLAST of CF832451 vs. ExPASy Swiss-Prot
Match: CP4C1_BLADI (Cytochrome P450 4C1 OS=Blaberus discoidalis GN=CYP4C1 PE=2 SV=1)

HSP 1 Score: 100.523 bits (249), Expect = 1.274e-20
Identity = 54/174 (31.03%), Postives = 96/174 (55.17%), Query Frame = -3
Query:  355 EECKLFYFAGQETTSVLLNWTMVLLSKHQDWQERARQEVLQVF--GNNKPNYDGLNHLKIVQMILYEVLRLYPPVTVLSRSVLKETKLGNLTLPAGVQIGLPMILVHQDPELW----GYDAVEFKPERFAEGISKAAKNQVSYFPFALGPRICVGQNFALVEAKMAMAMILQNY 858
            EE   F F G +TTS  + W + LL  H + Q++  +E+  +F   +       L  +K ++ ++ E LRL+P V  + R + ++TK+G+  +PAG  + L +  VH++ + +     ++   F PER A+      ++  +Y PF+ GPR C+GQ FA +E K  ++ IL+N+
Sbjct:  306 EEVDTFMFEGHDTTSAGICWALFLLGSHPEIQDKVYEELDHIFQGSDRSTTMRDLADMKYLERVIKESLRLFPSVPFIGRVLKEDTKIGDYLVPAGCMMNLQIYHVHRNQDQYPNPEAFNPDNFLPERVAK------RHPYAYVPFSAGPRNCIGQKFATLEEKTVLSSILRNF 473          
The following BLAST results are available for this feature:
BLAST of CF832451 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 457
Match NameE-valueIdentityDescription
C356_FUNHE5.720e-2132.58Cytochrome P450 3A56 OS=Fundulus heteroclitus GN=c... [more]
CP9E2_BLAGE7.470e-2134.10Cytochrome P450 9e2 OS=Blattella germanica GN=CYP9... [more]
CP4F3_HUMAN7.470e-2131.47Leukotriene-B(4) omega-hydroxylase 2 OS=Homo sapie... [more]
CP4CA_MOUSE7.470e-2132.02Cytochrome P450 4A12A OS=Mus musculus GN=Cyp4a12a ... [more]
C4G15_DROME7.470e-2130.11Cytochrome P450 4g15 OS=Drosophila melanogaster GN... [more]
C330_FUNHE7.470e-2132.58Cytochrome P450 3A30 OS=Fundulus heteroclitus GN=c... [more]
CP4X1_HUMAN9.757e-2132.52Cytochrome P450 4X1 OS=Homo sapiens GN=CYP4X1 PE=2... [more]
CP4P3_DROME9.757e-2132.85Probable cytochrome P450 4p3 OS=Drosophila melanog... [more]
CP4A7_RABIT9.757e-2133.00Cytochrome P450 4A7 OS=Oryctolagus cuniculus GN=CY... [more]
CP4C1_BLADI1.274e-2031.03Cytochrome P450 4C1 OS=Blaberus discoidalis GN=CYP... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS02_01O03_f Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa01O03, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF832451 ID=CF832451; Name=CF832451; organism=Citrus sinensis; type=EST; length=866bp
TTTTTTTTTTTTTTACCCAGCATTAATGCTCTAATTCTAATCATGGTATT
ATAAAATTTTCCAATGTGGGGAAACATCTTTACTCTAAATAAAACTAGAG
TTGTGTTGAGCTCAATTTAAAAATCTAAACAGCTCTTGTCTAAGCTTTGA
ATCTCAATACAAAATTAAATACATTAAACTTCATTATAAAAGAGTCTACA
TTGGCATCATGCTAATCTTATTGACAATATATAGTTGCCGGTATTAATTA
TTTTTGCACCTTAGTTTCCGTAGAATCAATTGGGTACCCAGTTCCGGATG
AAGGGTTGGAACCGCCGTTGGAGCATGGACATAGGATGGAGACAACTCGA
ATGAGTAGTTTTGAAGAATCATTGCCATGGCCATTTTTGCTTCTACTAGA
GCAAAATTTTGTCCAACACATATCCTAGGACCCAATGCGAATGGGAAATA
TGAAACTTGATTTTTGGCCGCCTTTGAAATTCCTTCCGCAAACCTTTCCG
GCTTAAACTCAACTGCATCGTAACCCCAAAGTTCAGGATCCTGGTGAACT
AGGATCATTGGCAAGCCAATTTGAACCCCAGCGGGTAATGTCAAGTTTCC
GAGTTTCGTTTCCTTAAGAACAGATCGACTAAGCACAGTTACAGGCGGAT
AAAGCCTGAGTACCTCGTAAAGTATCATCTGGACAATTTTGAGGTGATTC
AATCCATCATAGTTGGGCTTATTATTGCCAAACACTTGCAAAACCTCTTG
CCTTGCACGTTCTTGCCAATCTTGATGCTTGCTCAATAAGACCATCGTCC
AATTAAGCAAAACAGAGGTAGTCTCTTGGCCGGCAAAGTAAAATAGCTTA
CACTCTTCGATCACAT
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