CF832474

Overview
NameCF832474
Unique NameCF832474
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length751
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig1234 contig Csv1_Contig1234:172..922. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CF832474 vs. ExPASy Swiss-Prot
Match: METK3_ARATH (S-adenosylmethionine synthetase 3 OS=Arabidopsis thaliana GN=METK3 PE=1 SV=1)

HSP 1 Score: 427.943 bits (1099), Expect = 2.778e-119
Identity = 205/225 (91.11%), Postives = 212/225 (94.22%), Query Frame = 2 
Query:   77 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETPEFMPLSHVLATKLGARLTEVRKNSTCSWLRPDGKTQVTVEYYNDNGAMVPVRVHTVLISTQHDETVTNDEIAADLKEHVIKPVIPEKYLD 751
            METFLFTSESVNEGHPDKLCDQISDA+LDACLEQDPESKVACETCTKTNMVMVFGEITT AKVDYEKIVR TCR IGF+S DVGLDAD C VLVNIEQQSPDIAQGVHGH TK+PEDIGAGDQGHMFGYATDETPE MPL+HVLATKLGA+LTEVRKN TC WLRPDGKTQVTVEY ND GAM+P+RVHTVLISTQHDETVTNDEIAADLKEHVIKPVIP KYLD
Sbjct:    1 METFLFTSESVNEGHPDKLCDQISDAILDACLEQDPESKVACETCTKTNMVMVFGEITTAAKVDYEKIVRSTCREIGFISADVGLDADKCNVLVNIEQQSPDIAQGVHGHLTKKPEDIGAGDQGHMFGYATDETPELMPLTHVLATKLGAKLTEVRKNKTCPWLRPDGKTQVTVEYKNDGGAMIPIRVHTVLISTQHDETVTNDEIAADLKEHVIKPVIPAKYLD 225
[Close]
BLAST of CF832474 vs. ExPASy Swiss-Prot
Match: METK2_PETHY (S-adenosylmethionine synthetase 2 OS=Petunia hybrida GN=SAM2 PE=2 SV=1)

HSP 1 Score: 426.787 bits (1096), Expect = 6.190e-119
Identity = 201/225 (89.33%), Postives = 214/225 (95.11%), Query Frame = 2 
Query:   77 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETPEFMPLSHVLATKLGARLTEVRKNSTCSWLRPDGKTQVTVEYYNDNGAMVPVRVHTVLISTQHDETVTNDEIAADLKEHVIKPVIPEKYLD 751
            METFLFTSESVNEGHPDKLCDQ+SDA+LDACLEQDPESKVACETCTKT+MVMVFGEITTKA VDYEKIVRDTCR IGF S DVGLDADHCKVLVNIEQQSPDIAQGVHGH TK+PE+IGAGDQGHMFGYATDETPE MPL+HVLATKLGA+LTEVRKN TC WLRPDGKTQVTVEY NDNGAM+P+RVHT+LISTQHDETVTND+IA DLKEHVIKPV+P +YLD
Sbjct:    1 METFLFTSESVNEGHPDKLCDQVSDAILDACLEQDPESKVACETCTKTSMVMVFGEITTKATVDYEKIVRDTCRGIGFTSADVGLDADHCKVLVNIEQQSPDIAQGVHGHLTKKPEEIGAGDQGHMFGYATDETPELMPLTHVLATKLGAKLTEVRKNKTCPWLRPDGKTQVTVEYRNDNGAMIPLRVHTILISTQHDETVTNDQIAQDLKEHVIKPVVPAEYLD 225
[Close]
BLAST of CF832474 vs. ExPASy Swiss-Prot
Match: METK2_ATRNU (S-adenosylmethionine synthetase 2 OS=Atriplex nummularia GN=SAMS2 PE=1 SV=1)

HSP 1 Score: 426.787 bits (1096), Expect = 6.190e-119
Identity = 205/225 (91.11%), Postives = 215/225 (95.56%), Query Frame = 2 
Query:   77 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETPEFMPLSHVLATKLGARLTEVRKNSTCSWLRPDGKTQVTVEYYNDNGAMVPVRVHTVLISTQHDETVTNDEIAADLKEHVIKPVIPEKYLD 751
            ++TFLFTSESVNEGHPDKLCDQISDAVLDACL QDPESKVACETCTKTN+VMVFGEITTKA VDYEKIVR TCR IGFVS DVGLDAD+CKVLV IEQQSPDIAQGVHGH ++RPE+IGAGDQGHMFGYA+DETPE MPLSHVLATKLGARLTEVRKN TC WLRPDGKTQVTVEYYN+NGAMVP+RVHTVLISTQHDETVTNDEIAADLKEHVIKPVIPEKYLD
Sbjct:    5 VDTFLFTSESVNEGHPDKLCDQISDAVLDACLAQDPESKVACETCTKTNLVMVFGEITTKADVDYEKIVRQTCRDIGFVSADVGLDADNCKVLVYIEQQSPDIAQGVHGHLSRRPEEIGAGDQGHMFGYASDETPELMPLSHVLATKLGARLTEVRKNGTCPWLRPDGKTQVTVEYYNENGAMVPIRVHTVLISTQHDETVTNDEIAADLKEHVIKPVIPEKYLD 229
[Close]
BLAST of CF832474 vs. ExPASy Swiss-Prot
Match: METK1_PETHY (S-adenosylmethionine synthetase 1 OS=Petunia hybrida GN=SAM1 PE=2 SV=1)

HSP 1 Score: 426.402 bits (1095), Expect = 8.084e-119
Identity = 201/225 (89.33%), Postives = 213/225 (94.67%), Query Frame = 2 
Query:   77 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETPEFMPLSHVLATKLGARLTEVRKNSTCSWLRPDGKTQVTVEYYNDNGAMVPVRVHTVLISTQHDETVTNDEIAADLKEHVIKPVIPEKYLD 751
            METFLFTSESVNEGHPDKLCDQ+SDA+LDACLEQDPESKVACETCTKTNMVMVFGEITTKA VDYEKIVRDTCR IGF S DVGLDADHCKVLVNIEQQSPDIAQGVHGH TK+PE+IGAGDQGHMFGYATDETPE MPL+HV ATKLGA+LTEVRKN TC WLRPDGKTQVTVEY NDNGAM+P+RVHT+LISTQHDETVTND+IA DLKEHVIKPV+P +YLD
Sbjct:    1 METFLFTSESVNEGHPDKLCDQVSDAILDACLEQDPESKVACETCTKTNMVMVFGEITTKATVDYEKIVRDTCRGIGFTSADVGLDADHCKVLVNIEQQSPDIAQGVHGHLTKKPEEIGAGDQGHMFGYATDETPELMPLTHVWATKLGAKLTEVRKNKTCPWLRPDGKTQVTVEYRNDNGAMIPLRVHTILISTQHDETVTNDQIAQDLKEHVIKPVVPAEYLD 225
[Close]
BLAST of CF832474 vs. ExPASy Swiss-Prot
Match: METK1_TOBAC (S-adenosylmethionine synthetase 1 OS=Nicotiana tabacum GN=SAMS1 PE=2 SV=1)

HSP 1 Score: 426.017 bits (1094), Expect = 1.056e-118
Identity = 203/225 (90.22%), Postives = 213/225 (94.67%), Query Frame = 2 
Query:   77 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETPEFMPLSHVLATKLGARLTEVRKNSTCSWLRPDGKTQVTVEYYNDNGAMVPVRVHTVLISTQHDETVTNDEIAADLKEHVIKPVIPEKYLD 751
            METFLFTSESVNEGHPDKLCDQ+SDA+LDACLEQDPESKVACETCTKTNMVMVFGEITTKA VDYEKIVRDTCR IGF S DVGLDAD+CKVLVNIEQQSPDIAQGVHGH TK+PE+IGAGDQGHMFGYATDETPE MPL+HV ATKLGA+LTEVRKN TC WLRPDGKTQVTVEY NDNGAMVP+RVHTVLISTQHDETVTND+IA DLKEHVIKPVIP +YLD
Sbjct:    1 METFLFTSESVNEGHPDKLCDQVSDAILDACLEQDPESKVACETCTKTNMVMVFGEITTKATVDYEKIVRDTCRGIGFTSADVGLDADNCKVLVNIEQQSPDIAQGVHGHLTKKPEEIGAGDQGHMFGYATDETPELMPLTHVWATKLGAKLTEVRKNKTCPWLRPDGKTQVTVEYKNDNGAMVPIRVHTVLISTQHDETVTNDQIAQDLKEHVIKPVIPSQYLD 225
[Close]
BLAST of CF832474 vs. ExPASy Swiss-Prot
Match: METK1_PICSI (S-adenosylmethionine synthetase 1 OS=Picea sitchensis GN=METK1 PE=2 SV=1)

HSP 1 Score: 426.017 bits (1094), Expect = 1.056e-118
Identity = 202/225 (89.78%), Postives = 215/225 (95.56%), Query Frame = 2 
Query:   77 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETPEFMPLSHVLATKLGARLTEVRKNSTCSWLRPDGKTQVTVEYYNDNGAMVPVRVHTVLISTQHDETVTNDEIAADLKEHVIKPVIPEKYLD 751
            M+TFLFTSESVNEGHPDKLCDQISDA+LDACLEQDPESKVACETCTKTNMVM+FGEITTKA +DYEKIVRDTCR IGFVS DVGLDAD+CKVLVNIEQQSPDIAQGVHGH TK+PE+IGAGDQGHMFGYATDETPE MPL+HVLATKLGARLTEVRK+ TC WLRPDGKTQVTVEY N+ GAMVP+RVHTVLISTQHDETVTNDEIAADLKEHVIKPV+P +YLD
Sbjct:    1 MDTFLFTSESVNEGHPDKLCDQISDAILDACLEQDPESKVACETCTKTNMVMIFGEITTKANIDYEKIVRDTCRGIGFVSADVGLDADNCKVLVNIEQQSPDIAQGVHGHLTKKPEEIGAGDQGHMFGYATDETPELMPLTHVLATKLGARLTEVRKDGTCPWLRPDGKTQVTVEYKNEGGAMVPLRVHTVLISTQHDETVTNDEIAADLKEHVIKPVVPSQYLD 225
[Close]
BLAST of CF832474 vs. ExPASy Swiss-Prot
Match: METK4_BRAJU (S-adenosylmethionine synthetase 4 OS=Brassica juncea GN=MSAMS4 PE=2 SV=1)

HSP 1 Score: 424.861 bits (1091), Expect = 2.352e-118
Identity = 202/225 (89.78%), Postives = 211/225 (93.78%), Query Frame = 2 
Query:   77 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETPEFMPLSHVLATKLGARLTEVRKNSTCSWLRPDGKTQVTVEYYNDNGAMVPVRVHTVLISTQHDETVTNDEIAADLKEHVIKPVIPEKYLD 751
            METFLFTSESVNEGHPDKLCDQ+SDA+LDACLEQDPESKVACETCTKTNMVMVFGEITT AKVDYEKIVR TCR IGF+S DVGLDAD C VLVNIEQQSPDIAQGVHGH TK+PEDIGAGDQGHMFGYATDETPE MPL+HVLATKLGA+LTEVRKN TC WLRPDGKTQVTVEY N+ GAM+P+RVHTVLISTQHDETVTNDEIA DLKEHVIKPVIP KYLD
Sbjct:    1 METFLFTSESVNEGHPDKLCDQVSDAILDACLEQDPESKVACETCTKTNMVMVFGEITTSAKVDYEKIVRSTCREIGFISADVGLDADKCNVLVNIEQQSPDIAQGVHGHLTKKPEDIGAGDQGHMFGYATDETPELMPLTHVLATKLGAKLTEVRKNKTCPWLRPDGKTQVTVEYKNEGGAMIPIRVHTVLISTQHDETVTNDEIAVDLKEHVIKPVIPAKYLD 225
[Close]
BLAST of CF832474 vs. ExPASy Swiss-Prot
Match: METK2_ACTCH (S-adenosylmethionine synthetase 2 OS=Actinidia chinensis GN=SAM2 PE=2 SV=1)

HSP 1 Score: 422.935 bits (1086), Expect = 8.938e-118
Identity = 200/225 (88.89%), Postives = 215/225 (95.56%), Query Frame = 2 
Query:   77 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETPEFMPLSHVLATKLGARLTEVRKNSTCSWLRPDGKTQVTVEYYNDNGAMVPVRVHTVLISTQHDETVTNDEIAADLKEHVIKPVIPEKYLD 751
            ME+FLFTSESVNEGHPDKLCDQ+SDA+LDACLEQDPESKVACETCTKTNMVMVFGEITTKAKV+YEKIVRDTCR IGF S DVGLDADHCKVLVNIEQQSPDIAQGVHGH +K+PE+IGAGDQGHMFGYATDETPE MPL+HVLATKLGA+LTEVRKN+TC WLRPDGKTQVTVEY N+ GAMVPVRVHTVLISTQHDETVTN++IA DLKEHVIKPVIP +Y+D
Sbjct:    1 MESFLFTSESVNEGHPDKLCDQVSDAILDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVNYEKIVRDTCRGIGFTSPDVGLDADHCKVLVNIEQQSPDIAQGVHGHLSKKPEEIGAGDQGHMFGYATDETPELMPLTHVLATKLGAKLTEVRKNNTCPWLRPDGKTQVTVEYRNEGGAMVPVRVHTVLISTQHDETVTNEQIAKDLKEHVIKPVIPAQYMD 225
[Close]
BLAST of CF832474 vs. ExPASy Swiss-Prot
Match: METK1_VITVI (S-adenosylmethionine synthetase 1 OS=Vitis vinifera GN=METK1 PE=3 SV=1)

HSP 1 Score: 422.935 bits (1086), Expect = 8.938e-118
Identity = 200/225 (88.89%), Postives = 215/225 (95.56%), Query Frame = 2 
Query:   77 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETPEFMPLSHVLATKLGARLTEVRKNSTCSWLRPDGKTQVTVEYYNDNGAMVPVRVHTVLISTQHDETVTNDEIAADLKEHVIKPVIPEKYLD 751
            M+TFLFTSESVNEGHPDKLCDQ+SDA+LDACLEQDPESKVACETCTKTNMVMVFGEITTKAKV+YEKIVRDTCR IGFVS DVGLDAD+CKVLVNIEQQSPDIAQGVHGH TK+PE+IGAGDQGHMFGYATDETPE MPL+HVLATKLGA+LTEVRKN TC WLRPDGKTQVTVEY N+ GAMVP+RVHTVL+STQHDETVTN++IA DLKEHVIKPVIP +YLD
Sbjct:    1 MDTFLFTSESVNEGHPDKLCDQVSDAILDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVNYEKIVRDTCRGIGFVSADVGLDADNCKVLVNIEQQSPDIAQGVHGHLTKKPEEIGAGDQGHMFGYATDETPELMPLTHVLATKLGAKLTEVRKNGTCPWLRPDGKTQVTVEYRNEGGAMVPIRVHTVLVSTQHDETVTNEQIAKDLKEHVIKPVIPPQYLD 225
[Close]
BLAST of CF832474 vs. ExPASy Swiss-Prot
Match: METK_PINBN (S-adenosylmethionine synthetase OS=Pinus banksiana GN=METK PE=2 SV=1)

HSP 1 Score: 422.55 bits (1085), Expect = 1.167e-117
Identity = 205/225 (91.11%), Postives = 210/225 (93.33%), Query Frame = 2 
Query:   77 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETPEFMPLSHVLATKLGARLTEVRKNSTCSWLRPDGKTQVTVEYYNDNGAMVPVRVHTVLISTQHDETVTNDEIAADLKEHVIKPVIPEKYLD 751
            METFLFTSESVNEGHPDKLCDQISDAVLDACL QDP+SKVACETCTKTNMVMVFGEITTKA VDYE+IVR TCR IGF+SDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPE+IGAGDQGHMFGYATDET E MP  HVLATKLGA+LTEVRKN TC WLRP GKTQVTVEY NDNGAMVP RVHTVLISTQHDETV NDEIA DLKEHVIKPVIPEKYLD
Sbjct:    1 METFLFTSESVNEGHPDKLCDQISDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKADVDYEQIVRKTCREIGFISDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEEIGAGDQGHMFGYATDETLELMPKCHVLATKLGAKLTEVRKNGTCPWLRPHGKTQVTVEYRNDNGAMVPERVHTVLISTQHDETVPNDEIAPDLKEHVIKPVIPEKYLD 225
The following BLAST results are available for this feature:
BLAST of CF832474 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
METK3_ARATH2.778e-11991.11S-adenosylmethionine synthetase 3 OS=Arabidopsis t... [more]
METK2_PETHY6.190e-11989.33S-adenosylmethionine synthetase 2 OS=Petunia hybri... [more]
METK2_ATRNU6.190e-11991.11S-adenosylmethionine synthetase 2 OS=Atriplex numm... [more]
METK1_PETHY8.084e-11989.33S-adenosylmethionine synthetase 1 OS=Petunia hybri... [more]
METK1_TOBAC1.056e-11890.22S-adenosylmethionine synthetase 1 OS=Nicotiana tab... [more]
METK1_PICSI1.056e-11889.78S-adenosylmethionine synthetase 1 OS=Picea sitchen... [more]
METK4_BRAJU2.352e-11889.78S-adenosylmethionine synthetase 4 OS=Brassica junc... [more]
METK2_ACTCH8.938e-11888.89S-adenosylmethionine synthetase 2 OS=Actinidia chi... [more]
METK1_VITVI8.938e-11888.89S-adenosylmethionine synthetase 1 OS=Vitis vinifer... [more]
METK_PINBN1.167e-11791.11S-adenosylmethionine synthetase OS=Pinus banksiana... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS02_01O16_r Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa01O16, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF832474 ID=CF832474; Name=CF832474; organism=Citrus sinensis; type=EST; length=751bp
CTTTGCTATTGCGGTCTGGTCTTAAGTCTGAATCTTAGATCTGCGCCGAA
ACACTTGCCAGCCACAGCTTGATAAGATGGAGACCTTTCTATTCACCTCT
GAATCTGTGAACGAGGGTCACCCCGACAAGTTGTGCGACCAGATCTCTGA
TGCGGTGCTTGATGCCTGCCTTGAGCAGGATCCTGAGAGCAAGGTTGCCT
GTGAGACATGCACCAAGACCAACATGGTCATGGTCTTTGGAGAGATTACC
ACCAAGGCCAAGGTAGACTATGAGAAGATTGTTCGCGACACTTGCCGTTC
CATTGGATTTGTTTCTGATGATGTGGGTCTTGATGCCGATCACTGCAAAG
TCCTGGTCAATATTGAGCAACAAAGCCCTGATATTGCCCAGGGTGTTCAT
GGCCACTTTACAAAGCGCCCTGAGGACATTGGTGCTGGTGACCAGGGCCA
TATGTTTGGTTATGCTACTGATGAGACCCCTGAATTTATGCCTTTGAGCC
ATGTCCTTGCCACCAAACTTGGTGCTCGTCTCACGGAGGTTAGGAAGAAT
AGTACCTGCTCTTGGCTAAGGCCCGATGGGAAGACCCAGGTCACTGTTGA
GTACTACAATGACAATGGTGCCATGGTCCCAGTCCGTGTCCACACGGTCC
TCATTTCAACCCAGCATGATGAGACTGTCACAAATGATGAAATTGCTGCT
GACCTTAAGGAGCACGTCATTAAGCCTGTCATCCCCGAGAAGTACCTTGA
T
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