CF832574

Overview
NameCF832574
Unique NameCF832574
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length716
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig9752 contig Csv1_Contig9752:92..807. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CF832574 vs. ExPASy Swiss-Prot
Match: METE_BURA4 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Burkholderia ambifaria (strain MC40-6) GN=metE PE=3 SV=1)

HSP 1 Score: 334.339 bits (856), Expect = 3.812e-91
Identity = 166/238 (69.75%), Postives = 191/238 (80.25%), Query Frame = 1 
Query:    1 YGSPCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAK 714
            YGS CVKPPI++GD+SRPKAMTV W + AQS+T +PMKGMLTGPVTILNWSFVR+DQPR  +CYQ+ALAI+DEV DLEKAG+ VIQIDEAALREGLPLR+++   YL WAV +FRIT  GVQD TQIHTHMCYS FNDII SI DMDADVITIE SRSD +LL  F +  +Y   IGPGVYDIHSP IP+ + I   + K    +    LWVNPDCGLKTR++ EV PAL+NMVAAAK
Sbjct:  521 YGSRCVKPPILFGDISRPKAMTVEWITYAQSLTNKPMKGMLTGPVTILNWSFVRDDQPRSVSCYQLALAIRDEVLDLEKAGVRVIQIDEAALREGLPLRRAQWGEYLKWAVEAFRITANGVQDDTQIHTHMCYSEFNDIIASIADMDADVITIETSRSDMELLDAF-DSFRYPNEIGPGVYDIHSPNIPTQDHIVGLMKKAAERIPAERLWVNPDCGLKTRQWAEVIPALTNMVAAAK 757
[Close]
BLAST of CF832574 vs. ExPASy Swiss-Prot
Match: METE_BURVG (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Burkholderia vietnamiensis (strain G4 / LMG 22486) GN=metE PE=3 SV=1)

HSP 1 Score: 330.872 bits (847), Expect = 4.215e-90
Identity = 162/238 (68.07%), Postives = 191/238 (80.25%), Query Frame = 1 
Query:    1 YGSPCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAK 714
            YGS CVKPPI++GD+SRP AMTV W + AQS+T++PMKGMLTGPVTILNWSFVR+DQPR  +CYQ+ALAI+DEV DLE+AG+ VIQIDEAALREGLPLR+++   YL WA+ +FRI   GV+D TQIHTHMCYS FNDII SI +MDADVITIE SRSD +LL  F +G +Y   IGPGVYDIHSP IP+ E I   + K    +    LWVNPDCGLKTR++ EV PAL+NMVAAAK
Sbjct:  521 YGSRCVKPPILFGDISRPSAMTVEWIAYAQSLTSKPMKGMLTGPVTILNWSFVRDDQPRSVSCYQLALAIRDEVLDLERAGVRVIQIDEAALREGLPLRRAQWPAYLKWAIDAFRIAANGVRDDTQIHTHMCYSEFNDIIASIAEMDADVITIETSRSDMELLDAF-DGFRYPNEIGPGVYDIHSPNIPTQEHIVGLMRKAAERIPAQRLWVNPDCGLKTRRWAEVLPALTNMVAAAK 757
[Close]
BLAST of CF832574 vs. ExPASy Swiss-Prot
Match: METE_FLAJ1 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Flavobacterium johnsoniae (strain ATCC 17061 / DSM 2064 / UW101) GN=metE PE=3 SV=1)

HSP 1 Score: 326.635 bits (836), Expect = 7.948e-89
Identity = 164/238 (68.91%), Postives = 186/238 (78.15%), Query Frame = 1 
Query:    1 YGSPCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAK 714
            YGS CVKPP+IYGDVSRP  MTV WS  AQS+T++ +KGMLTGPVTIL WSFVRNDQPR ETC QIALA++DEV DLEKAGI +IQIDE A+REGLPLRK E A YLDWAV +FRI+  GV D TQIH HMCYS FNDII +I DMDADVITIE SRS  +LL  F    KY   IGPGVYDIHSPR+PS++E+   + K  AV+  + LWVNPDCGLKTR + E K AL  MVAAA+
Sbjct:  526 YGSRCVKPPVIYGDVSRPNPMTVKWSKYAQSLTSKWVKGMLTGPVTILQWSFVRNDQPRSETCTQIALAVRDEVVDLEKAGIKIIQIDEPAIREGLPLRKEEWAKYLDWAVKAFRISASGVNDDTQIHPHMCYSEFNDIIQNIADMDADVITIECSRSQMELLDAF-ANFKYPNEIGPGVYDIHSPRVPSSKEMVRLLEKASAVIPVDQLWVNPDCGLKTRHWNETKKALIEMVAAAQ 762
[Close]
BLAST of CF832574 vs. ExPASy Swiss-Prot
Match: METE_HAHCH (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Hahella chejuensis (strain KCTC 2396) GN=metE PE=3 SV=1)

HSP 1 Score: 326.25 bits (835), Expect = 1.038e-88
Identity = 163/238 (68.49%), Postives = 185/238 (77.73%), Query Frame = 1 
Query:    1 YGSPCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAK 714
            YGS CVKPPII+GD+SRP+AMTV W   AQS+T RPMKGMLTGPVTILNWSFVR+DQPR  TCYQ+ALAI+ EV+DLE+AG+ +IQIDEAALREGLPLR+++   YL WAV SFRI   GV D TQIHTHMCYS FNDII +I  MDADVITIE SRSD +LL  F +  KY   IGPGVYDIHSP IPS   I   + K    +  + LWVNPDCGLKTR + EV+PAL NMV AAK
Sbjct:  516 YGSRCVKPPIIFGDISRPQAMTVEWIKYAQSLTDRPMKGMLTGPVTILNWSFVRDDQPRALTCYQLALAIRAEVQDLERAGVRIIQIDEAALREGLPLRRAQWREYLQWAVSSFRIAANGVADETQIHTHMCYSEFNDIIEAIAGMDADVITIETSRSDMELLDAF-DNFKYPNEIGPGVYDIHSPNIPSETHIVQLMRKAAERIPAHRLWVNPDCGLKTRNWEEVRPALQNMVNAAK 752
[Close]
BLAST of CF832574 vs. ExPASy Swiss-Prot
Match: METE_PSYIN (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Psychromonas ingrahamii (strain 37) GN=metE PE=3 SV=1)

HSP 1 Score: 325.865 bits (834), Expect = 1.356e-88
Identity = 164/238 (68.91%), Postives = 186/238 (78.15%), Query Frame = 1 
Query:    1 YGSPCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAK 714
            YGS CVKPPI++GDVSRPKAMTV WSS AQS+T + +KGMLTGPVTILNWSFVR+DQP   TC QIALAI++EV DLEKAGIN+IQIDEAALRE LPL +S+   YL+WA+ +FR+   GV D TQIHTHMCYS FNDII +I  MDADVITIE SRSD  LL VF E   Y  GIGPGVYDIHSP IP  E++   + K    +    LWVNPDCGLKTR++ EVKPAL NMVAAAK
Sbjct:  526 YGSRCVKPPILFGDVSRPKAMTVEWSSYAQSLTEKKVKGMLTGPVTILNWSFVRDDQPLQTTCMQIALAIREEVLDLEKAGINIIQIDEAALREKLPLHRSQWQQYLNWAIAAFRLCANGVNDKTQIHTHMCYSEFNDIIAAIAQMDADVITIETSRSDMVLLDVF-EAFSYPNGIGPGVYDIHSPNIPDVEQMVSLMKKAALRIPAEQLWVNPDCGLKTRRWEEVKPALLNMVAAAK 762
[Close]
BLAST of CF832574 vs. ExPASy Swiss-Prot
Match: METE_MARAV (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Marinobacter aquaeolei (strain ATCC 700491 / DSM 11845 / VT8) GN=metE PE=3 SV=1)

HSP 1 Score: 325.094 bits (832), Expect = 2.313e-88
Identity = 158/238 (66.39%), Postives = 189/238 (79.41%), Query Frame = 1 
Query:    1 YGSPCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAK 714
            YGS CVKPPI++GD+ RP+AMTV W   AQS+T +P+KGMLTGPVTILNWSFVR+DQPR ++C Q+ALAI++EV+DLEKAG+N+IQIDEAALREGLPLR+S+ A YL+WA++SFRI   GV D+TQIHTHMCYS FNDII +I  MDADVITIE SRSD +LL  FR   +Y   IGPGVYDIHSP IP  E I + +      +    LW+NPDCGLKTRK+ EV PAL  MVAAA+
Sbjct:  516 YGSRCVKPPILFGDIRRPQAMTVEWIRYAQSLTDKPVKGMLTGPVTILNWSFVRDDQPRKDSCLQLALAIREEVQDLEKAGVNIIQIDEAALREGLPLRQSDWATYLEWAINSFRIAANGVDDSTQIHTHMCYSEFNDIIEAIARMDADVITIETSRSDMELLDAFR-SFEYPNDIGPGVYDIHSPNIPDKEHIINLMTLAAERIPAERLWINPDCGLKTRKWEEVTPALETMVAAAR 752
[Close]
BLAST of CF832574 vs. ExPASy Swiss-Prot
Match: METE_BURP8 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Burkholderia phymatum (strain DSM 17167 / STM815) GN=metE PE=3 SV=1)

HSP 1 Score: 323.939 bits (829), Expect = 5.152e-88
Identity = 163/238 (68.49%), Postives = 184/238 (77.31%), Query Frame = 1 
Query:    1 YGSPCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAK 714
            YGS CVKPPI++GD+SRPKAMTV W   AQ+ TA+PMKGMLTGPVTILNWSFVR+DQPR  +C Q+ALAI++EV DLEKAG+ VIQIDEAALREGLPLR+S+   YL WAV SFRI   GVQD TQIHTHMCYS FNDII SI +MDADVITIE SRSD +LL  F +   Y   IGPGVYDIHSP IP    + D + K    +    LWVNPDCGLKTR + EV PAL NMVAAA+
Sbjct:  521 YGSRCVKPPILFGDISRPKAMTVEWICYAQAQTAKPMKGMLTGPVTILNWSFVRDDQPRSVSCRQLALAIREEVLDLEKAGVRVIQIDEAALREGLPLRRSQWNEYLQWAVESFRIAANGVQDETQIHTHMCYSEFNDIIASIAEMDADVITIETSRSDMELLDAF-DDFHYPNQIGPGVYDIHSPNIPDQAHVVDLMKKAAERIPAERLWVNPDCGLKTRAWEEVIPALKNMVAAAR 757
[Close]
BLAST of CF832574 vs. ExPASy Swiss-Prot
Match: METE_THIDA (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Thiobacillus denitrificans (strain ATCC 25259) GN=metE PE=3 SV=1)

HSP 1 Score: 319.316 bits (817), Expect = 1.269e-86
Identity = 158/238 (66.39%), Postives = 183/238 (76.89%), Query Frame = 1 
Query:    1 YGSPCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAK 714
            YGS CVKPPI++GDV RP+ MTV W+   QS+T +PMKGMLTGPVT+LNWSFVR+DQPR E+C Q+AL I+DEV DLEKAG+ VIQIDEAALREGLPLR+S+   YL WAV SFRI   GV D TQIHTHMCYS FNDII +I  MDADVITIE SRSD +LL  F +   Y   IGPGVYDIHSP IP+ + I + I K    +    LW+NPDCGLKTR++ EV PAL NMVAAA+
Sbjct:  523 YGSRCVKPPILFGDVRRPRPMTVEWTRYGQSLTEKPMKGMLTGPVTLLNWSFVRDDQPRAESCKQLALTIRDEVADLEKAGVRVIQIDEAALREGLPLRRSQWKTYLGWAVESFRIAANGVADETQIHTHMCYSEFNDIIEAIAAMDADVITIETSRSDMELLDAF-DHFSYPNEIGPGVYDIHSPNIPTPQHIVNLIKKAAERIPAERLWINPDCGLKTRQWNEVIPALGNMVAAAR 759
[Close]
BLAST of CF832574 vs. ExPASy Swiss-Prot
Match: METE_CYTH3 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Cytophaga hutchinsonii (strain ATCC 33406 / NCIMB 9469) GN=metE PE=3 SV=1)

HSP 1 Score: 319.316 bits (817), Expect = 1.269e-86
Identity = 158/238 (66.39%), Postives = 186/238 (78.15%), Query Frame = 1 
Query:    1 YGSPCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAK 714
            YGS CVKPPIIYGD+ R + MTV W++LAQS+T + MKGMLTGPVTIL WSFVR+DQPR +TC QIA AI+DEV DLE AGI +IQIDE A+REGLPLR+     YL+WAV  FRI+  GV+D TQIHTHMCYS FNDII +I  MDADVITIE SRS  +LL  F +   Y   IGPGVYDIHSPRIP+T+E+AD +NK + VL    +WVNPDCGLKTR++ E + AL NMVAAA+
Sbjct:  529 YGSRCVKPPIIYGDIERTEPMTVAWTTLAQSLTKKYMKGMLTGPVTILQWSFVRDDQPRRDTCMQIAFAIRDEVVDLEAAGIKIIQIDEPAIREGLPLRRENWETYLNWAVECFRISASGVKDETQIHTHMCYSEFNDIIKNIAAMDADVITIETSRSQMELLDAFVD-FNYPNEIGPGVYDIHSPRIPTTDEMADLLNKAMHVLPVRNIWVNPDCGLKTRRWPETEAALINMVAAAQ 765
[Close]
BLAST of CF832574 vs. ExPASy Swiss-Prot
Match: METE_ALHEH (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Alkalilimnicola ehrlichei (strain MLHE-1) GN=metE PE=3 SV=1)

HSP 1 Score: 318.546 bits (815), Expect = 2.165e-86
Identity = 160/238 (67.23%), Postives = 182/238 (76.47%), Query Frame = 1 
Query:    1 YGSPCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAK 714
            YGS CVKPPIIYGDVSRP+ MTV WS  AQS+T RPMKGMLTGPVT+L WSFVR+DQPR ETC QIALA++DEV DLE AGI  IQIDE ALREGLPL++ +   YLDWAV  FR+T    QD TQIHTHMCYS FNDI+ +I  +DADVITIE SRSD +LL VF EG +Y   IGPGVYDIHSP  PS + +   + K    +    LWVNPDCGLKTR + EV+PAL NMVAAA+
Sbjct:  518 YGSRCVKPPIIYGDVSRPRPMTVRWSEYAQSLTGRPMKGMLTGPVTVLQWSFVRDDQPRSETCRQIALALRDEVADLEAAGIPAIQIDEPALREGLPLQQRQWPGYLDWAVDCFRLTASVAQDATQIHTHMCYSEFNDIMGAIATLDADVITIETSRSDMELLEVF-EGFEYPNEIGPGVYDIHSPNTPSVDWMVRLMEKAARRIPVERLWVNPDCGLKTRAWAEVEPALQNMVAAAE 754
The following BLAST results are available for this feature:
BLAST of CF832574 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 286
Match NameE-valueIdentityDescription
METE_BURA43.812e-9169.755-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_BURVG4.215e-9068.075-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_FLAJ17.948e-8968.915-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_HAHCH1.038e-8868.495-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_PSYIN1.356e-8868.915-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_MARAV2.313e-8866.395-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_BURP85.152e-8868.495-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_THIDA1.269e-8666.395-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_CYTH31.269e-8666.395-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_ALHEH2.165e-8667.235-methyltetrahydropteroyltriglutamate--homocystein... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS02_02B06_r Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa02B06, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF832574 ID=CF832574; Name=CF832574; organism=Citrus sinensis; type=EST; length=716bp
TATGGATCTCCCTGTGTGAAGCCACCCATCATCTATGGTGATGTGAGCCG
TCCCAAGGCTATGACCGTCTTCTGGTCCTCCTTGGCTCAGAGCATGACAG
CTCGCCCAATGAAAGGAATGCTTACTGGCCCTGTCACCATTCTCAACTGG
TCCTTTGTTAGAAATGACCAGCCCAGACATGAAACCTGCTACCAGATTGC
TTTGGCCATCAAGGACGAAGTCGAGGATCTTGAGAAGGCAGGCATTAACG
TTATTCAAATTGATGAGGCTGCCTTGAGGGAAGGATTGCCTCTTAGGAAA
TCTGAGCATGCTTTCTACTTGGACTGGGCAGTCCACTCCTTCAGAATCAC
CAACTGTGGCGTCCAGGACACTACCCAGATCCACACCCACATGTGCTACT
CCAACTTCAACGACATTATCCACTCAATTATCGACATGGATGCTGATGTG
ATCACCATTGAGAACTCTCGTTCTGATGAGAAGCTCCTGTCTGTCTTCCG
TGAGGGAGTCAAGTATGGTGCTGGAATTGGCCCTGGTGTGTATGACATCC
ACTCTCCAAGAATACCATCAACTGAAGAAATTGCTGACAGAATCAACAAG
ATGCTTGCTGTGCTAGAAACCAACATTTTGTGGGTTAACCCTGACTGTGG
TCTTAAGACTCGCAAGTACACTGAGGTGAAGCCAGCCCTCTCAAACATGG
TCGCTGCTGCCAAGCT
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