CF832535

Overview
NameCF832535
Unique NameCF832535
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length651
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CF832535 vs. ExPASy Swiss-Prot
Match: RRF_CLOP1 (Ribosome-recycling factor OS=Clostridium perfringens (strain ATCC 13124 / NCTC 8237 / Type A) GN=frr PE=3 SV=1)

HSP 1 Score: 148.673 bits (374), Expect = 2.509e-35
Identity = 71/143 (49.65%), Postives = 103/143 (72.03%), Query Frame = -2
Query:  222 SNPAMLDKIEVEYYGSPVSLKSIAQINTPDSSSLLIQPYDKSSLKSIEKAIVSSDLGMTPNNDGEVIRLTLPQLTSERRKELSKVVAKQAEEGKVAVRNIRRDALKAYEKLQKEKKLSEDNVKDLSSDLQKLTDEYMKKIDSI 650
            +NP MLD+I+VEYYGS   L  +A +++P+   L+I P++K  LK IEKAI+ SDLG+ PNNDG +IRL +P+LT E RK L K V K  E+ KVA+R+IR+DA    +  +KE  ++ED +K    D+QK+TD+++K+ID+I
Sbjct:   32 ANPTMLDRIQVEYYGSMCPLNQVANVSSPEPRLLVITPWEKPMLKEIEKAILKSDLGINPNNDGTIIRLLVPELTEETRKNLVKNVKKVGEQAKVAIRSIRKDANDKVKNFKKEGTITEDEMKKGEDDIQKVTDKFVKEIDTI 174          
BLAST of CF832535 vs. ExPASy Swiss-Prot
Match: RRF_SYNAS (Ribosome-recycling factor OS=Syntrophus aciditrophicus (strain SB) GN=frr PE=3 SV=1)

HSP 1 Score: 148.288 bits (373), Expect = 3.277e-35
Identity = 70/143 (48.95%), Postives = 101/143 (70.63%), Query Frame = -2
Query:  213 AMLDKIEVEYYGSPVSLKSIAQINTPDSSSLLIQPYDKSSLKSIEKAIVSSDLGMTPNNDGEVIRLTLPQLTSERRKELSKVVAKQAEEGKVAVRNIRRDALKAYEKLQKEKKLSEDNVKDLSSDLQKLTDEYMKKIDSIYKQ 641
            ++LD I+ +YYG+P  L  +A ++ P+S  +LI P+D S+L +IEKAI  SDLG+ PNNDG+VIR+++P LT  RRKEL KVV K AEE KV +RN RRDA + ++ ++K   +SED + D    +QK+TD Y++K + I  Q
Sbjct:   35 SLLDGIKADYYGTPTPLNQVATLSVPESRQILISPWDTSALNAIEKAIQKSDLGLVPNNDGKVIRISIPPLTEARRKELVKVVRKMAEECKVKLRNARRDANEEFKSMKKNNDISEDQMHDYQDQVQKMTDSYIEKSEDILVQ 177          
BLAST of CF832535 vs. ExPASy Swiss-Prot
Match: RRF_MOOTA (Ribosome-recycling factor OS=Moorella thermoacetica (strain ATCC 39073) GN=frr PE=3 SV=1)

HSP 1 Score: 148.288 bits (373), Expect = 3.277e-35
Identity = 72/143 (50.35%), Postives = 103/143 (72.03%), Query Frame = -2
Query:  222 SNPAMLDKIEVEYYGSPVSLKSIAQINTPDSSSLLIQPYDKSSLKSIEKAIVSSDLGMTPNNDGEVIRLTLPQLTSERRKELSKVVAKQAEEGKVAVRNIRRDALKAYEKLQKEKKLSEDNVKDLSSDLQKLTDEYMKKIDSI 650
            +NPA+L+K+ V YYG+P  +  +A +++P++  L+IQP+D++ L  IEKAI+ SDLG+TP++DG VIR+T+PQLT ERR EL KV  K+AEE +V VRN RR+A    +  +K K+ SED VK     +QK TD Y++ ID I
Sbjct:   32 ANPALLEKVLVSYYGTPTPVNQLATVSSPEARLLVIQPWDRNILPEIEKAILKSDLGLTPSSDGTVIRITIPQLTEERRSELVKVARKKAEEFRVMVRNARREANDKLKASEKNKEASEDEVKRAQEKVQKTTDNYIQIIDKI 174          
BLAST of CF832535 vs. ExPASy Swiss-Prot
Match: RRF_THET8 (Ribosome-recycling factor OS=Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) GN=frr PE=1 SV=1)

HSP 1 Score: 147.902 bits (372), Expect = 4.280e-35
Identity = 70/143 (48.95%), Postives = 104/143 (72.73%), Query Frame = -2
Query:  222 SNPAMLDKIEVEYYGSPVSLKSIAQINTPDSSSLLIQPYDKSSLKSIEKAIVSSDLGMTPNNDGEVIRLTLPQLTSERRKELSKVVAKQAEEGKVAVRNIRRDALKAYEKLQKEKKLSEDNVKDLSSDLQKLTDEYMKKIDSI 650
            +NPA+L  ++VEYYG+ V L  IA +  PD  +L++Q +D+++LK+IEKAI  SDLG+ P+N G+ + + +P LT ERRK+L + V + AEEG+VA+RNIRR+AL   +KL KE  LSED  K   +++QK+TDE++ K D +
Sbjct:   33 ANPALLLHLKVEYYGAHVPLNQIATVTAPDPRTLVVQSWDQNALKAIEKAIRDSDLGLNPSNKGDALYINIPPLTEERRKDLVRAVRQYAEEGRVAIRNIRREALDKLKKLAKELHLSEDETKRAEAEIQKITDEFIAKADQL 175          
BLAST of CF832535 vs. ExPASy Swiss-Prot
Match: RRF_THET2 (Ribosome-recycling factor OS=Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039) GN=frr PE=3 SV=1)

HSP 1 Score: 147.902 bits (372), Expect = 4.280e-35
Identity = 70/143 (48.95%), Postives = 104/143 (72.73%), Query Frame = -2
Query:  222 SNPAMLDKIEVEYYGSPVSLKSIAQINTPDSSSLLIQPYDKSSLKSIEKAIVSSDLGMTPNNDGEVIRLTLPQLTSERRKELSKVVAKQAEEGKVAVRNIRRDALKAYEKLQKEKKLSEDNVKDLSSDLQKLTDEYMKKIDSI 650
            +NPA+L  ++VEYYG+ V L  IA +  PD  +L++Q +D+++LK+IEKAI  SDLG+ P+N G+ + + +P LT ERRK+L + V + AEEG+VA+RNIRR+AL   +KL KE  LSED  K   +++QK+TDE++ K D +
Sbjct:   33 ANPALLLHLKVEYYGAHVPLNQIATVTAPDPRTLVVQSWDQNALKAIEKAIRDSDLGLNPSNKGDALYINIPPLTEERRKDLVRAVRQYAEEGRVAIRNIRREALDKLKKLAKELHLSEDETKRAEAEIQKITDEFIAKADQL 175          
BLAST of CF832535 vs. ExPASy Swiss-Prot
Match: RRF_DESDG (Ribosome-recycling factor OS=Desulfovibrio desulfuricans (strain G20) GN=frr PE=3 SV=1)

HSP 1 Score: 147.902 bits (372), Expect = 4.280e-35
Identity = 66/143 (46.15%), Postives = 106/143 (74.13%), Query Frame = -2
Query:  222 SNPAMLDKIEVEYYGSPVSLKSIAQINTPDSSSLLIQPYDKSSLKSIEKAIVSSDLGMTPNNDGEVIRLTLPQLTSERRKELSKVVAKQAEEGKVAVRNIRRDALKAYEKLQKEKKLSEDNVKDLSSDLQKLTDEYMKKIDSI 650
            ++ +++D I+V+YYG+P  +  +A +  PDS ++ IQP+D+ +   +EKAI+ SDLG+TP NDG++IR+++P LT ERRKEL KV  K  E+ KVAVRNIRRDA +  +K++K+K ++ED++K    ++QKLTD ++ K + +
Sbjct:   31 ASTSLVDSIKVDYYGTPTPINQLASVAVPDSRTITIQPWDRGAFALVEKAIMQSDLGLTPINDGKIIRISMPPLTEERRKELVKVAKKYTEDAKVAVRNIRRDANEQIKKMEKDKAITEDDMKRGQDEVQKLTDAFVAKSEKV 173          
BLAST of CF832535 vs. ExPASy Swiss-Prot
Match: RRF_SHEAM (Ribosome-recycling factor OS=Shewanella amazonensis (strain ATCC BAA-1098 / SB2B) GN=frr PE=3 SV=1)

HSP 1 Score: 147.132 bits (370), Expect = 7.300e-35
Identity = 66/143 (46.15%), Postives = 108/143 (75.52%), Query Frame = -2
Query:  222 SNPAMLDKIEVEYYGSPVSLKSIAQINTPDSSSLLIQPYDKSSLKSIEKAIVSSDLGMTPNNDGEVIRLTLPQLTSERRKELSKVVAKQAEEGKVAVRNIRRDALKAYEKLQKEKKLSEDNVKDLSSDLQKLTDEYMKKIDSI 650
            ++P++LD I+V YYGS   LK +  +   DS +L +  +D+S+++++EKAI+SSDLG+ P + G  IR+ LP LT ERR++L KVV  +AE+G+VA+RN+RRDA  ++++L+KEK+ +ED+V+    ++QK+TD ++KKID +
Sbjct:   32 AHPSLLDTIQVNYYGSMTPLKQVGNVTIEDSRTLAVNVFDRSAIQAVEKAIMSSDLGLNPMSAGATIRIPLPPLTEERRRDLVKVVRAEAEQGRVAIRNVRRDANSSFKQLEKEKECTEDDVRRSEEEVQKITDLHIKKIDEL 174          
BLAST of CF832535 vs. ExPASy Swiss-Prot
Match: RRF_FUSNN (Ribosome-recycling factor OS=Fusobacterium nucleatum subsp. nucleatum GN=frr PE=3 SV=1)

HSP 1 Score: 147.132 bits (370), Expect = 7.300e-35
Identity = 71/145 (48.97%), Postives = 108/145 (74.48%), Query Frame = -2
Query:  222 SNPAMLDKIEVEYYGSPVSLKSIAQINTPDSSSLLIQPYDKSSLKSIEKAIVSSDLGMTPNNDGEVIRLTLPQLTSERRKELSKVVAKQAEEGKVAVRNIRRDALKAYEKLQKEKK--LSEDNVKDLSSDLQKLTDEYMKKIDSI 650
            +N +MLD I+VE YGS V L  I  ++ P++  L+I P+DK+ +  IEKAI+++++GMTPNNDG VIRL LP+LT++RRKE  K+   +AE GK+A+RNIR+D     +KL+K+K+  +SED +K   +++Q LTD+Y+K+ID +
Sbjct:   35 ANVSMLDGIKVENYGSEVPLNQIGTVSAPEARLLVIDPWDKTLISKIEKAILAANIGMTPNNDGRVIRLVLPELTADRRKEYVKLAKNEAENGKIAIRNIRKDINNHLKKLEKDKENPISEDELKKEETNVQTLTDKYVKEIDDL 179          
BLAST of CF832535 vs. ExPASy Swiss-Prot
Match: RRF_LEUCK (Ribosome-recycling factor OS=Leuconostoc citreum (strain KM20) GN=frr PE=3 SV=1)

HSP 1 Score: 146.747 bits (369), Expect = 9.534e-35
Identity = 70/143 (48.95%), Postives = 107/143 (74.83%), Query Frame = -2
Query:  222 SNPAMLDKIEVEYYGSPVSLKSIAQINTPDSSSLLIQPYDKSSLKSIEKAIVSSDLGMTPNNDGEVIRLTLPQLTSERRKELSKVVAKQAEEGKVAVRNIRRDALKAYEKLQKEKKLSEDNVKDLSSDLQKLTDEYMKKIDSI 650
            +NP +LD+IEVEYYG+   L  +A I+ P++  LLI P+DK++L+ I +AI  SDLG+ P++DG ++RL +PQ+T E RK+L+K V  +AE+ KV+VRN+RRDA+   + ++KEK++ ED+V+   +D+QKLTD  +K ID I
Sbjct:   31 ANPHILDRIEVEYYGAMTPLNQVASISVPEARVLLITPFDKTALEEIIRAINMSDLGLNPSSDGNIVRLMIPQMTEEGRKDLAKQVKAEAEKAKVSVRNVRRDAM---DSVKKEKEMPEDDVRKAENDIQKLTDNNIKAIDDI 170          
BLAST of CF832535 vs. ExPASy Swiss-Prot
Match: RRF_SYNFM (Ribosome-recycling factor OS=Syntrophobacter fumaroxidans (strain DSM 10017 / MPOB) GN=frr PE=3 SV=1)

HSP 1 Score: 146.362 bits (368), Expect = 1.245e-34
Identity = 68/141 (48.23%), Postives = 104/141 (73.76%), Query Frame = -2
Query:  219 AMLDKIEVEYYGSPVSLKSIAQINTPDSSSLLIQPYDKSSLKSIEKAIVSSDLGMTPNNDGEVIRLTLPQLTSERRKELSKVVAKQAEEGKVAVRNIRRDALKAYEKLQKEKKLSEDNVKDLSSDLQKLTDEYMKKIDSIY 641
            +++D I  EYYG+P +L  +A I  P+  +++IQP+D S +  IEK+I+ S+LG+TP +DG+VIR+ +P LT++RR+EL KVV K +EE KVAVRNIRRD  +  + L+KEK++SED       + Q++TD+++KKID +Y
Sbjct:   35 SLVDGIRAEYYGTPTALNQLATITIPEPRTIMIQPWDTSVIGEIEKSILKSELGLTPMSDGKVIRINIPVLTADRRRELVKVVKKMSEESKVAVRNIRRDVNEMIKDLKKEKEISEDEQFKAQEETQRITDDFIKKIDVVY 175          
The following BLAST results are available for this feature:
BLAST of CF832535 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
RRF_CLOP12.509e-3549.65Ribosome-recycling factor OS=Clostridium perfringe... [more]
RRF_SYNAS3.277e-3548.95Ribosome-recycling factor OS=Syntrophus aciditroph... [more]
RRF_MOOTA3.277e-3550.35Ribosome-recycling factor OS=Moorella thermoacetic... [more]
RRF_THET84.280e-3548.95Ribosome-recycling factor OS=Thermus thermophilus ... [more]
RRF_THET24.280e-3548.95Ribosome-recycling factor OS=Thermus thermophilus ... [more]
RRF_DESDG4.280e-3546.15Ribosome-recycling factor OS=Desulfovibrio desulfu... [more]
RRF_SHEAM7.300e-3546.15Ribosome-recycling factor OS=Shewanella amazonensi... [more]
RRF_FUSNN7.300e-3548.97Ribosome-recycling factor OS=Fusobacterium nucleat... [more]
RRF_LEUCK9.534e-3548.95Ribosome-recycling factor OS=Leuconostoc citreum (... [more]
RRF_SYNFM1.245e-3448.23Ribosome-recycling factor OS=Syntrophobacter fumar... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS02_02A06_f Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa02A06, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF832535 ID=CF832535; Name=CF832535; organism=Citrus sinensis; type=EST; length=651bp
TTTTTTTTTTTTTTTTTACAAAGAGTCTAGAATAACTAATTTCCCCCTTT
CATTTCATAACAAAATATCCACCCGTGTCTCCCATTATTATCAAAAAATG
CCATTCTATGGGCAAACCAAAAAATAAAGAGTGCATTCTGAGATTCACTA
GAGAGCTCACCCTTACATTTGTGTGTAAACAATCATTAAACCTTCAACAA
CTCCTTCTCTTTCTGTTTGTAGATGCTATCAATCTTCTTCATATACTCAT
CTGTTAGTTTCTGTAAATCACTTGACAAGTCCTTCACATTGTCTTCAGAG
AGCTTCTTCTCCTTCTGAAGTTTTTCATAAGCTTTTAACGCATCTCTTCT
TATATTCCTAACTGCCACCTTCCCTTCTTCAGCCTGTTTGGCCACAACCT
TTGACAGTTCCTTCCTCCTTTCGGATGTCAATTGAGGAAGGGTTAACCGT
ATGACTTCACCATCATTGTTTGGGGTCATGCCAAGATCAGAGCTCACTAT
GGCCTTCTCTATAGACTTTAAGCTGGATTTGTCATATGGTTGAATCAATA
GAGAACTTGAATCAGGGGTATTGATTTGAGCTATGCTCTTCAAACTTACT
GGGCTTCCATAGTATTCCACCTCAATCTTATCTAGCATAGCTGGATTCGA
T
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