CF832574

Overview
NameCF832574
Unique NameCF832574
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length716
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig9752 contig Csv1_Contig9752:92..807. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CF832574 vs. ExPASy Swiss-Prot
Match: METE_SACD2 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024) GN=metE PE=3 SV=1)

HSP 1 Score: 317.39 bits (812), Expect = 4.822e-86
Identity = 155/238 (65.13%), Postives = 185/238 (77.73%), Query Frame = 1 
Query:    1 YGSPCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAK 714
            YGS CVKPPII+GDVSRP+ +TV W+  AQS+T++P+KGMLTGP+T+L WSFVR+DQPR  T  QIALA++ EV DLE AGI +IQ+DE ALREGLPLRK +   YLDWAV +F+I+  GV DTTQIHTHMCY+ FNDII +I D+DADVITIE SRS+ +LLS F E   Y   IGPGVYDIHSP IP+TE++   I K    L    LW+NPDCGLKTRK+ +V PAL NMV AAK
Sbjct:  522 YGSRCVKPPIIFGDVSRPRKITVDWAEYAQSLTSKPVKGMLTGPITMLQWSFVRDDQPRKITAQQIALALRSEVNDLEAAGITIIQVDEPALREGLPLRKKDWQNYLDWAVEAFKISTAGVGDTTQIHTHMCYAEFNDIIQAIADLDADVITIETSRSNMELLSAF-EQFNYPNDIGPGVYDIHSPNIPATEQVVGLIKKAAENLPLQRLWINPDCGLKTRKWEDVIPALENMVNAAK 758
[Close]
BLAST of CF832574 vs. ExPASy Swiss-Prot
Match: METE_RUTMC (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Ruthia magnifica subsp. Calyptogena magnifica GN=metE PE=3 SV=1)

HSP 1 Score: 315.849 bits (808), Expect = 1.403e-85
Identity = 154/238 (64.71%), Postives = 185/238 (77.73%), Query Frame = 1 
Query:    1 YGSPCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAK 714
            YGS CVKPPII+GD+ RPK MT+ W + AQS+T +PMKGM+TGPVT+LNWSFVR+DQPR  TC Q++LAI+DEV DLEK+ IN+IQIDEAALREGLPLRKS+   YLDWA+ ++R++  GV D TQIHTHMCYS FNDII +I  MDADVITIE SRSD +LL +F E   Y   IGPGVYDIHSP IPS + I + + K    +    LWVNPDCGLKTR + EV  AL+NMV A++
Sbjct:  517 YGSRCVKPPIIFGDIFRPKPMTLDWITYAQSLTNKPMKGMITGPVTMLNWSFVRDDQPRATTCLQLSLAIRDEVLDLEKSNINIIQIDEAALREGLPLRKSQWQTYLDWAIRAYRVSANGVSDETQIHTHMCYSEFNDIIEAIAQMDADVITIETSRSDMELLDIFDE-FDYPNEIGPGVYDIHSPNIPSVDSIVELMQKAAKYIPIKRLWVNPDCGLKTRHWDEVNLALTNMVLASQ 753
[Close]
BLAST of CF832574 vs. ExPASy Swiss-Prot
Match: METE_NITWN (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Nitrobacter winogradskyi (strain Nb-255 / ATCC 25391) GN=metE PE=3 SV=1)

HSP 1 Score: 315.464 bits (807), Expect = 1.832e-85
Identity = 156/238 (65.55%), Postives = 182/238 (76.47%), Query Frame = 1 
Query:    1 YGSPCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAK 714
            YGS CV+PP+++GDVSRP+ MTV W   AQS+TA+PMKGMLTGPVTILNWSFVR+D PR   C QIALAI+DEV DLE+AG  +IQIDEAALREGLPLR+SE   YLDWAV  FR+   GV D TQIHTHMCYS FNDII +I  MDADVI+IE SRS  +LL  F+   KY   IGPGVYDIHSPR+P+ +E+   +      L    +W+NPDCGLKTRK+ EV+PAL NMV AAK
Sbjct:  532 YGSRCVRPPVLFGDVSRPRPMTVGWWKYAQSLTAKPMKGMLTGPVTILNWSFVRDDVPRSLACRQIALAIRDEVSDLEQAGATMIQIDEAALREGLPLRRSEWKTYLDWAVECFRLCASGVADATQIHTHMCYSEFNDIIDAIASMDADVISIETSRSKMELLDAFKT-YKYPNEIGPGVYDIHSPRVPAVDEMTGLLKLARERLSDRQIWINPDCGLKTRKWEEVRPALVNMVEAAK 768
[Close]
BLAST of CF832574 vs. ExPASy Swiss-Prot
Match: METE_DICDI (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Dictyostelium discoideum GN=metE PE=3 SV=1)

HSP 1 Score: 314.309 bits (804), Expect = 4.082e-85
Identity = 154/238 (64.71%), Postives = 186/238 (78.15%), Query Frame = 1 
Query:    1 YGSPCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAK 714
            YGS CVKPPIIYGDV+RP  MT+ +++ AQ++T +PMKGMLTGPVTIL WSFVR+DQPR ETC+QI LAI+DEV DLE  GI  IQIDE A+REGLPLR S+   YL WA+ SF +++ GV+D+TQIH+HMCYS+FNDI  SI  MD DV+TIENS+SD KLL  F E   Y   IGPG+YDIHSPRIPS E++ DR+ +ML  L TN+LW+NPDCGLKTR+    + AL NMV  AK
Sbjct:  579 YGSRCVKPPIIYGDVNRPVPMTLEYTTFAQTLTTKPMKGMLTGPVTILQWSFVRDDQPRSETCFQIGLAIRDEVTDLENKGIACIQIDEPAIREGLPLRLSDWNQYLKWAIDSFLLSSTGVKDSTQIHSHMCYSDFNDIFESIQRMDTDVLTIENSKSDLKLLKAF-EKYGYTNEIGPGLYDIHSPRIPSVEDMKDRVEQMLKYLSTNLLWINPDCGLKTREPETTRLALINMVKVAK 815
[Close]
BLAST of CF832574 vs. ExPASy Swiss-Prot
Match: METE_XYLFA (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Xylella fastidiosa GN=metE PE=3 SV=1)

HSP 1 Score: 313.923 bits (803), Expect = 5.331e-85
Identity = 157/238 (65.97%), Postives = 184/238 (77.31%), Query Frame = 1 
Query:    1 YGSPCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAK 714
            YGS CVKPPIIYGDV RP  MTV WS+ AQS+T +PMKGMLTGPVT+L WSFVR+DQ R +TC QIALA++DEV+DLEKAGI VIQIDE A+REGLPLR+ E A YL+WAV SFRI +  V+DTTQIHTHMCYS FNDII ++  +DADVI+IE SRS  +LL  F +  +Y   IGPGVYDIHSPR+P  EE+   + K  AVL    LWVNPDCGLKTR + E + AL  MV AA+
Sbjct:  517 YGSRCVKPPIIYGDVVRPAPMTVTWSAYAQSLTDKPMKGMLTGPVTMLQWSFVRDDQERAQTCRQIALALRDEVQDLEKAGIKVIQIDEPAIREGLPLRRGEWADYLNWAVESFRIASSNVRDTTQIHTHMCYSEFNDIIEAVAALDADVISIETSRSRMELLDAFVK-FRYPNAIGPGVYDIHSPRVPQEEEMVLLLKKARAVLPPEQLWVNPDCGLKTRGWKETRAALQTMVHAAQ 753
[Close]
BLAST of CF832574 vs. ExPASy Swiss-Prot
Match: METE_XYLFT (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Xylella fastidiosa (strain Temecula1 / ATCC 700964) GN=metE PE=3 SV=1)

HSP 1 Score: 313.538 bits (802), Expect = 6.963e-85
Identity = 157/238 (65.97%), Postives = 183/238 (76.89%), Query Frame = 1 
Query:    1 YGSPCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAK 714
            YGS CVKPPIIYGDV RP  MTV WS+ AQS+T +PMKGMLTGPVT+L WSFVR+DQ R +TC QIALA++DEV+DLEKAGI VIQIDE A+REGLPLR+ E A YL+WAV SFRI +  V DTTQIHTHMCYS FNDII ++  +DADVI+IE SRS  +LL  F +  +Y   IGPGVYDIHSPR+P  EE+   + K  AVL    LWVNPDCGLKTR + E + AL  MV AA+
Sbjct:  517 YGSRCVKPPIIYGDVVRPAPMTVTWSAYAQSLTDKPMKGMLTGPVTMLQWSFVRDDQERAQTCRQIALALRDEVQDLEKAGIKVIQIDEPAIREGLPLRRGEWADYLNWAVESFRIASSNVHDTTQIHTHMCYSEFNDIIEAVAALDADVISIETSRSRMELLDAFVK-FRYPNAIGPGVYDIHSPRVPQEEEMVLLLKKARAVLPPEQLWVNPDCGLKTRGWKETRAALQTMVHAAQ 753
[Close]
BLAST of CF832574 vs. ExPASy Swiss-Prot
Match: METE_XYLFM (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Xylella fastidiosa (strain M12) GN=metE PE=3 SV=1)

HSP 1 Score: 313.538 bits (802), Expect = 6.963e-85
Identity = 157/238 (65.97%), Postives = 183/238 (76.89%), Query Frame = 1 
Query:    1 YGSPCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAK 714
            YGS CVKPPIIYGDV RP  MTV WS+ AQS+T +PMKGMLTGPVT+L WSFVR+DQ R +TC QIALA++DEV+DLEKAGI VIQIDE A+REGLPLR+ E A YL+WAV SFRI +  V DTTQIHTHMCYS FNDII ++  +DADVI+IE SRS  +LL  F +  +Y   IGPGVYDIHSPR+P  EE+   + K  AVL    LWVNPDCGLKTR + E + AL  MV AA+
Sbjct:  517 YGSRCVKPPIIYGDVVRPAPMTVTWSAYAQSLTDKPMKGMLTGPVTMLQWSFVRDDQERAQTCRQIALALRDEVQDLEKAGIKVIQIDEPAIREGLPLRRGEWADYLNWAVESFRIASSNVHDTTQIHTHMCYSEFNDIIEAVAALDADVISIETSRSRMELLDAFVK-FRYPNAIGPGVYDIHSPRVPQEEEMVLLLKKARAVLPPEQLWVNPDCGLKTRGWKETRAALQTMVHAAQ 753
[Close]
BLAST of CF832574 vs. ExPASy Swiss-Prot
Match: METE_XYLF2 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Xylella fastidiosa (strain M23) GN=metE PE=3 SV=1)

HSP 1 Score: 313.538 bits (802), Expect = 6.963e-85
Identity = 157/238 (65.97%), Postives = 183/238 (76.89%), Query Frame = 1 
Query:    1 YGSPCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAK 714
            YGS CVKPPIIYGDV RP  MTV WS+ AQS+T +PMKGMLTGPVT+L WSFVR+DQ R +TC QIALA++DEV+DLEKAGI VIQIDE A+REGLPLR+ E A YL+WAV SFRI +  V DTTQIHTHMCYS FNDII ++  +DADVI+IE SRS  +LL  F +  +Y   IGPGVYDIHSPR+P  EE+   + K  AVL    LWVNPDCGLKTR + E + AL  MV AA+
Sbjct:  517 YGSRCVKPPIIYGDVVRPAPMTVTWSAYAQSLTDKPMKGMLTGPVTMLQWSFVRDDQERAQTCRQIALALRDEVQDLEKAGIKVIQIDEPAIREGLPLRRGEWADYLNWAVESFRIASSNVHDTTQIHTHMCYSEFNDIIEAVAALDADVISIETSRSRMELLDAFVK-FRYPNAIGPGVYDIHSPRVPQEEEMVLLLKKARAVLPPEQLWVNPDCGLKTRGWKETRAALQTMVHAAQ 753
[Close]
BLAST of CF832574 vs. ExPASy Swiss-Prot
Match: METE_RHOPT (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Rhodopseudomonas palustris (strain TIE-1) GN=metE PE=3 SV=1)

HSP 1 Score: 313.153 bits (801), Expect = 9.094e-85
Identity = 159/238 (66.81%), Postives = 181/238 (76.05%), Query Frame = 1 
Query:    1 YGSPCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAK 714
            YGS CV+PPI++GDVSRPK MTV W   AQS+T RPMKGMLTGPVTILNWSFVR+D PR   C QIALAI+DEV DLE+AG  +IQIDEAALREGLPLR+SE   YLDWAV  FR+ + GV+D TQIHTHMCYS FNDII +I  MDADVI+IE SRS  +LL  F+   KY   IGPGVYDIHSPR+PS EE+   +      L    LWVNPDCGLKTR + EV+ AL NMV AA+
Sbjct:  544 YGSRCVRPPILFGDVSRPKPMTVGWWKYAQSLTGRPMKGMLTGPVTILNWSFVRDDVPRSTACLQIALAIRDEVGDLEQAGATMIQIDEAALREGLPLRRSEWKGYLDWAVECFRLCSSGVKDETQIHTHMCYSEFNDIIDAIAAMDADVISIETSRSKMELLDAFKT-YKYPNEIGPGVYDIHSPRVPSVEEMTTLLQLARQRLSDGQLWVNPDCGLKTRGWDEVQGALVNMVEAAR 780
[Close]
BLAST of CF832574 vs. ExPASy Swiss-Prot
Match: METE_RHOPA (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Rhodopseudomonas palustris GN=metE PE=3 SV=1)

HSP 1 Score: 313.153 bits (801), Expect = 9.094e-85
Identity = 159/238 (66.81%), Postives = 181/238 (76.05%), Query Frame = 1 
Query:    1 YGSPCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAK 714
            YGS CV+PPI++GDVSRPK MTV W   AQS+T RPMKGMLTGPVTILNWSFVR+D PR   C QIALAI+DEV DLE+AG  +IQIDEAALREGLPLR+SE   YLDWAV  FR+ + GV+D TQIHTHMCYS FNDII +I  MDADVI+IE SRS  +LL  F+   KY   IGPGVYDIHSPR+PS EE+   +      L    LWVNPDCGLKTR + EV+ AL NMV AA+
Sbjct:  544 YGSRCVRPPILFGDVSRPKPMTVGWWKYAQSLTGRPMKGMLTGPVTILNWSFVRDDVPRSTACLQIALAIRDEVGDLEQAGATMIQIDEAALREGLPLRRSEWKGYLDWAVECFRLCSSGVKDETQIHTHMCYSEFNDIIDAIAAMDADVISIETSRSKMELLDAFKT-YKYPNEIGPGVYDIHSPRVPSVEEMTTLLQLARQRLSDGQLWVNPDCGLKTRGWDEVQGALVNMVEAAR 780
The following BLAST results are available for this feature:
BLAST of CF832574 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 286
Match NameE-valueIdentityDescription
METE_SACD24.822e-8665.135-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_RUTMC1.403e-8564.715-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_NITWN1.832e-8565.555-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_DICDI4.082e-8564.715-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_XYLFA5.331e-8565.975-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_XYLFT6.963e-8565.975-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_XYLFM6.963e-8565.975-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_XYLF26.963e-8565.975-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_RHOPT9.094e-8566.815-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_RHOPA9.094e-8566.815-methyltetrahydropteroyltriglutamate--homocystein... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS02_02B06_r Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa02B06, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF832574 ID=CF832574; Name=CF832574; organism=Citrus sinensis; type=EST; length=716bp
TATGGATCTCCCTGTGTGAAGCCACCCATCATCTATGGTGATGTGAGCCG
TCCCAAGGCTATGACCGTCTTCTGGTCCTCCTTGGCTCAGAGCATGACAG
CTCGCCCAATGAAAGGAATGCTTACTGGCCCTGTCACCATTCTCAACTGG
TCCTTTGTTAGAAATGACCAGCCCAGACATGAAACCTGCTACCAGATTGC
TTTGGCCATCAAGGACGAAGTCGAGGATCTTGAGAAGGCAGGCATTAACG
TTATTCAAATTGATGAGGCTGCCTTGAGGGAAGGATTGCCTCTTAGGAAA
TCTGAGCATGCTTTCTACTTGGACTGGGCAGTCCACTCCTTCAGAATCAC
CAACTGTGGCGTCCAGGACACTACCCAGATCCACACCCACATGTGCTACT
CCAACTTCAACGACATTATCCACTCAATTATCGACATGGATGCTGATGTG
ATCACCATTGAGAACTCTCGTTCTGATGAGAAGCTCCTGTCTGTCTTCCG
TGAGGGAGTCAAGTATGGTGCTGGAATTGGCCCTGGTGTGTATGACATCC
ACTCTCCAAGAATACCATCAACTGAAGAAATTGCTGACAGAATCAACAAG
ATGCTTGCTGTGCTAGAAACCAACATTTTGTGGGTTAACCCTGACTGTGG
TCTTAAGACTCGCAAGTACACTGAGGTGAAGCCAGCCCTCTCAAACATGG
TCGCTGCTGCCAAGCT
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