CF832741

Overview
NameCF832741
Unique NameCF832741
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length706
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CF832741 vs. ExPASy Swiss-Prot
Match: XYLA_SERP5 (Xylose isomerase OS=Serratia proteamaculans (strain 568) GN=xylA PE=3 SV=1)

HSP 1 Score: 132.109 bits (331), Expect = 2.816e-30
Identity = 67/138 (48.55%), Postives = 88/138 (63.77%), Query Frame = -3
Query:  294 GNIDANTGDPQVGWDTDQFLTDIGEATLIMLSVIKNGGLAPGGFNFDAKLRRESTDVEDLFIAHIVGMDTVARGLRTAAKLIEDGSLPELVRKRYQSFDSEIGQKIEAGKADFEMLEKKAMEWG-EPKVPSAKQELAE 704
            G++DAN GDPQ+GWDTDQF   + E  L+M  ++K GG   GG NFDAK+RR+STD  DLF  HI  MDT+A  L+ AAK++ DG L + V KRY  ++ E+GQ+I  GK   E L + A      P+  S +QE  E
Sbjct:  293 GSVDANRGDPQLGWDTDQFPISVEENALVMFEILKAGGFTTGGLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKVAAKMVTDGQLHQQVSKRYAGWNGELGQQILQGKLSLEALAQYAEGHALAPQHVSGRQEQLE 430          
BLAST of CF832741 vs. ExPASy Swiss-Prot
Match: XYLA_MANSM (Xylose isomerase OS=Mannheimia succiniciproducens (strain MBEL55E) GN=xylA PE=3 SV=1)

HSP 1 Score: 131.724 bits (330), Expect = 3.677e-30
Identity = 67/144 (46.53%), Postives = 93/144 (64.58%), Query Frame = -3
Query:  273 GNIDANTGDPQVGWDTDQFLTDIGEATLIMLSVIKNGGLAPGGFNFDAKLRRESTDVEDLFIAHIVGMDTVARGLRTAAKLIEDGSLPELVRKRYQSFDSEIGQKIEAGKADFEMLEKKAMEWGEPKVPSAKQELAEMIFQSAI 704
            G+IDAN GDPQ+GWDTDQF   + E TL +  ++K GGL  GGFNFDAK+RR+S +  DLF  HI  +D +A  L+ AAK++ED +L  +V +RY  ++ E+GQ+I AGK+  E L + A    +P   S +QE  E +    I
Sbjct:  293 GSIDANRGDPQLGWDTDQFPNSVEENTLAIYEILKAGGLTTGGFNFDAKIRRQSINPYDLFHGHIGAIDVLALSLKRAAKMVEDHTLQNIVDQRYAGWNGELGQQILAGKSSLEALAQAAQNL-DPNPVSGQQEYIENLVNGYI 435          
BLAST of CF832741 vs. ExPASy Swiss-Prot
Match: XYLA_ESCF3 (Xylose isomerase OS=Escherichia fergusonii (strain ATCC 35469 / DSM 13698 / CDC 0568-73) GN=xylA PE=3 SV=1)

HSP 1 Score: 131.724 bits (330), Expect = 3.677e-30
Identity = 67/140 (47.86%), Postives = 89/140 (63.57%), Query Frame = -3
Query:  288 GNIDANTGDPQVGWDTDQFLTDIGEATLIMLSVIKNGGLAPGGFNFDAKLRRESTDVEDLFIAHIVGMDTVARGLRTAAKLIEDGSLPELVRKRYQSFDSEIGQKIEAGKADFEMLEKKAMEWG-EPKVPSAKQELAEMI 704
            G++DAN GD Q+GWDTDQF   + E  L+M  ++K GG   GG NFDAK+RR+STD  DLF  HI  MDT+A  L+ AA++IEDG L + V +RY  ++SE+GQ+I  G+     L K A +    P   S  QEL E +
Sbjct:  293 GSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTGGLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKVAARMIEDGELDKRVAQRYSGWNSELGQQILKGQMSLSDLAKYAQDHNLSPVHQSGHQELLESL 432          
BLAST of CF832741 vs. ExPASy Swiss-Prot
Match: XYLA_BACHD (Xylose isomerase OS=Bacillus halodurans GN=xylA PE=3 SV=1)

HSP 1 Score: 131.339 bits (329), Expect = 4.803e-30
Identity = 66/134 (49.25%), Postives = 88/134 (65.67%), Query Frame = -3
Query:  303 GNIDANTGDPQVGWDTDQFLTDIGEATLIMLSVIKNGGLAPGGFNFDAKLRRESTDVEDLFIAHIVGMDTVARGLRTAAKLIEDGSLPELVRKRYQSFDSEIGQKIEAGKADFEMLEKKAMEWGEPKVPSAKQE 704
            G++DAN GD  +GWDTD+F TD+   TL M  ++KN GL  GG NFDAK+RR S +  DLF AHI GMD+ A GL+ A +L+ED  L +++  RY+S+ S IGQKI     + + LE  A+  GE K  S +QE
Sbjct:  291 GSVDANQGDTLLGWDTDEFPTDLYTTTLAMYEILKNDGLGKGGLNFDAKVRRGSFEANDLFHAHIAGMDSFAIGLKVANQLLEDRVLEDVIDNRYKSYQSGIGQKIANNDTNLKELEAYALSLGEIKHSSGQQE 424          
BLAST of CF832741 vs. ExPASy Swiss-Prot
Match: XYLA_XANC5 (Xylose isomerase OS=Xanthomonas campestris pv. vesicatoria (strain 85-10) GN=xylA1 PE=3 SV=1)

HSP 1 Score: 130.568 bits (327), Expect = 8.192e-30
Identity = 71/137 (51.82%), Postives = 86/137 (62.77%), Query Frame = -3
Query:  294 GNIDANTGDPQVGWDTDQFLTDIGEATLIMLSVIKNGGLAPGGFNFDAKLRRESTDVEDLFIAHIVGMDTVARGLRTAAKLIEDGSLPELVRKRYQSFDSEIGQKIEAGKADFEMLEKKAMEWGEPKVPSAKQELAE 704
            G+IDAN G+PQ GWDTDQF TD+ +    ML V++ GGLAPGG NFDAK+RRES+D +DLF+AHI GMD  ARGL  A  L+    L +   +RY SFDS  G    AGK     L K A     P+  S +QE  E
Sbjct:  301 GSIDANRGNPQNGWDTDQFPTDLYDTVGAMLVVLRQGGLAPGGLNFDAKVRRESSDPQDLFLAHIGGMDAFARGLEVANALLTASPLEQWRAERYASFDSGAGADFAAGKTTLADLAKHAAS-NAPQQLSGRQEAYE 436          
BLAST of CF832741 vs. ExPASy Swiss-Prot
Match: XYLA_LACPE (Xylose isomerase OS=Lactobacillus pentosus GN=xylA PE=3 SV=1)

HSP 1 Score: 130.568 bits (327), Expect = 8.192e-30
Identity = 65/123 (52.85%), Postives = 84/123 (68.29%), Query Frame = -3
Query:  339 GNIDANTGDPQVGWDTDQFLTDIGEATLIMLSVIKNGGLAP-GGFNFDAKLRRESTDVEDLFIAHIVGMDTVARGLRTAAKLIEDGSLPELVRKRYQSFDSEIGQKIEAGKADFEMLEKKAME 704
            G++DAN GD  +GWD D+F +D+ EAT  M  V++NG + P GG NFDAK RR S    DLF  HIVG+DT A GLR A K+ +DG L +LV  RY S+ S +G +IEAG ADF+ LE  A++
Sbjct:  295 GSLDANQGDKLIGWDIDEFPSDLYEATAAMYEVVENGSIGPRGGLNFDAKPRRSSFAANDLFYGHIVGIDTFAAGLRVALKMKQDGFLEKLVADRYSSYQSGVGAEIEAGTADFKSLESYAID 417          
BLAST of CF832741 vs. ExPASy Swiss-Prot
Match: XYLA_BURPP (Xylose isomerase OS=Burkholderia phytofirmans (strain DSM 17436 / PsJN) GN=xylA PE=3 SV=1)

HSP 1 Score: 130.568 bits (327), Expect = 8.192e-30
Identity = 67/145 (46.21%), Postives = 89/145 (61.38%), Query Frame = -3
Query:  273 GNIDANTGDPQVGWDTDQFLTDIGEATLIMLSVIKNGGLAPGGFNFDAKLRRESTDVEDLFIAHIVGMDTVARGLRTAAKLIEDGSLPELVRKRYQSFDSEIGQKIEAGKADFEMLEKKAMEWG-EPKVPSAKQELAEMIFQSAI 704
            G++DAN GDPQ GWDTDQF   + E TL    ++K+GG   GG NFD+K+RR+S D EDLF  HI  +D +A  +  AA LIE+  L +  R+RY  +D+E G+KI +G      L ++AM  G  P+  S  QEL E I   AI
Sbjct:  292 GSVDANRGDPQNGWDTDQFPNSVEELTLAFYEILKHGGFTTGGMNFDSKVRRQSVDPEDLFYGHIGAIDNLALAVERAAVLIENDRLDQFKRQRYSGWDAEFGRKISSGDYSLSALAEEAMARGLNPQHASGHQELMENIVNQAI 436          
BLAST of CF832741 vs. ExPASy Swiss-Prot
Match: XYLA_SHISS (Xylose isomerase OS=Shigella sonnei (strain Ss046) GN=xylA PE=3 SV=1)

HSP 1 Score: 130.183 bits (326), Expect = 1.070e-29
Identity = 66/138 (47.83%), Postives = 88/138 (63.77%), Query Frame = -3
Query:  294 GNIDANTGDPQVGWDTDQFLTDIGEATLIMLSVIKNGGLAPGGFNFDAKLRRESTDVEDLFIAHIVGMDTVARGLRTAAKLIEDGSLPELVRKRYQSFDSEIGQKIEAGKADFEMLEKKAMEWG-EPKVPSAKQELAE 704
            G++DAN GD Q+GWDTDQF   + E  L+M  ++K GG   GG NFDAK+RR+STD  DLF  HI  MDT+A  L+ AA++IEDG L + + +RY  ++SE+GQ+I  G+     L K A E    P   S +QE  E
Sbjct:  293 GSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTGGLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNSELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLE 430          
BLAST of CF832741 vs. ExPASy Swiss-Prot
Match: XYLA_SHIB3 (Xylose isomerase OS=Shigella boydii serotype 18 (strain CDC 3083-94 / BS512) GN=xylA PE=3 SV=1)

HSP 1 Score: 130.183 bits (326), Expect = 1.070e-29
Identity = 66/138 (47.83%), Postives = 88/138 (63.77%), Query Frame = -3
Query:  294 GNIDANTGDPQVGWDTDQFLTDIGEATLIMLSVIKNGGLAPGGFNFDAKLRRESTDVEDLFIAHIVGMDTVARGLRTAAKLIEDGSLPELVRKRYQSFDSEIGQKIEAGKADFEMLEKKAMEWG-EPKVPSAKQELAE 704
            G++DAN GD Q+GWDTDQF   + E  L+M  ++K GG   GG NFDAK+RR+STD  DLF  HI  MDT+A  L+ AA++IEDG L + + +RY  ++SE+GQ+I  G+     L K A E    P   S +QE  E
Sbjct:  293 GSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTGGLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNSELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLE 430          
BLAST of CF832741 vs. ExPASy Swiss-Prot
Match: XYLA_ECOSM (Xylose isomerase OS=Escherichia coli (strain SMS-3-5 / SECEC) GN=xylA PE=3 SV=1)

HSP 1 Score: 130.183 bits (326), Expect = 1.070e-29
Identity = 66/138 (47.83%), Postives = 88/138 (63.77%), Query Frame = -3
Query:  294 GNIDANTGDPQVGWDTDQFLTDIGEATLIMLSVIKNGGLAPGGFNFDAKLRRESTDVEDLFIAHIVGMDTVARGLRTAAKLIEDGSLPELVRKRYQSFDSEIGQKIEAGKADFEMLEKKAMEWG-EPKVPSAKQELAE 704
            G++DAN GD Q+GWDTDQF   + E  L+M  ++K GG   GG NFDAK+RR+STD  DLF  HI  MDT+A  L+ AA++IEDG L + + +RY  ++SE+GQ+I  G+     L K A E    P   S +QE  E
Sbjct:  293 GSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTGGLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNSELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLE 430          
The following BLAST results are available for this feature:
BLAST of CF832741 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 172
Match NameE-valueIdentityDescription
XYLA_SERP52.816e-3048.55Xylose isomerase OS=Serratia proteamaculans (strai... [more]
XYLA_MANSM3.677e-3046.53Xylose isomerase OS=Mannheimia succiniciproducens ... [more]
XYLA_ESCF33.677e-3047.86Xylose isomerase OS=Escherichia fergusonii (strain... [more]
XYLA_BACHD4.803e-3049.25Xylose isomerase OS=Bacillus halodurans GN=xylA PE... [more]
XYLA_XANC58.192e-3051.82Xylose isomerase OS=Xanthomonas campestris pv. ves... [more]
XYLA_LACPE8.192e-3052.85Xylose isomerase OS=Lactobacillus pentosus GN=xylA... [more]
XYLA_BURPP8.192e-3046.21Xylose isomerase OS=Burkholderia phytofirmans (str... [more]
XYLA_SHISS1.070e-2947.83Xylose isomerase OS=Shigella sonnei (strain Ss046)... [more]
XYLA_SHIB31.070e-2947.83Xylose isomerase OS=Shigella boydii serotype 18 (s... [more]
XYLA_ECOSM1.070e-2947.83Xylose isomerase OS=Escherichia coli (strain SMS-3... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS02_02F15_r Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa02F15, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF832741 ID=CF832741; Name=CF832741; organism=Citrus sinensis; type=EST; length=706bp
CTTTTTTTTTTTTCCAAATTGAACTTCGATCATTATTAAAGCAATGTTAT
TGACAAAAGATTTCAAGATCGTAGGATTCAAGTTTTATAACAGCTGTCAT
ATCGATAACTAAACAAGTTCTTTATAAAGCCTAAGCTAGACAAACATGTC
AAAATTTCCCCGCATGAAAAGACCCATTTCCCCAAGAGGGTTCACTGACT
GGAGACAAACATAACTTCAATTTGCCCCTCTCTCTTTATTTGTAAGTTCT
GCTTCGGAGCAGCCGCTATCTATATTGCAGATTGGAAGATCATTTCTGCA
AGTTCCTGCTTAGCAGAGGGAACCTTGGGTTCTCCCCATTCCATGGCCTT
CTTCTCAAGCATTTCAAAATCAGCCTTACCAGCCTCTATTTTTTGCCCAA
TCTCTGAGTCAAAGCTCTGGTATCGCTTGCGGACAAGCTCCGGTAGAGAA
CCATCCTCAATCAGCTTGGCAGCAGTTCGAAGTCCACGAGCAACGGTATC
CATTCCAACAATATGAGCAATGAACAAGTCTTCAACGTCAGTGCTCTCTC
TTCGTAATTTTGCATCAAAGTTGAATCCTCCAGGTGCCAAACCTCCATTT
TTAATCACACTGAGCATAATCAAAGTAGCCTCTCCAATATCTGTCAGGAA
TTGATCTGTATCCCAACCAACTTGAGGGTCACCAGTGTTTGCATCGATGT
TTCCTA
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