CF832974

Overview
NameCF832974
Unique NameCF832974
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length850
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CF832974 vs. ExPASy Swiss-Prot
Match: METK_NEIG2 (S-adenosylmethionine synthetase OS=Neisseria gonorrhoeae (strain NCCP11945) GN=metK PE=3 SV=1)

HSP 1 Score: 137.117 bits (344), Expect = 1.193e-31
Identity = 71/131 (54.20%), Postives = 91/131 (69.47%), Query Frame = -1 
Query:  458 GRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVANGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 850
            GRKII+DTY            GKDP+KVDRS AY  R  AK+IVA GLA +C +QVSYAIGV EP S+ +D++GTGKI +++++ +V E FD RP  +   LDL R   G   K+AAYGHFGR++P+FTWE
Sbjct:  248 GRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYACRYVAKNIVAAGLATQCQIQVSYAIGVAEPTSISIDTFGTGKISEEKLIALVCEHFDLRPKGIVQMLDLLRPIYG---KSAAYGHFGREEPEFTWE 375
[Close]
BLAST of CF832974 vs. ExPASy Swiss-Prot
Match: METK_NEIG1 (S-adenosylmethionine synthetase OS=Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090) GN=metK PE=3 SV=2)

HSP 1 Score: 137.117 bits (344), Expect = 1.193e-31
Identity = 71/131 (54.20%), Postives = 91/131 (69.47%), Query Frame = -1 
Query:  458 GRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVANGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 850
            GRKII+DTY            GKDP+KVDRS AY  R  AK+IVA GLA +C +QVSYAIGV EP S+ +D++GTGKI +++++ +V E FD RP  +   LDL R   G   K+AAYGHFGR++P+FTWE
Sbjct:  248 GRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYACRYVAKNIVAAGLATQCQIQVSYAIGVAEPTSISIDTFGTGKISEEKLIALVCEHFDLRPKGIVQMLDLLRPIYG---KSAAYGHFGREEPEFTWE 375
[Close]
BLAST of CF832974 vs. ExPASy Swiss-Prot
Match: METK_GEOUR (S-adenosylmethionine synthetase OS=Geobacter uraniireducens (strain Rf4) GN=metK PE=3 SV=1)

HSP 1 Score: 137.117 bits (344), Expect = 1.193e-31
Identity = 73/131 (55.73%), Postives = 89/131 (67.94%), Query Frame = -1 
Query:  458 GRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVANGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 850
            GRKII+D+Y            GKDP+KVDRS AY+ R  AK++VA GL  +C VQV+YAIGV EP+SV VD+ GTGKIP   I +IV+E FD RP  +   LDL R     + KTAAYGHFGR+ P+FTWE
Sbjct:  248 GRKIIVDSYGGHGAHGGGAFSGKDPSKVDRSAAYMGRYVAKNLVAAGLCEKCEVQVAYAIGVAEPVSVMVDTSGTGKIPSARIAQIVREVFDLRPRAIIEQLDLLR---PIYKKTAAYGHFGRELPEFTWE 375
[Close]
BLAST of CF832974 vs. ExPASy Swiss-Prot
Match: METK_BURXL (S-adenosylmethionine synthetase OS=Burkholderia xenovorans (strain LB400) GN=metK PE=3 SV=1)

HSP 1 Score: 137.117 bits (344), Expect = 1.193e-31
Identity = 71/131 (54.20%), Postives = 91/131 (69.47%), Query Frame = -1 
Query:  458 GRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVANGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 850
            GRKII+DTY            GKDP+KVDRS AY  R  AK+IVA GLA RC++QVSYAIGV +P SV V+++GTG++ D  I ++V+E FD RP  +   LDL R     + K+AAYGHFGR++P+FTWE
Sbjct:  247 GRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLASRCLIQVSYAIGVAQPTSVMVNTFGTGRVSDAVITRLVQEHFDLRPKGIIQMLDLLR---PIYEKSAAYGHFGREEPEFTWE 374
[Close]
BLAST of CF832974 vs. ExPASy Swiss-Prot
Match: METK_AZOSE (S-adenosylmethionine synthetase OS=Azoarcus sp. (strain EbN1) GN=metK PE=3 SV=1)

HSP 1 Score: 137.117 bits (344), Expect = 1.193e-31
Identity = 71/131 (54.20%), Postives = 92/131 (70.23%), Query Frame = -1 
Query:  458 GRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVANGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 850
            GRKII+DTY            GKDP+KVDRS AY  R  AK+IVA GLA RC VQV+YAIGV  P+S+ ++++GTG+I D++I+++V+  FD RP  +   L+L R   G   KTAAYGHFGRD+P+FTWE
Sbjct:  247 GRKIIVDTYGGAAHHGGGAFSGKDPSKVDRSAAYAGRWVAKNIVAAGLADRCEVQVAYAIGVARPVSMMIETFGTGRITDEKIVELVQRHFDLRPKAIIQELNLLRPIYG---KTAAYGHFGRDEPEFTWE 374
[Close]
BLAST of CF832974 vs. ExPASy Swiss-Prot
Match: METK_POLNA (S-adenosylmethionine synthetase OS=Polaromonas naphthalenivorans (strain CJ2) GN=metK PE=3 SV=1)

HSP 1 Score: 136.732 bits (343), Expect = 1.558e-31
Identity = 72/131 (54.96%), Postives = 91/131 (69.47%), Query Frame = -1 
Query:  458 GRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVANGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 850
            GRKII+DTY            GKDP+KVDRS AY  R  AK+IVA GLAR+C +QV+YAIGV +P++V V + GTG IPD+++  +V+E FD RP  +   LDL R     + KTAAYGHFGRD+P+FTWE
Sbjct:  254 GRKIIVDTYGGACPHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLARQCQIQVAYAIGVAKPMNVTVYTEGTGVIPDEKLSALVQEHFDLRPNGIIQMLDLLR---PIYEKTAAYGHFGRDEPEFTWE 381
[Close]
BLAST of CF832974 vs. ExPASy Swiss-Prot
Match: METK_DELAS (S-adenosylmethionine synthetase OS=Delftia acidovorans (strain DSM 14801 / SPH-1) GN=metK PE=3 SV=1)

HSP 1 Score: 136.732 bits (343), Expect = 1.558e-31
Identity = 73/131 (55.73%), Postives = 88/131 (67.18%), Query Frame = -1 
Query:  458 GRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVANGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 850
            GRKII+DTY            GKDPTKVDRS AY  R  AK+IVA GLAR+C +QV+YAIGV  P+++ V + GTG IPD EI K+V   FD RP  +   LDL R     + KTAAYGHFGR++P+FTWE
Sbjct:  252 GRKIIVDTYGGACPHGGGAFSGKDPTKVDRSAAYAARYVAKNIVAAGLARQCQIQVAYAIGVARPMNITVYTEGTGVIPDAEIAKLVAAHFDLRPKGIIQMLDLLR---PIYSKTAAYGHFGREEPEFTWE 379
[Close]
BLAST of CF832974 vs. ExPASy Swiss-Prot
Match: METK_THENN (S-adenosylmethionine synthetase OS=Thermotoga neapolitana (strain ATCC 49049 / DSM 4359 / NS-E) GN=metK PE=3 SV=1)

HSP 1 Score: 136.346 bits (342), Expect = 2.035e-31
Identity = 66/131 (50.38%), Postives = 93/131 (70.99%), Query Frame = -1 
Query:  458 GRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVANGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 850
            GRKII+DTY            GKDPTKVDRS  Y+ R  AK++VA GLA + ++Q+SYAIGV +P+S+ +D++GT K+ ++++LK++ E FDFRPG +   L+L R     + KTAAYGHFGR++ +FTWE
Sbjct:  254 GRKIIVDTYGGWVPHGGGAFSGKDPTKVDRSAHYMARYVAKNVVAAGLADKFLIQLSYAIGVAKPVSILIDTFGTAKVDEEKLLKVITEIFDFRPGAIIKKLNLLR---PIYRKTAAYGHFGRNEEEFTWE 381
[Close]
BLAST of CF832974 vs. ExPASy Swiss-Prot
Match: METK_GEOMG (S-adenosylmethionine synthetase OS=Geobacter metallireducens (strain GS-15 / ATCC 53774 / DSM 7210) GN=metK PE=3 SV=1)

HSP 1 Score: 136.346 bits (342), Expect = 2.035e-31
Identity = 73/131 (55.73%), Postives = 89/131 (67.94%), Query Frame = -1 
Query:  458 GRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVANGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 850
            GRKII+D+Y            GKDP+KVDRS AY+ R  AK++VA GL  +C VQV+YAIGV EP+SV VD+ GTGKI  K I +IV+E FD RP  +   LDL R     + KTAAYGHFGR+ P+FTWE
Sbjct:  248 GRKIIVDSYGGHGAHGGGAFSGKDPSKVDRSAAYMGRYVAKNLVAAGLCEKCEVQVAYAIGVAEPVSVMVDTAGTGKISSKRIAEIVREVFDLRPRAIIEQLDLLR---PIYRKTAAYGHFGRELPEFTWE 375
[Close]
BLAST of CF832974 vs. ExPASy Swiss-Prot
Match: METK_BURVG (S-adenosylmethionine synthetase OS=Burkholderia vietnamiensis (strain G4 / LMG 22486) GN=metK PE=3 SV=1)

HSP 1 Score: 136.346 bits (342), Expect = 2.035e-31
Identity = 72/131 (54.96%), Postives = 90/131 (68.70%), Query Frame = -1 
Query:  458 GRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVANGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 850
            GRKII+DTY            GKDP+KVDRS AY  R  AK+IVA GLA R ++QVSYAIGV EP SV V+++GTG++ D  I K+V+E FD RP  +   LDL R     + KTAAYGHFGR++P+F+WE
Sbjct:  247 GRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLASRALIQVSYAIGVAEPTSVMVNTFGTGRVSDAVITKLVREHFDLRPKGIIKMLDLLR---PIYEKTAAYGHFGREEPEFSWE 374
The following BLAST results are available for this feature:
BLAST of CF832974 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
METK_NEIG21.193e-3154.20S-adenosylmethionine synthetase OS=Neisseria gonor... [more]
METK_NEIG11.193e-3154.20S-adenosylmethionine synthetase OS=Neisseria gonor... [more]
METK_GEOUR1.193e-3155.73S-adenosylmethionine synthetase OS=Geobacter urani... [more]
METK_BURXL1.193e-3154.20S-adenosylmethionine synthetase OS=Burkholderia xe... [more]
METK_AZOSE1.193e-3154.20S-adenosylmethionine synthetase OS=Azoarcus sp. (s... [more]
METK_POLNA1.558e-3154.96S-adenosylmethionine synthetase OS=Polaromonas nap... [more]
METK_DELAS1.558e-3155.73S-adenosylmethionine synthetase OS=Delftia acidovo... [more]
METK_THENN2.035e-3150.38S-adenosylmethionine synthetase OS=Thermotoga neap... [more]
METK_GEOMG2.035e-3155.73S-adenosylmethionine synthetase OS=Geobacter metal... [more]
METK_BURVG2.035e-3154.96S-adenosylmethionine synthetase OS=Burkholderia vi... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS02_02M06_f Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa02M06, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF832974 ID=CF832974; Name=CF832974; organism=Citrus sinensis; type=EST; length=850bp
TTTTTTTTTTTTTTTTAGGCAAACCGCACACACATAGAGTGATATCTAAT
AAGCAAAACAACTATTGGCTTTAGCCAAAGTTACAAATTAATACAACTCG
CTGCTGCCTTCATTCTACATTCCAAAAATAGTCGTAAGCAAAAGGAAGAA
TTTTACTAATTCCAGGCACCAGTTGAGCAAAATTCTCAATCGATATCACT
ACACAACAGTGGTGACATGGTACATTAAACCAAAACCAAAAAAGAAGTGG
GGAAAGAAGAAAAAGAAAGGAATTGAATGGGGAACATGTATGAGTTCTGA
ACACAGGAGATTTGCTTAGTATTACAAAGCAGCAGCAAGATAATATTCAA
ATTATTCATCCACTACCAATAGAAGCAAACTTGAACAGCAGGGATAGTTT
TCACTTTTCAACTCAAATTTAAGCTTGAGGCTTTTCCCACTTGAGGGGCT
TCACAACTTCCCAAGTGAAGTCTGGGTCATCCCTGCCAAAGTGTCCATAG
GCAGCAGTCTTCAAGAACCTGCCATTACCGCCCCTTTTGAGATCCAAATT
GATGGTCATCATTCCTGGCCTGAAATCAAAGCTTTCCTTCACAATTTTGA
GGATCTCCTTGTCAGGAATCTTGCCGGTTCCATATGAGTCCACAAAAACA
GACAATGGCTCAGGCACACCAATGGCATAGGAGACTTGGACAATGCACCT
GCGAGCAAGCCCGTTTGCAACAATGCTCTTAGCAGCCTGCCTAACAATGT
ATGCACCACTCCTATCCACCTTAGTAGGGTCTTTTCCAGAGAAAGCACCG
CCACCATGGGCTCCCCAGCCACCATAAGTGTCGATGATGATCTTACGTCC
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