CF832573

Overview
NameCF832573
Unique NameCF832573
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length788
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CF832573 vs. ExPASy Swiss-Prot
Match: METE_ECODH (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Escherichia coli (strain K12 / DH10B) GN=metE PE=3 SV=1)

HSP 1 Score: 210.69 bits (535), Expect = 7.504e-54
Identity = 112/191 (58.64%), Postives = 129/191 (67.54%), Query Frame = -2
Query:  215 SFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAKLLR 787
            SF R D  R     QIALA++DEV DLE AGI +IQIDE ALREGLPLR+S+   YL W V +FRI     +D TQIHTHMCY  FNDI+ SI  +DADVITIE SRSD +LL  F E   Y   IGPGVYDIHSP +PS E I   + K    +    LWVNPDCGLKTR + E + AL+NMV AA+ LR
Sbjct:  562 SFPREDVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEAFRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEE-FDYPNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAALANMVQAAQNLR 751          
BLAST of CF832573 vs. ExPASy Swiss-Prot
Match: METE_ECO8A (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Escherichia coli O8 (strain IAI1) GN=metE PE=3 SV=1)

HSP 1 Score: 210.69 bits (535), Expect = 7.504e-54
Identity = 112/191 (58.64%), Postives = 129/191 (67.54%), Query Frame = -2
Query:  215 SFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAKLLR 787
            SF R D  R     QIALA++DEV DLE AGI +IQIDE ALREGLPLR+S+   YL W V +FRI     +D TQIHTHMCY  FNDI+ SI  +DADVITIE SRSD +LL  F E   Y   IGPGVYDIHSP +PS E I   + K    +    LWVNPDCGLKTR + E + AL+NMV AA+ LR
Sbjct:  562 SFPREDVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEAFRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEE-FDYPNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAALANMVQAAQNLR 751          
BLAST of CF832573 vs. ExPASy Swiss-Prot
Match: METE_ECO7I (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Escherichia coli O7:K1 (strain IAI39 / ExPEC) GN=metE PE=3 SV=1)

HSP 1 Score: 210.69 bits (535), Expect = 7.504e-54
Identity = 112/191 (58.64%), Postives = 129/191 (67.54%), Query Frame = -2
Query:  215 SFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAKLLR 787
            SF R D  R     QIALA++DEV DLE AGI +IQIDE ALREGLPLR+S+   YL W V +FRI     +D TQIHTHMCY  FNDI+ SI  +DADVITIE SRSD +LL  F E   Y   IGPGVYDIHSP +PS E I   + K    +    LWVNPDCGLKTR + E + AL+NMV AA+ LR
Sbjct:  562 SFPREDVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEAFRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEE-FDYPNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAALANMVQAAQNLR 751          
BLAST of CF832573 vs. ExPASy Swiss-Prot
Match: METE_ECO5E (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Escherichia coli O157:H7 (strain EC4115 / EHEC) GN=metE PE=3 SV=1)

HSP 1 Score: 210.69 bits (535), Expect = 7.504e-54
Identity = 112/191 (58.64%), Postives = 129/191 (67.54%), Query Frame = -2
Query:  215 SFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAKLLR 787
            SF R D  R     QIALA++DEV DLE AGI +IQIDE ALREGLPLR+S+   YL W V +FRI     +D TQIHTHMCY  FNDI+ SI  +DADVITIE SRSD +LL  F E   Y   IGPGVYDIHSP +PS E I   + K    +    LWVNPDCGLKTR + E + AL+NMV AA+ LR
Sbjct:  562 SFPREDVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEAFRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEE-FDYPNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAALANMVQAAQNLR 751          
BLAST of CF832573 vs. ExPASy Swiss-Prot
Match: METE_ECO57 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Escherichia coli O157:H7 GN=metE PE=3 SV=3)

HSP 1 Score: 210.69 bits (535), Expect = 7.504e-54
Identity = 112/191 (58.64%), Postives = 129/191 (67.54%), Query Frame = -2
Query:  215 SFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAKLLR 787
            SF R D  R     QIALA++DEV DLE AGI +IQIDE ALREGLPLR+S+   YL W V +FRI     +D TQIHTHMCY  FNDI+ SI  +DADVITIE SRSD +LL  F E   Y   IGPGVYDIHSP +PS E I   + K    +    LWVNPDCGLKTR + E + AL+NMV AA+ LR
Sbjct:  562 SFPREDVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEAFRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEE-FDYPNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAALANMVQAAQNLR 751          
BLAST of CF832573 vs. ExPASy Swiss-Prot
Match: METE_ECO45 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Escherichia coli O45:K1 (strain S88 / ExPEC) GN=metE PE=3 SV=1)

HSP 1 Score: 210.69 bits (535), Expect = 7.504e-54
Identity = 112/191 (58.64%), Postives = 129/191 (67.54%), Query Frame = -2
Query:  215 SFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAKLLR 787
            SF R D  R     QIALA++DEV DLE AGI +IQIDE ALREGLPLR+S+   YL W V +FRI     +D TQIHTHMCY  FNDI+ SI  +DADVITIE SRSD +LL  F E   Y   IGPGVYDIHSP +PS E I   + K    +    LWVNPDCGLKTR + E + AL+NMV AA+ LR
Sbjct:  562 SFPREDVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEAFRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEE-FDYPNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAALANMVQAAQNLR 751          
BLAST of CF832573 vs. ExPASy Swiss-Prot
Match: METE_ECO24 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Escherichia coli O139:H28 (strain E24377A / ETEC) GN=metE PE=3 SV=1)

HSP 1 Score: 210.69 bits (535), Expect = 7.504e-54
Identity = 112/191 (58.64%), Postives = 129/191 (67.54%), Query Frame = -2
Query:  215 SFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAKLLR 787
            SF R D  R     QIALA++DEV DLE AGI +IQIDE ALREGLPLR+S+   YL W V +FRI     +D TQIHTHMCY  FNDI+ SI  +DADVITIE SRSD +LL  F E   Y   IGPGVYDIHSP +PS E I   + K    +    LWVNPDCGLKTR + E + AL+NMV AA+ LR
Sbjct:  562 SFPREDVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEAFRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEE-FDYPNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAALANMVQAAQNLR 751          
BLAST of CF832573 vs. ExPASy Swiss-Prot
Match: METE_STRCO (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Streptomyces coelicolor GN=metE PE=3 SV=1)

HSP 1 Score: 210.305 bits (534), Expect = 9.800e-54
Identity = 106/199 (53.27%), Postives = 139/199 (69.85%), Query Frame = -2
Query:  197 SFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYG--AGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAKLLRTQLASA 787
            SFVR+DQP  +T  Q+ALA++DEV DLE AG +VIQ+DE ALRE LPLR ++H  YL WA  +FR+T  GV+  TQIHTHMCY+ F DI+ +I D+DADVI++E +RS    + V  E   +G     GPGVYDIHSPR+PS EE A  +   L  +    LWVNPDCGLKTR + E + +L N+VA A+ LR +L+++
Sbjct:  577 SFVRDDQPLGDTARQVALALRDEVNDLEAAGTSVIQVDEPALRETLPLRAADHTAYLAWATEAFRLTTSGVRPDTQIHTHMCYAEFGDIVQAIDDLDADVISLEAARSH---MQVAHELATHGYPREAGPGVYDIHSPRVPSAEEAAALLRTGLKAIPAERLWVNPDCGLKTRGWPETRASLENLVATARTLRGELSAS 772          
BLAST of CF832573 vs. ExPASy Swiss-Prot
Match: METE_SALAR (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Salmonella arizonae (strain ATCC BAA-731 / CDC346-86 / RSK2980) GN=metE PE=3 SV=1)

HSP 1 Score: 210.305 bits (534), Expect = 9.800e-54
Identity = 112/191 (58.64%), Postives = 128/191 (67.02%), Query Frame = -2
Query:  215 SFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAKLLR 787
            SF R D  R     QIALA++DEV DLE AGI +IQIDE ALREGLPLR+S+   YL W V +FRI     +D TQIHTHMCY  FNDI+ SI  +DADVITIE SRSD +LL  F E   Y   IGPGVYDIHSP +PS E I   + K    +    LWVNPDCGLKTR + E + AL+NMV AA  LR
Sbjct:  562 SFPREDVTRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLAWGVEAFRINAAAAKDETQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESF-EAFDYPNEIGPGVYDIHSPNVPSVEWIEALLKKAAQRIPAQRLWVNPDCGLKTRGWPETRAALANMVKAAHNLR 751          
BLAST of CF832573 vs. ExPASy Swiss-Prot
Match: METE_RHOSR (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Rhodococcus sp. (strain RHA1) GN=metE PE=3 SV=1)

HSP 1 Score: 210.305 bits (534), Expect = 9.800e-54
Identity = 103/194 (53.09%), Postives = 138/194 (71.13%), Query Frame = -2
Query:  206 SFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAKLLRTQL 787
            SFVR+DQP  ++  Q+ALAI+DE  DL+ AGI ++Q+DE ALRE LPLR ++   YLDW+V +FR+   GV D+TQIHTH+CYS F ++I +I  +DADV +IE +RS  ++L      V +  G+GPGVYDIHSPR+P  EEIA  + + L  +    LWVNPDCGLKTR   EV+ +L N+V AAKL+R +L
Sbjct:  561 SFVRDDQPLADSANQVALAIRDETVDLQGAGIRIVQVDEPALRELLPLRAADQPGYLDWSVGAFRLATSGVSDSTQIHTHLCYSEFGEVIEAIARLDADVTSIEAARSHMEVLDDL-SAVGFDLGVGPGVYDIHSPRVPGVEEIAASLREALKAVPVERLWVNPDCGLKTRGPAEVEASLRNLVDAAKLVRAEL 753          
The following BLAST results are available for this feature:
BLAST of CF832573 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 283
Match NameE-valueIdentityDescription
METE_ECODH7.504e-5458.645-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_ECO8A7.504e-5458.645-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_ECO7I7.504e-5458.645-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_ECO5E7.504e-5458.645-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_ECO577.504e-5458.645-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_ECO457.504e-5458.645-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_ECO247.504e-5458.645-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_STRCO9.800e-5453.275-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_SALAR9.800e-5458.645-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_RHOSR9.800e-5453.095-methyltetrahydropteroyltriglutamate--homocystein... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS02_02B06_f Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa02B06, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF832573 ID=CF832573; Name=CF832573; organism=Citrus sinensis; type=EST; length=788bp
TTTTTTTTTTTTTTTAGAAAATAGAAGCCAATTGCTCAATAGTGCATAAT
ATGCATACGGAAAATTTTCATCACATCACGACCCACAGTTATTCGAATAG
GTAATAAGACAGGAATTTTCTTTCTTTGTTTAAAGTGAAAAAAAAAAAAA
TCAATGGCTCAGGAAGTATCCCTCCAATCTCCAGAACCACTCACTTGGCA
CTGGCAAGCTGGGTGCGGAGTAGCTTGGCAGCAGCGACCATGTTTGAGAG
GGCTGGCTTCACCTCAGTGTACTTGCGAGTCTTAAGACCACAGTCAGGGT
TAACCCACAAAATGTTGGTTTCTAGCACAGCAAGCATCTTGTTGATTCTG
TCAGCAATTTCTTCAGTTGATGGTATTCTTGGAGAGTGGATGTCATACAC
ACCAGGGCCAATTCCAGCACCATACTTGACTCCCTCACGGAAGACAGACA
GGAGCTTCTCATCAGAACGAGAGTTCTCAATGGTGATCACATCAGCATCC
ATGTCGATAATTGAGTGGATAATGTCGTTGAAGTTGGAGTAGCACATGTG
GGTGTGGATCTGGGTAGTGTCCTGGACGCCACAGTTGGTGATTCTGAAGG
AGTGGACTGCCCAGTCCAAGTAGAAAGCATGCTCAGATTTCCTAAGAGGC
AATCCTTCCCTCAAGGCAGCCTCATCAATTTGAATAACGTTAATGCCTGC
CTTCTCAAGATCCTCGACTTCGTCCTTGATGGCCAAAGCAATCTGGTAGC
AGGTTTCATGTCTGGGCTGGTCATTTCTAACAAAGGAC
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