CF832972

Overview
NameCF832972
Unique NameCF832972
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length674
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CF832972 vs. ExPASy Swiss-Prot
Match: SAR1B_PIG (GTP-binding protein SAR1b OS=Sus scrofa GN=SAR1B PE=2 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 3.869e-18
Identity = 47/174 (27.01%), Postives = 91/174 (52.30%), Query Frame = 2
Query:    2 KEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHRMLNEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLH------------SLRQRHWYIQSTCATSGEGLYEGLDWLSNNI 487
            K  +++ +GLD AGKTT+L+ LK   +   +PT+    E +    ++FT +D+GG  + R +W++Y     G++F+VD  D +R++E+++EL  ++ ++ + +  +L+  NK D P A++   + +  GL+             L  R   +        +G  EG  W++  I
Sbjct:   24 KTGKLVFLGLDNAGKTTLLHMLKDDRLGQHVPTLHPTSEELTIAGMTFTTFDLGGHVQARRVWKNYLPAINGIVFLVDCADHERLLESKEELDSLMTDETVANVPILILGNKIDRPEAISEERLREMFGLYGQTTGKGSVSLKELNARPLEVFMCSVLKRQGYGEGFRWMAQYI 197          
BLAST of CF832972 vs. ExPASy Swiss-Prot
Match: SAR1B_BOVIN (GTP-binding protein SAR1b OS=Bos taurus GN=SAR1B PE=2 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 3.869e-18
Identity = 47/174 (27.01%), Postives = 91/174 (52.30%), Query Frame = 2
Query:    2 KEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHRMLNEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLH------------SLRQRHWYIQSTCATSGEGLYEGLDWLSNNI 487
            K  +++ +GLD AGKTT+L+ LK   +   +PT+    E +    ++FT +D+GG  + R +W++Y     G++F+VD  D +R++E+++EL  ++ ++ + +  +L+  NK D P A++   + +  GL+             L  R   +        +G  EG  W++  I
Sbjct:   24 KTGKLVFLGLDNAGKTTLLHMLKDDRLGQHVPTLHPTSEELTIAGMTFTTFDLGGHVQARRVWKNYLPAINGIVFLVDCADHERLLESKEELDSLMTDETVANVPILILGNKIDRPEAISEERLREMFGLYGQTTGKGNVSLKELNARPLEVFMCSVLKRQGYGEGFRWMAQYI 197          
BLAST of CF832972 vs. ExPASy Swiss-Prot
Match: SAR1A_BRACM (GTP-binding protein SAR1A OS=Brassica campestris GN=SAR1A PE=2 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 3.869e-18
Identity = 56/176 (31.82%), Postives = 88/176 (50.00%), Query Frame = 2
Query:    2 KEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHRMLNEDELRDAVLLVFANKQDLPNAMNAAEITDKLGL--------------HSLRQRHWYIQSTCATSGEGLYEGLDWLSNNI 487
            KE +IL +GLD AGKTT+L+ LK   +V   PT     E +    I F  +D+GG    R +W+ Y+     ++++VD+ D++R  E++ EL  +L+++ L     L+  NK D+P A +  E+   LGL               ++R    ++ S     G G  EG  WLS  I
Sbjct:   19 KEAKILFLGLDNAGKTTLLHMLKDERLVQHQPTQHPTSEELSIGKIKFKAFDLGGHQIARRVWKDYYAKVDAVVYLVDAYDKERFAESKKELDALLSDESLATVPFLILGNKIDIPYAASEDELRYHLGLSNFTTGKGKVDLVGSNVRPLEVFMCSIVRKMGYG--EGFKWLSQYI 192          
BLAST of CF832972 vs. ExPASy Swiss-Prot
Match: SAR1A_PIG (GTP-binding protein SAR1a OS=Sus scrofa GN=SAR1A PE=2 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 5.052e-18
Identity = 48/174 (27.59%), Postives = 92/174 (52.87%), Query Frame = 2
Query:    2 KEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHRMLNEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLH------------SLRQRHWYIQSTCATSGEGLYEGLDWLSNNI 487
            K  +++ +GLD AGKTT+L+ LK   +   +PT+    E +    ++FT +D+GG ++ R +W++Y     G++F+VD  D  R++E++ EL+ ++ ++ + +  +L+  NK D  +A++  ++ +  GL+             L  R   +        +G  EG  WLS  I
Sbjct:   24 KSGKLVFLGLDNAGKTTLLHMLKDDRLGQHVPTLHPTSEELTIAGMTFTTFDLGGHEQARRVWKNYLPAINGIVFLVDCADHPRLMESKVELNALMTDETISNVPILILGNKIDRTDAISEEKLREIFGLYGQTTGKGNVTLKELNARPMEVFMCSVLKRQGYGEGFRWLSQYI 197          
BLAST of CF832972 vs. ExPASy Swiss-Prot
Match: SAR1A_BOVIN (GTP-binding protein SAR1a OS=Bos taurus GN=SAR1A PE=2 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 5.052e-18
Identity = 48/174 (27.59%), Postives = 92/174 (52.87%), Query Frame = 2
Query:    2 KEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHRMLNEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLH------------SLRQRHWYIQSTCATSGEGLYEGLDWLSNNI 487
            K  +++ +GLD AGKTT+L+ LK   +   +PT+    E +    ++FT +D+GG ++ R +W++Y     G++F+VD  D  R++E++ EL+ ++ ++ + +  +L+  NK D  +A++  ++ +  GL+             L  R   +        +G  EG  WLS  I
Sbjct:   24 KSGKLVFLGLDNAGKTTLLHMLKDDRLGQHVPTLHPTSEELTIAGMTFTTFDLGGHEQARRVWKNYLPAINGIVFLVDCADHPRLMESKVELNALMTDETISNVPILILGNKIDRTDAISEEKLREIFGLYGQTTGKGNVTLKELNARPMEVFMCSVLKRQGYGEGFRWLSQYI 197          
BLAST of CF832972 vs. ExPASy Swiss-Prot
Match: SAR1B_BRACM (GTP-binding protein SAR1B OS=Brassica campestris GN=SAR1B PE=2 SV=1)

HSP 1 Score: 90.8929 bits (224), Expect = 6.599e-18
Identity = 56/176 (31.82%), Postives = 85/176 (48.30%), Query Frame = 2
Query:    2 KEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHRMLNEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRQRHWYIQSTCATSG--------------EGLYEGLDWLSNNI 487
            KE +IL +GLD AGKTT+L+ LK   +V   PT     E +    I F  +D+GG    R +W+ Y+     ++++VD+ D++R  E++ EL  +L++D L     L+  NK D P A +  E+   LGL +       + +    SG               G  EG  WLS  I
Sbjct:   19 KEAKILFLGLDNAGKTTLLHMLKDERLVQHQPTQHPTSEELSIGKIKFKAFDLGGHQIARRVWKDYYAKVDAVVYLVDAYDKERFSESKKELDALLSDDALATVPFLILGNKIDNPYAASEDELRYHLGLTNFTTGKGKVTTAGGDSGVRPLEVFMCSIVRKMGYGEGFKWLSQYI 194          
BLAST of CF832972 vs. ExPASy Swiss-Prot
Match: SAR1A_ARATH (GTP-binding protein SAR1A OS=Arabidopsis thaliana GN=SAR1A PE=2 SV=1)

HSP 1 Score: 90.8929 bits (224), Expect = 6.599e-18
Identity = 46/130 (35.38%), Postives = 74/130 (56.92%), Query Frame = 2
Query:    2 KEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHRMLNEDELRDAVLLVFANKQDLPNAMNAAEITDKLGL 391
            KE +IL +GLD AGKTT+L+ LK   +V   PT     E +    I F  +D+GG    R +W+ Y+     ++++VD+ D++R  E++ EL  +L+++ L     L+  NK D+P A +  E+   LGL
Sbjct:   19 KEAKILFLGLDNAGKTTLLHMLKDERLVQHQPTQHPTSEELSIGKIKFKAFDLGGHQIARRVWKDYYAKVDAVVYLVDAYDKERFAESKKELDALLSDESLASVPFLILGNKIDIPYAASEDELRYHLGL 148          
BLAST of CF832972 vs. ExPASy Swiss-Prot
Match: SAR1B_ARATH (GTP-binding protein SAR1B OS=Arabidopsis thaliana GN=SAR1B PE=2 SV=1)

HSP 1 Score: 90.5077 bits (223), Expect = 8.618e-18
Identity = 56/176 (31.82%), Postives = 87/176 (49.43%), Query Frame = 2
Query:    2 KEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHRMLNEDELRDAVLLVFANKQDLPNAMNAAEITDKLGL--------------HSLRQRHWYIQSTCATSGEGLYEGLDWLSNNI 487
            KE +IL +GLD AGKTT+L+ LK   +V   PT     E +    I F  +D+GG    R +W+ Y+     ++++VD+ D++R  E++ EL  +L+++ L     L+  NK D+P A +  E+   LGL                +R    ++ S     G G  EG  WLS  I
Sbjct:   19 KEAKILFLGLDNAGKTTLLHMLKDERLVQHQPTQHPTSEELSIGKIKFKAFDLGGHQIARRVWKDYYAKVDAVVYLVDAYDKERFAESKRELDALLSDEALATVPFLILGNKIDIPYAASEDELRYHLGLTNFTTGKGKVTLGDSGVRPLEVFMCSIVRKMGYG--EGFKWLSQYI 192          
BLAST of CF832972 vs. ExPASy Swiss-Prot
Match: ARL10_HUMAN (ADP-ribosylation factor-like protein 10 OS=Homo sapiens GN=ARL10 PE=2 SV=1)

HSP 1 Score: 90.1225 bits (222), Expect = 1.126e-17
Identity = 51/134 (38.06%), Postives = 79/134 (58.96%), Query Frame = 2
Query:    2 KEMRILMVGLDAAGKTTILYKLK-LGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHRMLNEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSL 400
            ++  +L++GLD AGK+T L  L     +   IPT GFN   +  K+    + ++GG   +R  W+ +      L+FVVDS DR R+  AR ELH++L++D   D  ++V ANKQDL  AM+  E+  +LGL ++
Sbjct:   76 EQREVLVLGLDGAGKSTFLRVLSGKPPLEGHIPTWGFNSVRLPTKDFEVDLLEIGGSQNLRFYWKEFVSEVDVLVFVVDSADRLRLPWARQELHKLLDKDP--DLPVVVVANKQDLSEAMSMGELQRELGLQAI 207          
BLAST of CF832972 vs. ExPASy Swiss-Prot
Match: SAR1A_MOUSE (GTP-binding protein SAR1a OS=Mus musculus GN=Sar1a PE=2 SV=1)

HSP 1 Score: 89.7373 bits (221), Expect = 1.470e-17
Identity = 48/174 (27.59%), Postives = 91/174 (52.30%), Query Frame = 2
Query:    2 KEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHRMLNEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLH------------SLRQRHWYIQSTCATSGEGLYEGLDWLSNNI 487
            K  +++ +GLD AGKTT+L  LK   +   +PT+    E +    ++FT +D+GG ++ R +W++Y     G++F+VD  D  R++E++ EL+ ++ ++ + +  +L+  NK D  +A++  ++ +  GL+             L  R   +        +G  EG  WLS  I
Sbjct:   24 KSGKLVFLGLDNAGKTTLLQMLKDDRLGQHVPTLHPTSEELTIAGMTFTTFDLGGHEQARRVWKNYLPAINGIVFLVDCADHSRLMESKVELNALMTDETISNVPILILGNKIDRTDAISEEKLREIKGLYGQTTGKGNVTLKELNARPMEVFMCSVLKRQGYGEGFRWLSQYI 197          
The following BLAST results are available for this feature:
BLAST of CF832972 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 217
Match NameE-valueIdentityDescription
SAR1B_PIG3.869e-1827.01GTP-binding protein SAR1b OS=Sus scrofa GN=SAR1B P... [more]
SAR1B_BOVIN3.869e-1827.01GTP-binding protein SAR1b OS=Bos taurus GN=SAR1B P... [more]
SAR1A_BRACM3.869e-1831.82GTP-binding protein SAR1A OS=Brassica campestris G... [more]
SAR1A_PIG5.052e-1827.59GTP-binding protein SAR1a OS=Sus scrofa GN=SAR1A P... [more]
SAR1A_BOVIN5.052e-1827.59GTP-binding protein SAR1a OS=Bos taurus GN=SAR1A P... [more]
SAR1B_BRACM6.599e-1831.82GTP-binding protein SAR1B OS=Brassica campestris G... [more]
SAR1A_ARATH6.599e-1835.38GTP-binding protein SAR1A OS=Arabidopsis thaliana ... [more]
SAR1B_ARATH8.618e-1831.82GTP-binding protein SAR1B OS=Arabidopsis thaliana ... [more]
ARL10_HUMAN1.126e-1738.06ADP-ribosylation factor-like protein 10 OS=Homo sa... [more]
SAR1A_MOUSE1.470e-1727.59GTP-binding protein SAR1a OS=Mus musculus GN=Sar1a... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS02_02M03_r Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa02M03, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF832972 ID=CF832972; Name=CF832972; organism=Citrus sinensis; type=EST; length=674bp
GAAAGAGATGCGTATTCTGATGGTGGGTCTCGACGCTGCTGGTAAGACCA
CCATTTTGTACAAGCTCAAGCTCGGTGAGATTGTGACAACCATTCCTACC
ATTGGTTTCAACGTGGAGACTGTGGAATATAAGAACATTAGCTTCACTGT
TTGGGATGTTGGGGGTCAGGATAAGATTCGTCCTTTGTGGAGACATTACT
TCCAAAACACTCAAGGACTTATCTTTGTGGTTGATAGCAATGATCGTGAC
CGTGTGGTTGAAGCTAGAGATGAACTGCACCGGATGCTGAATGAGGATGA
GTTGAGGGATGCTGTGCTGCTTGTTTTTGCCAACAAGCAAGATCTTCCAA
ATGCCATGAATGCTGCAGAGATCACTGATAAGCTTGGTCTTCACTCACTT
CGCCAACGCCACTGGTACATCCAGAGCACATGTGCAACATCTGGGGAAGG
ACTCTATGAGGGTCTGGACTGGCTCTCCAACAACATCGCTAACAAGGCTT
AAGTGATTGGTCAAGTCTGAGCGACTCTTTGCGGCAGCATCTATGTGAAT
GATGTTACTATGCTTTTGTTTATTTTGTCGGGGATTCTTTTTGGGCTTTA
CTACCTACGTTCAGTAGAATGTATTTGTTTGTGTTAGATATTTGATTGGC
ACCATACTATACAGTTTTCTGCAT
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