CF832747

Overview
NameCF832747
Unique NameCF832747
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length646
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CF832747 vs. ExPASy Swiss-Prot
Match: UB2D2_PIG (Ubiquitin-conjugating enzyme E2 D2 OS=Sus scrofa GN=UBE2D2 PE=2 SV=1)

HSP 1 Score: 243.432 bits (620), Expect = 7.341e-64
Identity = 109/138 (78.99%), Postives = 120/138 (86.96%), Query Frame = -3 
Query:  231 LKDLQKDPPTSCSAGPVAEDMFHWQATIMGPPDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYEATARSWTQKYAM 644
            L DL +DPP  CSAGPV +DMFHWQATIMGP DSPY GGVF +TIHFP DYPFKPPKVAF T+++HPNINSNGSICLDIL+ QWSPALTISKVLLSICSLL DPNPDDPLVPEIA +YK+D+ KY   +R WTQKYAM
Sbjct:   10 LNDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDRDKYNRISREWTQKYAM 147
[Close]
BLAST of CF832747 vs. ExPASy Swiss-Prot
Match: UB2D3_BOVIN (Ubiquitin-conjugating enzyme E2 D3 OS=Bos taurus GN=UBE2D3 PE=2 SV=1)

HSP 1 Score: 240.736 bits (613), Expect = 4.758e-63
Identity = 108/138 (78.26%), Postives = 119/138 (86.23%), Query Frame = -3 
Query:  231 LKDLQKDPPTSCSAGPVAEDMFHWQATIMGPPDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYEATARSWTQKYAM 644
            L DL +DPP  CSAGPV +DMFHWQATIMGP DSPY GGVF +TIHFP DYPFKPPKVAF T+++HPNINSN SICLDIL+ QWSPALTISKVLLSICSLL DPNPDDPLVPEIA +YK+D+ KY   +R WTQKYAM
Sbjct:   10 LSDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNDSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDRDKYNRISREWTQKYAM 147
[Close]
BLAST of CF832747 vs. ExPASy Swiss-Prot
Match: UBC12_ARATH (Probable ubiquitin-conjugating enzyme E2 12 OS=Arabidopsis thaliana GN=UBC12 PE=2 SV=1)

HSP 1 Score: 239.965 bits (611), Expect = 8.116e-63
Identity = 111/140 (79.29%), Postives = 126/140 (90.00%), Query Frame = -3 
Query:  228 LKDLQKDPPTSCSAGPVAE-DMFHWQATIMGPPDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYEATARSWTQKYAMG 644
            L+D+Q+ PP +CSAGPVAE D+FHWQATIMGP DSPY+GGVF V+I F  DYPFKPPKV F+TKV+HPNI+S GSICLDILKEQWSPA T SKVLLSICSLLTDPNP+DPLVPEIAH+YK DK+KYE+TA+ WTQKYAMG
Sbjct:   10 LRDMQRHPPANCSAGPVAEEDIFHWQATIMGPHDSPYSGGVFTVSIDFSSDYPFKPPKVNFKTKVYHPNIDSKGSICLDILKEQWSPAPTTSKVLLSICSLLTDPNPNDPLVPEIAHLYKVDKSKYESTAQKWTQKYAMG 149
[Close]
BLAST of CF832747 vs. ExPASy Swiss-Prot
Match: U2D2B_RAT (Ubiquitin-conjugating enzyme E2 D2B OS=Rattus norvegicus GN=Ube2d2b PE=2 SV=1)

HSP 1 Score: 234.958 bits (598), Expect = 2.611e-61
Identity = 106/138 (76.81%), Postives = 117/138 (84.78%), Query Frame = -3 
Query:  231 LKDLQKDPPTSCSAGPVAEDMFHWQATIMGPPDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYEATARSWTQKYAM 644
            L DL +DPP  CSAGPV EDMFHWQATIMGP DSPY GG F +TI FP +YPFKPPKV F T+++HPN+NSNGSICLDIL+ QWSPALTISKVLLSI SLL DPNPDDPLVPEIA +YK+D+ KY  TAR WTQKYAM
Sbjct:   10 LNDLAQDPPAQCSAGPVGEDMFHWQATIMGPNDSPYQGGAFFLTIDFPTEYPFKPPKVEFTTRIYHPNVNSNGSICLDILRSQWSPALTISKVLLSISSLLCDPNPDDPLVPEIAQIYKTDRDKYNRTAREWTQKYAM 147
[Close]
BLAST of CF832747 vs. ExPASy Swiss-Prot
Match: U2D2B_MOUSE (Ubiquitin-conjugating enzyme E2 D2B OS=Mus musculus GN=Ube2d2b PE=2 SV=1)

HSP 1 Score: 234.958 bits (598), Expect = 2.611e-61
Identity = 106/138 (76.81%), Postives = 117/138 (84.78%), Query Frame = -3 
Query:  231 LKDLQKDPPTSCSAGPVAEDMFHWQATIMGPPDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYEATARSWTQKYAM 644
            L DL +DPP  CSAGPV EDMFHWQATIMGP DSPY GG F +TI FP +YPFKPPKV F T+++HPN+NSNGSICLDIL+ QWSPALTISKVLLSI SLL DPNPDDPLVPEIA +YK+D+ KY  TAR WTQKYAM
Sbjct:   10 LNDLAQDPPAQCSAGPVGEDMFHWQATIMGPNDSPYQGGAFFLTIDFPTEYPFKPPKVEFTTRIYHPNVNSNGSICLDILRSQWSPALTISKVLLSISSLLCDPNPDDPLVPEIAQIYKTDRDKYNRTAREWTQKYAM 147
[Close]
BLAST of CF832747 vs. ExPASy Swiss-Prot
Match: UB2E2_MOUSE (Ubiquitin-conjugating enzyme E2 E2 OS=Mus musculus GN=Ube2e2 PE=2 SV=1)

HSP 1 Score: 189.504 bits (480), Expect = 1.258e-47
Identity = 85/137 (62.04%), Postives = 108/137 (78.83%), Query Frame = -3 
Query:  234 LKDLQKDPPTSCSAGPVAEDMFHWQATIMGPPDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYEATARSWTQKYA 644
            L ++  DPP +CSAGP  ++++ W++TI+GPP S Y GGVF + I F PDYPFKPPKV FRT+++H NINS G ICLDILK+ WSPALTISKVLLSICSLLTD NP DPLV  IA  Y +++A+++  AR WT++YA
Sbjct:   64 LAEITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDITFSPDYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQYMTNRAEHDRMARQWTKRYA 200
[Close]
BLAST of CF832747 vs. ExPASy Swiss-Prot
Match: UB2E2_HUMAN (Ubiquitin-conjugating enzyme E2 E2 OS=Homo sapiens GN=UBE2E2 PE=1 SV=1)

HSP 1 Score: 189.504 bits (480), Expect = 1.258e-47
Identity = 85/137 (62.04%), Postives = 108/137 (78.83%), Query Frame = -3 
Query:  234 LKDLQKDPPTSCSAGPVAEDMFHWQATIMGPPDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYEATARSWTQKYA 644
            L ++  DPP +CSAGP  ++++ W++TI+GPP S Y GGVF + I F PDYPFKPPKV FRT+++H NINS G ICLDILK+ WSPALTISKVLLSICSLLTD NP DPLV  IA  Y +++A+++  AR WT++YA
Sbjct:   64 LAEITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDITFSPDYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQYMTNRAEHDRMARQWTKRYA 200
[Close]
BLAST of CF832747 vs. ExPASy Swiss-Prot
Match: UB2E1_MOUSE (Ubiquitin-conjugating enzyme E2 E1 OS=Mus musculus GN=Ube2e1 PE=2 SV=1)

HSP 1 Score: 189.504 bits (480), Expect = 1.258e-47
Identity = 85/137 (62.04%), Postives = 108/137 (78.83%), Query Frame = -3 
Query:  234 LKDLQKDPPTSCSAGPVAEDMFHWQATIMGPPDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYEATARSWTQKYA 644
            L D+  DPP +CSAGP  ++++ W++TI+GPP S Y GGVF + I F P+YPFKPPKV FRT+++H NINS G ICLDILK+ WSPALTISKVLLSICSLLTD NP DPLV  IA  Y +++A+++  AR WT++YA
Sbjct:   56 LADITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDITFTPEYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQYMTNRAEHDRMARQWTKRYA 192
[Close]
BLAST of CF832747 vs. ExPASy Swiss-Prot
Match: UB2E1_HUMAN (Ubiquitin-conjugating enzyme E2 E1 OS=Homo sapiens GN=UBE2E1 PE=1 SV=1)

HSP 1 Score: 189.504 bits (480), Expect = 1.258e-47
Identity = 85/137 (62.04%), Postives = 108/137 (78.83%), Query Frame = -3 
Query:  234 LKDLQKDPPTSCSAGPVAEDMFHWQATIMGPPDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYEATARSWTQKYA 644
            L D+  DPP +CSAGP  ++++ W++TI+GPP S Y GGVF + I F P+YPFKPPKV FRT+++H NINS G ICLDILK+ WSPALTISKVLLSICSLLTD NP DPLV  IA  Y +++A+++  AR WT++YA
Sbjct:   56 LADITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDITFTPEYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQYMTNRAEHDRMARQWTKRYA 192
[Close]
BLAST of CF832747 vs. ExPASy Swiss-Prot
Match: UBCD2_DROME (Ubiquitin-conjugating enzyme E2-24 kDa OS=Drosophila melanogaster GN=UbcD2 PE=1 SV=1)

HSP 1 Score: 187.578 bits (475), Expect = 4.780e-47
Identity = 84/137 (61.31%), Postives = 106/137 (77.37%), Query Frame = -3 
Query:  234 LKDLQKDPPTSCSAGPVAEDMFHWQATIMGPPDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYEATARSWTQKYA 644
            L ++  DPP +CSAGP  ++++ W +TI+GPP S Y GGVF + IHF P+YPFKPPKV FRT+++H NINS G ICLDILK+ WSPALTISKVLLSICSLLTD NP DPLV  IA  Y  ++ +++  AR WT++YA
Sbjct:   95 LAEITLDPPPNCSAGPKGDNLYEWVSTILGPPGSVYEGGVFFLDIHFSPEYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQYLQNREEHDRIARLWTKRYA 231
The following BLAST results are available for this feature:
BLAST of CF832747 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 263
Match NameE-valueIdentityDescription
UB2D2_PIG7.341e-6478.99Ubiquitin-conjugating enzyme E2 D2 OS=Sus scrofa G... [more]
UB2D3_BOVIN4.758e-6378.26Ubiquitin-conjugating enzyme E2 D3 OS=Bos taurus G... [more]
UBC12_ARATH8.116e-6379.29Probable ubiquitin-conjugating enzyme E2 12 OS=Ara... [more]
U2D2B_RAT2.611e-6176.81Ubiquitin-conjugating enzyme E2 D2B OS=Rattus norv... [more]
U2D2B_MOUSE2.611e-6176.81Ubiquitin-conjugating enzyme E2 D2B OS=Mus musculu... [more]
UB2E2_MOUSE1.258e-4762.04Ubiquitin-conjugating enzyme E2 E2 OS=Mus musculus... [more]
UB2E2_HUMAN1.258e-4762.04Ubiquitin-conjugating enzyme E2 E2 OS=Homo sapiens... [more]
UB2E1_MOUSE1.258e-4762.04Ubiquitin-conjugating enzyme E2 E1 OS=Mus musculus... [more]
UB2E1_HUMAN1.258e-4762.04Ubiquitin-conjugating enzyme E2 E1 OS=Homo sapiens... [more]
UBCD2_DROME4.780e-4761.31Ubiquitin-conjugating enzyme E2-24 kDa OS=Drosophi... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS02_02F23_r Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa02F23, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF832747 ID=CF832747; Name=CF832747; organism=Citrus sinensis; type=EST; length=646bp
TTTTTTTTTTTCTAGTGAAATAAACTACATTGAATGATAGGAGTTTCAAA
CAAGAAACATTCAACATTAACAAAAACGTCTTGCCCTTGCAACTCCTCCC
CAAACATCCTTCCTCATTACTAAAATACAAAACATCCCCTTCCCGAAGCA
AGATTGAAGAAAAAGGAGAAAAAAACACAACATATTTCATATAAATGAGT
TCCATTTCTGCAATATCCAAACAACTATCCCATGGCATACTTCTGTGTCC
AGCTCCTGGCAGTGGCTTCATATTTGGCCTTGTCGGACTTGTACATGTGA
GCAATCTCCGGTACAAGCGGGTCGTCAGGATTTGGGTCAGTAAGCAACGA
GCAGATAGAAAGCAGGACCTTTGAAATGGTAAGAGCTGGGCTCCACTGTT
CTTTAAGGATATCAAGACATATGCTTCCATTGCTGTTGATGTTTGGGTGG
AAGACCTTGGTCCTAAAAGCAACCTTTGGGGGCTTGAAGGGATAATCCGG
AGGAAAATGAATGGTAACCAAAAATACACCTCCTGCATATGGACTATCAG
GGGGGCCCATAATTGTTGCTTGCCAGTGGAACATGTCTTCAGCCACCGGA
CCGGCGCTGCAAGACGTGGGAGGATCCTTCTGCAAATCCTTGAGTT
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