CF832573

Overview
NameCF832573
Unique NameCF832573
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length788
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CF832573 vs. ExPASy Swiss-Prot
Match: METE_CAMJD (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Campylobacter jejuni subsp. doylei (strain ATCC BAA-1458 / RM4099 / 269.97) GN=metE PE=3 SV=1)

HSP 1 Score: 211.846 bits (538), Expect = 3.368e-54
Identity = 111/195 (56.92%), Postives = 138/195 (70.77%), Query Frame = -2
Query:  206 SFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSE-HAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAKLLRTQL 787
            SF R D    E+  QIALAI+DEV DLE AGI +IQIDEAALRE LPLRKS+ H+ YLDWA+ +F + + GV+  TQIHTHMCYS F+DI+  I  MDADVI+ E SRS+  LL   +  V +   +GPGVYDIHSPR+PS EE++  I K+L  L    +W+NPDCGLKTR Y EV  +L N+V A + +R QL
Sbjct:  561 SFPREDISLKESTEQIALAIRDEVLDLENAGIKIIQIDEAALREKLPLRKSDWHSEYLDWAIPAFNLVHSGVKAKTQIHTHMCYSEFSDILKEIDAMDADVISFEASRSNLSLLDTLK-AVHFKTEVGPGVYDIHSPRVPSVEELSLTIEKILNKLPKEQIWINPDCGLKTRAYEEVIASLKNLVTATQKIRKQL 754          
BLAST of CF832573 vs. ExPASy Swiss-Prot
Match: METE_DESVM (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Desulfovibrio vulgaris (strain Miyazaki F / DSM 19637) GN=metE PE=3 SV=1)

HSP 1 Score: 211.075 bits (536), Expect = 5.746e-54
Identity = 109/191 (57.07%), Postives = 138/191 (72.25%), Query Frame = -2
Query:  215 SFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAKLLR 787
            SFVR+D PR     Q+ALAI+DEV DLE AGI VIQIDE ALREGLPLR++ HA YLD AV +FR+ + GV+D TQIHTHMCY +F+DII  +  +DADVI++E SRS  +LL VF     Y   +GPGVYDIHSPR+PS +E+   +     VL  + LW NPDCGLKTR + E + +L+++VAAA  +R
Sbjct:  588 SFVRDDVPRPVVFRQLALAIRDEVADLEAAGIGVIQIDEPALREGLPLRRAAHAAYLDAAVGAFRLASSGVRDATQIHTHMCYCDFHDIIDHVAALDADVISLEASRSRMELLDVFATHA-YPNEVGPGVYDIHSPRVPSIDEMETLLRAASRVLPLDRLWANPDCGLKTRDWPETRASLAHLVAAATRVR 777          
BLAST of CF832573 vs. ExPASy Swiss-Prot
Match: METE_SHISS (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Shigella sonnei (strain Ss046) GN=metE PE=3 SV=1)

HSP 1 Score: 210.69 bits (535), Expect = 7.504e-54
Identity = 112/191 (58.64%), Postives = 129/191 (67.54%), Query Frame = -2
Query:  215 SFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAKLLR 787
            SF R D  R     QIALA++DEV DLE AGI +IQIDE ALREGLPLR+S+   YL W V +FRI     +D TQIHTHMCY  FNDI+ SI  +DADVITIE SRSD +LL  F E   Y   IGPGVYDIHSP +PS E I   + K    +    LWVNPDCGLKTR + E + AL+NMV AA+ LR
Sbjct:  562 SFPREDVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEAFRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEE-FDYPNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAALANMVQAAQNLR 751          
BLAST of CF832573 vs. ExPASy Swiss-Prot
Match: METE_SHIFL (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Shigella flexneri GN=metE PE=3 SV=1)

HSP 1 Score: 210.69 bits (535), Expect = 7.504e-54
Identity = 112/191 (58.64%), Postives = 129/191 (67.54%), Query Frame = -2
Query:  215 SFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAKLLR 787
            SF R D  R     QIALA++DEV DLE AGI +IQIDE ALREGLPLR+S+   YL W V +FRI     +D TQIHTHMCY  FNDI+ SI  +DADVITIE SRSD +LL  F E   Y   IGPGVYDIHSP +PS E I   + K    +    LWVNPDCGLKTR + E + AL+NMV AA+ LR
Sbjct:  562 SFPREDVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEAFRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEE-FDYPNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAALANMVQAAQNLR 751          
BLAST of CF832573 vs. ExPASy Swiss-Prot
Match: METE_SHIF8 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Shigella flexneri serotype 5b (strain 8401) GN=metE PE=3 SV=1)

HSP 1 Score: 210.69 bits (535), Expect = 7.504e-54
Identity = 112/191 (58.64%), Postives = 129/191 (67.54%), Query Frame = -2
Query:  215 SFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAKLLR 787
            SF R D  R     QIALA++DEV DLE AGI +IQIDE ALREGLPLR+S+   YL W V +FRI     +D TQIHTHMCY  FNDI+ SI  +DADVITIE SRSD +LL  F E   Y   IGPGVYDIHSP +PS E I   + K    +    LWVNPDCGLKTR + E + AL+NMV AA+ LR
Sbjct:  562 SFPREDVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEAFRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEE-FDYPNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAALANMVQAAQNLR 751          
BLAST of CF832573 vs. ExPASy Swiss-Prot
Match: METE_SHIDS (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Shigella dysenteriae serotype 1 (strain Sd197) GN=metE PE=3 SV=1)

HSP 1 Score: 210.69 bits (535), Expect = 7.504e-54
Identity = 112/191 (58.64%), Postives = 129/191 (67.54%), Query Frame = -2
Query:  215 SFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAKLLR 787
            SF R D  R     QIALA++DEV DLE AGI +IQIDE ALREGLPLR+S+   YL W V +FRI     +D TQIHTHMCY  FNDI+ SI  +DADVITIE SRSD +LL  F E   Y   IGPGVYDIHSP +PS E I   + K    +    LWVNPDCGLKTR + E + AL+NMV AA+ LR
Sbjct:  562 SFPREDVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEAFRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEE-FDYPNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAALANMVQAAQNLR 751          
BLAST of CF832573 vs. ExPASy Swiss-Prot
Match: METE_SHIBS (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Shigella boydii serotype 4 (strain Sb227) GN=metE PE=3 SV=1)

HSP 1 Score: 210.69 bits (535), Expect = 7.504e-54
Identity = 112/191 (58.64%), Postives = 129/191 (67.54%), Query Frame = -2
Query:  215 SFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAKLLR 787
            SF R D  R     QIALA++DEV DLE AGI +IQIDE ALREGLPLR+S+   YL W V +FRI     +D TQIHTHMCY  FNDI+ SI  +DADVITIE SRSD +LL  F E   Y   IGPGVYDIHSP +PS E I   + K    +    LWVNPDCGLKTR + E + AL+NMV AA+ LR
Sbjct:  562 SFPREDVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEAFRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEE-FDYPNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAALANMVQAAQNLR 751          
BLAST of CF832573 vs. ExPASy Swiss-Prot
Match: METE_SHIB3 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Shigella boydii serotype 18 (strain CDC 3083-94 / BS512) GN=metE PE=3 SV=1)

HSP 1 Score: 210.69 bits (535), Expect = 7.504e-54
Identity = 112/191 (58.64%), Postives = 129/191 (67.54%), Query Frame = -2
Query:  215 SFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAKLLR 787
            SF R D  R     QIALA++DEV DLE AGI +IQIDE ALREGLPLR+S+   YL W V +FRI     +D TQIHTHMCY  FNDI+ SI  +DADVITIE SRSD +LL  F E   Y   IGPGVYDIHSP +PS E I   + K    +    LWVNPDCGLKTR + E + AL+NMV AA+ LR
Sbjct:  562 SFPREDVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEAFRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEE-FDYPNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAALANMVQAAQNLR 751          
BLAST of CF832573 vs. ExPASy Swiss-Prot
Match: METE_SALTY (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Salmonella typhimurium GN=metE PE=3 SV=3)

HSP 1 Score: 210.69 bits (535), Expect = 7.504e-54
Identity = 112/191 (58.64%), Postives = 129/191 (67.54%), Query Frame = -2
Query:  215 SFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAKLLR 787
            SF R D  R     QIALA++DEV DLE AGI +IQIDE ALREGLPLR+S+   YL+W V +FRI     +D TQIHTHMCY  FNDI+ SI  +DADVITIE SRSD +LL  F E   Y   IGPGVYDIHSP +PS E I   + K    +    LWVNPDCGLKTR + E + AL+NMV AA  LR
Sbjct:  562 SFPREDVTRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLEWGVEAFRINAAVAKDETQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESF-EAFDYPNEIGPGVYDIHSPNVPSVEWIEALLKKAAQRIPAQRLWVNPDCGLKTRGWPETRAALANMVKAAHNLR 751          
BLAST of CF832573 vs. ExPASy Swiss-Prot
Match: METE_SALTI (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Salmonella typhi GN=metE PE=3 SV=3)

HSP 1 Score: 210.69 bits (535), Expect = 7.504e-54
Identity = 112/191 (58.64%), Postives = 129/191 (67.54%), Query Frame = -2
Query:  215 SFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAKLLR 787
            SF R D  R     QIALA++DEV DLE AGI +IQIDE ALREGLPLR+S+   YL+W V +FRI     +D TQIHTHMCY  FNDI+ SI  +DADVITIE SRSD +LL  F E   Y   IGPGVYDIHSP +PS E I   + K    +    LWVNPDCGLKTR + E + AL+NMV AA  LR
Sbjct:  562 SFPREDVTRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLEWGVEAFRINAAVAKDETQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESF-EAFDYPNEIGPGVYDIHSPNVPSVEWIEALLKKAAQRIPAQRLWVNPDCGLKTRGWPETRAALANMVKAAHNLR 751          
The following BLAST results are available for this feature:
BLAST of CF832573 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 283
Match NameE-valueIdentityDescription
METE_CAMJD3.368e-5456.925-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_DESVM5.746e-5457.075-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_SHISS7.504e-5458.645-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_SHIFL7.504e-5458.645-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_SHIF87.504e-5458.645-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_SHIDS7.504e-5458.645-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_SHIBS7.504e-5458.645-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_SHIB37.504e-5458.645-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_SALTY7.504e-5458.645-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_SALTI7.504e-5458.645-methyltetrahydropteroyltriglutamate--homocystein... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS02_02B06_f Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa02B06, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF832573 ID=CF832573; Name=CF832573; organism=Citrus sinensis; type=EST; length=788bp
TTTTTTTTTTTTTTTAGAAAATAGAAGCCAATTGCTCAATAGTGCATAAT
ATGCATACGGAAAATTTTCATCACATCACGACCCACAGTTATTCGAATAG
GTAATAAGACAGGAATTTTCTTTCTTTGTTTAAAGTGAAAAAAAAAAAAA
TCAATGGCTCAGGAAGTATCCCTCCAATCTCCAGAACCACTCACTTGGCA
CTGGCAAGCTGGGTGCGGAGTAGCTTGGCAGCAGCGACCATGTTTGAGAG
GGCTGGCTTCACCTCAGTGTACTTGCGAGTCTTAAGACCACAGTCAGGGT
TAACCCACAAAATGTTGGTTTCTAGCACAGCAAGCATCTTGTTGATTCTG
TCAGCAATTTCTTCAGTTGATGGTATTCTTGGAGAGTGGATGTCATACAC
ACCAGGGCCAATTCCAGCACCATACTTGACTCCCTCACGGAAGACAGACA
GGAGCTTCTCATCAGAACGAGAGTTCTCAATGGTGATCACATCAGCATCC
ATGTCGATAATTGAGTGGATAATGTCGTTGAAGTTGGAGTAGCACATGTG
GGTGTGGATCTGGGTAGTGTCCTGGACGCCACAGTTGGTGATTCTGAAGG
AGTGGACTGCCCAGTCCAAGTAGAAAGCATGCTCAGATTTCCTAAGAGGC
AATCCTTCCCTCAAGGCAGCCTCATCAATTTGAATAACGTTAATGCCTGC
CTTCTCAAGATCCTCGACTTCGTCCTTGATGGCCAAAGCAATCTGGTAGC
AGGTTTCATGTCTGGGCTGGTCATTTCTAACAAAGGAC
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