CF832535

Overview
NameCF832535
Unique NameCF832535
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length651
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CF832535 vs. ExPASy Swiss-Prot
Match: RRF_CLOBA (Ribosome-recycling factor OS=Clostridium botulinum (strain Alaska E43 / Type E3) GN=frr PE=3 SV=1)

HSP 1 Score: 150.214 bits (378), Expect = 8.623e-36
Identity = 75/143 (52.45%), Postives = 101/143 (70.63%), Query Frame = -2
Query:  222 SNPAMLDKIEVEYYGSPVSLKSIAQINTPDSSSLLIQPYDKSSLKSIEKAIVSSDLGMTPNNDGEVIRLTLPQLTSERRKELSKVVAKQAEEGKVAVRNIRRDALKAYEKLQKEKKLSEDNVKDLSSDLQKLTDEYMKKIDSI 650
            +NP+MLDKI+++YYGS   L   A I++P+   L+I P++K  LK IEKAI+ SDLG+ P+NDG +IRL +P+LT E RK+L K V K  EE KVA+R+IRRDA    + L+K+  LSED VK    D+QK TD  +K+ID I
Sbjct:   32 ANPSMLDKIQIDYYGSMCPLSQAANISSPEPRVLMITPWEKQLLKEIEKAILKSDLGLNPSNDGSIIRLVIPELTEETRKDLVKKVKKTGEESKVAIRSIRRDANDKIKALKKDGDLSEDQVKKGEDDVQKKTDAIIKEIDKI 174          
BLAST of CF832535 vs. ExPASy Swiss-Prot
Match: RRF_STRSV (Ribosome-recycling factor OS=Streptococcus sanguinis (strain SK36) GN=frr PE=3 SV=1)

HSP 1 Score: 149.828 bits (377), Expect = 1.126e-35
Identity = 72/143 (50.35%), Postives = 103/143 (72.03%), Query Frame = -2
Query:  222 SNPAMLDKIEVEYYGSPVSLKSIAQINTPDSSSLLIQPYDKSSLKSIEKAIVSSDLGMTPNNDGEVIRLTLPQLTSERRKELSKVVAKQAEEGKVAVRNIRRDALKAYEKLQKEKKLSEDNVKDLSSDLQKLTDEYMKKIDSI 650
            +N ++LD+I VEYYG    L  IA I  P++  LL+ P+DKSS+K IE+A+ +SDLG+TP +DG VIRL +P LT E R++L+K V K  E  KVA+RNIRRDA+   +K +K K+++ED +K L  D+QK+TD+ +K ID +
Sbjct:   32 ANASLLDRIHVEYYGVETPLNQIASITIPEARVLLVTPFDKSSIKDIERALNASDLGITPASDGSVIRLVIPALTEETRRDLAKEVKKVGENAKVAIRNIRRDAMDEAKKQEKAKEITEDELKTLEKDIQKVTDDAVKHIDEM 174          
BLAST of CF832535 vs. ExPASy Swiss-Prot
Match: RRF_LACJO (Ribosome-recycling factor OS=Lactobacillus johnsonii GN=frr PE=3 SV=1)

HSP 1 Score: 149.828 bits (377), Expect = 1.126e-35
Identity = 69/146 (47.26%), Postives = 106/146 (72.60%), Query Frame = -2
Query:  213 SNPAMLDKIEVEYYGSPVSLKSIAQINTPDSSSLLIQPYDKSSLKSIEKAIVSSDLGMTPNNDGEVIRLTLPQLTSERRKELSKVVAKQAEEGKVAVRNIRRDALKAYEKLQKEKKLSEDNVKDLSSDLQKLTDEYMKKIDSIYKQ 650
            +N ++LD I+V YYG P  L  ++ ++ P++  L++ PYDKS+L  IE AI++SDLG+TP NDG VIR+ +PQLT ERR+E++K V K AE+GK+AVRN+RRDA+   ++ +K+  ++ED  + L   +QK+TD+  K+ID +  Q
Sbjct:   32 ANASLLDGIKVNYYGVPTPLTQMSSVSIPEARVLMVTPYDKSTLDDIEHAILASDLGITPANDGTVIRIVIPQLTGERRQEIAKQVGKLAEKGKIAVRNVRRDAMDTLKRQEKDGDITEDEQRSLEKQVQKVTDDATKEIDKLADQ 177          
BLAST of CF832535 vs. ExPASy Swiss-Prot
Match: RRF_EXIS2 (Ribosome-recycling factor OS=Exiguobacterium sibiricum (strain DSM 17290 / JCM 13490 / 255-15) GN=frr PE=3 SV=1)

HSP 1 Score: 149.828 bits (377), Expect = 1.126e-35
Identity = 70/141 (49.65%), Postives = 105/141 (74.47%), Query Frame = -2
Query:  228 SNPAMLDKIEVEYYGSPVSLKSIAQINTPDSSSLLIQPYDKSSLKSIEKAIVSSDLGMTPNNDGEVIRLTLPQLTSERRKELSKVVAKQAEEGKVAVRNIRRDALKAYEKLQKEKKLSEDNVKDLSSDLQKLTDEYMKKID 650
            +N ++LD ++V+YYGSP  L  +A +NTP++  +LI P+DKS +  IEKAI  +DLG+ P++DG VIRL +P LT +RRKEL K+V K  EEGKVA+RNIRRD  +  +K +K+  L+ED+++  + D+Q LTD+++K +D
Sbjct:   32 ANVSILDPVQVDYYGSPTPLNQVANVNTPEARLILITPWDKSMVTEIEKAIQRADLGLAPSSDGTVIRLAIPPLTEDRRKELVKLVKKYTEEGKVALRNIRRDTNEQLKKQEKDGVLTEDDLRGYTEDVQTLTDKFVKVLD 172          
BLAST of CF832535 vs. ExPASy Swiss-Prot
Match: RRF_LACGA (Ribosome-recycling factor OS=Lactobacillus gasseri (strain ATCC 33323 / DSM 20243) GN=frr PE=3 SV=1)

HSP 1 Score: 149.443 bits (376), Expect = 1.471e-35
Identity = 69/146 (47.26%), Postives = 106/146 (72.60%), Query Frame = -2
Query:  213 SNPAMLDKIEVEYYGSPVSLKSIAQINTPDSSSLLIQPYDKSSLKSIEKAIVSSDLGMTPNNDGEVIRLTLPQLTSERRKELSKVVAKQAEEGKVAVRNIRRDALKAYEKLQKEKKLSEDNVKDLSSDLQKLTDEYMKKIDSIYKQ 650
            +N ++L+ I+V+YYG P  L  ++ ++ P++  L++ PYDKSSL  IE AI++SDLG+TP NDG VIR+ +PQLT ERR+E++K V K AE+GK+AVRN+RRDA+   ++ +K   ++ED  + L   +QK+TD+  K+ID +  Q
Sbjct:   32 ANASLLEGIKVDYYGVPTPLTQMSSVSIPEARVLMVTPYDKSSLDDIEHAILASDLGITPANDGTVIRIVIPQLTGERRQEIAKQVGKLAEKGKIAVRNVRRDAMDTLKRQEKNGDITEDEQRSLEKQVQKVTDDATKEIDKLADQ 177          
BLAST of CF832535 vs. ExPASy Swiss-Prot
Match: RRF_LACCB (Ribosome-recycling factor OS=Lactobacillus casei (strain BL23) GN=frr PE=3 SV=1)

HSP 1 Score: 149.443 bits (376), Expect = 1.471e-35
Identity = 71/143 (49.65%), Postives = 103/143 (72.03%), Query Frame = -2
Query:  222 SNPAMLDKIEVEYYGSPVSLKSIAQINTPDSSSLLIQPYDKSSLKSIEKAIVSSDLGMTPNNDGEVIRLTLPQLTSERRKELSKVVAKQAEEGKVAVRNIRRDALKAYEKLQKEKKLSEDNVKDLSSDLQKLTDEYMKKIDSI 650
            +N ++L++I VEYYG+P  L  +A I  P+   L + PYDKSSLK+IE A+ +SDLG+ P NDG+VIRL +PQLT ERRKE++K V K +E  K+A+RNIRR+ L   ++ +K+  ++ED++  L  D+QK TD+  K+ID I
Sbjct:   32 ANASLLNQITVEYYGAPTPLNQMAAITIPEPRVLQVSPYDKSSLKNIETALNASDLGINPANDGDVIRLVIPQLTGERRKEIAKEVGKYSESAKIAIRNIRREGLDKLKRQEKDGDITEDDLHRLEKDMQKATDDATKRIDEI 174          
BLAST of CF832535 vs. ExPASy Swiss-Prot
Match: RRF_LACC3 (Ribosome-recycling factor OS=Lactobacillus casei (strain ATCC 334) GN=frr PE=3 SV=1)

HSP 1 Score: 149.443 bits (376), Expect = 1.471e-35
Identity = 71/143 (49.65%), Postives = 103/143 (72.03%), Query Frame = -2
Query:  222 SNPAMLDKIEVEYYGSPVSLKSIAQINTPDSSSLLIQPYDKSSLKSIEKAIVSSDLGMTPNNDGEVIRLTLPQLTSERRKELSKVVAKQAEEGKVAVRNIRRDALKAYEKLQKEKKLSEDNVKDLSSDLQKLTDEYMKKIDSI 650
            +N ++L++I VEYYG+P  L  +A I  P+   L + PYDKSSLK+IE A+ +SDLG+ P NDG+VIRL +PQLT ERRKE++K V K +E  K+A+RNIRR+ L   ++ +K+  ++ED++  L  D+QK TD+  K+ID I
Sbjct:   32 ANASLLNQITVEYYGAPTPLNQMAAITIPEPRVLQVSPYDKSSLKNIETALNASDLGINPANDGDVIRLVIPQLTGERRKEIAKEVGKYSESAKIAIRNIRREGLDKLKRQEKDGDITEDDLHRLEKDMQKATDDATKRIDEI 174          
BLAST of CF832535 vs. ExPASy Swiss-Prot
Match: RRF_CLOBB (Ribosome-recycling factor OS=Clostridium botulinum (strain Eklund 17B / Type B) GN=frr PE=3 SV=1)

HSP 1 Score: 149.443 bits (376), Expect = 1.471e-35
Identity = 75/143 (52.45%), Postives = 100/143 (69.93%), Query Frame = -2
Query:  222 SNPAMLDKIEVEYYGSPVSLKSIAQINTPDSSSLLIQPYDKSSLKSIEKAIVSSDLGMTPNNDGEVIRLTLPQLTSERRKELSKVVAKQAEEGKVAVRNIRRDALKAYEKLQKEKKLSEDNVKDLSSDLQKLTDEYMKKIDSI 650
            +NP+MLDKI+++YYGS   L   A I++P+   L+I P++K  LK IEKAI+ SDLG+ P+NDG +IRL +P+LT E RK+L K V K  EE KVA+R+IRRDA    + L+K+  LSED VK    D+QK TD  +K ID I
Sbjct:   32 ANPSMLDKIQIDYYGSMCPLSQAANISSPEPRVLMITPWEKQLLKEIEKAILKSDLGLNPSNDGSIIRLVIPELTEETRKDLVKKVKKTGEESKVAIRSIRRDANDKIKALKKDGDLSEDQVKKGEDDVQKKTDAIIKDIDKI 174          
BLAST of CF832535 vs. ExPASy Swiss-Prot
Match: RRF_CLOPS (Ribosome-recycling factor OS=Clostridium perfringens (strain SM101 / Type A) GN=frr PE=3 SV=1)

HSP 1 Score: 148.673 bits (374), Expect = 2.509e-35
Identity = 71/143 (49.65%), Postives = 103/143 (72.03%), Query Frame = -2
Query:  222 SNPAMLDKIEVEYYGSPVSLKSIAQINTPDSSSLLIQPYDKSSLKSIEKAIVSSDLGMTPNNDGEVIRLTLPQLTSERRKELSKVVAKQAEEGKVAVRNIRRDALKAYEKLQKEKKLSEDNVKDLSSDLQKLTDEYMKKIDSI 650
            +NP MLD+I+VEYYGS   L  +A +++P+   L+I P++K  LK IEKAI+ SDLG+ PNNDG +IRL +P+LT E RK L K V K  E+ KVA+R+IR+DA    +  +KE  ++ED +K    D+QK+TD+++K+ID+I
Sbjct:   32 ANPTMLDRIQVEYYGSMCPLNQVANVSSPEPRLLVITPWEKPMLKEIEKAILKSDLGINPNNDGTIIRLLVPELTEETRKNLVKNVKKVGEQAKVAIRSIRKDANDKVKNFKKEGTITEDEMKKGEDDIQKVTDKFVKEIDTI 174          
BLAST of CF832535 vs. ExPASy Swiss-Prot
Match: RRF_CLOPE (Ribosome-recycling factor OS=Clostridium perfringens GN=frr PE=3 SV=2)

HSP 1 Score: 148.673 bits (374), Expect = 2.509e-35
Identity = 71/143 (49.65%), Postives = 103/143 (72.03%), Query Frame = -2
Query:  222 SNPAMLDKIEVEYYGSPVSLKSIAQINTPDSSSLLIQPYDKSSLKSIEKAIVSSDLGMTPNNDGEVIRLTLPQLTSERRKELSKVVAKQAEEGKVAVRNIRRDALKAYEKLQKEKKLSEDNVKDLSSDLQKLTDEYMKKIDSI 650
            +NP MLD+I+VEYYGS   L  +A +++P+   L+I P++K  LK IEKAI+ SDLG+ PNNDG +IRL +P+LT E RK L K V K  E+ KVA+R+IR+DA    +  +KE  ++ED +K    D+QK+TD+++K+ID+I
Sbjct:   32 ANPTMLDRIQVEYYGSMCPLNQVANVSSPEPRLLVITPWEKPMLKEIEKAILKSDLGINPNNDGTIIRLLVPELTEETRKNLVKNVKKVGEQAKVAIRSIRKDANDKVKNFKKEGTITEDEMKKGEDDIQKVTDKFVKEIDTI 174          
The following BLAST results are available for this feature:
BLAST of CF832535 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
RRF_CLOBA8.623e-3652.45Ribosome-recycling factor OS=Clostridium botulinum... [more]
RRF_STRSV1.126e-3550.35Ribosome-recycling factor OS=Streptococcus sanguin... [more]
RRF_LACJO1.126e-3547.26Ribosome-recycling factor OS=Lactobacillus johnson... [more]
RRF_EXIS21.126e-3549.65Ribosome-recycling factor OS=Exiguobacterium sibir... [more]
RRF_LACGA1.471e-3547.26Ribosome-recycling factor OS=Lactobacillus gasseri... [more]
RRF_LACCB1.471e-3549.65Ribosome-recycling factor OS=Lactobacillus casei (... [more]
RRF_LACC31.471e-3549.65Ribosome-recycling factor OS=Lactobacillus casei (... [more]
RRF_CLOBB1.471e-3552.45Ribosome-recycling factor OS=Clostridium botulinum... [more]
RRF_CLOPS2.509e-3549.65Ribosome-recycling factor OS=Clostridium perfringe... [more]
RRF_CLOPE2.509e-3549.65Ribosome-recycling factor OS=Clostridium perfringe... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS02_02A06_f Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa02A06, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF832535 ID=CF832535; Name=CF832535; organism=Citrus sinensis; type=EST; length=651bp
TTTTTTTTTTTTTTTTTACAAAGAGTCTAGAATAACTAATTTCCCCCTTT
CATTTCATAACAAAATATCCACCCGTGTCTCCCATTATTATCAAAAAATG
CCATTCTATGGGCAAACCAAAAAATAAAGAGTGCATTCTGAGATTCACTA
GAGAGCTCACCCTTACATTTGTGTGTAAACAATCATTAAACCTTCAACAA
CTCCTTCTCTTTCTGTTTGTAGATGCTATCAATCTTCTTCATATACTCAT
CTGTTAGTTTCTGTAAATCACTTGACAAGTCCTTCACATTGTCTTCAGAG
AGCTTCTTCTCCTTCTGAAGTTTTTCATAAGCTTTTAACGCATCTCTTCT
TATATTCCTAACTGCCACCTTCCCTTCTTCAGCCTGTTTGGCCACAACCT
TTGACAGTTCCTTCCTCCTTTCGGATGTCAATTGAGGAAGGGTTAACCGT
ATGACTTCACCATCATTGTTTGGGGTCATGCCAAGATCAGAGCTCACTAT
GGCCTTCTCTATAGACTTTAAGCTGGATTTGTCATATGGTTGAATCAATA
GAGAACTTGAATCAGGGGTATTGATTTGAGCTATGCTCTTCAAACTTACT
GGGCTTCCATAGTATTCCACCTCAATCTTATCTAGCATAGCTGGATTCGA
T
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