CF832535

Overview
NameCF832535
Unique NameCF832535
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length651
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CF832535 vs. ExPASy Swiss-Prot
Match: RRF_GEOKA (Ribosome-recycling factor OS=Geobacillus kaustophilus GN=frr PE=3 SV=1)

HSP 1 Score: 164.081 bits (414), Expect = 5.771e-40
Identity = 75/146 (51.37%), Postives = 114/146 (78.08%), Query Frame = -2
Query:  213 SNPAMLDKIEVEYYGSPVSLKSIAQINTPDSSSLLIQPYDKSSLKSIEKAIVSSDLGMTPNNDGEVIRLTLPQLTSERRKELSKVVAKQAEEGKVAVRNIRRDALKAYEKLQKEKKLSEDNVKDLSSDLQKLTDEYMKKIDSIYKQ 650
            +N  +L+K+ V+YYG P  +  +A I+ P++  L+IQPYDKS++K +EKAI++SDLG+TP+NDG VIRL +P LT ERR+EL+K+V K +E+ KVAVRNIRRDA    +KL+K  +++ED ++  + ++QKLTD+++ KID+I K+
Sbjct:   32 ANAGLLEKVTVDYYGVPTPINQLASISVPEARLLVIQPYDKSAIKEMEKAILASDLGLTPSNDGSVIRLVIPPLTEERRRELAKLVKKYSEDAKVAVRNIRRDANDELKKLEKNGEITEDELRSYTDEVQKLTDDHIAKIDAITKE 177          
BLAST of CF832535 vs. ExPASy Swiss-Prot
Match: RRF_LISIN (Ribosome-recycling factor OS=Listeria innocua GN=frr PE=3 SV=1)

HSP 1 Score: 163.696 bits (413), Expect = 7.537e-40
Identity = 80/145 (55.17%), Postives = 110/145 (75.86%), Query Frame = -2
Query:  216 SNPAMLDKIEVEYYGSPVSLKSIAQINTPDSSSLLIQPYDKSSLKSIEKAIVSSDLGMTPNNDGEVIRLTLPQLTSERRKELSKVVAKQAEEGKVAVRNIRRDALKAYEKLQKEKKLSEDNVKDLSSDLQKLTDEYMKKIDSIYK 650
            +N ++LD++ V+YYG+   +  +A I+ P++  LLI PYDK+ L  IEKAI+ SDLG+TPNNDG V+RL++PQLT ERRKEL K V K+AEE KVAVRNIRR+A +  +KL+K   ++ED+++    D+QKLTDE +K IDSI K
Sbjct:   32 ANASLLDRLTVDYYGAATPVNQMASISVPEARMLLITPYDKTILGEIEKAILKSDLGLTPNNDGSVLRLSIPQLTEERRKELVKEVKKEAEEAKVAVRNIRREANEDLKKLEKNGDITEDDLRSYGEDVQKLTDESIKNIDSITK 176          
BLAST of CF832535 vs. ExPASy Swiss-Prot
Match: RRF_SYNWW (Ribosome-recycling factor OS=Syntrophomonas wolfei subsp. wolfei (strain Goettingen) GN=frr PE=3 SV=1)

HSP 1 Score: 163.31 bits (412), Expect = 9.843e-40
Identity = 81/143 (56.64%), Postives = 109/143 (76.22%), Query Frame = -2
Query:  222 SNPAMLDKIEVEYYGSPVSLKSIAQINTPDSSSLLIQPYDKSSLKSIEKAIVSSDLGMTPNNDGEVIRLTLPQLTSERRKELSKVVAKQAEEGKVAVRNIRRDALKAYEKLQKEKKLSEDNVKDLSSDLQKLTDEYMKKIDSI 650
            +NPAML+KI V+YYG P  +  +A I  P++  L+IQP+DK+ + SIEKAI+ SDLG+ P+NDG VIRL +PQLT ERRKEL KV+ K+AEE +VAVRNIRRD+ +  +  +KEK +SED+ K    D+QK TD ++K+IDSI
Sbjct:   32 ANPAMLEKIMVDYYGQPTPINQLANITVPEARLLVIQPWDKTIIASIEKAIMKSDLGINPSNDGNVIRLVIPQLTEERRKELVKVLRKRAEEARVAVRNIRRDSNELLKSGEKEKLISEDDNKKGMDDIQKETDRHIKEIDSI 174          
BLAST of CF832535 vs. ExPASy Swiss-Prot
Match: RRF_PROM4 (Ribosome-recycling factor OS=Prochlorococcus marinus (strain MIT 9211) GN=frr PE=3 SV=1)

HSP 1 Score: 163.31 bits (412), Expect = 9.843e-40
Identity = 77/141 (54.61%), Postives = 109/141 (77.30%), Query Frame = -2
Query:  228 SNPAMLDKIEVEYYGSPVSLKSIAQINTPDSSSLLIQPYDKSSLKSIEKAIVSSDLGMTPNNDGEVIRLTLPQLTSERRKELSKVVAKQAEEGKVAVRNIRRDALKAYEKLQKEKKLSEDNVKDLSSDLQKLTDEYMKKID 650
            +N ++LD++ VEYYG+   LKS+A I+TPDS ++ IQP+D SSL  IEK+I  SDLG TPNNDG++IR+ +P LT ERRKE  K+ +K AEEGKVA+RNIRRDA++  +K +KE ++SED  +D   ++QKLTD ++ +I+
Sbjct:   29 ANTSLLDRLTVEYYGADTPLKSLATISTPDSQTISIQPFDLSSLVLIEKSIAMSDLGFTPNNDGKIIRINIPPLTEERRKEFCKLASKYAEEGKVALRNIRRDAIERIKKSEKESEISEDQSRDEQDNVQKLTDRFITEIE 169          
BLAST of CF832535 vs. ExPASy Swiss-Prot
Match: RRF_ALKMQ (Ribosome-recycling factor OS=Alkaliphilus metalliredigens (strain QYMF) GN=frr PE=3 SV=1)

HSP 1 Score: 163.31 bits (412), Expect = 9.843e-40
Identity = 74/143 (51.75%), Postives = 112/143 (78.32%), Query Frame = -2
Query:  222 SNPAMLDKIEVEYYGSPVSLKSIAQINTPDSSSLLIQPYDKSSLKSIEKAIVSSDLGMTPNNDGEVIRLTLPQLTSERRKELSKVVAKQAEEGKVAVRNIRRDALKAYEKLQKEKKLSEDNVKDLSSDLQKLTDEYMKKIDSI 650
            +NP+MLD+I V+YYGS   LK +A ++ P+   L+IQPYD S +  IEKA++ SDLG+ P+NDG++IRL +PQLT ERRKEL+KVV + AEEG+VA+RN RRDA +  +K++K+ +L+ED++K    ++QK+TD++ K++D +
Sbjct:   32 ANPSMLDRITVDYYGSITPLKQVASVSAPEPRLLIIQPYDPSVINGIEKALMMSDLGLNPSNDGKIIRLNIPQLTEERRKELTKVVKRVAEEGRVALRNGRRDANEQLKKMEKDSELTEDDLKQAQEEVQKITDKFNKQVDEM 174          
BLAST of CF832535 vs. ExPASy Swiss-Prot
Match: RRF_LACS1 (Ribosome-recycling factor OS=Lactobacillus salivarius subsp. salivarius (strain UCC118) GN=frr PE=3 SV=1)

HSP 1 Score: 162.925 bits (411), Expect = 1.286e-39
Identity = 80/143 (55.94%), Postives = 108/143 (75.52%), Query Frame = -2
Query:  222 SNPAMLDKIEVEYYGSPVSLKSIAQINTPDSSSLLIQPYDKSSLKSIEKAIVSSDLGMTPNNDGEVIRLTLPQLTSERRKELSKVVAKQAEEGKVAVRNIRRDALKAYEKLQKEKKLSEDNVKDLSSDLQKLTDEYMKKIDSI 650
            +N ++L++I VEYYG+P  L  +AQI+ P++  LL+ PYDK+SLK+IE AI++SDLG+ P NDG  IRL +PQLT ERRKEL+K V   +E GKVAVRNIRRD + A +K QK   L+ED+++DL +  QK+TDE +K ID I
Sbjct:   34 ANASLLNRINVEYYGAPTPLNQMAQISVPEARVLLVTPYDKTSLKNIEHAIMASDLGIAPMNDGTAIRLVIPQLTEERRKELAKQVKAVSETGKVAVRNIRRDMMDALKKAQKNGDLTEDDLRDLENQAQKVTDESIKNIDKI 176          
BLAST of CF832535 vs. ExPASy Swiss-Prot
Match: RRF_SYMTH (Ribosome-recycling factor OS=Symbiobacterium thermophilum GN=frr PE=3 SV=1)

HSP 1 Score: 162.54 bits (410), Expect = 1.679e-39
Identity = 76/143 (53.15%), Postives = 112/143 (78.32%), Query Frame = -2
Query:  222 SNPAMLDKIEVEYYGSPVSLKSIAQINTPDSSSLLIQPYDKSSLKSIEKAIVSSDLGMTPNNDGEVIRLTLPQLTSERRKELSKVVAKQAEEGKVAVRNIRRDALKAYEKLQKEKKLSEDNVKDLSSDLQKLTDEYMKKIDSI 650
            + PA+LDK+ VE YGS V + ++A I  PD  +L+I+P+D+S +K+IE+AI +SDLG+ P NDG+VIRL++P +T ERR+EL KVVAK+ EE +VA+RNIRRDA +  +KL+K+K +SED  K    ++QKLTD+Y+K++D +
Sbjct:   32 ATPALLDKVRVEAYGSEVPVNNVATIEVPDPRTLVIKPWDRSLIKAIERAINASDLGLNPTNDGQVIRLSIPPMTEERRRELVKVVAKRTEEQRVAIRNIRRDANEQIKKLEKDKAVSEDESKRAQDEVQKLTDKYIKEVDQL 174          
BLAST of CF832535 vs. ExPASy Swiss-Prot
Match: RRF_GLOVI (Ribosome-recycling factor OS=Gloeobacter violaceus GN=frr PE=3 SV=1)

HSP 1 Score: 162.54 bits (410), Expect = 1.679e-39
Identity = 77/145 (53.10%), Postives = 112/145 (77.24%), Query Frame = -2
Query:  216 SNPAMLDKIEVEYYGSPVSLKSIAQINTPDSSSLLIQPYDKSSLKSIEKAIVSSDLGMTPNNDGEVIRLTLPQLTSERRKELSKVVAKQAEEGKVAVRNIRRDALKAYEKLQKEKKLSEDNVKDLSSDLQKLTDEYMKKIDSIYK 650
            ++ ++LD+I VEYYG P  LK++A I TPD+S++LIQPYD SSL+ IEK I+ SDLG+ P+NDG+ IRL +P LT+ERRK+L KV+   AEEG+VAVRNIRR A+    K +K+ K+SED  + L  ++QKLTD+ +++I+ +++
Sbjct:   29 ASTSLLDRINVEYYGQPTPLKTLATITTPDASTVLIQPYDPSSLRLIEKTILESDLGLPPSNDGKTIRLNIPPLTAERRKDLVKVLRNLAEEGRVAVRNIRRHAIDEVRKEEKDAKVSEDEARRLQDEVQKLTDKSIQQIEKLFE 173          
BLAST of CF832535 vs. ExPASy Swiss-Prot
Match: RRF_PELTS (Ribosome-recycling factor OS=Pelotomaculum thermopropionicum (strain DSM 13744 / JCM 10971 / SI) GN=frr PE=3 SV=1)

HSP 1 Score: 162.155 bits (409), Expect = 2.193e-39
Identity = 76/143 (53.15%), Postives = 108/143 (75.52%), Query Frame = -2
Query:  222 SNPAMLDKIEVEYYGSPVSLKSIAQINTPDSSSLLIQPYDKSSLKSIEKAIVSSDLGMTPNNDGEVIRLTLPQLTSERRKELSKVVAKQAEEGKVAVRNIRRDALKAYEKLQKEKKLSEDNVKDLSSDLQKLTDEYMKKIDSI 650
            + PA+LDK+ V YYG+P  +  +A I+ P++  L++QP+DK+ L  IE+AI+ SDLG+TP +DG VIRL +PQLT ERR EL KV+ K+AEEG+VA+RNIRRD  +  +  QK+ K+SED +K    ++QKLTD Y+K+IDS+
Sbjct:   32 ATPALLDKVMVNYYGTPTPVNQLANISVPEARLLVVQPWDKTVLPEIERAILKSDLGITPASDGNVIRLAIPQLTQERRAELMKVIKKKAEEGRVAIRNIRRDTNEQLKSQQKDGKMSEDELKRSQDEVQKLTDRYIKEIDSL 174          
BLAST of CF832535 vs. ExPASy Swiss-Prot
Match: RRF_ENTFA (Ribosome-recycling factor OS=Enterococcus faecalis GN=frr PE=3 SV=1)

HSP 1 Score: 162.155 bits (409), Expect = 2.193e-39
Identity = 76/143 (53.15%), Postives = 107/143 (74.83%), Query Frame = -2
Query:  222 SNPAMLDKIEVEYYGSPVSLKSIAQINTPDSSSLLIQPYDKSSLKSIEKAIVSSDLGMTPNNDGEVIRLTLPQLTSERRKELSKVVAKQAEEGKVAVRNIRRDALKAYEKLQKEKKLSEDNVKDLSSDLQKLTDEYMKKIDSI 650
            +N ++LD+I+VEYYG+P  +  +A IN P++  L+I P+DK+S+  IEKAI  SD+G++P NDG VIRL +PQLT ERRKEL+K V K+AE  KVAVRN+RRDA+   +K QK   ++ED ++    D+QKLTD+ +K ID+I
Sbjct:   32 ANASLLDRIQVEYYGAPTPVNQLASINIPEARVLMITPFDKNSIADIEKAIQMSDIGISPTNDGNVIRLVIPQLTEERRKELAKDVKKEAENSKVAVRNVRRDAMDELKKAQKNGDITEDELRSFEKDVQKLTDDSIKNIDAI 174          
The following BLAST results are available for this feature:
BLAST of CF832535 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
RRF_GEOKA5.771e-4051.37Ribosome-recycling factor OS=Geobacillus kaustophi... [more]
RRF_LISIN7.537e-4055.17Ribosome-recycling factor OS=Listeria innocua GN=f... [more]
RRF_SYNWW9.843e-4056.64Ribosome-recycling factor OS=Syntrophomonas wolfei... [more]
RRF_PROM49.843e-4054.61Ribosome-recycling factor OS=Prochlorococcus marin... [more]
RRF_ALKMQ9.843e-4051.75Ribosome-recycling factor OS=Alkaliphilus metallir... [more]
RRF_LACS11.286e-3955.94Ribosome-recycling factor OS=Lactobacillus salivar... [more]
RRF_SYMTH1.679e-3953.15Ribosome-recycling factor OS=Symbiobacterium therm... [more]
RRF_GLOVI1.679e-3953.10Ribosome-recycling factor OS=Gloeobacter violaceus... [more]
RRF_PELTS2.193e-3953.15Ribosome-recycling factor OS=Pelotomaculum thermop... [more]
RRF_ENTFA2.193e-3953.15Ribosome-recycling factor OS=Enterococcus faecalis... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS02_02A06_f Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa02A06, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF832535 ID=CF832535; Name=CF832535; organism=Citrus sinensis; type=EST; length=651bp
TTTTTTTTTTTTTTTTTACAAAGAGTCTAGAATAACTAATTTCCCCCTTT
CATTTCATAACAAAATATCCACCCGTGTCTCCCATTATTATCAAAAAATG
CCATTCTATGGGCAAACCAAAAAATAAAGAGTGCATTCTGAGATTCACTA
GAGAGCTCACCCTTACATTTGTGTGTAAACAATCATTAAACCTTCAACAA
CTCCTTCTCTTTCTGTTTGTAGATGCTATCAATCTTCTTCATATACTCAT
CTGTTAGTTTCTGTAAATCACTTGACAAGTCCTTCACATTGTCTTCAGAG
AGCTTCTTCTCCTTCTGAAGTTTTTCATAAGCTTTTAACGCATCTCTTCT
TATATTCCTAACTGCCACCTTCCCTTCTTCAGCCTGTTTGGCCACAACCT
TTGACAGTTCCTTCCTCCTTTCGGATGTCAATTGAGGAAGGGTTAACCGT
ATGACTTCACCATCATTGTTTGGGGTCATGCCAAGATCAGAGCTCACTAT
GGCCTTCTCTATAGACTTTAAGCTGGATTTGTCATATGGTTGAATCAATA
GAGAACTTGAATCAGGGGTATTGATTTGAGCTATGCTCTTCAAACTTACT
GGGCTTCCATAGTATTCCACCTCAATCTTATCTAGCATAGCTGGATTCGA
T
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