CF832574

Overview
NameCF832574
Unique NameCF832574
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length716
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig9752 contig Csv1_Contig9752:92..807. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CF832574 vs. ExPASy Swiss-Prot
Match: METE_BLOFL (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Blochmannia floridanus GN=metE PE=3 SV=1)

HSP 1 Score: 296.975 bits (759), Expect = 6.744e-80
Identity = 140/238 (58.82%), Postives = 181/238 (76.05%), Query Frame = 1 
Query:    1 YGSPCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAK 714
            YGS C+KPPII GD+SRP  +T+ W + AQS+T +P+KG+LTGP+TI+ WSF R D PRH    Q+ALAI+DEV DLEK+GI VIQIDE ALREGLPL+KSE ++YL WA+++F+IT   V+D TQIHTHMCYS FN+II  I+ +D DVI+IE+SRSD +LL   +   K    IGPG+YDIHS   PS  EI D++ K+L  +  + LW+NPDCGLKTR + E++ +L+NMV AAK
Sbjct:  522 YGSRCIKPPIIIGDISRPIPITIPWINYAQSLTNKPVKGILTGPITIMTWSFAREDIPRHMIALQLALAIRDEVMDLEKSGIGVIQIDEPALREGLPLKKSEQSYYLKWAINTFKITVSSVKDNTQIHTHMCYSEFNEIIDDILKLDVDVISIESSRSDLQLLQFIKTAGKKLNEIGPGIYDIHSIHQPSINEIMDKLKKLLQYIPKDRLWINPDCGLKTRSWNEIRESLNNMVIAAK 759
[Close]
BLAST of CF832574 vs. ExPASy Swiss-Prot
Match: METE_PASMU (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Pasteurella multocida GN=metE PE=3 SV=1)

HSP 1 Score: 296.204 bits (757), Expect = 1.150e-79
Identity = 149/238 (62.61%), Postives = 174/238 (73.11%), Query Frame = 1 
Query:    1 YGSPCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAK 714
            YGS CVKPP+IYGDV RP+ MTV WS  AQS+T + MKGMLTGPVTIL WSFVRND PR   C QI +A+ DEV DLEKAGI VIQIDE A+REGLPL++++   YL WA  +FR++  GVQD TQIHTHMCYS FNDI+ +I  +DADVITIE SRSD +LL+ F +  KY   IGPGVYDIHSPR+P  EEI   + K L V+    LWVNPDCGLKTR + E    L  M+   K
Sbjct:  514 YGSRCVKPPVIYGDVVRPEPMTVRWSQYAQSLTNKVMKGMLTGPVTILQWSFVRNDIPRSTVCKQIGVALSDEVLDLEKAGIKVIQIDEPAIREGLPLKRADWDAYLQWAGEAFRLSYMGVQDDTQIHTHMCYSEFNDILPAIAGLDADVITIETSRSDMELLTAFGD-FKYPNDIGPGVYDIHSPRVPKAEEIERLLRKALNVVPKERLWVNPDCGLKTRGWPETIAQLEVMMEVTK 750
[Close]
BLAST of CF832574 vs. ExPASy Swiss-Prot
Match: METE_ACTPJ (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Actinobacillus pleuropneumoniae serotype 3 (strain JL03) GN=metE PE=3 SV=1)

HSP 1 Score: 296.204 bits (757), Expect = 1.150e-79
Identity = 148/238 (62.18%), Postives = 175/238 (73.53%), Query Frame = 1 
Query:    1 YGSPCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAK 714
            YGS CVKPP+IYGDV+RP+ MTV WS  AQS+T + MKGMLTGPVTIL WSFVRND PR   C QI +A+ DEV DLEKAGI VIQIDE A+REGLPL++++   YL WA  +FR++  GV+D TQIHTHMCYS FNDI+ +I  +DADVITIE SRSD +LL+ F +  KY   IGPGVYDIHSPR+P+  EI   + K L V+    LWVNPDCGLKTR + E    L  MV   K
Sbjct:  513 YGSRCVKPPVIYGDVTRPEPMTVRWSQYAQSLTDKVMKGMLTGPVTILQWSFVRNDIPRSTVCKQIGVALSDEVLDLEKAGIKVIQIDEPAIREGLPLKRADWDAYLQWAGEAFRLSYMGVKDDTQIHTHMCYSEFNDILPAIAGLDADVITIETSRSDMELLTAFAD-FKYPNDIGPGVYDIHSPRVPTAGEIEHLLRKALKVIPKERLWVNPDCGLKTRGWKETIEQLQVMVEVTK 749
[Close]
BLAST of CF832574 vs. ExPASy Swiss-Prot
Match: METE_ACTP7 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Actinobacillus pleuropneumoniae serotype 7 (strain AP76) GN=metE PE=3 SV=1)

HSP 1 Score: 296.204 bits (757), Expect = 1.150e-79
Identity = 148/238 (62.18%), Postives = 175/238 (73.53%), Query Frame = 1 
Query:    1 YGSPCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAK 714
            YGS CVKPP+IYGDV+RP+ MTV WS  AQS+T + MKGMLTGPVTIL WSFVRND PR   C QI +A+ DEV DLEKAGI VIQIDE A+REGLPL++++   YL WA  +FR++  GV+D TQIHTHMCYS FNDI+ +I  +DADVITIE SRSD +LL+ F +  KY   IGPGVYDIHSPR+P+  EI   + K L V+    LWVNPDCGLKTR + E    L  MV   K
Sbjct:  513 YGSRCVKPPVIYGDVTRPEPMTVRWSQYAQSLTDKVMKGMLTGPVTILQWSFVRNDIPRSTVCKQIGVALSDEVLDLEKAGIKVIQIDEPAIREGLPLKRADWDAYLQWAGEAFRLSYMGVKDDTQIHTHMCYSEFNDILPAIAGLDADVITIETSRSDMELLTAFAD-FKYPNDIGPGVYDIHSPRVPTAGEIEHLLRKALKVIPKERLWVNPDCGLKTRGWKETIEQLQVMVEVTK 749
[Close]
BLAST of CF832574 vs. ExPASy Swiss-Prot
Match: METE_ACTP2 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Actinobacillus pleuropneumoniae serotype 5b (strain L20) GN=metE PE=3 SV=1)

HSP 1 Score: 296.204 bits (757), Expect = 1.150e-79
Identity = 148/238 (62.18%), Postives = 175/238 (73.53%), Query Frame = 1 
Query:    1 YGSPCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAK 714
            YGS CVKPP+IYGDV+RP+ MTV WS  AQS+T + MKGMLTGPVTIL WSFVRND PR   C QI +A+ DEV DLEKAGI VIQIDE A+REGLPL++++   YL WA  +FR++  GV+D TQIHTHMCYS FNDI+ +I  +DADVITIE SRSD +LL+ F +  KY   IGPGVYDIHSPR+P+  EI   + K L V+    LWVNPDCGLKTR + E    L  MV   K
Sbjct:  513 YGSRCVKPPVIYGDVTRPEPMTVRWSQYAQSLTDKVMKGMLTGPVTILQWSFVRNDIPRSTVCKQIGVALSDEVLDLEKAGIKVIQIDEPAIREGLPLKRADWDAYLQWAGEAFRLSYMGVKDDTQIHTHMCYSEFNDILPAIAGLDADVITIETSRSDMELLTAFAD-FKYPNDIGPGVYDIHSPRVPTAGEIEHLLRKALKVIPKERLWVNPDCGLKTRGWKETIEQLQVMVEVTK 749
[Close]
BLAST of CF832574 vs. ExPASy Swiss-Prot
Match: METE_BURXL (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Burkholderia xenovorans (strain LB400) GN=metE PE=3 SV=1)

HSP 1 Score: 295.819 bits (756), Expect = 1.502e-79
Identity = 151/238 (63.45%), Postives = 176/238 (73.95%), Query Frame = 1 
Query:    1 YGSPCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAK 714
            YGS CVKPPIIYGDV RP+ MTV  +  AQS+T R MKGMLTGPVT+L WSFVR+DQPR  T  Q+ALAI+DEV DLEKAGI +IQIDE A REGLPLR+++ A YL+WA  +FRI+  GV D TQIHTHMCYS FNDI+ SI  MDADVITIE SRS  +LL  F     Y   IGPGVYDIHSPR+P  + +   + +   V+    LWVNPDCGLKTR + E + AL+NMV AAK
Sbjct:  512 YGSRCVKPPIIYGDVYRPEPMTVETTRYAQSLTQRLMKGMLTGPVTMLQWSFVRDDQPRSTTALQLALAIRDEVVDLEKAGIRIIQIDEPAFREGLPLRRADWAAYLEWATRAFRISAAGVADQTQIHTHMCYSEFNDILPSIAAMDADVITIETSRSAMELLDGF-GAFAYPNEIGPGVYDIHSPRVPGADAMQRLLERACEVIPAERLWVNPDCGLKTRGWPETEAALANMVRAAK 748
[Close]
BLAST of CF832574 vs. ExPASy Swiss-Prot
Match: METE_PSEU5 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Pseudomonas stutzeri (strain A1501) GN=metE PE=3 SV=1)

HSP 1 Score: 295.434 bits (755), Expect = 1.962e-79
Identity = 150/238 (63.03%), Postives = 170/238 (71.43%), Query Frame = 1 
Query:    1 YGSPCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAK 714
            YGS CVKP IIYGD+SRP+ MTV W   AQ  T R MKGMLTGPVT+L WSF R D PR     Q+ALAI+DEV DLE AGI +IQIDEAA REGLPLR+ +   YLDWAV +FR+   GV+D TQIHTHMCYS FND+I SI  MDADVITIE SRS  +LL  FR    Y   IGPGVYDIHSPR+P T E+   + K    +    LWVNPDCGLKTR + E + AL NMVAAA+
Sbjct:  524 YGSRCVKPAIIYGDLSRPRPMTVDWIRYAQQQTDRIMKGMLTGPVTMLMWSFAREDVPREVQARQLALAIRDEVCDLEAAGIRIIQIDEAAFREGLPLRREQWRHYLDWAVEAFRLCASGVRDETQIHTHMCYSEFNDVIESIAAMDADVITIETSRSQMELLEAFR-AFDYPNDIGPGVYDIHSPRVPDTAEMVQLLEKAAECIPAERLWVNPDCGLKTRGWPETEAALVNMVAAAR 760
[Close]
BLAST of CF832574 vs. ExPASy Swiss-Prot
Match: METE_PSESM (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Pseudomonas syringae pv. tomato GN=metE PE=3 SV=1)

HSP 1 Score: 294.278 bits (752), Expect = 4.372e-79
Identity = 150/238 (63.03%), Postives = 174/238 (73.11%), Query Frame = 1 
Query:    1 YGSPCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAK 714
            YGS CVKP II+GD+SRP+ MTV W   AQS+T + MKGMLTGPVT+L WSF R D  R     Q+ALAI+DEV DLE+AGI ++QIDEAA REGLPLRK++   YLDWAV +FR+   GV+D TQIHTHMCYS FND+I SI  MDADVITIE SRSD +LL  F E   Y   IGPGVYDIHSPR+P T E+   I K    +    LWVNPDCGLKTR + E + AL NMVAAA+
Sbjct:  526 YGSRCVKPAIIFGDLSRPQPMTVDWIRYAQSLTDKTMKGMLTGPVTMLMWSFSREDVSRQVQAQQLALAIRDEVLDLERAGIKIVQIDEAAFREGLPLRKAQWQQYLDWAVAAFRLCASGVRDETQIHTHMCYSEFNDVIKSIAAMDADVITIETSRSDMELLDAF-EAFDYPNDIGPGVYDIHSPRVPETAEMVSLIAKAARRIPAERLWVNPDCGLKTRGWPETEAALINMVAAAR 762
[Close]
BLAST of CF832574 vs. ExPASy Swiss-Prot
Match: METE_MYCTU (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Mycobacterium tuberculosis GN=metE PE=3 SV=1)

HSP 1 Score: 294.278 bits (752), Expect = 4.372e-79
Identity = 143/238 (60.08%), Postives = 179/238 (75.21%), Query Frame = 1 
Query:    1 YGSPCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAK 714
            YGS CV+PPI+YGDVSRP+AMTV W + AQS+T +P+KGMLTGPVTIL WSFVR+DQP  +T  Q+ALAI+DE  DL+ AGI VIQ+DE ALRE LPLR+++ A YL WAV +FR+   GV D TQIHTH+CYS F ++I +I D+DADV +IE +RS  ++L      + +  G+GPGVYDIHSPR+PS EE+AD +   L  +    LWVNPDCGLKTR   EV  +L NMVAAA+
Sbjct:  518 YGSRCVRPPILYGDVSRPRAMTVEWITYAQSLTDKPVKGMLTGPVTILAWSFVRDDQPLADTANQVALAIRDETVDLQSAGIAVIQVDEPALRELLPLRRADQAEYLRWAVGAFRLATSGVSDATQIHTHLCYSEFGEVIGAIADLDADVTSIEAARSHMEVLDDL-NAIGFANGVGPGVYDIHSPRVPSAEEMADSLRAALRAVPAERLWVNPDCGLKTRNVDEVTASLHNMVAAAR 754
[Close]
BLAST of CF832574 vs. ExPASy Swiss-Prot
Match: METE_MYCTA (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Mycobacterium tuberculosis (strain ATCC 25177 / H37Ra) GN=metE PE=3 SV=1)

HSP 1 Score: 294.278 bits (752), Expect = 4.372e-79
Identity = 143/238 (60.08%), Postives = 179/238 (75.21%), Query Frame = 1 
Query:    1 YGSPCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAK 714
            YGS CV+PPI+YGDVSRP+AMTV W + AQS+T +P+KGMLTGPVTIL WSFVR+DQP  +T  Q+ALAI+DE  DL+ AGI VIQ+DE ALRE LPLR+++ A YL WAV +FR+   GV D TQIHTH+CYS F ++I +I D+DADV +IE +RS  ++L      + +  G+GPGVYDIHSPR+PS EE+AD +   L  +    LWVNPDCGLKTR   EV  +L NMVAAA+
Sbjct:  518 YGSRCVRPPILYGDVSRPRAMTVEWITYAQSLTDKPVKGMLTGPVTILAWSFVRDDQPLADTANQVALAIRDETVDLQSAGIAVIQVDEPALRELLPLRRADQAEYLRWAVGAFRLATSGVSDATQIHTHLCYSEFGEVIGAIADLDADVTSIEAARSHMEVLDDL-NAIGFANGVGPGVYDIHSPRVPSAEEMADSLRAALRAVPAERLWVNPDCGLKTRNVDEVTASLHNMVAAAR 754
The following BLAST results are available for this feature:
BLAST of CF832574 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 286
Match NameE-valueIdentityDescription
METE_BLOFL6.744e-8058.825-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_PASMU1.150e-7962.615-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_ACTPJ1.150e-7962.185-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_ACTP71.150e-7962.185-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_ACTP21.150e-7962.185-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_BURXL1.502e-7963.455-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_PSEU51.962e-7963.035-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_PSESM4.372e-7963.035-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_MYCTU4.372e-7960.085-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_MYCTA4.372e-7960.085-methyltetrahydropteroyltriglutamate--homocystein... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS02_02B06_r Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa02B06, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF832574 ID=CF832574; Name=CF832574; organism=Citrus sinensis; type=EST; length=716bp
TATGGATCTCCCTGTGTGAAGCCACCCATCATCTATGGTGATGTGAGCCG
TCCCAAGGCTATGACCGTCTTCTGGTCCTCCTTGGCTCAGAGCATGACAG
CTCGCCCAATGAAAGGAATGCTTACTGGCCCTGTCACCATTCTCAACTGG
TCCTTTGTTAGAAATGACCAGCCCAGACATGAAACCTGCTACCAGATTGC
TTTGGCCATCAAGGACGAAGTCGAGGATCTTGAGAAGGCAGGCATTAACG
TTATTCAAATTGATGAGGCTGCCTTGAGGGAAGGATTGCCTCTTAGGAAA
TCTGAGCATGCTTTCTACTTGGACTGGGCAGTCCACTCCTTCAGAATCAC
CAACTGTGGCGTCCAGGACACTACCCAGATCCACACCCACATGTGCTACT
CCAACTTCAACGACATTATCCACTCAATTATCGACATGGATGCTGATGTG
ATCACCATTGAGAACTCTCGTTCTGATGAGAAGCTCCTGTCTGTCTTCCG
TGAGGGAGTCAAGTATGGTGCTGGAATTGGCCCTGGTGTGTATGACATCC
ACTCTCCAAGAATACCATCAACTGAAGAAATTGCTGACAGAATCAACAAG
ATGCTTGCTGTGCTAGAAACCAACATTTTGTGGGTTAACCCTGACTGTGG
TCTTAAGACTCGCAAGTACACTGAGGTGAAGCCAGCCCTCTCAAACATGG
TCGCTGCTGCCAAGCT
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