CF832974

Overview
NameCF832974
Unique NameCF832974
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length850
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CF832974 vs. ExPASy Swiss-Prot
Match: METK_KLEP3 (S-adenosylmethionine synthetase OS=Klebsiella pneumoniae (strain 342) GN=metK PE=3 SV=1)

HSP 1 Score: 122.865 bits (307), Expect = 2.328e-27
Identity = 67/131 (51.15%), Postives = 86/131 (65.65%), Query Frame = -1 
Query:  458 GRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVANGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 850
            GRKII+DTY            GKDP+KVDRS AY  R  AK+IVA GLA RC +QVSYAIGV EP S+ V+++GT K+P +++  +V+E FD RP  +   LDL    +  + +TAAYGHFGR+   F WE
Sbjct:  244 GRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFFDLRPYGLIQMLDLL---HPIYKETAAYGHFGRE--HFPWE 369
[Close]
BLAST of CF832974 vs. ExPASy Swiss-Prot
Match: METK_ESCF3 (S-adenosylmethionine synthetase OS=Escherichia fergusonii (strain ATCC 35469 / DSM 13698 / CDC 0568-73) GN=metK PE=3 SV=1)

HSP 1 Score: 122.865 bits (307), Expect = 2.328e-27
Identity = 67/131 (51.15%), Postives = 86/131 (65.65%), Query Frame = -1 
Query:  458 GRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVANGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 850
            GRKII+DTY            GKDP+KVDRS AY  R  AK+IVA GLA RC +QVSYAIGV EP S+ V+++GT K+P +++  +V+E FD RP  +   LDL    +  + +TAAYGHFGR+   F WE
Sbjct:  244 GRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFFDLRPYGLIQMLDLL---HPIYKETAAYGHFGRE--HFPWE 369
[Close]
BLAST of CF832974 vs. ExPASy Swiss-Prot
Match: METK_ECOSM (S-adenosylmethionine synthetase OS=Escherichia coli (strain SMS-3-5 / SECEC) GN=metK PE=3 SV=1)

HSP 1 Score: 122.865 bits (307), Expect = 2.328e-27
Identity = 67/131 (51.15%), Postives = 86/131 (65.65%), Query Frame = -1 
Query:  458 GRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVANGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 850
            GRKII+DTY            GKDP+KVDRS AY  R  AK+IVA GLA RC +QVSYAIGV EP S+ V+++GT K+P +++  +V+E FD RP  +   LDL    +  + +TAAYGHFGR+   F WE
Sbjct:  244 GRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFFDLRPYGLIQMLDLL---HPIYKETAAYGHFGRE--HFPWE 369
[Close]
BLAST of CF832974 vs. ExPASy Swiss-Prot
Match: METK_ECOSE (S-adenosylmethionine synthetase OS=Escherichia coli (strain SE11) GN=metK PE=3 SV=1)

HSP 1 Score: 122.865 bits (307), Expect = 2.328e-27
Identity = 67/131 (51.15%), Postives = 86/131 (65.65%), Query Frame = -1 
Query:  458 GRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVANGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 850
            GRKII+DTY            GKDP+KVDRS AY  R  AK+IVA GLA RC +QVSYAIGV EP S+ V+++GT K+P +++  +V+E FD RP  +   LDL    +  + +TAAYGHFGR+   F WE
Sbjct:  244 GRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFFDLRPYGLIQMLDLL---HPIYKETAAYGHFGRE--HFPWE 369
[Close]
BLAST of CF832974 vs. ExPASy Swiss-Prot
Match: METK_ECOLU (S-adenosylmethionine synthetase OS=Escherichia coli O17:K52:H18 (strain UMN026 / ExPEC) GN=metK PE=3 SV=1)

HSP 1 Score: 122.865 bits (307), Expect = 2.328e-27
Identity = 67/131 (51.15%), Postives = 86/131 (65.65%), Query Frame = -1 
Query:  458 GRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVANGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 850
            GRKII+DTY            GKDP+KVDRS AY  R  AK+IVA GLA RC +QVSYAIGV EP S+ V+++GT K+P +++  +V+E FD RP  +   LDL    +  + +TAAYGHFGR+   F WE
Sbjct:  244 GRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFFDLRPYGLIQMLDLL---HPIYKETAAYGHFGRE--HFPWE 369
[Close]
BLAST of CF832974 vs. ExPASy Swiss-Prot
Match: METK_ECOLI (S-adenosylmethionine synthetase OS=Escherichia coli (strain K12) GN=metK PE=1 SV=2)

HSP 1 Score: 122.865 bits (307), Expect = 2.328e-27
Identity = 67/131 (51.15%), Postives = 86/131 (65.65%), Query Frame = -1 
Query:  458 GRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVANGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 850
            GRKII+DTY            GKDP+KVDRS AY  R  AK+IVA GLA RC +QVSYAIGV EP S+ V+++GT K+P +++  +V+E FD RP  +   LDL    +  + +TAAYGHFGR+   F WE
Sbjct:  244 GRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFFDLRPYGLIQMLDLL---HPIYKETAAYGHFGRE--HFPWE 369
[Close]
BLAST of CF832974 vs. ExPASy Swiss-Prot
Match: METK_ECOLC (S-adenosylmethionine synthetase OS=Escherichia coli (strain ATCC 8739 / DSM 1576 / Crooks) GN=metK PE=3 SV=1)

HSP 1 Score: 122.865 bits (307), Expect = 2.328e-27
Identity = 67/131 (51.15%), Postives = 86/131 (65.65%), Query Frame = -1 
Query:  458 GRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVANGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 850
            GRKII+DTY            GKDP+KVDRS AY  R  AK+IVA GLA RC +QVSYAIGV EP S+ V+++GT K+P +++  +V+E FD RP  +   LDL    +  + +TAAYGHFGR+   F WE
Sbjct:  244 GRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFFDLRPYGLIQMLDLL---HPIYKETAAYGHFGRE--HFPWE 369
[Close]
BLAST of CF832974 vs. ExPASy Swiss-Prot
Match: METK_ECOL6 (S-adenosylmethionine synthetase OS=Escherichia coli O6 GN=metK PE=3 SV=2)

HSP 1 Score: 122.865 bits (307), Expect = 2.328e-27
Identity = 67/131 (51.15%), Postives = 86/131 (65.65%), Query Frame = -1 
Query:  458 GRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVANGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 850
            GRKII+DTY            GKDP+KVDRS AY  R  AK+IVA GLA RC +QVSYAIGV EP S+ V+++GT K+P +++  +V+E FD RP  +   LDL    +  + +TAAYGHFGR+   F WE
Sbjct:  244 GRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFFDLRPYGLIQMLDLL---HPIYKETAAYGHFGRE--HFPWE 369
[Close]
BLAST of CF832974 vs. ExPASy Swiss-Prot
Match: METK_ECOL5 (S-adenosylmethionine synthetase OS=Escherichia coli O6:K15:H31 (strain 536 / UPEC) GN=metK PE=3 SV=1)

HSP 1 Score: 122.865 bits (307), Expect = 2.328e-27
Identity = 67/131 (51.15%), Postives = 86/131 (65.65%), Query Frame = -1 
Query:  458 GRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVANGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 850
            GRKII+DTY            GKDP+KVDRS AY  R  AK+IVA GLA RC +QVSYAIGV EP S+ V+++GT K+P +++  +V+E FD RP  +   LDL    +  + +TAAYGHFGR+   F WE
Sbjct:  244 GRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFFDLRPYGLIQMLDLL---HPIYKETAAYGHFGRE--HFPWE 369
[Close]
BLAST of CF832974 vs. ExPASy Swiss-Prot
Match: METK_ECOHS (S-adenosylmethionine synthetase OS=Escherichia coli O9:H4 (strain HS) GN=metK PE=3 SV=1)

HSP 1 Score: 122.865 bits (307), Expect = 2.328e-27
Identity = 67/131 (51.15%), Postives = 86/131 (65.65%), Query Frame = -1 
Query:  458 GRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVANGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 850
            GRKII+DTY            GKDP+KVDRS AY  R  AK+IVA GLA RC +QVSYAIGV EP S+ V+++GT K+P +++  +V+E FD RP  +   LDL    +  + +TAAYGHFGR+   F WE
Sbjct:  244 GRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFFDLRPYGLIQMLDLL---HPIYKETAAYGHFGRE--HFPWE 369
The following BLAST results are available for this feature:
BLAST of CF832974 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
METK_KLEP32.328e-2751.15S-adenosylmethionine synthetase OS=Klebsiella pneu... [more]
METK_ESCF32.328e-2751.15S-adenosylmethionine synthetase OS=Escherichia fer... [more]
METK_ECOSM2.328e-2751.15S-adenosylmethionine synthetase OS=Escherichia col... [more]
METK_ECOSE2.328e-2751.15S-adenosylmethionine synthetase OS=Escherichia col... [more]
METK_ECOLU2.328e-2751.15S-adenosylmethionine synthetase OS=Escherichia col... [more]
METK_ECOLI2.328e-2751.15S-adenosylmethionine synthetase OS=Escherichia col... [more]
METK_ECOLC2.328e-2751.15S-adenosylmethionine synthetase OS=Escherichia col... [more]
METK_ECOL62.328e-2751.15S-adenosylmethionine synthetase OS=Escherichia col... [more]
METK_ECOL52.328e-2751.15S-adenosylmethionine synthetase OS=Escherichia col... [more]
METK_ECOHS2.328e-2751.15S-adenosylmethionine synthetase OS=Escherichia col... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS02_02M06_f Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa02M06, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF832974 ID=CF832974; Name=CF832974; organism=Citrus sinensis; type=EST; length=850bp
TTTTTTTTTTTTTTTTAGGCAAACCGCACACACATAGAGTGATATCTAAT
AAGCAAAACAACTATTGGCTTTAGCCAAAGTTACAAATTAATACAACTCG
CTGCTGCCTTCATTCTACATTCCAAAAATAGTCGTAAGCAAAAGGAAGAA
TTTTACTAATTCCAGGCACCAGTTGAGCAAAATTCTCAATCGATATCACT
ACACAACAGTGGTGACATGGTACATTAAACCAAAACCAAAAAAGAAGTGG
GGAAAGAAGAAAAAGAAAGGAATTGAATGGGGAACATGTATGAGTTCTGA
ACACAGGAGATTTGCTTAGTATTACAAAGCAGCAGCAAGATAATATTCAA
ATTATTCATCCACTACCAATAGAAGCAAACTTGAACAGCAGGGATAGTTT
TCACTTTTCAACTCAAATTTAAGCTTGAGGCTTTTCCCACTTGAGGGGCT
TCACAACTTCCCAAGTGAAGTCTGGGTCATCCCTGCCAAAGTGTCCATAG
GCAGCAGTCTTCAAGAACCTGCCATTACCGCCCCTTTTGAGATCCAAATT
GATGGTCATCATTCCTGGCCTGAAATCAAAGCTTTCCTTCACAATTTTGA
GGATCTCCTTGTCAGGAATCTTGCCGGTTCCATATGAGTCCACAAAAACA
GACAATGGCTCAGGCACACCAATGGCATAGGAGACTTGGACAATGCACCT
GCGAGCAAGCCCGTTTGCAACAATGCTCTTAGCAGCCTGCCTAACAATGT
ATGCACCACTCCTATCCACCTTAGTAGGGTCTTTTCCAGAGAAAGCACCG
CCACCATGGGCTCCCCAGCCACCATAAGTGTCGATGATGATCTTACGTCC
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