CF832574

Overview
NameCF832574
Unique NameCF832574
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length716
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig9752 contig Csv1_Contig9752:92..807. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CF832574 vs. ExPASy Swiss-Prot
Match: METE_COXBR (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Coxiella burnetii (strain RSA 331 / Henzerling II) GN=metE PE=3 SV=1)

HSP 1 Score: 298.901 bits (764), Expect = 1.775e-80
Identity = 148/238 (62.18%), Postives = 179/238 (75.21%), Query Frame = 1 
Query:    1 YGSPCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAK 714
            YGS CVKPPII+GDVSR + MT+ W   AQS+T + +KGMLTGPVTIL WSFVR+DQPR +T  QIALA++DEV+DLE++G+ VIQIDE A RE LPLRK+    YL+WAV  FR+ +CGV+D TQIHTHMCYS FNDII +I  +DADVITIE+SRS+ ++L  F E   Y   IGPG+YDIHSPRIP   EI +   + L  +    LW+NPDCGLKTR + E K ALS MV AAK
Sbjct:  516 YGSRCVKPPIIFGDVSRERPMTLRWIEYAQSLTTKSVKGMLTGPVTILAWSFVRDDQPRSQTAKQIALALRDEVQDLERSGVRVIQIDEPAFRECLPLRKAAWQDYLEWAVKCFRLASCGVKDETQIHTHMCYSEFNDIIEAIAALDADVITIESSRSEMEILKSF-EKFAYPNDIGPGIYDIHSPRIPRVAEIEELAVRALQYIPIERLWINPDCGLKTRNWEETKEALSRMVDAAK 752
[Close]
BLAST of CF832574 vs. ExPASy Swiss-Prot
Match: METE_COXBN (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Coxiella burnetii (strain Dugway 5J108-111) GN=metE PE=3 SV=1)

HSP 1 Score: 298.901 bits (764), Expect = 1.775e-80
Identity = 148/238 (62.18%), Postives = 179/238 (75.21%), Query Frame = 1 
Query:    1 YGSPCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAK 714
            YGS CVKPPII+GDVSR + MT+ W   AQS+T + +KGMLTGPVTIL WSFVR+DQPR +T  QIALA++DEV+DLE++G+ VIQIDE A RE LPLRK+    YL+WAV  FR+ +CGV+D TQIHTHMCYS FNDII +I  +DADVITIE+SRS+ ++L  F E   Y   IGPG+YDIHSPRIP   EI +   + L  +    LW+NPDCGLKTR + E K ALS MV AAK
Sbjct:  516 YGSRCVKPPIIFGDVSRERPMTLRWIEYAQSLTTKSVKGMLTGPVTILAWSFVRDDQPRSQTAKQIALALRDEVQDLERSGVRVIQIDEPAFRECLPLRKAAWQDYLEWAVKCFRLASCGVKDETQIHTHMCYSEFNDIIEAIAALDADVITIESSRSEMEILKSF-EKFAYPNDIGPGIYDIHSPRIPRVAEIEELAVRALQYIPIERLWINPDCGLKTRNWEETKEALSRMVDAAK 752
[Close]
BLAST of CF832574 vs. ExPASy Swiss-Prot
Match: METE_COXB2 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Coxiella burnetii (strain CbuG_Q212) GN=metE PE=3 SV=1)

HSP 1 Score: 298.901 bits (764), Expect = 1.775e-80
Identity = 148/238 (62.18%), Postives = 179/238 (75.21%), Query Frame = 1 
Query:    1 YGSPCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAK 714
            YGS CVKPPII+GDVSR + MT+ W   AQS+T + +KGMLTGPVTIL WSFVR+DQPR +T  QIALA++DEV+DLE++G+ VIQIDE A RE LPLRK+    YL+WAV  FR+ +CGV+D TQIHTHMCYS FNDII +I  +DADVITIE+SRS+ ++L  F E   Y   IGPG+YDIHSPRIP   EI +   + L  +    LW+NPDCGLKTR + E K ALS MV AAK
Sbjct:  516 YGSRCVKPPIIFGDVSRERPMTLRWIEYAQSLTTKSVKGMLTGPVTILAWSFVRDDQPRSQTAKQIALALRDEVQDLERSGVRVIQIDEPAFRECLPLRKAAWQDYLEWAVKCFRLASCGVKDETQIHTHMCYSEFNDIIEAIAALDADVITIESSRSEMEILKSF-EKFAYPNDIGPGIYDIHSPRIPRVAEIEELAVRALQYIPIERLWINPDCGLKTRNWEETKEALSRMVDAAK 752
[Close]
BLAST of CF832574 vs. ExPASy Swiss-Prot
Match: METE_RALEU (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Ralstonia eutropha GN=metE PE=3 SV=1)

HSP 1 Score: 298.13 bits (762), Expect = 3.027e-80
Identity = 150/238 (63.03%), Postives = 183/238 (76.89%), Query Frame = 1 
Query:    1 YGSPCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAK 714
            YGS CVKPP+IYGDV RP+ MTV  +  AQS+T RPMKGMLTGP+T+L WSFVR+DQPR  T  Q+ALAI+DEV DLE+AGI VIQIDE ALREGLPLR+++   YLDWAV +FR++  GVQD TQIHTHMCY+ FNDI+ +I  MDADVITIE SRS  +LL  F +   Y   IGPGVYDIHSPR+PS + +   +++   V+    LWVNPDCGLKTR + E + AL+NMV+AA+
Sbjct:  513 YGSRCVKPPVIYGDVYRPEPMTVDTARYAQSLTERPMKGMLTGPITMLQWSFVRDDQPRATTARQLALAIRDEVCDLEQAGIRVIQIDEPALREGLPLRRADWDAYLDWAVTAFRLSASGVQDQTQIHTHMCYAEFNDILPAIAAMDADVITIETSRSAMELLEGFGD-FDYPNEIGPGVYDIHSPRVPSVQAMERLLDRACEVVPPQRLWVNPDCGLKTRGWEETEAALANMVSAAR 749
[Close]
BLAST of CF832574 vs. ExPASy Swiss-Prot
Match: METE_RALEH (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Ralstonia eutropha (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) GN=metE PE=3 SV=1)

HSP 1 Score: 298.13 bits (762), Expect = 3.027e-80
Identity = 150/238 (63.03%), Postives = 183/238 (76.89%), Query Frame = 1 
Query:    1 YGSPCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAK 714
            YGS CVKPP+IYGDV RP+ MTV  +  AQS+T RPMKGMLTGP+T+L WSFVR+DQPR  T  Q+ALAI+DEV DLE+AGI VIQIDE ALREGLPLR+++   YLDWAV +FR++  GVQD TQIHTHMCY+ FNDI+ +I  MDADVITIE SRS  +LL  F +   Y   IGPGVYDIHSPR+PS + +   +++   V+    LWVNPDCGLKTR + E + AL+NMV+AA+
Sbjct:  513 YGSRCVKPPVIYGDVYRPEPMTVDTARYAQSLTERPMKGMLTGPITMLQWSFVRDDQPRATTARQLALAIRDEVCDLEQAGIRVIQIDEPALREGLPLRRADWDAYLDWAVTAFRLSASGVQDQTQIHTHMCYAEFNDILPAIAAMDADVITIETSRSAMELLEGFGD-FDYPNEIGPGVYDIHSPRVPSVQAMERLLDRACEVVPPQRLWVNPDCGLKTRGWEETEAALANMVSAAR 749
[Close]
BLAST of CF832574 vs. ExPASy Swiss-Prot
Match: METE_NITOC (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Nitrosococcus oceani (strain ATCC 19707 / NCIMB 11848) GN=metE PE=3 SV=1)

HSP 1 Score: 297.745 bits (761), Expect = 3.954e-80
Identity = 148/237 (62.45%), Postives = 176/237 (74.26%), Query Frame = 1 
Query:    1 YGSPCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAA 711
            YGS CVKPPIIYGDV R + MTV W   AQS+T+R +KGMLTGPVT+L WSFVR+DQPR +TC Q+AL ++DEV DLE+A I +IQ+DE A REGLPLR+ E   YL WAV  FR+ + GV+D TQIHTHMCYS FNDIIH+I  +DADVITIE SRSD +LL+ F    +Y   IGPGVYDIHSP +P+  +I   I K    +    LWVNPDCGLKTR + EV+ AL  MVAAA
Sbjct:  517 YGSRCVKPPIIYGDVVRRQPMTVAWLRYAQSLTSRRVKGMLTGPVTLLQWSFVRDDQPRADTCLQLALVLRDEVRDLEQADIKLIQVDEPAFREGLPLRRVEWESYLAWAVRCFRVASSGVEDGTQIHTHMCYSEFNDIIHAIAALDADVITIETSRSDGELLAAF-AAFEYPNEIGPGVYDIHSPVVPTEAQIVGLIKKAAEYIPAERLWVNPDCGLKTRAWPEVETALRTMVAAA 752
[Close]
BLAST of CF832574 vs. ExPASy Swiss-Prot
Match: METE_HAEIG (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Haemophilus influenzae (strain PittGG) GN=metE PE=3 SV=1)

HSP 1 Score: 297.745 bits (761), Expect = 3.954e-80
Identity = 149/238 (62.61%), Postives = 175/238 (73.53%), Query Frame = 1 
Query:    1 YGSPCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAK 714
            YGS CVKPP+IYGDV RP+ MTV WS  AQS+T R MKGMLTGPVTIL WSFVRND PR   C QI +A+ DEV DLE AGI VIQIDE A+REGLPL++++   YL WA  +FR+++ GVQD TQIHTHMCYS FNDI+ +I  +DADVITIE SRSD +LL+ F +  KY   IGPGVYDIHSPR+P+  E+   + K L V+    LWVNPDCGLKTR +TE    L  MV   K
Sbjct:  513 YGSRCVKPPVIYGDVIRPEPMTVRWSQYAQSLTNRVMKGMLTGPVTILQWSFVRNDIPRSTVCKQIGVALSDEVLDLEAAGIKVIQIDEPAIREGLPLKRADWDAYLQWAGEAFRLSSMGVQDDTQIHTHMCYSEFNDILPAIAALDADVITIETSRSDMELLTAFAD-FKYPNDIGPGVYDIHSPRVPTATEVEHLLRKALNVIPKERLWVNPDCGLKTRGWTETIDQLKVMVDVTK 749
[Close]
BLAST of CF832574 vs. ExPASy Swiss-Prot
Match: METE_RHILO (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Rhizobium loti GN=metE PE=3 SV=1)

HSP 1 Score: 297.36 bits (760), Expect = 5.164e-80
Identity = 151/238 (63.45%), Postives = 176/238 (73.95%), Query Frame = 1 
Query:    1 YGSPCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAK 714
            YGS  V+PPII GDVSR   MT+ W   AQS+T +P+KGMLTGPVTILNWSFVR+D PR   C Q+ALAI+DEV DLEKAG  +IQIDEAALREGLPLR+++   YLDWAV  FR+ +  V D TQIHTHMCYS F DI+ +I  MDADVI+IE SRS  +LL   R   KY   IGPGVYDIHSPR+P   EI++ I      L    LWVNPDCGLKTR++ EV+PAL NMVAAA+
Sbjct:  531 YGSRFVRPPIIIGDVSRQNPMTLHWWRYAQSLTPKPVKGMLTGPVTILNWSFVRDDLPRSAVCRQVALAIRDEVSDLEKAGAKMIQIDEAALREGLPLREADWQAYLDWAVECFRLASTAVGDATQIHTHMCYSEFGDIVDAIAAMDADVISIETSRSQLELLDTLRT-FKYPNEIGPGVYDIHSPRVPEVGEISNLIMLARKRLSDGQLWVNPDCGLKTRRWEEVRPALVNMVAAAR 767
[Close]
BLAST of CF832574 vs. ExPASy Swiss-Prot
Match: METE_HAES2 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Haemophilus somnus (strain 2336) GN=metE PE=3 SV=1)

HSP 1 Score: 297.36 bits (760), Expect = 5.164e-80
Identity = 148/238 (62.18%), Postives = 177/238 (74.37%), Query Frame = 1 
Query:    1 YGSPCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAK 714
            YGS CVKPP+IYGDV RP+ MTV WS  AQS+T + MKGMLTGPVTIL WSFVRND PR   C QI +A+ DEV DLEKAGI VIQIDE A+REGLPL++++   YL WA  +FR+++ GV D TQIHTHMCYS FNDI+ +I  +DADVITIE SRSD +LL+ F +  KY   IGPGVYDIHSPR+P+ EEI   + K L V+    LWVNPDCGLKTR ++E    L  M++  K
Sbjct:  514 YGSRCVKPPVIYGDVVRPEPMTVRWSKYAQSLTKKVMKGMLTGPVTILQWSFVRNDIPRSTVCKQIGVALSDEVLDLEKAGIKVIQIDEPAIREGLPLKRADWDTYLQWAGEAFRLSSMGVADDTQIHTHMCYSEFNDILPAIAALDADVITIETSRSDMELLTAFGD-FKYPNDIGPGVYDIHSPRVPTAEEIEHLLRKALNVVPKERLWVNPDCGLKTRGWSETIAQLEVMMSVTK 750
[Close]
BLAST of CF832574 vs. ExPASy Swiss-Prot
Match: METE_SHEON (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Shewanella oneidensis GN=metE PE=3 SV=1)

HSP 1 Score: 296.975 bits (759), Expect = 6.744e-80
Identity = 150/238 (63.03%), Postives = 176/238 (73.95%), Query Frame = 1 
Query:    1 YGSPCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAK 714
            YGS CVKPP+IYGDVSRPKAMTV W+  AQS+T +P+KGMLTGPVTIL+WSF R D  R     Q+ALAI+DEV DL+ AGI +IQIDE A REGLPL++SE   YLDWAV++F+++  GV D TQIHTHMCYS FND I +I  MDADVITIE SRS  +LL+ F E  +Y   IGPGVYDIHSP  PS E +   I K    +    LWVNPDCGLKTR + EV+PAL NMV A +
Sbjct:  517 YGSRCVKPPLIYGDVSRPKAMTVDWAVFAQSLTDKPVKGMLTGPVTILHWSFAREDISRDTIATQLALAIRDEVVDLQHAGIGIIQIDEPAFREGLPLKQSEWQAYLDWAVNAFKLSAAGVVDETQIHTHMCYSEFNDTIAAIAAMDADVITIETSRSRMELLNAF-EDFEYPNEIGPGVYDIHSPNTPSVEAMVHLIEKAAQKVPVRQLWVNPDCGLKTRTWDEVEPALKNMVDATR 753
The following BLAST results are available for this feature:
BLAST of CF832574 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 286
Match NameE-valueIdentityDescription
METE_COXBR1.775e-8062.185-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_COXBN1.775e-8062.185-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_COXB21.775e-8062.185-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_RALEU3.027e-8063.035-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_RALEH3.027e-8063.035-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_NITOC3.954e-8062.455-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_HAEIG3.954e-8062.615-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_RHILO5.164e-8063.455-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_HAES25.164e-8062.185-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_SHEON6.744e-8063.035-methyltetrahydropteroyltriglutamate--homocystein... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS02_02B06_r Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa02B06, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF832574 ID=CF832574; Name=CF832574; organism=Citrus sinensis; type=EST; length=716bp
TATGGATCTCCCTGTGTGAAGCCACCCATCATCTATGGTGATGTGAGCCG
TCCCAAGGCTATGACCGTCTTCTGGTCCTCCTTGGCTCAGAGCATGACAG
CTCGCCCAATGAAAGGAATGCTTACTGGCCCTGTCACCATTCTCAACTGG
TCCTTTGTTAGAAATGACCAGCCCAGACATGAAACCTGCTACCAGATTGC
TTTGGCCATCAAGGACGAAGTCGAGGATCTTGAGAAGGCAGGCATTAACG
TTATTCAAATTGATGAGGCTGCCTTGAGGGAAGGATTGCCTCTTAGGAAA
TCTGAGCATGCTTTCTACTTGGACTGGGCAGTCCACTCCTTCAGAATCAC
CAACTGTGGCGTCCAGGACACTACCCAGATCCACACCCACATGTGCTACT
CCAACTTCAACGACATTATCCACTCAATTATCGACATGGATGCTGATGTG
ATCACCATTGAGAACTCTCGTTCTGATGAGAAGCTCCTGTCTGTCTTCCG
TGAGGGAGTCAAGTATGGTGCTGGAATTGGCCCTGGTGTGTATGACATCC
ACTCTCCAAGAATACCATCAACTGAAGAAATTGCTGACAGAATCAACAAG
ATGCTTGCTGTGCTAGAAACCAACATTTTGTGGGTTAACCCTGACTGTGG
TCTTAAGACTCGCAAGTACACTGAGGTGAAGCCAGCCCTCTCAAACATGG
TCGCTGCTGCCAAGCT
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