CF832974

Overview
NameCF832974
Unique NameCF832974
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length850
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig1234 contig Csv1_Contig1234:998..1846. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CF832974 vs. ExPASy Swiss-Prot
Match: METK_LISW6 (S-adenosylmethionine synthetase OS=Listeria welshimeri serovar 6b (strain ATCC 35897 / DSM 20650 / SLCC5334) GN=metK PE=3 SV=1)

HSP 1 Score: 122.094 bits (305), Expect = 3.971e-27
Identity = 65/131 (49.62%), Postives = 84/131 (64.12%), Query Frame = -1 
Query:  458 GRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVANGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 850
            GRKII+DTY            GKDPTKVDRSGAY  R  AK+IVA GLA++  VQ++YAIGV  P+S+ +D+YGT    ++E++  V   FD RP  +   LDL+R     + +TAA+GHFGR D D  WE
Sbjct:  258 GRKIIVDTYGGYARHGGGAFSGKDPTKVDRSGAYAARYVAKNIVAAGLAKKVEVQLAYAIGVARPVSISIDTYGTSDYSEQELIDGVNALFDLRPAGIIHMLDLRR---PIYRQTAAFGHFGRSDLDLPWE 385
[Close]
BLAST of CF832974 vs. ExPASy Swiss-Prot
Match: METK_HELHP (S-adenosylmethionine synthetase OS=Helicobacter hepaticus GN=metK PE=3 SV=1)

HSP 1 Score: 122.094 bits (305), Expect = 3.971e-27
Identity = 65/131 (49.62%), Postives = 86/131 (65.65%), Query Frame = -1 
Query:  458 GRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVANGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 850
            GRKII+DTY            GKDP+KVDRS AY  R  AK++VA+G+  + IVQV+YAIGV EP+S+ VD+ GTGK+ D ++ + VK  F   P  +  +LDL R     + KTAAYGHFGR+  +F+WE
Sbjct:  244 GRKIIVDTYGGSCPHGGGAFSGKDPSKVDRSAAYAARYVAKNLVASGVCDKAIVQVAYAIGVVEPVSILVDTQGTGKVEDSKLTECVKAVFRLTPKGIIESLDLLR---PIYRKTAAYGHFGRELNEFSWE 371
[Close]
BLAST of CF832974 vs. ExPASy Swiss-Prot
Match: METK_ENT38 (S-adenosylmethionine synthetase OS=Enterobacter sp. (strain 638) GN=metK PE=3 SV=1)

HSP 1 Score: 122.094 bits (305), Expect = 3.971e-27
Identity = 67/131 (51.15%), Postives = 86/131 (65.65%), Query Frame = -1 
Query:  458 GRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVANGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 850
            GRKII+DTY            GKDP+KVDRS AY  R  AK+IVA GLA RC +QVSYAIGV EP S+ V+++GT K+P +++  +V+E FD RP  +   LDL    +  + +TAAYGHFGR+   F WE
Sbjct:  244 GRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKLPTEQLTLLVREFFDLRPYGLIQMLDLL---HPIYKETAAYGHFGRE--HFPWE 369
[Close]
BLAST of CF832974 vs. ExPASy Swiss-Prot
Match: METK_YERPY (S-adenosylmethionine synthetase OS=Yersinia pseudotuberculosis serotype O:3 (strain YPIII) GN=metK PE=3 SV=1)

HSP 1 Score: 121.709 bits (304), Expect = 5.186e-27
Identity = 68/131 (51.91%), Postives = 85/131 (64.89%), Query Frame = -1 
Query:  458 GRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVANGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 850
            GRKII+DTY            GKDP+KVDRS AY  R  AK+IVA GLA RC +QVSYAIGV EP S+ V+++GT KIP  ++  +V+E FD RP  +   LDL    +  + +TAAYGHFGR+   F WE
Sbjct:  244 GRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVEAFGTEKIPADQLTLLVREFFDLRPYGLIKMLDLL---HPIYRETAAYGHFGRE--HFPWE 369
[Close]
BLAST of CF832974 vs. ExPASy Swiss-Prot
Match: METK_YERPS (S-adenosylmethionine synthetase OS=Yersinia pseudotuberculosis GN=metK PE=3 SV=1)

HSP 1 Score: 121.709 bits (304), Expect = 5.186e-27
Identity = 68/131 (51.91%), Postives = 85/131 (64.89%), Query Frame = -1 
Query:  458 GRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVANGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 850
            GRKII+DTY            GKDP+KVDRS AY  R  AK+IVA GLA RC +QVSYAIGV EP S+ V+++GT KIP  ++  +V+E FD RP  +   LDL    +  + +TAAYGHFGR+   F WE
Sbjct:  244 GRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVEAFGTEKIPADQLTLLVREFFDLRPYGLIKMLDLL---HPIYRETAAYGHFGRE--HFPWE 369
[Close]
BLAST of CF832974 vs. ExPASy Swiss-Prot
Match: METK_YERPP (S-adenosylmethionine synthetase OS=Yersinia pestis (strain Pestoides F) GN=metK PE=3 SV=1)

HSP 1 Score: 121.709 bits (304), Expect = 5.186e-27
Identity = 68/131 (51.91%), Postives = 85/131 (64.89%), Query Frame = -1 
Query:  458 GRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVANGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 850
            GRKII+DTY            GKDP+KVDRS AY  R  AK+IVA GLA RC +QVSYAIGV EP S+ V+++GT KIP  ++  +V+E FD RP  +   LDL    +  + +TAAYGHFGR+   F WE
Sbjct:  244 GRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVEAFGTEKIPADQLTLLVREFFDLRPYGLIKMLDLL---HPIYRETAAYGHFGRE--HFPWE 369
[Close]
BLAST of CF832974 vs. ExPASy Swiss-Prot
Match: METK_YERPN (S-adenosylmethionine synthetase OS=Yersinia pestis bv. Antiqua (strain Nepal516) GN=metK PE=3 SV=1)

HSP 1 Score: 121.709 bits (304), Expect = 5.186e-27
Identity = 68/131 (51.91%), Postives = 85/131 (64.89%), Query Frame = -1 
Query:  458 GRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVANGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 850
            GRKII+DTY            GKDP+KVDRS AY  R  AK+IVA GLA RC +QVSYAIGV EP S+ V+++GT KIP  ++  +V+E FD RP  +   LDL    +  + +TAAYGHFGR+   F WE
Sbjct:  244 GRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVEAFGTEKIPADQLTLLVREFFDLRPYGLIKMLDLL---HPIYRETAAYGHFGRE--HFPWE 369
[Close]
BLAST of CF832974 vs. ExPASy Swiss-Prot
Match: METK_YERPG (S-adenosylmethionine synthetase OS=Yersinia pestis bv. Antiqua (strain Angola) GN=metK PE=3 SV=1)

HSP 1 Score: 121.709 bits (304), Expect = 5.186e-27
Identity = 68/131 (51.91%), Postives = 85/131 (64.89%), Query Frame = -1 
Query:  458 GRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVANGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 850
            GRKII+DTY            GKDP+KVDRS AY  R  AK+IVA GLA RC +QVSYAIGV EP S+ V+++GT KIP  ++  +V+E FD RP  +   LDL    +  + +TAAYGHFGR+   F WE
Sbjct:  244 GRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVEAFGTEKIPADQLTLLVREFFDLRPYGLIKMLDLL---HPIYRETAAYGHFGRE--HFPWE 369
[Close]
BLAST of CF832974 vs. ExPASy Swiss-Prot
Match: METK_YERPE (S-adenosylmethionine synthetase OS=Yersinia pestis GN=metK PE=3 SV=1)

HSP 1 Score: 121.709 bits (304), Expect = 5.186e-27
Identity = 68/131 (51.91%), Postives = 85/131 (64.89%), Query Frame = -1 
Query:  458 GRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVANGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 850
            GRKII+DTY            GKDP+KVDRS AY  R  AK+IVA GLA RC +QVSYAIGV EP S+ V+++GT KIP  ++  +V+E FD RP  +   LDL    +  + +TAAYGHFGR+   F WE
Sbjct:  244 GRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVEAFGTEKIPADQLTLLVREFFDLRPYGLIKMLDLL---HPIYRETAAYGHFGRE--HFPWE 369
[Close]
BLAST of CF832974 vs. ExPASy Swiss-Prot
Match: METK_YERPB (S-adenosylmethionine synthetase OS=Yersinia pseudotuberculosis serotype IB (strain PB1/+) GN=metK PE=3 SV=1)

HSP 1 Score: 121.709 bits (304), Expect = 5.186e-27
Identity = 68/131 (51.91%), Postives = 85/131 (64.89%), Query Frame = -1 
Query:  458 GRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVANGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 850
            GRKII+DTY            GKDP+KVDRS AY  R  AK+IVA GLA RC +QVSYAIGV EP S+ V+++GT KIP  ++  +V+E FD RP  +   LDL    +  + +TAAYGHFGR+   F WE
Sbjct:  244 GRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVEAFGTEKIPADQLTLLVREFFDLRPYGLIKMLDLL---HPIYRETAAYGHFGRE--HFPWE 369
The following BLAST results are available for this feature:
BLAST of CF832974 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
METK_LISW63.971e-2749.62S-adenosylmethionine synthetase OS=Listeria welshi... [more]
METK_HELHP3.971e-2749.62S-adenosylmethionine synthetase OS=Helicobacter he... [more]
METK_ENT383.971e-2751.15S-adenosylmethionine synthetase OS=Enterobacter sp... [more]
METK_YERPY5.186e-2751.91S-adenosylmethionine synthetase OS=Yersinia pseudo... [more]
METK_YERPS5.186e-2751.91S-adenosylmethionine synthetase OS=Yersinia pseudo... [more]
METK_YERPP5.186e-2751.91S-adenosylmethionine synthetase OS=Yersinia pestis... [more]
METK_YERPN5.186e-2751.91S-adenosylmethionine synthetase OS=Yersinia pestis... [more]
METK_YERPG5.186e-2751.91S-adenosylmethionine synthetase OS=Yersinia pestis... [more]
METK_YERPE5.186e-2751.91S-adenosylmethionine synthetase OS=Yersinia pestis... [more]
METK_YERPB5.186e-2751.91S-adenosylmethionine synthetase OS=Yersinia pseudo... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS02_02M06_f Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa02M06, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF832974 ID=CF832974; Name=CF832974; organism=Citrus sinensis; type=EST; length=850bp
TTTTTTTTTTTTTTTTAGGCAAACCGCACACACATAGAGTGATATCTAAT
AAGCAAAACAACTATTGGCTTTAGCCAAAGTTACAAATTAATACAACTCG
CTGCTGCCTTCATTCTACATTCCAAAAATAGTCGTAAGCAAAAGGAAGAA
TTTTACTAATTCCAGGCACCAGTTGAGCAAAATTCTCAATCGATATCACT
ACACAACAGTGGTGACATGGTACATTAAACCAAAACCAAAAAAGAAGTGG
GGAAAGAAGAAAAAGAAAGGAATTGAATGGGGAACATGTATGAGTTCTGA
ACACAGGAGATTTGCTTAGTATTACAAAGCAGCAGCAAGATAATATTCAA
ATTATTCATCCACTACCAATAGAAGCAAACTTGAACAGCAGGGATAGTTT
TCACTTTTCAACTCAAATTTAAGCTTGAGGCTTTTCCCACTTGAGGGGCT
TCACAACTTCCCAAGTGAAGTCTGGGTCATCCCTGCCAAAGTGTCCATAG
GCAGCAGTCTTCAAGAACCTGCCATTACCGCCCCTTTTGAGATCCAAATT
GATGGTCATCATTCCTGGCCTGAAATCAAAGCTTTCCTTCACAATTTTGA
GGATCTCCTTGTCAGGAATCTTGCCGGTTCCATATGAGTCCACAAAAACA
GACAATGGCTCAGGCACACCAATGGCATAGGAGACTTGGACAATGCACCT
GCGAGCAAGCCCGTTTGCAACAATGCTCTTAGCAGCCTGCCTAACAATGT
ATGCACCACTCCTATCCACCTTAGTAGGGTCTTTTCCAGAGAAAGCACCG
CCACCATGGGCTCCCCAGCCACCATAAGTGTCGATGATGATCTTACGTCC
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