CF832741

Overview
NameCF832741
Unique NameCF832741
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length706
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CF832741 vs. ExPASy Swiss-Prot
Match: XYLA_BACTN (Xylose isomerase OS=Bacteroides thetaiotaomicron GN=xylA PE=3 SV=1)

HSP 1 Score: 142.51 bits (358), Expect = 2.083e-33
Identity = 74/139 (53.24%), Postives = 91/139 (65.47%), Query Frame = -3
Query:  288 GNIDANTGDPQVGWDTDQFLTDIGEATLIMLSVIKNGGLAPGGFNFDAKLRRESTDVEDLFIAHIVGMDTVARGLRTAAKLIEDGSLPELVRKRYQSFDSEIGQKIEAGKADFEMLEKKAMEWGEPKVPSAKQELAEMI 704
            G+IDAN GD Q GWDTDQF  D  E T  M+ +I+NGGL  GG NFDAK RR STD+ED+FIAHI GMD +AR L +AA L+++    +++  RY SFD   G++ E GK   E +   A   GEPK  S KQEL E I
Sbjct:  295 GSIDANRGDYQNGWDTDQFPIDNYELTQAMMQIIRNGGLGTGGTNFDAKTRRNSTDLEDIFIAHIAGMDAMARALESAAALLDESPYKKMLADRYASFDGGKGKEFEDGKLTLEDVVAYAKTKGEPKQTSGKQELYEAI 433          
BLAST of CF832741 vs. ExPASy Swiss-Prot
Match: XYLA_ANATD (Xylose isomerase OS=Anaerocellum thermophilum (strain DSM 6725 / Z-1320) GN=xylA PE=3 SV=1)

HSP 1 Score: 141.739 bits (356), Expect = 3.553e-33
Identity = 72/139 (51.80%), Postives = 95/139 (68.35%), Query Frame = -3
Query:  288 GNIDANTGDPQVGWDTDQFLTDIGEATLIMLSVIKNGGLAPGGFNFDAKLRRESTDVEDLFIAHIVGMDTVARGLRTAAKLIEDGSLPELVRKRYQSFDSEIGQKIEAGKADFEMLEKKAMEWGEPKVPSAKQELAEMI 704
            G+IDAN GD  +GWDTDQF TD+   TL M  VIK GG   GG NFDAK+RR S ++EDL I HI GMD  A+G + A KL++DG   + + +RY+S+   IG KI +G+A+F+MLE+ A+   + +  S KQEL EMI
Sbjct:  292 GSIDANMGDLLLGWDTDQFPTDVRLTTLAMYEVIKAGGFDKGGLNFDAKVRRGSFELEDLVIGHIAGMDAFAKGFKIAYKLVKDGVFDKFIDERYKSYKEGIGAKIVSGEANFKMLEEYALSLDKIENKSGKQELLEMI 430          
BLAST of CF832741 vs. ExPASy Swiss-Prot
Match: XYLA_THETU (Xylose isomerase OS=Thermoanaerobacter thermosulfurogenes GN=xylA PE=1 SV=1)

HSP 1 Score: 141.354 bits (355), Expect = 4.641e-33
Identity = 73/139 (52.52%), Postives = 90/139 (64.75%), Query Frame = -3
Query:  288 GNIDANTGDPQVGWDTDQFLTDIGEATLIMLSVIKNGGLAPGGFNFDAKLRRESTDVEDLFIAHIVGMDTVARGLRTAAKLIEDGSLPELVRKRYQSFDSEIGQKIEAGKADFEMLEKKAMEWGEPKVPSAKQELAEMI 704
            G+IDANTGD  +GWDTDQF TDI   TL M  VIK GG   GG NFDAK+RR S + EDLF+ HI GMD  A+G + A KL++D    + + +RY S+   IG  I +GKADF  LEK A+E  +    S +QEL E I
Sbjct:  293 GSIDANTGDMLLGWDTDQFPTDIRMTTLAMYEVIKMGGFDKGGLNFDAKVRRASFEPEDLFLGHIAGMDAFAKGFKVAYKLVKDRVFDKFIEERYASYKDGIGADIVSGKADFRSLEKYALERSQIVNKSGRQELLESI 431          
BLAST of CF832741 vs. ExPASy Swiss-Prot
Match: XYLA_BACST (Xylose isomerase OS=Bacillus stearothermophilus GN=xylA PE=1 SV=2)

HSP 1 Score: 140.969 bits (354), Expect = 6.061e-33
Identity = 73/150 (48.67%), Postives = 99/150 (66.00%), Query Frame = -3
Query:  258 GNIDANTGDPQVGWDTDQFLTDIGEATLIMLSVIKNGGLAPGGFNFDAKLRRESTDVEDLFIAHIVGMDTVARGLRTAAKLIEDGSLPELVRKRYQSFDSEIGQKIEAGKADFEMLEKKAMEWGEPKVPSAKQE-LAEMIFQSAI*IAAA 704
            G++DAN GD  +GWDTD+F TD+   TL M  ++KNGGL  GG NFDAK+RR S + EDLF AHI GMD+ A GL+ A +LIED    E + +RY+S+   IG++I  G ADF  LE  A++ GE +  S +QE L  ++ Q  + + AA
Sbjct:  291 GSVDANQGDMLLGWDTDEFPTDLYSTTLAMYEILKNGGLGRGGLNFDAKVRRGSFEPEDLFYAHIAGMDSFAVGLKVAHRLIEDRVFDEFIEERYKSYTEGIGREIVEGTADFHKLEAHALQLGEIQNQSGRQERLKTLLNQYLLEVCAA 440          
BLAST of CF832741 vs. ExPASy Swiss-Prot
Match: XYLA_BACSK (Xylose isomerase OS=Bacillus clausii (strain KSM-K16) GN=xylA PE=3 SV=1)

HSP 1 Score: 140.584 bits (353), Expect = 7.916e-33
Identity = 69/139 (49.64%), Postives = 91/139 (65.47%), Query Frame = -3
Query:  288 GNIDANTGDPQVGWDTDQFLTDIGEATLIMLSVIKNGGLAPGGFNFDAKLRRESTDVEDLFIAHIVGMDTVARGLRTAAKLIEDGSLPELVRKRYQSFDSEIGQKIEAGKADFEMLEKKAMEWGEPKVPSAKQELAEMI 704
            G++DAN GDP +GWDTD+F TD+   TL M  +++NGGL  GG NFDAK+RR S + +DLF AHI GMD+ A GL+ A +LI+D  L + V  RYQS+   IG++I  G  DF  LE  A+  GE K  S + E  + I
Sbjct:  291 GSVDANQGDPLLGWDTDEFPTDLYSTTLAMYEILQNGGLGRGGLNFDAKVRRGSFEADDLFFAHIAGMDSFAIGLKVAQQLIDDRVLEQFVDSRYQSYKEGIGREIVLGNTDFHQLEAHALSLGEIKTRSGRVERIKAI 429          
BLAST of CF832741 vs. ExPASy Swiss-Prot
Match: XYLA_RUMFL (Xylose isomerase OS=Ruminococcus flavefaciens GN=xylA PE=3 SV=2)

HSP 1 Score: 139.428 bits (350), Expect = 1.764e-32
Identity = 75/146 (51.37%), Postives = 94/146 (64.38%), Query Frame = -3
Query:  273 GNIDANTGDPQVGWDTDQFLTDIGEATLIMLSVIKNGGLAPGGFNFDAKLRRESTDVEDLFIAHIVGMDTVARGLRTAAKLIEDGSLPELVRKRYQSFDSEIGQKIEAGKADFEMLEKKAMEWGE--PKVPSAKQELAEMIFQSAI 704
            G+IDAN GD  +GWDTDQF T+I + T+ M  VIK GG   GG NFDAK RR S   ED+F ++I GMD  A G R A KLIEDG + + V  RY S+++ IG  I AGKADF  LEK A+E GE    + S +QE+ E I  + +
Sbjct:  290 GSIDANQGDVLLGWDTDQFPTNIYDTTMCMYEVIKAGGFTNGGLNFDAKARRGSFTPEDIFYSYIAGMDAFALGFRAALKLIEDGRIDKFVADRYASWNTGIGADIIAGKADFASLEKYALEKGEVTASLSSGRQEMLESIVNNVL 435          
BLAST of CF832741 vs. ExPASy Swiss-Prot
Match: XYLA_PECCP (Xylose isomerase OS=Pectobacterium carotovorum subsp. carotovorum (strain PC1) GN=xylA PE=3 SV=1)

HSP 1 Score: 139.428 bits (350), Expect = 1.764e-32
Identity = 73/138 (52.90%), Postives = 92/138 (66.67%), Query Frame = -3
Query:  294 GNIDANTGDPQVGWDTDQFLTDIGEATLIMLSVIKNGGLAPGGFNFDAKLRRESTDVEDLFIAHIVGMDTVARGLRTAAKLIEDGSLPELVRKRYQSFDSEIGQKIEAGKADFEMLEKKAMEWG-EPKVPSAKQELAE 704
            G++DAN GDPQ+GWDTDQF   + E TLIM  ++K GG   GG NFDAK+RR+STD  DLF AHI  MDT+A  L+ AA++IED  L +LV KRY  ++ E+GQ+I  GKA  E L   A      P+  S +QEL E
Sbjct:  293 GSVDANRGDPQLGWDTDQFPNSVEENTLIMYEILKAGGFTTGGLNFDAKVRRQSTDRYDLFHAHIGAMDTMALALKAAARMIEDDKLNQLVAKRYAGWNGELGQQILQGKASLESLAHYAESHQLAPQHQSGQQELLE 430          
BLAST of CF832741 vs. ExPASy Swiss-Prot
Match: XYLA_BACSW (Xylose isomerase OS=Bacillus sp. (strain LW2) GN=xylA PE=1 SV=1)

HSP 1 Score: 139.428 bits (350), Expect = 1.764e-32
Identity = 72/150 (48.00%), Postives = 98/150 (65.33%), Query Frame = -3
Query:  258 GNIDANTGDPQVGWDTDQFLTDIGEATLIMLSVIKNGGLAPGGFNFDAKLRRESTDVEDLFIAHIVGMDTVARGLRTAAKLIEDGSLPELVRKRYQSFDSEIGQKIEAGKADFEMLEKKAMEWGEPKVPSAKQE-LAEMIFQSAI*IAAA 704
            G++DAN GD  +GWDTD+F TD+   TL M  ++KNGGL  GG NFDAK+RR S + EDLF AHI GMD+ A GL+ A +LIED    E + +RY+S+   IG++I  G  DF  LE  A++ GE +  S +QE L  ++ Q  + + AA
Sbjct:  291 GSVDANQGDMLLGWDTDEFPTDLYSTTLAMYEILKNGGLGRGGLNFDAKVRRGSFEPEDLFYAHIAGMDSFAVGLKVAHRLIEDRVFDEFIEERYKSYTEGIGREIVEGTVDFHKLEAHALQLGEIQNQSGRQERLKTLLNQYLLEVCAA 440          
BLAST of CF832741 vs. ExPASy Swiss-Prot
Match: XYLA_ERWCT (Xylose isomerase OS=Erwinia carotovora subsp. atroseptica GN=xylA PE=3 SV=1)

HSP 1 Score: 138.272 bits (347), Expect = 3.929e-32
Identity = 72/138 (52.17%), Postives = 92/138 (66.67%), Query Frame = -3
Query:  294 GNIDANTGDPQVGWDTDQFLTDIGEATLIMLSVIKNGGLAPGGFNFDAKLRRESTDVEDLFIAHIVGMDTVARGLRTAAKLIEDGSLPELVRKRYQSFDSEIGQKIEAGKADFEMLEKKAMEWG-EPKVPSAKQELAE 704
            G++DAN GDPQ+GWDTDQF   + E TLIM  ++K GG   GG NFDAK+RR+STD  DLF AHI  MDT+A  L+ AA++IED  L +LV KRY  ++ E+GQ+I  G A  E L + A      P+  S +QEL E
Sbjct:  293 GSVDANRGDPQLGWDTDQFPNSVEENTLIMYEILKAGGFTTGGLNFDAKVRRQSTDRYDLFHAHIGAMDTMALALKAAARMIEDDKLNQLVAKRYAGWNGELGQQILQGNASLESLAQYAESHQLAPQHQSGQQELLE 430          
BLAST of CF832741 vs. ExPASy Swiss-Prot
Match: XYLA_ACTPJ (Xylose isomerase OS=Actinobacillus pleuropneumoniae serotype 3 (strain JL03) GN=xylA PE=3 SV=1)

HSP 1 Score: 138.272 bits (347), Expect = 3.929e-32
Identity = 72/148 (48.65%), Postives = 99/148 (66.89%), Query Frame = -3
Query:  273 GNIDANTGDPQVGWDTDQFLTDIGEATLIMLSVIKNGGLAPGGFNFDAKLRRESTDVEDLFIAHIVGMDTVARGLRTAAKLIEDGSLPELVRKRYQSFDSEIGQKIEAGKADFE----MLEKKAMEWGEPKVPSAKQELAEMIFQSAI 704
            G+IDAN GDPQ+GWDTDQF   + E TL+M  ++K GG   GGFNFDAK+RR+STD  DLF  HI  +D +A  L+ AAK+IED +L  +V +RY  ++ ++GQ+I AGKA  E    ++E KA+   +PK  S +QE  E +  + I
Sbjct:  293 GSIDANRGDPQLGWDTDQFPNSVEENTLVMYEILKAGGFTTGGFNFDAKIRRQSTDPYDLFHGHIGAIDVLALSLKRAAKMIEDKTLQGIVDQRYAGWNGDLGQQILAGKASLEDLAKIVESKAL---DPKPVSGQQEYLENLVNNYI 437          
The following BLAST results are available for this feature:
BLAST of CF832741 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 172
Match NameE-valueIdentityDescription
XYLA_BACTN2.083e-3353.24Xylose isomerase OS=Bacteroides thetaiotaomicron G... [more]
XYLA_ANATD3.553e-3351.80Xylose isomerase OS=Anaerocellum thermophilum (str... [more]
XYLA_THETU4.641e-3352.52Xylose isomerase OS=Thermoanaerobacter thermosulfu... [more]
XYLA_BACST6.061e-3348.67Xylose isomerase OS=Bacillus stearothermophilus GN... [more]
XYLA_BACSK7.916e-3349.64Xylose isomerase OS=Bacillus clausii (strain KSM-K... [more]
XYLA_RUMFL1.764e-3251.37Xylose isomerase OS=Ruminococcus flavefaciens GN=x... [more]
XYLA_PECCP1.764e-3252.90Xylose isomerase OS=Pectobacterium carotovorum sub... [more]
XYLA_BACSW1.764e-3248.00Xylose isomerase OS=Bacillus sp. (strain LW2) GN=x... [more]
XYLA_ERWCT3.929e-3252.17Xylose isomerase OS=Erwinia carotovora subsp. atro... [more]
XYLA_ACTPJ3.929e-3248.65Xylose isomerase OS=Actinobacillus pleuropneumonia... [more]

Pages

back to top
Properties
Property NameValue
Genbank descriptionUCRCS02_02F15_r Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa02F15, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF832741 ID=CF832741; Name=CF832741; organism=Citrus sinensis; type=EST; length=706bp
CTTTTTTTTTTTTCCAAATTGAACTTCGATCATTATTAAAGCAATGTTAT
TGACAAAAGATTTCAAGATCGTAGGATTCAAGTTTTATAACAGCTGTCAT
ATCGATAACTAAACAAGTTCTTTATAAAGCCTAAGCTAGACAAACATGTC
AAAATTTCCCCGCATGAAAAGACCCATTTCCCCAAGAGGGTTCACTGACT
GGAGACAAACATAACTTCAATTTGCCCCTCTCTCTTTATTTGTAAGTTCT
GCTTCGGAGCAGCCGCTATCTATATTGCAGATTGGAAGATCATTTCTGCA
AGTTCCTGCTTAGCAGAGGGAACCTTGGGTTCTCCCCATTCCATGGCCTT
CTTCTCAAGCATTTCAAAATCAGCCTTACCAGCCTCTATTTTTTGCCCAA
TCTCTGAGTCAAAGCTCTGGTATCGCTTGCGGACAAGCTCCGGTAGAGAA
CCATCCTCAATCAGCTTGGCAGCAGTTCGAAGTCCACGAGCAACGGTATC
CATTCCAACAATATGAGCAATGAACAAGTCTTCAACGTCAGTGCTCTCTC
TTCGTAATTTTGCATCAAAGTTGAATCCTCCAGGTGCCAAACCTCCATTT
TTAATCACACTGAGCATAATCAAAGTAGCCTCTCCAATATCTGTCAGGAA
TTGATCTGTATCCCAACCAACTTGAGGGTCACCAGTGTTTGCATCGATGT
TTCCTA
back to top