CF832574

Overview
NameCF832574
Unique NameCF832574
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length716
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig9752 contig Csv1_Contig9752:92..807. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CF832574 vs. ExPASy Swiss-Prot
Match: METE_HAES1 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Haemophilus somnus (strain 129Pt) GN=metE PE=3 SV=1)

HSP 1 Score: 300.827 bits (769), Expect = 4.671e-81
Identity = 150/238 (63.03%), Postives = 178/238 (74.79%), Query Frame = 1 
Query:    1 YGSPCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAK 714
            YGS CVKPP+IYGDV RPKAMTV WS  AQS+T + MKGMLTGPVTIL WSFVRND PR   C QI +A+ DEV DLEKAGI VIQIDE A+REGLPL++++   YL WA  +FR+++ GV D TQIHTHMCYS FNDI+ +I  +DADVITIE SRSD +LL+ F +  KY   IGPGVYDIHSPR+P+ EEI   + K L V+    LWVNPDCGLKTR ++E    L  M++  K
Sbjct:  514 YGSRCVKPPVIYGDVVRPKAMTVRWSKYAQSLTKKVMKGMLTGPVTILQWSFVRNDIPRSTVCKQIGVALSDEVLDLEKAGIKVIQIDEPAIREGLPLKRADWDTYLQWAGEAFRLSSMGVADDTQIHTHMCYSEFNDILPAIAALDADVITIETSRSDMELLTAFGD-FKYPNDIGPGVYDIHSPRVPTAEEIEHLLRKALNVVPKERLWVNPDCGLKTRGWSETIAQLEVMMSVTK 750
[Close]
BLAST of CF832574 vs. ExPASy Swiss-Prot
Match: METE_NITEU (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Nitrosomonas europaea GN=metE PE=3 SV=1)

HSP 1 Score: 300.442 bits (768), Expect = 6.100e-81
Identity = 147/238 (61.76%), Postives = 177/238 (74.37%), Query Frame = 1 
Query:    1 YGSPCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAK 714
            YGS CVKPPI+YGDV RP+AMTV W   AQS T +P+KGMLTGPVT+L WSFVR+DQPR  T  Q+ALAI+DEV DLE AGI +IQIDE A REGLPLRK +   YLDWAV +FR+ + GV+D TQIHTHMCYS F+DI+ +I ++DADVITIE SRS  +LL  F +   Y   IGPGVYDIHSPR+P   E+ + + K    ++  +LWVNPDCGLKTR + E + AL  MV  AK
Sbjct:  514 YGSRCVKPPILYGDVYRPEAMTVEWIKYAQSQTGKPVKGMLTGPVTMLMWSFVRDDQPRSTTALQLALAIRDEVADLENAGIGMIQIDEPAFREGLPLRKQDWKSYLDWAVKAFRVASSGVKDETQIHTHMCYSEFHDILPAIAELDADVITIETSRSRMELLDAFVK-FSYPNEIGPGVYDIHSPRVPDASEMFELLKKASRYIDPTLLWVNPDCGLKTRNWPETQTALQKMVDCAK 750
[Close]
BLAST of CF832574 vs. ExPASy Swiss-Prot
Match: METE_ACIF5 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Acidithiobacillus ferrooxidans (strain ATCC 53993) GN=metE PE=3 SV=1)

HSP 1 Score: 300.442 bits (768), Expect = 6.100e-81
Identity = 151/238 (63.45%), Postives = 174/238 (73.11%), Query Frame = 1 
Query:    1 YGSPCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAK 714
            YGS  VKPP+I+GDVSRP  MTV WS  AQS+T RPMKGMLTGPVTIL WSFVR+DQPR  T  QIALAI+DEV DL  AGI +IQIDE A REGLPL++ +   YL+WA  +FRI+     D  QIHTHMCYS FNDI+ +I  MDADVITIE SRS  +LL  F     Y   IGPGVYDIHSPR+PS EE+   + K + V+    LW+NPDCGLKTRK+ EV PAL NMV AA+
Sbjct:  541 YGSRYVKPPLIFGDVSRPTPMTVAWSKYAQSLTQRPMKGMLTGPVTILQWSFVRDDQPRERTALQIALAIRDEVRDLIDAGIGIIQIDEPAYREGLPLKRKDWGHYLEWASRAFRISAQIAPDDVQIHTHMCYSEFNDILPAIAAMDADVITIETSRSQMELLDAFAT-FNYPNEIGPGVYDIHSPRVPSVEEMVGLMEKAVKVVPAERLWINPDCGLKTRKWAEVTPALENMVEAAR 777
[Close]
BLAST of CF832574 vs. ExPASy Swiss-Prot
Match: METE_ACIF2 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Acidithiobacillus ferrooxidans (strain ATCC 23270 / DSM 14882 / NCIB 8455) GN=metE PE=3 SV=1)

HSP 1 Score: 300.442 bits (768), Expect = 6.100e-81
Identity = 151/238 (63.45%), Postives = 174/238 (73.11%), Query Frame = 1 
Query:    1 YGSPCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAK 714
            YGS  VKPP+I+GDVSRP  MTV WS  AQS+T RPMKGMLTGPVTIL WSFVR+DQPR  T  QIALAI+DEV DL  AGI +IQIDE A REGLPL++ +   YL+WA  +FRI+     D  QIHTHMCYS FNDI+ +I  MDADVITIE SRS  +LL  F     Y   IGPGVYDIHSPR+PS EE+   + K + V+    LW+NPDCGLKTRK+ EV PAL NMV AA+
Sbjct:  541 YGSRYVKPPLIFGDVSRPTPMTVAWSKYAQSLTQRPMKGMLTGPVTILQWSFVRDDQPRERTALQIALAIRDEVRDLIDAGIGIIQIDEPAYREGLPLKRKDWGHYLEWASRAFRISAQIAPDDVQIHTHMCYSEFNDILPAIAAMDADVITIETSRSQMELLDAFAT-FNYPNEIGPGVYDIHSPRVPSVEEMVGLMEKAVKVVPAERLWINPDCGLKTRKWAEVTPALENMVEAAR 777
[Close]
BLAST of CF832574 vs. ExPASy Swiss-Prot
Match: METE_METCA (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Methylococcus capsulatus GN=metE PE=3 SV=1)

HSP 1 Score: 300.056 bits (767), Expect = 7.967e-81
Identity = 148/238 (62.18%), Postives = 177/238 (74.37%), Query Frame = 1 
Query:    1 YGSPCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAK 714
            YGS CVKPPIIYGDV+RP+ MTV W   AQS+T +P+KGMLTGPVT+L WSF R+DQPR  T  QIALAI+DEV DLE AG+  IQIDE A REGLPL+ ++ A YLDWAV +FR+ +CGV D TQIHTHMCYS F DI+ +I ++DADVITIE  RS   LL  F     Y   IGPGVYDIHSPR+P+  EI   + K  A ++   LWVNPDCGLKTR++ E + AL+NMV AA+
Sbjct:  513 YGSRCVKPPIIYGDVARPEPMTVEWIGYAQSLTKKPVKGMLTGPVTMLQWSFARDDQPRAVTALQIALAIRDEVADLEAAGVAAIQIDEPAFREGLPLKAADRADYLDWAVKAFRLASCGVADDTQIHTHMCYSEFGDILPAIAELDADVITIETCRSAMDLLEAF-GAFGYPNDIGPGVYDIHSPRVPAGAEIRALLEKAAAAVDPERLWVNPDCGLKTRQWPEAEQALANMVEAAR 749
[Close]
BLAST of CF832574 vs. ExPASy Swiss-Prot
Match: METE_PARL1 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Parvibaculum lavamentivorans (strain DS-1 / DSM 13023 / NCIMB 13966) GN=metE PE=3 SV=1)

HSP 1 Score: 299.286 bits (765), Expect = 1.359e-80
Identity = 151/238 (63.45%), Postives = 177/238 (74.37%), Query Frame = 1 
Query:    1 YGSPCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAK 714
            YGS CVKPPII+GDVSRP+ MTV WS  AQS+T RP+KGMLTGPVTIL WSFVR+DQPR ETC QIALAI+DEV DLE AG+ +IQIDE ALREGLPL +SE   YL WA   FR++ C  +D TQIHTHMCYS FNDII +I D+DADVI+IE SRS  +LL  F +   Y   IGPGVYDIHSPR+P+  E+   +      L +  +WVNPDCGLKTR + E + AL  MV AA+
Sbjct:  519 YGSRCVKPPIIFGDVSRPEPMTVEWSRYAQSLTDRPVKGMLTGPVTILQWSFVRDDQPRSETCRQIALAIRDEVYDLEAAGLGMIQIDEPALREGLPLHRSEWDTYLRWACECFRLSVCVARDETQIHTHMCYSEFNDIIEAIADLDADVISIETSRSRMELLDAFVD-FSYPNDIGPGVYDIHSPRVPARGEMLSLLRLAAERLPSERIWVNPDCGLKTRGWPETEAALVEMVEAAR 755
[Close]
BLAST of CF832574 vs. ExPASy Swiss-Prot
Match: METE_COXB1 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Coxiella burnetii (strain CbuK_Q154) GN=metE PE=3 SV=1)

HSP 1 Score: 299.286 bits (765), Expect = 1.359e-80
Identity = 148/238 (62.18%), Postives = 179/238 (75.21%), Query Frame = 1 
Query:    1 YGSPCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAK 714
            YGS CVKPPII+GDVSR + MT+ W   AQS+T + +KGMLTGPVTIL WSFVR+DQPR +T  QIALA++DEV+DLE++G+ VIQIDE A RE LPLRK+    YL+WAV  FR+ +CGV+D TQIHTHMCYS FNDII +I  +DADVITIE+SRS+ ++L  F E   Y   IGPG+YDIHSPRIP   EI +   + L  +    LW+NPDCGLKTR + E K ALS MV AAK
Sbjct:  516 YGSRCVKPPIIFGDVSRERPMTLRWIEYAQSLTTKSVKGMLTGPVTILAWSFVRDDQPRSQTAKQIALALRDEVQDLERSGVRVIQIDEPAFRECLPLRKAAWQDYLEWAVKCFRLASCGVKDETQIHTHMCYSEFNDIIEAIAALDADVITIESSRSETEILKSF-EKFAYPNDIGPGIYDIHSPRIPRVAEIEELAVRALQYIPIERLWINPDCGLKTRNWEETKEALSRMVDAAK 752
[Close]
BLAST of CF832574 vs. ExPASy Swiss-Prot
Match: METE_HAEIN (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Haemophilus influenzae GN=metE PE=3 SV=1)

HSP 1 Score: 298.901 bits (764), Expect = 1.775e-80
Identity = 149/238 (62.61%), Postives = 176/238 (73.95%), Query Frame = 1 
Query:    1 YGSPCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAK 714
            YGS CVKPP+IYGDV+RP+ MTV WS  AQS+T R MKGMLTGPVTIL WSFVRND PR   C QI +A+ DEV DLE AGI VIQIDE A+REGLPL++++   YL WA  +FR+++ GVQD TQIHTHMCYS FNDI+ +I  +DADVITIE SRSD +LL+ F +  KY   IGPGVYDIHSPR+P+  E+   + K L V+    LWVNPDCGLKTR +TE    L  MV   K
Sbjct:  513 YGSRCVKPPVIYGDVTRPEPMTVRWSQYAQSLTNRVMKGMLTGPVTILQWSFVRNDIPRSTVCKQIGVALSDEVLDLEAAGIKVIQIDEPAIREGLPLKRADWDAYLQWAGEAFRLSSMGVQDDTQIHTHMCYSEFNDILPAIAALDADVITIETSRSDMELLTAFAD-FKYPNDIGPGVYDIHSPRVPTATEVEHLLRKALNVIPKERLWVNPDCGLKTRGWTETIDQLKVMVDVTK 749
[Close]
BLAST of CF832574 vs. ExPASy Swiss-Prot
Match: METE_HAEIE (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Haemophilus influenzae (strain PittEE) GN=metE PE=3 SV=1)

HSP 1 Score: 298.901 bits (764), Expect = 1.775e-80
Identity = 149/238 (62.61%), Postives = 176/238 (73.95%), Query Frame = 1 
Query:    1 YGSPCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAK 714
            YGS CVKPP+IYGDV+RP+ MTV WS  AQS+T R MKGMLTGPVTIL WSFVRND PR   C QI +A+ DEV DLE AGI VIQIDE A+REGLPL++++   YL WA  +FR+++ GVQD TQIHTHMCYS FNDI+ +I  +DADVITIE SRSD +LL+ F +  KY   IGPGVYDIHSPR+P+  E+   + K L V+    LWVNPDCGLKTR +TE    L  MV   K
Sbjct:  513 YGSRCVKPPVIYGDVTRPEPMTVRWSQYAQSLTNRVMKGMLTGPVTILQWSFVRNDIPRSTVCKQIGVALSDEVLDLEAAGIKVIQIDEPAIREGLPLKRADWDAYLQWAGEAFRLSSMGVQDDTQIHTHMCYSEFNDILPAIAALDADVITIETSRSDMELLTAFAD-FKYPNDIGPGVYDIHSPRVPTAAEVEHLLRKALNVIPKERLWVNPDCGLKTRGWTETIDQLKVMVDVTK 749
[Close]
BLAST of CF832574 vs. ExPASy Swiss-Prot
Match: METE_COXBU (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Coxiella burnetii GN=metE PE=3 SV=1)

HSP 1 Score: 298.901 bits (764), Expect = 1.775e-80
Identity = 148/238 (62.18%), Postives = 179/238 (75.21%), Query Frame = 1 
Query:    1 YGSPCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAK 714
            YGS CVKPPII+GDVSR + MT+ W   AQS+T + +KGMLTGPVTIL WSFVR+DQPR +T  QIALA++DEV+DLE++G+ VIQIDE A RE LPLRK+    YL+WAV  FR+ +CGV+D TQIHTHMCYS FNDII +I  +DADVITIE+SRS+ ++L  F E   Y   IGPG+YDIHSPRIP   EI +   + L  +    LW+NPDCGLKTR + E K ALS MV AAK
Sbjct:  516 YGSRCVKPPIIFGDVSRERPMTLRWIEYAQSLTTKSVKGMLTGPVTILAWSFVRDDQPRSQTAKQIALALRDEVQDLERSGVRVIQIDEPAFRECLPLRKAAWQDYLEWAVKCFRLASCGVKDETQIHTHMCYSEFNDIIEAIAALDADVITIESSRSEMEILKSF-EKFAYPNDIGPGIYDIHSPRIPRVAEIEELAVRALQYIPIERLWINPDCGLKTRNWEETKEALSRMVDAAK 752
The following BLAST results are available for this feature:
BLAST of CF832574 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 286
Match NameE-valueIdentityDescription
METE_HAES14.671e-8163.035-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_NITEU6.100e-8161.765-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_ACIF56.100e-8163.455-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_ACIF26.100e-8163.455-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_METCA7.967e-8162.185-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_PARL11.359e-8063.455-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_COXB11.359e-8062.185-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_HAEIN1.775e-8062.615-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_HAEIE1.775e-8062.615-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_COXBU1.775e-8062.185-methyltetrahydropteroyltriglutamate--homocystein... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS02_02B06_r Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa02B06, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF832574 ID=CF832574; Name=CF832574; organism=Citrus sinensis; type=EST; length=716bp
TATGGATCTCCCTGTGTGAAGCCACCCATCATCTATGGTGATGTGAGCCG
TCCCAAGGCTATGACCGTCTTCTGGTCCTCCTTGGCTCAGAGCATGACAG
CTCGCCCAATGAAAGGAATGCTTACTGGCCCTGTCACCATTCTCAACTGG
TCCTTTGTTAGAAATGACCAGCCCAGACATGAAACCTGCTACCAGATTGC
TTTGGCCATCAAGGACGAAGTCGAGGATCTTGAGAAGGCAGGCATTAACG
TTATTCAAATTGATGAGGCTGCCTTGAGGGAAGGATTGCCTCTTAGGAAA
TCTGAGCATGCTTTCTACTTGGACTGGGCAGTCCACTCCTTCAGAATCAC
CAACTGTGGCGTCCAGGACACTACCCAGATCCACACCCACATGTGCTACT
CCAACTTCAACGACATTATCCACTCAATTATCGACATGGATGCTGATGTG
ATCACCATTGAGAACTCTCGTTCTGATGAGAAGCTCCTGTCTGTCTTCCG
TGAGGGAGTCAAGTATGGTGCTGGAATTGGCCCTGGTGTGTATGACATCC
ACTCTCCAAGAATACCATCAACTGAAGAAATTGCTGACAGAATCAACAAG
ATGCTTGCTGTGCTAGAAACCAACATTTTGTGGGTTAACCCTGACTGTGG
TCTTAAGACTCGCAAGTACACTGAGGTGAAGCCAGCCCTCTCAAACATGG
TCGCTGCTGCCAAGCT
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